Treponema succinifaciens (strain ATCC 33096 / DSM 2489 / 6091)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Treponemataceae; Treponema; Treponema succinifaciens

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2561 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2NS82|F2NS82_TRES6 Chaperone protein ClpB OS=Treponema succinifaciens (strain ATCC 33096 / DSM 2489 / 6091) OX=869209 GN=clpB PE=3 SV=1
MM1 pKa = 6.99QFFWGGALISCSNGSSSSDD20 pKa = 3.35EE21 pKa = 5.0DD22 pKa = 4.58DD23 pKa = 5.47DD24 pKa = 4.39EE25 pKa = 6.04SSYY28 pKa = 9.57GTKK31 pKa = 9.53IYY33 pKa = 10.64SEE35 pKa = 4.42SVTFSEE41 pKa = 4.95TPTQIVSAEE50 pKa = 4.08TLADD54 pKa = 4.04LSIKK58 pKa = 10.62SLTIKK63 pKa = 10.23VINVNYY69 pKa = 10.27SGSDD73 pKa = 3.37DD74 pKa = 3.14WWFTACSDD82 pKa = 4.1SSWSNEE88 pKa = 4.29AKK90 pKa = 10.82LEE92 pKa = 4.09WQSGEE97 pKa = 4.17GTNSLYY103 pKa = 10.95LATITDD109 pKa = 3.9AAAIAAYY116 pKa = 8.61KK117 pKa = 10.86ANGIYY122 pKa = 10.03IAGTDD127 pKa = 3.41SATAAVSVFYY137 pKa = 11.39VEE139 pKa = 5.73GEE141 pKa = 4.12AEE143 pKa = 4.04EE144 pKa = 5.02SNASSGSDD152 pKa = 2.99SQTEE156 pKa = 4.01NTTTSNEE163 pKa = 4.18TSSSEE168 pKa = 4.05NEE170 pKa = 4.13TTGTSSNEE178 pKa = 3.79NEE180 pKa = 4.54DD181 pKa = 3.73NGSSSEE187 pKa = 3.98EE188 pKa = 3.61ALIEE192 pKa = 4.28SFSSNTRR199 pKa = 11.84SANFTISSSYY209 pKa = 9.38QLLMKK214 pKa = 10.8YY215 pKa = 7.55EE216 pKa = 4.45TVNSASKK223 pKa = 9.12VTITISDD230 pKa = 3.87VQTGTSNGDD239 pKa = 2.08WWFASGTDD247 pKa = 3.58SSKK250 pKa = 10.44WKK252 pKa = 10.66ADD254 pKa = 3.51IAWKK258 pKa = 9.87EE259 pKa = 3.75GNYY262 pKa = 9.98YY263 pKa = 9.99EE264 pKa = 5.95LEE266 pKa = 3.98ISDD269 pKa = 3.07ISEE272 pKa = 4.1YY273 pKa = 11.27SKK275 pKa = 11.15GIYY278 pKa = 10.02LGGLEE283 pKa = 4.37GLSGTVTVSYY293 pKa = 10.32EE294 pKa = 3.68

Molecular weight: 31.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2NSN6|F2NSN6_TRES6 Uncharacterized protein OS=Treponema succinifaciens (strain ATCC 33096 / DSM 2489 / 6091) OX=869209 GN=Tresu_1927 PE=4 SV=1
MM1 pKa = 7.67VLISVFLYY9 pKa = 8.43TVRR12 pKa = 11.84FPYY15 pKa = 10.48FKK17 pKa = 10.57RR18 pKa = 11.84IDD20 pKa = 3.41FRR22 pKa = 11.84TRR24 pKa = 11.84QVRR27 pKa = 11.84FSALRR32 pKa = 11.84RR33 pKa = 11.84TITGFLEE40 pKa = 3.87VGVWNRR46 pKa = 11.84FIGFRR51 pKa = 11.84VNAVGFSTALFLDD64 pKa = 4.15FHH66 pKa = 6.65QPRR69 pKa = 11.84ILQLPQGVDD78 pKa = 2.87RR79 pKa = 11.84FLPPAAEE86 pKa = 4.21QSDD89 pKa = 3.86HH90 pKa = 6.77LADD93 pKa = 3.77GVVQVDD99 pKa = 3.8PPVLVRR105 pKa = 11.84PAVLPGQLRR114 pKa = 11.84PAQDD118 pKa = 3.12KK119 pKa = 10.94GIQHH123 pKa = 6.71LRR125 pKa = 11.84FVAQGLEE132 pKa = 4.13CGCFKK137 pKa = 11.06KK138 pKa = 10.75EE139 pKa = 3.51IGEE142 pKa = 4.28PGEE145 pKa = 4.11TEE147 pKa = 3.68RR148 pKa = 11.84LFRR151 pKa = 11.84LVNINRR157 pKa = 11.84VCHH160 pKa = 6.16SVFCGRR166 pKa = 11.84LEE168 pKa = 3.77ARR170 pKa = 11.84FPGRR174 pKa = 11.84MINDD178 pKa = 3.75TSRR181 pKa = 11.84PGG183 pKa = 3.06

Molecular weight: 20.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2561

0

2561

829679

30

2901

324.0

36.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.95 ± 0.043

1.49 ± 0.02

5.367 ± 0.041

7.477 ± 0.055

5.371 ± 0.046

6.199 ± 0.046

1.44 ± 0.017

7.66 ± 0.039

8.436 ± 0.046

8.72 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.257 ± 0.02

5.396 ± 0.04

3.322 ± 0.028

2.991 ± 0.024

3.765 ± 0.036

7.441 ± 0.048

4.958 ± 0.036

6.111 ± 0.045

0.954 ± 0.016

3.692 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski