Microbacterium phage Rasputia

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rogerhendrixvirus; unclassified Rogerhendrixvirus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LFA6|A0A7G8LFA6_9CAUD Uncharacterized protein OS=Microbacterium phage Rasputia OX=2762419 GN=38 PE=4 SV=1
MM1 pKa = 7.82TYY3 pKa = 10.41RR4 pKa = 11.84DD5 pKa = 4.63GPCPEE10 pKa = 4.63NPPLHH15 pKa = 6.37WNCDD19 pKa = 2.75RR20 pKa = 11.84CTPCNEE26 pKa = 4.31GLHH29 pKa = 5.26EE30 pKa = 4.35ACRR33 pKa = 11.84GWVYY37 pKa = 9.98WRR39 pKa = 11.84CEE41 pKa = 4.39CYY43 pKa = 10.61CQDD46 pKa = 4.5LDD48 pKa = 5.37EE49 pKa = 5.17EE50 pKa = 4.78DD51 pKa = 4.18SEE53 pKa = 4.9QGLDD57 pKa = 3.44HH58 pKa = 8.04DD59 pKa = 4.92EE60 pKa = 4.25GG61 pKa = 4.62

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LF78|A0A7G8LF78_9CAUD DNA ligase OS=Microbacterium phage Rasputia OX=2762419 GN=10 PE=4 SV=1
MM1 pKa = 7.95AEE3 pKa = 4.16TLEE6 pKa = 4.25DD7 pKa = 3.69LQRR10 pKa = 11.84QLKK13 pKa = 8.6QAEE16 pKa = 4.16RR17 pKa = 11.84DD18 pKa = 3.69EE19 pKa = 5.38VIDD22 pKa = 3.23GHH24 pKa = 7.3RR25 pKa = 11.84SDD27 pKa = 3.5RR28 pKa = 11.84AYY30 pKa = 10.72ARR32 pKa = 11.84RR33 pKa = 11.84QTRR36 pKa = 11.84RR37 pKa = 11.84GQFFAQEE44 pKa = 3.88SRR46 pKa = 11.84RR47 pKa = 11.84DD48 pKa = 3.71FLAQTILKK56 pKa = 10.04RR57 pKa = 11.84RR58 pKa = 11.84IEE60 pKa = 4.19RR61 pKa = 11.84LTQGAA66 pKa = 3.75

Molecular weight: 7.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

153

0

153

30873

32

1282

201.8

22.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.631 ± 0.374

0.784 ± 0.101

6.449 ± 0.232

7.256 ± 0.351

2.753 ± 0.121

7.806 ± 0.19

1.989 ± 0.128

4.736 ± 0.198

3.935 ± 0.216

7.9 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.124

2.983 ± 0.146

5.581 ± 0.334

3.498 ± 0.094

6.695 ± 0.3

5.834 ± 0.268

6.611 ± 0.312

7.249 ± 0.196

2.17 ± 0.132

2.449 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski