Amino acid dipepetide frequency for Microbacterium phage Rasputia

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.378AlaAla: 13.378 ± 1.142
0.453AlaCys: 0.453 ± 0.115
5.895AlaAsp: 5.895 ± 0.496
8.875AlaGlu: 8.875 ± 0.638
3.207AlaPhe: 3.207 ± 0.362
7.353AlaGly: 7.353 ± 0.596
2.17AlaHis: 2.17 ± 0.282
5.28AlaIle: 5.28 ± 0.462
3.984AlaLys: 3.984 ± 0.425
9.556AlaLeu: 9.556 ± 0.515
2.883AlaMet: 2.883 ± 0.321
2.365AlaAsn: 2.365 ± 0.318
5.377AlaPro: 5.377 ± 0.433
4.47AlaGln: 4.47 ± 0.489
6.576AlaArg: 6.576 ± 0.465
6.284AlaSer: 6.284 ± 0.509
5.928AlaThr: 5.928 ± 0.588
7.742AlaVal: 7.742 ± 0.975
2.17AlaTrp: 2.17 ± 0.335
2.365AlaTyr: 2.365 ± 0.285
0.0AlaXaa: 0.0 ± 0.0
Cys
0.68CysAla: 0.68 ± 0.173
0.032CysCys: 0.032 ± 0.031
0.745CysAsp: 0.745 ± 0.201
0.389CysGlu: 0.389 ± 0.12
0.227CysPhe: 0.227 ± 0.087
1.231CysGly: 1.231 ± 0.242
0.227CysHis: 0.227 ± 0.105
0.259CysIle: 0.259 ± 0.094
0.162CysLys: 0.162 ± 0.065
0.356CysLeu: 0.356 ± 0.095
0.097CysMet: 0.097 ± 0.049
0.162CysAsn: 0.162 ± 0.072
0.907CysPro: 0.907 ± 0.179
0.227CysGln: 0.227 ± 0.105
0.356CysArg: 0.356 ± 0.123
0.453CysSer: 0.453 ± 0.138
0.453CysThr: 0.453 ± 0.136
0.518CysVal: 0.518 ± 0.143
0.162CysTrp: 0.162 ± 0.071
0.194CysTyr: 0.194 ± 0.074
0.0CysXaa: 0.0 ± 0.0
Asp
6.252AspAla: 6.252 ± 0.584
0.518AspCys: 0.518 ± 0.13
4.697AspAsp: 4.697 ± 0.46
5.312AspGlu: 5.312 ± 0.53
1.976AspPhe: 1.976 ± 0.251
5.442AspGly: 5.442 ± 0.432
1.36AspHis: 1.36 ± 0.221
2.656AspIle: 2.656 ± 0.336
1.782AspLys: 1.782 ± 0.206
5.96AspLeu: 5.96 ± 0.488
1.166AspMet: 1.166 ± 0.163
1.36AspAsn: 1.36 ± 0.223
4.438AspPro: 4.438 ± 0.405
2.105AspGln: 2.105 ± 0.287
4.341AspArg: 4.341 ± 0.357
3.693AspSer: 3.693 ± 0.343
3.498AspThr: 3.498 ± 0.343
4.243AspVal: 4.243 ± 0.343
1.393AspTrp: 1.393 ± 0.191
2.3AspTyr: 2.3 ± 0.403
0.0AspXaa: 0.0 ± 0.0
Glu
7.547GluAla: 7.547 ± 0.573
0.486GluCys: 0.486 ± 0.14
5.571GluAsp: 5.571 ± 0.497
5.733GluGlu: 5.733 ± 0.533
2.008GluPhe: 2.008 ± 0.29
4.729GluGly: 4.729 ± 0.468
1.846GluHis: 1.846 ± 0.251
2.915GluIle: 2.915 ± 0.428
3.239GluLys: 3.239 ± 0.357
6.608GluLeu: 6.608 ± 0.469
1.976GluMet: 1.976 ± 0.24
2.105GluAsn: 2.105 ± 0.243
3.401GluPro: 3.401 ± 0.478
3.142GluGln: 3.142 ± 0.356
5.183GluArg: 5.183 ± 0.486
3.757GluSer: 3.757 ± 0.382
3.855GluThr: 3.855 ± 0.438
5.215GluVal: 5.215 ± 0.456
1.101GluTrp: 1.101 ± 0.181
2.138GluTyr: 2.138 ± 0.272
0.0GluXaa: 0.0 ± 0.0
Phe
2.397PheAla: 2.397 ± 0.271
0.194PheCys: 0.194 ± 0.072
2.073PheAsp: 2.073 ± 0.282
1.717PheGlu: 1.717 ± 0.215
0.842PhePhe: 0.842 ± 0.212
2.883PheGly: 2.883 ± 0.33
0.615PheHis: 0.615 ± 0.17
1.555PheIle: 1.555 ± 0.317
0.907PheLys: 0.907 ± 0.209
2.332PheLeu: 2.332 ± 0.292
0.486PheMet: 0.486 ± 0.129
0.81PheAsn: 0.81 ± 0.187
1.328PhePro: 1.328 ± 0.209
1.037PheGln: 1.037 ± 0.174
1.522PheArg: 1.522 ± 0.251
1.652PheSer: 1.652 ± 0.255
2.332PheThr: 2.332 ± 0.249
1.749PheVal: 1.749 ± 0.247
0.745PheTrp: 0.745 ± 0.174
0.356PheTyr: 0.356 ± 0.08
0.0PheXaa: 0.0 ± 0.0
Gly
6.997GlyAla: 6.997 ± 0.597
0.615GlyCys: 0.615 ± 0.198
3.887GlyAsp: 3.887 ± 0.272
5.183GlyGlu: 5.183 ± 0.412
2.203GlyPhe: 2.203 ± 0.263
6.77GlyGly: 6.77 ± 0.567
1.749GlyHis: 1.749 ± 0.277
3.239GlyIle: 3.239 ± 0.401
3.498GlyLys: 3.498 ± 0.396
6.673GlyLeu: 6.673 ± 0.521
2.397GlyMet: 2.397 ± 0.303
2.527GlyAsn: 2.527 ± 0.275
3.369GlyPro: 3.369 ± 0.305
3.239GlyGln: 3.239 ± 0.343
4.6GlyArg: 4.6 ± 0.473
5.021GlySer: 5.021 ± 0.479
5.345GlyThr: 5.345 ± 0.805
6.284GlyVal: 6.284 ± 0.492
2.105GlyTrp: 2.105 ± 0.289
2.365GlyTyr: 2.365 ± 0.229
0.0GlyXaa: 0.0 ± 0.0
His
2.073HisAla: 2.073 ± 0.268
0.162HisCys: 0.162 ± 0.073
1.393HisAsp: 1.393 ± 0.212
1.166HisGlu: 1.166 ± 0.194
0.583HisPhe: 0.583 ± 0.148
1.425HisGly: 1.425 ± 0.2
0.324HisHis: 0.324 ± 0.112
1.134HisIle: 1.134 ± 0.162
0.421HisLys: 0.421 ± 0.12
1.814HisLeu: 1.814 ± 0.25
0.518HisMet: 0.518 ± 0.142
0.453HisAsn: 0.453 ± 0.119
1.134HisPro: 1.134 ± 0.191
0.648HisGln: 0.648 ± 0.139
1.587HisArg: 1.587 ± 0.199
1.198HisSer: 1.198 ± 0.194
1.263HisThr: 1.263 ± 0.21
1.393HisVal: 1.393 ± 0.224
0.453HisTrp: 0.453 ± 0.117
0.745HisTyr: 0.745 ± 0.146
0.0HisXaa: 0.0 ± 0.0
Ile
4.924IleAla: 4.924 ± 0.458
0.356IleCys: 0.356 ± 0.108
4.632IleAsp: 4.632 ± 0.387
4.081IleGlu: 4.081 ± 0.364
1.166IlePhe: 1.166 ± 0.203
3.855IleGly: 3.855 ± 0.429
0.907IleHis: 0.907 ± 0.151
1.911IleIle: 1.911 ± 0.329
1.263IleLys: 1.263 ± 0.212
2.365IleLeu: 2.365 ± 0.356
1.101IleMet: 1.101 ± 0.23
1.134IleAsn: 1.134 ± 0.189
2.948IlePro: 2.948 ± 0.405
1.717IleGln: 1.717 ± 0.244
3.174IleArg: 3.174 ± 0.298
2.624IleSer: 2.624 ± 0.352
3.401IleThr: 3.401 ± 0.303
4.114IleVal: 4.114 ± 0.373
0.81IleTrp: 0.81 ± 0.166
0.875IleTyr: 0.875 ± 0.176
0.0IleXaa: 0.0 ± 0.0
Lys
4.794LysAla: 4.794 ± 0.373
0.356LysCys: 0.356 ± 0.116
2.267LysAsp: 2.267 ± 0.25
2.332LysGlu: 2.332 ± 0.284
0.939LysPhe: 0.939 ± 0.168
2.105LysGly: 2.105 ± 0.327
1.134LysHis: 1.134 ± 0.188
2.494LysIle: 2.494 ± 0.312
2.203LysLys: 2.203 ± 0.26
3.757LysLeu: 3.757 ± 0.358
0.875LysMet: 0.875 ± 0.16
1.198LysAsn: 1.198 ± 0.209
1.911LysPro: 1.911 ± 0.308
1.263LysGln: 1.263 ± 0.155
3.11LysArg: 3.11 ± 0.345
2.332LysSer: 2.332 ± 0.326
2.3LysThr: 2.3 ± 0.271
2.527LysVal: 2.527 ± 0.286
0.648LysTrp: 0.648 ± 0.144
0.81LysTyr: 0.81 ± 0.142
0.0LysXaa: 0.0 ± 0.0
Leu
8.066LeuAla: 8.066 ± 0.679
0.648LeuCys: 0.648 ± 0.145
5.507LeuAsp: 5.507 ± 0.569
6.154LeuGlu: 6.154 ± 0.432
1.652LeuPhe: 1.652 ± 0.203
5.766LeuGly: 5.766 ± 0.443
1.62LeuHis: 1.62 ± 0.247
4.179LeuIle: 4.179 ± 0.462
3.207LeuLys: 3.207 ± 0.323
5.377LeuLeu: 5.377 ± 0.474
2.721LeuMet: 2.721 ± 0.312
2.332LeuAsn: 2.332 ± 0.252
4.47LeuPro: 4.47 ± 0.373
2.527LeuGln: 2.527 ± 0.278
5.15LeuArg: 5.15 ± 0.492
5.701LeuSer: 5.701 ± 0.519
5.409LeuThr: 5.409 ± 0.435
5.312LeuVal: 5.312 ± 0.476
1.587LeuTrp: 1.587 ± 0.212
1.62LeuTyr: 1.62 ± 0.256
0.0LeuXaa: 0.0 ± 0.0
Met
2.883MetAla: 2.883 ± 0.349
0.065MetCys: 0.065 ± 0.048
1.263MetAsp: 1.263 ± 0.18
1.425MetGlu: 1.425 ± 0.158
0.583MetPhe: 0.583 ± 0.156
1.62MetGly: 1.62 ± 0.258
0.68MetHis: 0.68 ± 0.148
1.49MetIle: 1.49 ± 0.232
1.36MetLys: 1.36 ± 0.206
1.62MetLeu: 1.62 ± 0.206
0.68MetMet: 0.68 ± 0.152
0.907MetAsn: 0.907 ± 0.153
1.879MetPro: 1.879 ± 0.263
0.777MetGln: 0.777 ± 0.155
1.782MetArg: 1.782 ± 0.274
2.656MetSer: 2.656 ± 0.318
2.689MetThr: 2.689 ± 0.279
1.62MetVal: 1.62 ± 0.249
0.324MetTrp: 0.324 ± 0.091
0.615MetTyr: 0.615 ± 0.122
0.0MetXaa: 0.0 ± 0.0
Asn
3.466AsnAla: 3.466 ± 0.323
0.389AsnCys: 0.389 ± 0.126
1.717AsnAsp: 1.717 ± 0.277
1.976AsnGlu: 1.976 ± 0.253
0.583AsnPhe: 0.583 ± 0.155
3.434AsnGly: 3.434 ± 0.366
0.421AsnHis: 0.421 ± 0.13
1.069AsnIle: 1.069 ± 0.209
0.745AsnLys: 0.745 ± 0.177
2.041AsnLeu: 2.041 ± 0.252
0.648AsnMet: 0.648 ± 0.151
0.842AsnAsn: 0.842 ± 0.19
2.689AsnPro: 2.689 ± 0.599
0.777AsnGln: 0.777 ± 0.132
1.717AsnArg: 1.717 ± 0.275
1.749AsnSer: 1.749 ± 0.278
1.911AsnThr: 1.911 ± 0.259
2.267AsnVal: 2.267 ± 0.368
0.907AsnTrp: 0.907 ± 0.17
0.486AsnTyr: 0.486 ± 0.128
0.0AsnXaa: 0.0 ± 0.0
Pro
7.029ProAla: 7.029 ± 1.46
0.453ProCys: 0.453 ± 0.141
4.017ProAsp: 4.017 ± 0.491
4.697ProGlu: 4.697 ± 0.603
1.717ProPhe: 1.717 ± 0.27
4.341ProGly: 4.341 ± 0.715
1.069ProHis: 1.069 ± 0.214
2.883ProIle: 2.883 ± 0.313
1.976ProLys: 1.976 ± 0.305
3.401ProLeu: 3.401 ± 0.356
1.296ProMet: 1.296 ± 0.22
1.652ProAsn: 1.652 ± 0.424
2.721ProPro: 2.721 ± 0.347
1.393ProGln: 1.393 ± 0.179
3.466ProArg: 3.466 ± 0.362
3.142ProSer: 3.142 ± 0.387
4.276ProThr: 4.276 ± 0.574
3.757ProVal: 3.757 ± 0.38
1.134ProTrp: 1.134 ± 0.188
1.393ProTyr: 1.393 ± 0.245
0.0ProXaa: 0.0 ± 0.0
Gln
3.693GlnAla: 3.693 ± 0.323
0.389GlnCys: 0.389 ± 0.116
1.62GlnAsp: 1.62 ± 0.185
2.3GlnGlu: 2.3 ± 0.328
0.907GlnPhe: 0.907 ± 0.168
2.656GlnGly: 2.656 ± 0.314
0.81GlnHis: 0.81 ± 0.141
1.814GlnIle: 1.814 ± 0.223
1.976GlnLys: 1.976 ± 0.274
2.786GlnLeu: 2.786 ± 0.336
1.004GlnMet: 1.004 ± 0.195
1.458GlnAsn: 1.458 ± 0.203
1.782GlnPro: 1.782 ± 0.252
1.36GlnGln: 1.36 ± 0.251
2.656GlnArg: 2.656 ± 0.349
1.782GlnSer: 1.782 ± 0.288
1.911GlnThr: 1.911 ± 0.258
2.527GlnVal: 2.527 ± 0.264
0.777GlnTrp: 0.777 ± 0.155
0.777GlnTyr: 0.777 ± 0.157
0.0GlnXaa: 0.0 ± 0.0
Arg
7.385ArgAla: 7.385 ± 0.446
0.648ArgCys: 0.648 ± 0.158
4.179ArgAsp: 4.179 ± 0.413
4.956ArgGlu: 4.956 ± 0.497
1.846ArgPhe: 1.846 ± 0.269
4.988ArgGly: 4.988 ± 0.524
1.101ArgHis: 1.101 ± 0.209
3.11ArgIle: 3.11 ± 0.34
2.915ArgLys: 2.915 ± 0.315
5.118ArgLeu: 5.118 ± 0.373
1.879ArgMet: 1.879 ± 0.252
2.073ArgAsn: 2.073 ± 0.254
3.466ArgPro: 3.466 ± 0.328
2.203ArgGln: 2.203 ± 0.289
5.798ArgArg: 5.798 ± 0.653
3.174ArgSer: 3.174 ± 0.363
3.952ArgThr: 3.952 ± 0.327
4.405ArgVal: 4.405 ± 0.373
1.944ArgTrp: 1.944 ± 0.283
1.814ArgTyr: 1.814 ± 0.268
0.0ArgXaa: 0.0 ± 0.0
Ser
5.928SerAla: 5.928 ± 0.512
0.356SerCys: 0.356 ± 0.122
3.693SerAsp: 3.693 ± 0.338
4.081SerGlu: 4.081 ± 0.383
1.944SerPhe: 1.944 ± 0.211
4.664SerGly: 4.664 ± 0.472
0.745SerHis: 0.745 ± 0.196
2.689SerIle: 2.689 ± 0.337
2.008SerLys: 2.008 ± 0.263
5.15SerLeu: 5.15 ± 0.451
2.365SerMet: 2.365 ± 0.266
1.684SerAsn: 1.684 ± 0.196
2.721SerPro: 2.721 ± 0.461
1.49SerGln: 1.49 ± 0.211
3.725SerArg: 3.725 ± 0.374
3.272SerSer: 3.272 ± 0.383
4.276SerThr: 4.276 ± 0.598
4.762SerVal: 4.762 ± 0.4
1.328SerTrp: 1.328 ± 0.227
1.425SerTyr: 1.425 ± 0.217
0.0SerXaa: 0.0 ± 0.0
Thr
6.964ThrAla: 6.964 ± 0.614
0.745ThrCys: 0.745 ± 0.234
3.919ThrAsp: 3.919 ± 0.42
4.502ThrGlu: 4.502 ± 0.428
1.62ThrPhe: 1.62 ± 0.264
5.28ThrGly: 5.28 ± 0.572
1.134ThrHis: 1.134 ± 0.168
3.239ThrIle: 3.239 ± 0.474
2.753ThrLys: 2.753 ± 0.335
5.021ThrLeu: 5.021 ± 0.428
1.652ThrMet: 1.652 ± 0.221
1.944ThrAsn: 1.944 ± 0.237
4.891ThrPro: 4.891 ± 1.096
2.235ThrGln: 2.235 ± 0.262
3.725ThrArg: 3.725 ± 0.412
3.628ThrSer: 3.628 ± 0.569
4.308ThrThr: 4.308 ± 0.445
5.312ThrVal: 5.312 ± 0.422
1.393ThrTrp: 1.393 ± 0.249
1.846ThrTyr: 1.846 ± 0.27
0.0ThrXaa: 0.0 ± 0.0
Val
7.321ValAla: 7.321 ± 0.717
0.615ValCys: 0.615 ± 0.172
4.891ValAsp: 4.891 ± 0.392
5.021ValGlu: 5.021 ± 0.423
2.041ValPhe: 2.041 ± 0.253
5.377ValGly: 5.377 ± 0.511
1.263ValHis: 1.263 ± 0.206
3.757ValIle: 3.757 ± 0.406
3.11ValLys: 3.11 ± 0.353
5.312ValLeu: 5.312 ± 0.509
1.911ValMet: 1.911 ± 0.257
2.915ValAsn: 2.915 ± 0.346
4.6ValPro: 4.6 ± 1.261
2.786ValGln: 2.786 ± 0.279
4.47ValArg: 4.47 ± 0.388
3.855ValSer: 3.855 ± 0.357
5.831ValThr: 5.831 ± 0.487
4.6ValVal: 4.6 ± 0.373
1.425ValTrp: 1.425 ± 0.247
1.393ValTyr: 1.393 ± 0.225
0.0ValXaa: 0.0 ± 0.0
Trp
2.073TrpAla: 2.073 ± 0.26
0.259TrpCys: 0.259 ± 0.095
1.101TrpAsp: 1.101 ± 0.212
1.198TrpGlu: 1.198 ± 0.199
0.842TrpPhe: 0.842 ± 0.142
1.62TrpGly: 1.62 ± 0.24
0.453TrpHis: 0.453 ± 0.107
0.842TrpIle: 0.842 ± 0.18
1.134TrpLys: 1.134 ± 0.198
1.684TrpLeu: 1.684 ± 0.215
0.615TrpMet: 0.615 ± 0.149
1.134TrpAsn: 1.134 ± 0.424
0.68TrpPro: 0.68 ± 0.148
0.745TrpGln: 0.745 ± 0.131
1.749TrpArg: 1.749 ± 0.309
1.037TrpSer: 1.037 ± 0.148
1.49TrpThr: 1.49 ± 0.245
2.041TrpVal: 2.041 ± 0.289
0.648TrpTrp: 0.648 ± 0.152
0.356TrpTyr: 0.356 ± 0.089
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.462TyrAla: 2.462 ± 0.3
0.097TyrCys: 0.097 ± 0.055
1.425TyrAsp: 1.425 ± 0.249
1.458TyrGlu: 1.458 ± 0.229
0.648TyrPhe: 0.648 ± 0.14
2.527TyrGly: 2.527 ± 0.283
0.259TyrHis: 0.259 ± 0.099
0.842TyrIle: 0.842 ± 0.127
0.713TyrLys: 0.713 ± 0.16
2.073TyrLeu: 2.073 ± 0.255
0.615TyrMet: 0.615 ± 0.133
0.777TyrAsn: 0.777 ± 0.173
1.101TyrPro: 1.101 ± 0.179
0.842TyrGln: 0.842 ± 0.153
2.267TyrArg: 2.267 ± 0.289
1.328TyrSer: 1.328 ± 0.209
1.684TyrThr: 1.684 ± 0.29
2.105TyrVal: 2.105 ± 0.621
0.648TyrTrp: 0.648 ± 0.21
0.615TyrTyr: 0.615 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 153 proteins (30873 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski