Amino acid dipepetide frequency for Mycobacterium phage OwlsT2W

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.607AlaAla: 13.607 ± 1.74
1.277AlaCys: 1.277 ± 0.348
6.72AlaAsp: 6.72 ± 0.507
7.887AlaGlu: 7.887 ± 0.835
2.999AlaPhe: 2.999 ± 0.427
9.109AlaGly: 9.109 ± 1.213
1.999AlaHis: 1.999 ± 0.385
4.166AlaIle: 4.166 ± 0.446
4.277AlaLys: 4.277 ± 0.416
7.887AlaLeu: 7.887 ± 0.763
2.721AlaMet: 2.721 ± 0.431
3.055AlaAsn: 3.055 ± 0.429
5.276AlaPro: 5.276 ± 0.505
3.888AlaGln: 3.888 ± 0.526
6.498AlaArg: 6.498 ± 0.624
6.165AlaSer: 6.165 ± 0.66
6.665AlaThr: 6.665 ± 0.532
7.165AlaVal: 7.165 ± 0.607
2.444AlaTrp: 2.444 ± 0.412
2.666AlaTyr: 2.666 ± 0.394
0.0AlaXaa: 0.0 ± 0.0
Cys
1.277CysAla: 1.277 ± 0.286
0.056CysCys: 0.056 ± 0.048
1.0CysAsp: 1.0 ± 0.245
0.944CysGlu: 0.944 ± 0.237
0.167CysPhe: 0.167 ± 0.092
1.277CysGly: 1.277 ± 0.306
0.167CysHis: 0.167 ± 0.093
0.333CysIle: 0.333 ± 0.177
0.5CysLys: 0.5 ± 0.181
0.889CysLeu: 0.889 ± 0.242
0.222CysMet: 0.222 ± 0.109
0.611CysAsn: 0.611 ± 0.192
1.055CysPro: 1.055 ± 0.283
0.444CysGln: 0.444 ± 0.185
1.055CysArg: 1.055 ± 0.287
0.611CysSer: 0.611 ± 0.19
0.889CysThr: 0.889 ± 0.278
0.5CysVal: 0.5 ± 0.144
0.389CysTrp: 0.389 ± 0.148
0.167CysTyr: 0.167 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
6.776AspAla: 6.776 ± 0.54
0.833AspCys: 0.833 ± 0.247
3.888AspAsp: 3.888 ± 0.606
3.888AspGlu: 3.888 ± 0.445
1.444AspPhe: 1.444 ± 0.243
6.276AspGly: 6.276 ± 0.62
1.277AspHis: 1.277 ± 0.254
2.222AspIle: 2.222 ± 0.316
1.833AspLys: 1.833 ± 0.309
6.109AspLeu: 6.109 ± 0.568
1.444AspMet: 1.444 ± 0.293
2.055AspAsn: 2.055 ± 0.327
4.443AspPro: 4.443 ± 0.617
2.61AspGln: 2.61 ± 0.402
4.999AspArg: 4.999 ± 0.646
3.499AspSer: 3.499 ± 0.546
3.777AspThr: 3.777 ± 0.5
4.943AspVal: 4.943 ± 0.569
1.277AspTrp: 1.277 ± 0.233
1.999AspTyr: 1.999 ± 0.356
0.0AspXaa: 0.0 ± 0.0
Glu
6.054GluAla: 6.054 ± 0.616
1.055GluCys: 1.055 ± 0.294
3.61GluAsp: 3.61 ± 0.394
2.555GluGlu: 2.555 ± 0.407
2.222GluPhe: 2.222 ± 0.331
3.166GluGly: 3.166 ± 0.404
1.277GluHis: 1.277 ± 0.305
2.666GluIle: 2.666 ± 0.431
2.055GluLys: 2.055 ± 0.357
5.443GluLeu: 5.443 ± 0.715
1.5GluMet: 1.5 ± 0.249
1.555GluAsn: 1.555 ± 0.258
3.221GluPro: 3.221 ± 0.475
2.61GluGln: 2.61 ± 0.404
4.999GluArg: 4.999 ± 0.591
3.11GluSer: 3.11 ± 0.459
3.999GluThr: 3.999 ± 0.599
3.888GluVal: 3.888 ± 0.552
1.166GluTrp: 1.166 ± 0.284
1.333GluTyr: 1.333 ± 0.295
0.0GluXaa: 0.0 ± 0.0
Phe
2.61PheAla: 2.61 ± 0.341
0.278PheCys: 0.278 ± 0.121
2.388PheAsp: 2.388 ± 0.351
1.777PheGlu: 1.777 ± 0.29
0.833PhePhe: 0.833 ± 0.225
3.166PheGly: 3.166 ± 0.708
0.5PheHis: 0.5 ± 0.188
1.277PheIle: 1.277 ± 0.364
1.166PheLys: 1.166 ± 0.26
2.111PheLeu: 2.111 ± 0.32
0.666PheMet: 0.666 ± 0.176
1.277PheAsn: 1.277 ± 0.335
1.611PhePro: 1.611 ± 0.3
1.0PheGln: 1.0 ± 0.314
1.389PheArg: 1.389 ± 0.267
1.389PheSer: 1.389 ± 0.276
2.111PheThr: 2.111 ± 0.309
1.833PheVal: 1.833 ± 0.296
0.389PheTrp: 0.389 ± 0.137
0.833PheTyr: 0.833 ± 0.247
0.0PheXaa: 0.0 ± 0.0
Gly
9.775GlyAla: 9.775 ± 1.115
1.111GlyCys: 1.111 ± 0.278
6.276GlyAsp: 6.276 ± 0.565
3.666GlyGlu: 3.666 ± 0.489
2.777GlyPhe: 2.777 ± 0.393
10.664GlyGly: 10.664 ± 2.084
1.888GlyHis: 1.888 ± 0.328
4.443GlyIle: 4.443 ± 0.647
2.61GlyLys: 2.61 ± 0.355
5.443GlyLeu: 5.443 ± 0.521
2.166GlyMet: 2.166 ± 0.517
2.777GlyAsn: 2.777 ± 0.387
3.943GlyPro: 3.943 ± 0.533
2.055GlyGln: 2.055 ± 0.516
5.054GlyArg: 5.054 ± 0.626
6.665GlySer: 6.665 ± 1.042
6.887GlyThr: 6.887 ± 0.714
5.443GlyVal: 5.443 ± 0.657
2.277GlyTrp: 2.277 ± 0.368
2.388GlyTyr: 2.388 ± 0.438
0.0GlyXaa: 0.0 ± 0.0
His
1.722HisAla: 1.722 ± 0.325
0.389HisCys: 0.389 ± 0.169
1.055HisAsp: 1.055 ± 0.262
1.222HisGlu: 1.222 ± 0.264
0.444HisPhe: 0.444 ± 0.144
2.055HisGly: 2.055 ± 0.355
0.722HisHis: 0.722 ± 0.267
1.389HisIle: 1.389 ± 0.306
1.055HisLys: 1.055 ± 0.267
1.666HisLeu: 1.666 ± 0.369
0.722HisMet: 0.722 ± 0.268
0.944HisAsn: 0.944 ± 0.231
1.444HisPro: 1.444 ± 0.251
0.722HisGln: 0.722 ± 0.202
1.444HisArg: 1.444 ± 0.301
0.944HisSer: 0.944 ± 0.215
1.444HisThr: 1.444 ± 0.344
1.611HisVal: 1.611 ± 0.377
0.444HisTrp: 0.444 ± 0.142
0.833HisTyr: 0.833 ± 0.251
0.0HisXaa: 0.0 ± 0.0
Ile
4.999IleAla: 4.999 ± 0.53
0.555IleCys: 0.555 ± 0.211
3.721IleAsp: 3.721 ± 0.461
2.666IleGlu: 2.666 ± 0.447
0.611IlePhe: 0.611 ± 0.219
3.721IleGly: 3.721 ± 0.512
1.611IleHis: 1.611 ± 0.339
1.277IleIle: 1.277 ± 0.318
1.166IleLys: 1.166 ± 0.276
2.222IleLeu: 2.222 ± 0.335
0.333IleMet: 0.333 ± 0.124
2.166IleAsn: 2.166 ± 0.345
2.888IlePro: 2.888 ± 0.433
1.555IleGln: 1.555 ± 0.281
2.944IleArg: 2.944 ± 0.418
1.999IleSer: 1.999 ± 0.443
4.166IleThr: 4.166 ± 0.531
3.499IleVal: 3.499 ± 0.405
1.0IleTrp: 1.0 ± 0.279
0.778IleTyr: 0.778 ± 0.193
0.0IleXaa: 0.0 ± 0.0
Lys
3.555LysAla: 3.555 ± 0.457
0.444LysCys: 0.444 ± 0.179
1.722LysAsp: 1.722 ± 0.315
1.055LysGlu: 1.055 ± 0.218
1.222LysPhe: 1.222 ± 0.237
2.499LysGly: 2.499 ± 0.387
1.166LysHis: 1.166 ± 0.307
0.722LysIle: 0.722 ± 0.212
1.5LysLys: 1.5 ± 0.36
3.11LysLeu: 3.11 ± 0.462
1.055LysMet: 1.055 ± 0.213
0.778LysAsn: 0.778 ± 0.216
2.833LysPro: 2.833 ± 0.469
1.389LysGln: 1.389 ± 0.24
2.61LysArg: 2.61 ± 0.395
1.999LysSer: 1.999 ± 0.336
2.444LysThr: 2.444 ± 0.366
2.277LysVal: 2.277 ± 0.418
1.055LysTrp: 1.055 ± 0.264
0.722LysTyr: 0.722 ± 0.224
0.0LysXaa: 0.0 ± 0.0
Leu
7.498LeuAla: 7.498 ± 0.748
0.833LeuCys: 0.833 ± 0.29
4.999LeuAsp: 4.999 ± 0.647
3.999LeuGlu: 3.999 ± 0.484
2.277LeuPhe: 2.277 ± 0.348
5.332LeuGly: 5.332 ± 0.563
1.111LeuHis: 1.111 ± 0.307
3.055LeuIle: 3.055 ± 0.356
2.166LeuLys: 2.166 ± 0.385
4.832LeuLeu: 4.832 ± 0.619
1.555LeuMet: 1.555 ± 0.263
2.499LeuAsn: 2.499 ± 0.45
4.943LeuPro: 4.943 ± 0.581
2.721LeuGln: 2.721 ± 0.475
5.61LeuArg: 5.61 ± 0.66
4.888LeuSer: 4.888 ± 0.544
5.332LeuThr: 5.332 ± 0.553
5.276LeuVal: 5.276 ± 0.486
1.444LeuTrp: 1.444 ± 0.277
1.944LeuTyr: 1.944 ± 0.369
0.0LeuXaa: 0.0 ± 0.0
Met
1.777MetAla: 1.777 ± 0.313
0.222MetCys: 0.222 ± 0.146
1.055MetAsp: 1.055 ± 0.281
1.055MetGlu: 1.055 ± 0.197
0.722MetPhe: 0.722 ± 0.178
1.666MetGly: 1.666 ± 0.265
0.167MetHis: 0.167 ± 0.089
0.944MetIle: 0.944 ± 0.213
0.722MetLys: 0.722 ± 0.213
1.277MetLeu: 1.277 ± 0.212
0.555MetMet: 0.555 ± 0.212
1.0MetAsn: 1.0 ± 0.188
1.277MetPro: 1.277 ± 0.276
0.555MetGln: 0.555 ± 0.161
1.833MetArg: 1.833 ± 0.326
2.833MetSer: 2.833 ± 0.386
2.499MetThr: 2.499 ± 0.344
1.333MetVal: 1.333 ± 0.328
0.389MetTrp: 0.389 ± 0.15
0.333MetTyr: 0.333 ± 0.137
0.0MetXaa: 0.0 ± 0.0
Asn
3.277AsnAla: 3.277 ± 0.466
0.222AsnCys: 0.222 ± 0.119
1.833AsnAsp: 1.833 ± 0.262
1.999AsnGlu: 1.999 ± 0.381
0.722AsnPhe: 0.722 ± 0.218
4.888AsnGly: 4.888 ± 0.671
0.889AsnHis: 0.889 ± 0.223
1.666AsnIle: 1.666 ± 0.471
0.889AsnLys: 0.889 ± 0.24
2.333AsnLeu: 2.333 ± 0.331
0.722AsnMet: 0.722 ± 0.173
1.777AsnAsn: 1.777 ± 0.429
2.833AsnPro: 2.833 ± 0.392
0.944AsnGln: 0.944 ± 0.363
1.777AsnArg: 1.777 ± 0.349
1.277AsnSer: 1.277 ± 0.298
2.388AsnThr: 2.388 ± 0.331
2.166AsnVal: 2.166 ± 0.286
0.5AsnTrp: 0.5 ± 0.158
0.555AsnTyr: 0.555 ± 0.17
0.0AsnXaa: 0.0 ± 0.0
Pro
5.443ProAla: 5.443 ± 0.488
0.778ProCys: 0.778 ± 0.231
4.61ProAsp: 4.61 ± 0.488
4.332ProGlu: 4.332 ± 0.453
1.833ProPhe: 1.833 ± 0.371
6.165ProGly: 6.165 ± 0.672
1.722ProHis: 1.722 ± 0.344
2.388ProIle: 2.388 ± 0.316
2.333ProLys: 2.333 ± 0.418
4.166ProLeu: 4.166 ± 0.616
1.111ProMet: 1.111 ± 0.273
2.055ProAsn: 2.055 ± 0.329
3.777ProPro: 3.777 ± 0.541
2.444ProGln: 2.444 ± 0.354
3.555ProArg: 3.555 ± 0.51
3.443ProSer: 3.443 ± 0.509
3.721ProThr: 3.721 ± 0.488
4.332ProVal: 4.332 ± 0.459
1.166ProTrp: 1.166 ± 0.25
1.888ProTyr: 1.888 ± 0.362
0.0ProXaa: 0.0 ± 0.0
Gln
4.554GlnAla: 4.554 ± 0.646
0.444GlnCys: 0.444 ± 0.203
1.333GlnAsp: 1.333 ± 0.254
1.666GlnGlu: 1.666 ± 0.313
0.944GlnPhe: 0.944 ± 0.252
2.499GlnGly: 2.499 ± 0.417
0.944GlnHis: 0.944 ± 0.25
1.999GlnIle: 1.999 ± 0.431
1.222GlnLys: 1.222 ± 0.264
3.11GlnLeu: 3.11 ± 0.417
0.555GlnMet: 0.555 ± 0.195
0.833GlnAsn: 0.833 ± 0.231
2.666GlnPro: 2.666 ± 0.427
1.777GlnGln: 1.777 ± 0.453
2.111GlnArg: 2.111 ± 0.302
2.388GlnSer: 2.388 ± 0.351
1.722GlnThr: 1.722 ± 0.28
2.277GlnVal: 2.277 ± 0.372
0.889GlnTrp: 0.889 ± 0.194
0.944GlnTyr: 0.944 ± 0.286
0.0GlnXaa: 0.0 ± 0.0
Arg
6.554ArgAla: 6.554 ± 0.657
1.111ArgCys: 1.111 ± 0.307
4.11ArgAsp: 4.11 ± 0.576
4.721ArgGlu: 4.721 ± 0.591
2.277ArgPhe: 2.277 ± 0.394
3.943ArgGly: 3.943 ± 0.461
1.055ArgHis: 1.055 ± 0.29
3.388ArgIle: 3.388 ± 0.486
2.277ArgLys: 2.277 ± 0.396
4.388ArgLeu: 4.388 ± 0.499
2.388ArgMet: 2.388 ± 0.409
2.277ArgAsn: 2.277 ± 0.406
3.999ArgPro: 3.999 ± 0.444
1.999ArgGln: 1.999 ± 0.369
4.332ArgArg: 4.332 ± 0.584
4.332ArgSer: 4.332 ± 0.439
3.888ArgThr: 3.888 ± 0.549
4.832ArgVal: 4.832 ± 0.622
1.555ArgTrp: 1.555 ± 0.332
2.388ArgTyr: 2.388 ± 0.272
0.0ArgXaa: 0.0 ± 0.0
Ser
6.22SerAla: 6.22 ± 0.873
0.444SerCys: 0.444 ± 0.179
4.277SerAsp: 4.277 ± 0.498
3.388SerGlu: 3.388 ± 0.474
2.166SerPhe: 2.166 ± 0.316
7.109SerGly: 7.109 ± 0.948
1.055SerHis: 1.055 ± 0.188
2.833SerIle: 2.833 ± 0.517
2.222SerLys: 2.222 ± 0.415
3.61SerLeu: 3.61 ± 0.475
1.166SerMet: 1.166 ± 0.223
1.944SerAsn: 1.944 ± 0.449
3.666SerPro: 3.666 ± 0.451
1.611SerGln: 1.611 ± 0.299
3.166SerArg: 3.166 ± 0.377
4.554SerSer: 4.554 ± 0.791
3.832SerThr: 3.832 ± 0.599
5.11SerVal: 5.11 ± 0.699
1.444SerTrp: 1.444 ± 0.259
1.444SerTyr: 1.444 ± 0.278
0.0SerXaa: 0.0 ± 0.0
Thr
7.553ThrAla: 7.553 ± 0.757
0.778ThrCys: 0.778 ± 0.259
4.443ThrAsp: 4.443 ± 0.646
3.721ThrGlu: 3.721 ± 0.426
1.444ThrPhe: 1.444 ± 0.28
5.61ThrGly: 5.61 ± 0.632
2.222ThrHis: 2.222 ± 0.379
3.721ThrIle: 3.721 ± 0.483
2.222ThrLys: 2.222 ± 0.377
3.832ThrLeu: 3.832 ± 0.496
0.833ThrMet: 0.833 ± 0.215
2.055ThrAsn: 2.055 ± 0.394
4.943ThrPro: 4.943 ± 0.62
2.166ThrGln: 2.166 ± 0.265
4.277ThrArg: 4.277 ± 0.535
4.388ThrSer: 4.388 ± 0.527
5.498ThrThr: 5.498 ± 0.7
5.887ThrVal: 5.887 ± 0.566
1.166ThrTrp: 1.166 ± 0.198
2.055ThrTyr: 2.055 ± 0.313
0.0ThrXaa: 0.0 ± 0.0
Val
8.164ValAla: 8.164 ± 0.798
1.222ValCys: 1.222 ± 0.259
5.221ValAsp: 5.221 ± 0.538
4.11ValGlu: 4.11 ± 0.517
1.944ValPhe: 1.944 ± 0.359
5.887ValGly: 5.887 ± 0.776
1.5ValHis: 1.5 ± 0.295
3.388ValIle: 3.388 ± 0.414
2.555ValLys: 2.555 ± 0.432
6.054ValLeu: 6.054 ± 0.612
1.166ValMet: 1.166 ± 0.211
2.666ValAsn: 2.666 ± 0.416
3.777ValPro: 3.777 ± 0.492
2.333ValGln: 2.333 ± 0.314
4.388ValArg: 4.388 ± 0.616
4.054ValSer: 4.054 ± 0.553
4.721ValThr: 4.721 ± 0.401
5.998ValVal: 5.998 ± 0.647
1.944ValTrp: 1.944 ± 0.377
1.277ValTyr: 1.277 ± 0.367
0.0ValXaa: 0.0 ± 0.0
Trp
2.166TrpAla: 2.166 ± 0.312
0.278TrpCys: 0.278 ± 0.127
1.222TrpAsp: 1.222 ± 0.262
1.166TrpGlu: 1.166 ± 0.269
0.611TrpPhe: 0.611 ± 0.174
1.166TrpGly: 1.166 ± 0.239
0.611TrpHis: 0.611 ± 0.217
1.166TrpIle: 1.166 ± 0.243
0.778TrpLys: 0.778 ± 0.2
1.666TrpLeu: 1.666 ± 0.323
0.944TrpMet: 0.944 ± 0.213
0.444TrpAsn: 0.444 ± 0.204
1.277TrpPro: 1.277 ± 0.301
1.111TrpGln: 1.111 ± 0.265
2.166TrpArg: 2.166 ± 0.312
1.666TrpSer: 1.666 ± 0.359
1.166TrpThr: 1.166 ± 0.299
1.555TrpVal: 1.555 ± 0.47
0.722TrpTrp: 0.722 ± 0.212
0.5TrpTyr: 0.5 ± 0.169
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.888TyrAla: 2.888 ± 0.447
0.222TyrCys: 0.222 ± 0.135
2.111TyrAsp: 2.111 ± 0.428
1.666TyrGlu: 1.666 ± 0.33
0.944TyrPhe: 0.944 ± 0.24
2.111TyrGly: 2.111 ± 0.38
0.389TyrHis: 0.389 ± 0.14
0.833TyrIle: 0.833 ± 0.206
0.666TyrLys: 0.666 ± 0.211
2.166TyrLeu: 2.166 ± 0.348
0.333TyrMet: 0.333 ± 0.121
0.889TyrAsn: 0.889 ± 0.296
1.389TyrPro: 1.389 ± 0.297
0.944TyrGln: 0.944 ± 0.227
1.611TyrArg: 1.611 ± 0.302
1.055TyrSer: 1.055 ± 0.244
1.666TyrThr: 1.666 ± 0.341
2.444TyrVal: 2.444 ± 0.318
0.722TyrTrp: 0.722 ± 0.192
0.666TyrTyr: 0.666 ± 0.248
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 103 proteins (18006 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski