Amino acid dipepetide frequency for Staphylococcus prophage phiPV83

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.003AlaAla: 2.003 ± 0.726
0.231AlaCys: 0.231 ± 0.134
2.312AlaAsp: 2.312 ± 0.387
3.005AlaGlu: 3.005 ± 0.498
2.08AlaPhe: 2.08 ± 0.606
3.313AlaGly: 3.313 ± 0.6
0.771AlaHis: 0.771 ± 0.271
4.623AlaIle: 4.623 ± 0.605
4.931AlaLys: 4.931 ± 0.666
4.469AlaLeu: 4.469 ± 0.688
1.541AlaMet: 1.541 ± 0.361
2.312AlaAsn: 2.312 ± 0.311
1.31AlaPro: 1.31 ± 0.283
2.466AlaGln: 2.466 ± 0.41
3.39AlaArg: 3.39 ± 0.54
3.236AlaSer: 3.236 ± 0.541
3.93AlaThr: 3.93 ± 0.591
3.082AlaVal: 3.082 ± 0.498
0.385AlaTrp: 0.385 ± 0.145
2.389AlaTyr: 2.389 ± 0.369
0.0AlaXaa: 0.0 ± 0.0
Cys
0.308CysAla: 0.308 ± 0.144
0.0CysCys: 0.0 ± 0.0
0.385CysAsp: 0.385 ± 0.184
0.308CysGlu: 0.308 ± 0.178
0.231CysPhe: 0.231 ± 0.143
0.462CysGly: 0.462 ± 0.224
0.231CysHis: 0.231 ± 0.122
0.539CysIle: 0.539 ± 0.2
0.462CysLys: 0.462 ± 0.165
0.771CysLeu: 0.771 ± 0.233
0.077CysMet: 0.077 ± 0.071
0.231CysAsn: 0.231 ± 0.128
0.154CysPro: 0.154 ± 0.102
0.0CysGln: 0.0 ± 0.0
0.231CysArg: 0.231 ± 0.132
0.462CysSer: 0.462 ± 0.208
0.308CysThr: 0.308 ± 0.146
0.231CysVal: 0.231 ± 0.131
0.0CysTrp: 0.0 ± 0.0
0.385CysTyr: 0.385 ± 0.155
0.0CysXaa: 0.0 ± 0.0
Asp
2.774AspAla: 2.774 ± 0.369
0.308AspCys: 0.308 ± 0.175
5.24AspAsp: 5.24 ± 0.666
5.008AspGlu: 5.008 ± 0.65
3.159AspPhe: 3.159 ± 0.47
3.39AspGly: 3.39 ± 0.613
0.848AspHis: 0.848 ± 0.247
4.7AspIle: 4.7 ± 0.595
5.856AspLys: 5.856 ± 0.956
5.471AspLeu: 5.471 ± 0.731
1.772AspMet: 1.772 ± 0.419
4.315AspAsn: 4.315 ± 0.541
1.695AspPro: 1.695 ± 0.373
0.925AspGln: 0.925 ± 0.292
2.928AspArg: 2.928 ± 0.504
4.238AspSer: 4.238 ± 0.584
3.313AspThr: 3.313 ± 0.564
3.93AspVal: 3.93 ± 0.506
0.539AspTrp: 0.539 ± 0.218
2.697AspTyr: 2.697 ± 0.355
0.0AspXaa: 0.0 ± 0.0
Glu
4.546GluAla: 4.546 ± 0.694
0.385GluCys: 0.385 ± 0.146
3.236GluAsp: 3.236 ± 0.467
5.548GluGlu: 5.548 ± 0.715
3.699GluPhe: 3.699 ± 0.507
2.003GluGly: 2.003 ± 0.322
0.848GluHis: 0.848 ± 0.217
6.164GluIle: 6.164 ± 0.822
6.164GluLys: 6.164 ± 0.803
7.243GluLeu: 7.243 ± 0.761
2.543GluMet: 2.543 ± 0.428
4.7GluAsn: 4.7 ± 0.637
1.849GluPro: 1.849 ± 0.332
3.544GluGln: 3.544 ± 0.559
4.238GluArg: 4.238 ± 0.544
4.007GluSer: 4.007 ± 0.536
3.776GluThr: 3.776 ± 0.536
4.546GluVal: 4.546 ± 0.667
0.771GluTrp: 0.771 ± 0.233
3.93GluTyr: 3.93 ± 0.742
0.0GluXaa: 0.0 ± 0.0
Phe
2.389PheAla: 2.389 ± 0.439
0.154PheCys: 0.154 ± 0.092
3.082PheAsp: 3.082 ± 0.454
2.389PheGlu: 2.389 ± 0.391
1.464PhePhe: 1.464 ± 0.46
2.774PheGly: 2.774 ± 0.515
0.539PheHis: 0.539 ± 0.21
3.622PheIle: 3.622 ± 0.641
3.776PheLys: 3.776 ± 0.511
2.697PheLeu: 2.697 ± 0.554
1.387PheMet: 1.387 ± 0.309
3.776PheAsn: 3.776 ± 0.481
1.002PhePro: 1.002 ± 0.327
1.387PheGln: 1.387 ± 0.391
1.772PheArg: 1.772 ± 0.299
2.389PheSer: 2.389 ± 0.369
2.62PheThr: 2.62 ± 0.487
2.697PheVal: 2.697 ± 0.548
0.231PheTrp: 0.231 ± 0.123
1.618PheTyr: 1.618 ± 0.458
0.0PheXaa: 0.0 ± 0.0
Gly
2.543GlyAla: 2.543 ± 0.891
0.462GlyCys: 0.462 ± 0.171
2.774GlyAsp: 2.774 ± 0.434
3.082GlyGlu: 3.082 ± 0.409
2.62GlyPhe: 2.62 ± 0.644
2.697GlyGly: 2.697 ± 0.575
1.233GlyHis: 1.233 ± 0.304
4.546GlyIle: 4.546 ± 0.831
5.471GlyLys: 5.471 ± 0.696
4.931GlyLeu: 4.931 ± 0.847
1.464GlyMet: 1.464 ± 0.394
2.774GlyAsn: 2.774 ± 0.549
0.771GlyPro: 0.771 ± 0.345
2.08GlyGln: 2.08 ± 0.485
2.543GlyArg: 2.543 ± 0.394
3.467GlySer: 3.467 ± 0.509
2.697GlyThr: 2.697 ± 0.444
3.467GlyVal: 3.467 ± 0.759
1.079GlyTrp: 1.079 ± 0.282
3.159GlyTyr: 3.159 ± 0.494
0.0GlyXaa: 0.0 ± 0.0
His
0.848HisAla: 0.848 ± 0.284
0.154HisCys: 0.154 ± 0.126
0.925HisAsp: 0.925 ± 0.282
1.233HisGlu: 1.233 ± 0.217
0.771HisPhe: 0.771 ± 0.221
0.616HisGly: 0.616 ± 0.265
0.154HisHis: 0.154 ± 0.102
1.618HisIle: 1.618 ± 0.371
1.002HisLys: 1.002 ± 0.352
1.31HisLeu: 1.31 ± 0.337
0.385HisMet: 0.385 ± 0.159
1.31HisAsn: 1.31 ± 0.306
0.385HisPro: 0.385 ± 0.155
0.925HisGln: 0.925 ± 0.242
0.616HisArg: 0.616 ± 0.175
1.079HisSer: 1.079 ± 0.272
1.387HisThr: 1.387 ± 0.369
0.616HisVal: 0.616 ± 0.215
0.308HisTrp: 0.308 ± 0.184
0.771HisTyr: 0.771 ± 0.285
0.0HisXaa: 0.0 ± 0.0
Ile
4.623IleAla: 4.623 ± 0.46
0.385IleCys: 0.385 ± 0.151
5.317IleAsp: 5.317 ± 0.706
6.781IleGlu: 6.781 ± 0.669
3.236IlePhe: 3.236 ± 0.696
4.315IleGly: 4.315 ± 0.573
1.387IleHis: 1.387 ± 0.404
5.394IleIle: 5.394 ± 0.694
7.012IleLys: 7.012 ± 0.742
4.623IleLeu: 4.623 ± 0.62
1.618IleMet: 1.618 ± 0.346
6.395IleAsn: 6.395 ± 0.889
1.926IlePro: 1.926 ± 0.429
3.313IleGln: 3.313 ± 0.487
3.467IleArg: 3.467 ± 0.489
4.084IleSer: 4.084 ± 0.566
5.086IleThr: 5.086 ± 0.558
5.008IleVal: 5.008 ± 0.477
0.925IleTrp: 0.925 ± 0.451
2.774IleTyr: 2.774 ± 0.493
0.0IleXaa: 0.0 ± 0.0
Lys
5.625LysAla: 5.625 ± 0.598
0.385LysCys: 0.385 ± 0.193
6.164LysAsp: 6.164 ± 0.602
7.782LysGlu: 7.782 ± 0.947
3.776LysPhe: 3.776 ± 0.471
5.317LysGly: 5.317 ± 0.696
1.772LysHis: 1.772 ± 0.351
5.702LysIle: 5.702 ± 0.743
7.782LysLys: 7.782 ± 0.938
8.707LysLeu: 8.707 ± 0.89
2.157LysMet: 2.157 ± 0.357
4.931LysAsn: 4.931 ± 0.533
2.389LysPro: 2.389 ± 0.465
4.315LysGln: 4.315 ± 0.614
4.7LysArg: 4.7 ± 0.539
5.317LysSer: 5.317 ± 0.536
5.394LysThr: 5.394 ± 0.72
5.163LysVal: 5.163 ± 0.695
1.233LysTrp: 1.233 ± 0.314
4.315LysTyr: 4.315 ± 0.533
0.0LysXaa: 0.0 ± 0.0
Leu
3.776LeuAla: 3.776 ± 0.54
0.616LeuCys: 0.616 ± 0.269
5.317LeuAsp: 5.317 ± 0.637
5.471LeuGlu: 5.471 ± 0.909
3.699LeuPhe: 3.699 ± 0.484
4.392LeuGly: 4.392 ± 0.78
1.079LeuHis: 1.079 ± 0.241
6.395LeuIle: 6.395 ± 0.679
8.399LeuLys: 8.399 ± 1.0
5.856LeuLeu: 5.856 ± 0.851
1.695LeuMet: 1.695 ± 0.323
6.627LeuAsn: 6.627 ± 0.644
3.082LeuPro: 3.082 ± 0.439
2.851LeuGln: 2.851 ± 0.387
4.161LeuArg: 4.161 ± 0.568
5.856LeuSer: 5.856 ± 0.686
4.7LeuThr: 4.7 ± 0.583
3.853LeuVal: 3.853 ± 0.39
0.693LeuTrp: 0.693 ± 0.213
3.005LeuTyr: 3.005 ± 0.439
0.0LeuXaa: 0.0 ± 0.0
Met
1.464MetAla: 1.464 ± 0.34
0.077MetCys: 0.077 ± 0.073
1.233MetAsp: 1.233 ± 0.262
1.772MetGlu: 1.772 ± 0.475
1.156MetPhe: 1.156 ± 0.276
1.079MetGly: 1.079 ± 0.416
0.539MetHis: 0.539 ± 0.248
2.235MetIle: 2.235 ± 0.262
2.389MetLys: 2.389 ± 0.436
2.235MetLeu: 2.235 ± 0.387
0.616MetMet: 0.616 ± 0.254
2.08MetAsn: 2.08 ± 0.469
1.31MetPro: 1.31 ± 0.383
0.848MetGln: 0.848 ± 0.27
1.233MetArg: 1.233 ± 0.335
1.849MetSer: 1.849 ± 0.327
1.31MetThr: 1.31 ± 0.276
1.541MetVal: 1.541 ± 0.379
0.462MetTrp: 0.462 ± 0.153
1.079MetTyr: 1.079 ± 0.3
0.0MetXaa: 0.0 ± 0.0
Asn
3.159AsnAla: 3.159 ± 0.45
0.308AsnCys: 0.308 ± 0.17
4.392AsnAsp: 4.392 ± 0.747
4.931AsnGlu: 4.931 ± 0.55
1.849AsnPhe: 1.849 ± 0.436
5.086AsnGly: 5.086 ± 0.806
1.233AsnHis: 1.233 ± 0.296
4.469AsnIle: 4.469 ± 0.548
6.164AsnLys: 6.164 ± 0.701
5.548AsnLeu: 5.548 ± 0.719
1.926AsnMet: 1.926 ± 0.337
5.548AsnAsn: 5.548 ± 0.848
2.389AsnPro: 2.389 ± 0.373
3.467AsnGln: 3.467 ± 0.499
2.928AsnArg: 2.928 ± 0.482
3.622AsnSer: 3.622 ± 0.479
4.161AsnThr: 4.161 ± 0.507
4.084AsnVal: 4.084 ± 0.628
1.464AsnTrp: 1.464 ± 0.367
3.159AsnTyr: 3.159 ± 0.471
0.0AsnXaa: 0.0 ± 0.0
Pro
0.925ProAla: 0.925 ± 0.27
0.0ProCys: 0.0 ± 0.0
1.156ProAsp: 1.156 ± 0.32
2.389ProGlu: 2.389 ± 0.456
1.31ProPhe: 1.31 ± 0.251
0.848ProGly: 0.848 ± 0.213
0.616ProHis: 0.616 ± 0.222
2.389ProIle: 2.389 ± 0.325
3.467ProLys: 3.467 ± 0.55
2.312ProLeu: 2.312 ± 0.47
0.693ProMet: 0.693 ± 0.278
1.926ProAsn: 1.926 ± 0.363
0.848ProPro: 0.848 ± 0.193
0.925ProGln: 0.925 ± 0.271
0.616ProArg: 0.616 ± 0.198
1.926ProSer: 1.926 ± 0.475
1.541ProThr: 1.541 ± 0.351
1.618ProVal: 1.618 ± 0.339
0.154ProTrp: 0.154 ± 0.1
1.002ProTyr: 1.002 ± 0.259
0.0ProXaa: 0.0 ± 0.0
Gln
3.313GlnAla: 3.313 ± 0.483
0.616GlnCys: 0.616 ± 0.234
2.466GlnAsp: 2.466 ± 0.466
2.851GlnGlu: 2.851 ± 0.653
1.387GlnPhe: 1.387 ± 0.274
1.772GlnGly: 1.772 ± 0.434
0.848GlnHis: 0.848 ± 0.251
3.236GlnIle: 3.236 ± 0.437
3.39GlnLys: 3.39 ± 0.499
3.159GlnLeu: 3.159 ± 0.56
1.002GlnMet: 1.002 ± 0.318
3.853GlnAsn: 3.853 ± 0.705
1.002GlnPro: 1.002 ± 0.257
2.08GlnGln: 2.08 ± 0.479
2.003GlnArg: 2.003 ± 0.334
1.926GlnSer: 1.926 ± 0.461
1.695GlnThr: 1.695 ± 0.313
1.618GlnVal: 1.618 ± 0.412
0.385GlnTrp: 0.385 ± 0.204
1.849GlnTyr: 1.849 ± 0.343
0.0GlnXaa: 0.0 ± 0.0
Arg
2.312ArgAla: 2.312 ± 0.428
0.308ArgCys: 0.308 ± 0.17
3.159ArgAsp: 3.159 ± 0.437
4.007ArgGlu: 4.007 ± 0.533
1.541ArgPhe: 1.541 ± 0.353
2.312ArgGly: 2.312 ± 0.431
0.848ArgHis: 0.848 ± 0.191
3.39ArgIle: 3.39 ± 0.517
4.854ArgLys: 4.854 ± 0.545
4.161ArgLeu: 4.161 ± 0.66
1.31ArgMet: 1.31 ± 0.36
3.236ArgAsn: 3.236 ± 0.394
0.925ArgPro: 0.925 ± 0.317
2.003ArgGln: 2.003 ± 0.436
1.772ArgArg: 1.772 ± 0.479
2.003ArgSer: 2.003 ± 0.404
3.236ArgThr: 3.236 ± 0.455
2.543ArgVal: 2.543 ± 0.436
0.539ArgTrp: 0.539 ± 0.21
2.466ArgTyr: 2.466 ± 0.648
0.0ArgXaa: 0.0 ± 0.0
Ser
3.005SerAla: 3.005 ± 0.521
0.308SerCys: 0.308 ± 0.168
4.623SerAsp: 4.623 ± 0.689
5.24SerGlu: 5.24 ± 0.904
2.235SerPhe: 2.235 ± 0.462
3.313SerGly: 3.313 ± 0.703
1.079SerHis: 1.079 ± 0.257
5.317SerIle: 5.317 ± 0.515
5.702SerLys: 5.702 ± 0.883
4.469SerLeu: 4.469 ± 0.571
1.464SerMet: 1.464 ± 0.377
4.623SerAsn: 4.623 ± 0.656
0.616SerPro: 0.616 ± 0.185
2.389SerGln: 2.389 ± 0.432
2.774SerArg: 2.774 ± 0.455
3.467SerSer: 3.467 ± 0.656
2.08SerThr: 2.08 ± 0.542
3.313SerVal: 3.313 ± 0.564
0.462SerTrp: 0.462 ± 0.165
3.39SerTyr: 3.39 ± 0.512
0.0SerXaa: 0.0 ± 0.0
Thr
2.774ThrAla: 2.774 ± 0.403
0.231ThrCys: 0.231 ± 0.127
4.392ThrAsp: 4.392 ± 0.836
3.467ThrGlu: 3.467 ± 0.537
2.235ThrPhe: 2.235 ± 0.462
3.622ThrGly: 3.622 ± 0.703
1.156ThrHis: 1.156 ± 0.258
5.008ThrIle: 5.008 ± 0.534
4.161ThrLys: 4.161 ± 0.754
4.623ThrLeu: 4.623 ± 0.595
1.464ThrMet: 1.464 ± 0.36
2.543ThrAsn: 2.543 ± 0.36
2.08ThrPro: 2.08 ± 0.319
2.157ThrGln: 2.157 ± 0.362
2.928ThrArg: 2.928 ± 0.46
3.93ThrSer: 3.93 ± 0.627
3.699ThrThr: 3.699 ± 0.639
3.699ThrVal: 3.699 ± 0.452
0.848ThrTrp: 0.848 ± 0.238
2.312ThrTyr: 2.312 ± 0.552
0.0ThrXaa: 0.0 ± 0.0
Val
2.851ValAla: 2.851 ± 0.418
0.308ValCys: 0.308 ± 0.192
3.853ValAsp: 3.853 ± 0.553
3.93ValGlu: 3.93 ± 0.579
2.543ValPhe: 2.543 ± 0.456
3.776ValGly: 3.776 ± 0.736
0.385ValHis: 0.385 ± 0.16
3.853ValIle: 3.853 ± 0.474
6.472ValLys: 6.472 ± 0.669
4.854ValLeu: 4.854 ± 0.551
1.387ValMet: 1.387 ± 0.567
4.315ValAsn: 4.315 ± 0.404
1.541ValPro: 1.541 ± 0.281
1.926ValGln: 1.926 ± 0.348
2.08ValArg: 2.08 ± 0.415
3.699ValSer: 3.699 ± 0.53
4.007ValThr: 4.007 ± 0.544
3.467ValVal: 3.467 ± 0.566
0.462ValTrp: 0.462 ± 0.169
2.08ValTyr: 2.08 ± 0.364
0.0ValXaa: 0.0 ± 0.0
Trp
0.462TrpAla: 0.462 ± 0.237
0.077TrpCys: 0.077 ± 0.068
0.616TrpAsp: 0.616 ± 0.208
0.771TrpGlu: 0.771 ± 0.18
0.771TrpPhe: 0.771 ± 0.211
0.539TrpGly: 0.539 ± 0.282
0.154TrpHis: 0.154 ± 0.089
1.079TrpIle: 1.079 ± 0.315
0.925TrpLys: 0.925 ± 0.262
0.771TrpLeu: 0.771 ± 0.31
0.616TrpMet: 0.616 ± 0.205
0.925TrpAsn: 0.925 ± 0.482
0.231TrpPro: 0.231 ± 0.129
0.616TrpGln: 0.616 ± 0.175
0.771TrpArg: 0.771 ± 0.202
0.616TrpSer: 0.616 ± 0.198
0.385TrpThr: 0.385 ± 0.117
1.002TrpVal: 1.002 ± 0.223
0.231TrpTrp: 0.231 ± 0.125
0.385TrpTyr: 0.385 ± 0.193
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.08TyrAla: 2.08 ± 0.35
0.462TyrCys: 0.462 ± 0.162
2.466TyrAsp: 2.466 ± 0.35
3.776TyrGlu: 3.776 ± 0.51
2.003TyrPhe: 2.003 ± 0.418
2.312TyrGly: 2.312 ± 0.504
0.693TyrHis: 0.693 ± 0.214
3.39TyrIle: 3.39 ± 0.528
4.546TyrLys: 4.546 ± 0.647
3.313TyrLeu: 3.313 ± 0.472
1.31TyrMet: 1.31 ± 0.285
3.467TyrAsn: 3.467 ± 0.498
1.156TyrPro: 1.156 ± 0.233
2.235TyrGln: 2.235 ± 0.335
1.772TyrArg: 1.772 ± 0.366
2.697TyrSer: 2.697 ± 0.498
2.003TyrThr: 2.003 ± 0.408
2.312TyrVal: 2.312 ± 0.399
0.771TyrTrp: 0.771 ± 0.218
1.31TyrTyr: 1.31 ± 0.351
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (12979 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski