Periconia macrospinosa

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Massarineae; Periconiaceae; Periconia

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18722 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V1E641|A0A2V1E641_9PLEO V-type proton ATPase subunit a OS=Periconia macrospinosa OX=97972 GN=DM02DRAFT_725363 PE=3 SV=1
MM1 pKa = 7.0TTTAWATPGFEE12 pKa = 5.19LNPLSDD18 pKa = 3.56VMDD21 pKa = 3.47IHH23 pKa = 7.61SDD25 pKa = 3.25RR26 pKa = 11.84GVDD29 pKa = 3.52VDD31 pKa = 5.08NVDD34 pKa = 2.94VDD36 pKa = 3.56IDD38 pKa = 4.19FDD40 pKa = 4.26PSRR43 pKa = 11.84SPATYY48 pKa = 10.91GDD50 pKa = 4.13DD51 pKa = 5.17LSLKK55 pKa = 10.44DD56 pKa = 4.06AVLEE60 pKa = 4.29GEE62 pKa = 5.12PGPQTASADD71 pKa = 3.49QDD73 pKa = 3.72DD74 pKa = 4.31FMVDD78 pKa = 3.28NEE80 pKa = 4.55DD81 pKa = 4.97LIEE84 pKa = 4.61EE85 pKa = 4.05DD86 pKa = 3.58TIAYY90 pKa = 9.82EE91 pKa = 3.98EE92 pKa = 4.6DD93 pKa = 3.28AVIVDD98 pKa = 4.41EE99 pKa = 5.07PSANQNEE106 pKa = 4.39VDD108 pKa = 4.29HH109 pKa = 7.1SVDD112 pKa = 4.86DD113 pKa = 4.69DD114 pKa = 5.1LIDD117 pKa = 4.0YY118 pKa = 11.06SDD120 pKa = 5.09DD121 pKa = 4.25EE122 pKa = 4.59EE123 pKa = 6.69DD124 pKa = 3.67STPKK128 pKa = 10.52EE129 pKa = 4.03DD130 pKa = 5.09GISKK134 pKa = 8.5NTDD137 pKa = 2.69LTSARR142 pKa = 11.84NDD144 pKa = 3.3TAASVAEE151 pKa = 4.3SGPTEE156 pKa = 4.07IGPTSTEE163 pKa = 3.9IEE165 pKa = 4.27HH166 pKa = 6.15TAVDD170 pKa = 3.74PADD173 pKa = 4.34EE174 pKa = 4.23YY175 pKa = 11.72DD176 pKa = 3.41EE177 pKa = 5.17SKK179 pKa = 10.38IDD181 pKa = 3.65EE182 pKa = 4.88LEE184 pKa = 4.29TTGEE188 pKa = 4.13PGEE191 pKa = 4.22NWQEE195 pKa = 3.9AQEE198 pKa = 4.32AAHH201 pKa = 5.46QQEE204 pKa = 4.17LSIYY208 pKa = 10.76ADD210 pKa = 3.25VDD212 pKa = 4.3SNDD215 pKa = 3.6AQPEE219 pKa = 4.13EE220 pKa = 4.21NEE222 pKa = 4.31YY223 pKa = 11.31DD224 pKa = 4.15HH225 pKa = 7.39EE226 pKa = 4.51DD227 pKa = 3.75QGAQKK232 pKa = 10.26QDD234 pKa = 2.94NSEE237 pKa = 3.98DD238 pKa = 3.66VEE240 pKa = 4.78YY241 pKa = 10.81VAHH244 pKa = 6.98ASNDD248 pKa = 2.81IHH250 pKa = 7.55SPSGQEE256 pKa = 3.84IEE258 pKa = 4.29LHH260 pKa = 5.92PVTINYY266 pKa = 10.0SGAEE270 pKa = 3.58LWLFKK275 pKa = 10.79HH276 pKa = 6.55HH277 pKa = 7.5DD278 pKa = 3.88YY279 pKa = 11.27EE280 pKa = 6.28DD281 pKa = 3.68SGDD284 pKa = 3.92YY285 pKa = 10.64LVEE288 pKa = 4.37DD289 pKa = 3.85TSITNKK295 pKa = 9.97PISSVLEE302 pKa = 3.86ACRR305 pKa = 11.84SALGEE310 pKa = 4.46DD311 pKa = 3.4VTDD314 pKa = 5.09DD315 pKa = 3.43IEE317 pKa = 5.6LGFRR321 pKa = 11.84LDD323 pKa = 3.26NFRR326 pKa = 11.84NIEE329 pKa = 4.13LYY331 pKa = 10.09QDD333 pKa = 3.54HH334 pKa = 6.98SSCAFITLEE343 pKa = 4.64HH344 pKa = 6.42IVGLYY349 pKa = 9.85LQLHH353 pKa = 5.62AQDD356 pKa = 5.29GISDD360 pKa = 4.01PEE362 pKa = 4.21SFYY365 pKa = 9.02MTLLSRR371 pKa = 11.84PRR373 pKa = 11.84VSALFNALNEE383 pKa = 4.24AASEE387 pKa = 4.65GIGHH391 pKa = 6.48VGLEE395 pKa = 3.94KK396 pKa = 10.63AIAAGLTSFNARR408 pKa = 11.84LSHH411 pKa = 5.07NTAVNSYY418 pKa = 10.09DD419 pKa = 3.01WGNEE423 pKa = 3.78EE424 pKa = 3.97EE425 pKa = 4.59HH426 pKa = 6.27QGHH429 pKa = 5.87VADD432 pKa = 4.25VANGNQVEE440 pKa = 4.52GNGNGEE446 pKa = 4.18HH447 pKa = 5.76EE448 pKa = 4.45THH450 pKa = 6.47NEE452 pKa = 3.85HH453 pKa = 7.08EE454 pKa = 4.38EE455 pKa = 3.99EE456 pKa = 4.03EE457 pKa = 4.36QYY459 pKa = 11.44EE460 pKa = 4.39NSAQTHH466 pKa = 5.71EE467 pKa = 4.84GGAQEE472 pKa = 4.34SEE474 pKa = 4.14LVEE477 pKa = 4.66EE478 pKa = 5.05IIEE481 pKa = 4.41PTTQDD486 pKa = 3.21QNSFATDD493 pKa = 3.51GALSEE498 pKa = 4.63PEE500 pKa = 4.1NVTLNVADD508 pKa = 4.62NDD510 pKa = 4.7DD511 pKa = 4.1IQPAPATGSPRR522 pKa = 11.84TEE524 pKa = 3.94EE525 pKa = 3.72QAGDD529 pKa = 3.55NDD531 pKa = 4.12VQKK534 pKa = 11.06SPSQQEE540 pKa = 3.78DD541 pKa = 3.67DD542 pKa = 5.07DD543 pKa = 4.51FVDD546 pKa = 3.85YY547 pKa = 11.39SDD549 pKa = 6.2DD550 pKa = 3.74EE551 pKa = 5.6DD552 pKa = 3.84EE553 pKa = 6.06GEE555 pKa = 4.33PQPEE559 pKa = 3.95DD560 pKa = 3.57LARR563 pKa = 11.84QPSSLSSTVQGDD575 pKa = 3.44DD576 pKa = 3.38TFPVQEE582 pKa = 4.51IDD584 pKa = 4.07QEE586 pKa = 4.72TEE588 pKa = 3.71GLAVAEE594 pKa = 4.65HH595 pKa = 7.21DD596 pKa = 3.94PEE598 pKa = 4.18QSNDD602 pKa = 3.18YY603 pKa = 10.27HH604 pKa = 7.59VVVDD608 pKa = 4.06EE609 pKa = 4.47EE610 pKa = 5.94DD611 pKa = 3.5YY612 pKa = 11.46AQYY615 pKa = 11.7ADD617 pKa = 3.87ADD619 pKa = 3.93GGFDD623 pKa = 3.39AEE625 pKa = 5.45AYY627 pKa = 8.07EE628 pKa = 4.92QYY630 pKa = 10.99DD631 pKa = 3.46EE632 pKa = 5.99QYY634 pKa = 11.51DD635 pKa = 3.74EE636 pKa = 4.64LQAPEE641 pKa = 4.12HH642 pKa = 6.29TEE644 pKa = 3.76NDD646 pKa = 3.65DD647 pKa = 3.53AQEE650 pKa = 4.24AFQSQSYY657 pKa = 10.61EE658 pKa = 4.04DD659 pKa = 5.39LDD661 pKa = 4.11TNNYY665 pKa = 9.45NVGSEE670 pKa = 4.1EE671 pKa = 4.15QATGHH676 pKa = 6.29YY677 pKa = 9.68DD678 pKa = 3.22GKK680 pKa = 9.08TAGVDD685 pKa = 3.66GNYY688 pKa = 8.54EE689 pKa = 3.95AQVTVAGGGSIVGDD703 pKa = 3.72DD704 pKa = 3.55EE705 pKa = 6.21LEE707 pKa = 4.3NFDD710 pKa = 5.56LADD713 pKa = 3.43ATADD717 pKa = 3.51VTSYY721 pKa = 11.56DD722 pKa = 3.57AVGDD726 pKa = 3.67NAFNDD731 pKa = 4.02QYY733 pKa = 11.76SHH735 pKa = 6.94NDD737 pKa = 3.25EE738 pKa = 4.49NDD740 pKa = 3.2GDD742 pKa = 4.16GQAQVAAVSADD753 pKa = 3.72VEE755 pKa = 4.45PVAALSSEE763 pKa = 4.18SLNLSPQGQKK773 pKa = 8.78RR774 pKa = 11.84TIDD777 pKa = 3.43EE778 pKa = 4.17VGNDD782 pKa = 3.22VGEE785 pKa = 4.39ATDD788 pKa = 3.92PSDD791 pKa = 3.48AKK793 pKa = 10.52RR794 pKa = 11.84PRR796 pKa = 11.84VV797 pKa = 3.36

Molecular weight: 86.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V1DCG3|A0A2V1DCG3_9PLEO Uncharacterized protein OS=Periconia macrospinosa OX=97972 GN=DM02DRAFT_138206 PE=4 SV=1
MM1 pKa = 7.39ILSNRR6 pKa = 11.84RR7 pKa = 11.84PQPNQNPLHH16 pKa = 7.04RR17 pKa = 11.84IQLQNLPQNPLRR29 pKa = 11.84HH30 pKa = 5.04QLKK33 pKa = 8.92MLRR36 pKa = 11.84QIQPRR41 pKa = 11.84HH42 pKa = 5.54KK43 pKa = 10.24RR44 pKa = 11.84RR45 pKa = 11.84NLPSRR50 pKa = 11.84QRR52 pKa = 11.84RR53 pKa = 11.84SILPRR58 pKa = 11.84APEE61 pKa = 4.03TLPPNHH67 pKa = 6.91LLHH70 pKa = 6.34RR71 pKa = 11.84QNRR74 pKa = 11.84PPRR77 pKa = 11.84LGNHH81 pKa = 6.66PPRR84 pKa = 11.84QRR86 pKa = 11.84RR87 pKa = 11.84QRR89 pKa = 11.84QHH91 pKa = 6.94PLPLPNLLLRR101 pKa = 11.84RR102 pKa = 11.84HH103 pKa = 6.24HH104 pKa = 6.52PVPVPLPQRR113 pKa = 11.84LRR115 pKa = 11.84APGGPLHH122 pKa = 6.28GALRR126 pKa = 11.84RR127 pKa = 11.84LHH129 pKa = 6.2RR130 pKa = 11.84RR131 pKa = 11.84RR132 pKa = 11.84SRR134 pKa = 11.84RR135 pKa = 11.84LGAPFIHH142 pKa = 6.72PHH144 pKa = 5.71HH145 pKa = 6.96RR146 pKa = 11.84PHH148 pKa = 7.52LGPRR152 pKa = 11.84PQRR155 pKa = 11.84PNPNRR160 pKa = 11.84PLPHH164 pKa = 7.09PSKK167 pKa = 10.72SPPRR171 pKa = 11.84RR172 pKa = 11.84QQRR175 pKa = 11.84RR176 pKa = 11.84HH177 pKa = 5.91LPFAPFPRR185 pKa = 11.84HH186 pKa = 5.27LPPRR190 pKa = 11.84RR191 pKa = 11.84LQILRR196 pKa = 11.84RR197 pKa = 11.84IHH199 pKa = 5.94HH200 pKa = 6.64LVRR203 pKa = 11.84ARR205 pKa = 11.84RR206 pKa = 3.48

Molecular weight: 24.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18722

0

18722

7677607

49

8697

410.1

45.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.361 ± 0.016

1.364 ± 0.007

5.422 ± 0.014

6.025 ± 0.016

3.871 ± 0.01

6.687 ± 0.02

2.514 ± 0.008

5.098 ± 0.011

5.006 ± 0.014

8.936 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.176 ± 0.007

3.868 ± 0.011

5.992 ± 0.018

3.954 ± 0.015

5.958 ± 0.015

8.138 ± 0.018

6.116 ± 0.013

6.071 ± 0.014

1.57 ± 0.007

2.872 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski