Amino acid dipepetide frequency for Streptococcus phage phi20c

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.528AlaAla: 3.528 ± 0.662
0.572AlaCys: 0.572 ± 0.248
4.767AlaAsp: 4.767 ± 0.618
7.628AlaGlu: 7.628 ± 1.017
2.288AlaPhe: 2.288 ± 0.391
5.912AlaGly: 5.912 ± 0.95
1.049AlaHis: 1.049 ± 0.356
6.579AlaIle: 6.579 ± 0.948
7.532AlaLys: 7.532 ± 0.863
6.102AlaLeu: 6.102 ± 0.706
1.907AlaMet: 1.907 ± 0.425
5.339AlaAsn: 5.339 ± 0.721
0.858AlaPro: 0.858 ± 0.271
3.623AlaGln: 3.623 ± 0.716
2.67AlaArg: 2.67 ± 0.534
4.672AlaSer: 4.672 ± 0.813
4.672AlaThr: 4.672 ± 0.775
4.672AlaVal: 4.672 ± 0.64
0.858AlaTrp: 0.858 ± 0.324
1.812AlaTyr: 1.812 ± 0.379
0.0AlaXaa: 0.0 ± 0.0
Cys
0.477CysAla: 0.477 ± 0.259
0.191CysCys: 0.191 ± 0.128
0.477CysAsp: 0.477 ± 0.201
0.381CysGlu: 0.381 ± 0.198
0.191CysPhe: 0.191 ± 0.132
0.477CysGly: 0.477 ± 0.259
0.286CysHis: 0.286 ± 0.157
0.286CysIle: 0.286 ± 0.166
0.286CysLys: 0.286 ± 0.196
0.572CysLeu: 0.572 ± 0.227
0.0CysMet: 0.0 ± 0.0
0.095CysAsn: 0.095 ± 0.104
0.191CysPro: 0.191 ± 0.128
0.191CysGln: 0.191 ± 0.137
0.667CysArg: 0.667 ± 0.291
0.572CysSer: 0.572 ± 0.252
0.286CysThr: 0.286 ± 0.165
0.477CysVal: 0.477 ± 0.193
0.095CysTrp: 0.095 ± 0.1
0.477CysTyr: 0.477 ± 0.24
0.0CysXaa: 0.0 ± 0.0
Asp
3.337AspAla: 3.337 ± 0.608
0.572AspCys: 0.572 ± 0.268
3.242AspAsp: 3.242 ± 0.572
4.195AspGlu: 4.195 ± 0.759
3.432AspPhe: 3.432 ± 0.687
4.672AspGly: 4.672 ± 0.634
0.858AspHis: 0.858 ± 0.283
4.863AspIle: 4.863 ± 0.652
4.481AspLys: 4.481 ± 0.628
5.816AspLeu: 5.816 ± 0.663
1.812AspMet: 1.812 ± 0.414
3.242AspAsn: 3.242 ± 0.555
1.526AspPro: 1.526 ± 0.353
1.335AspGln: 1.335 ± 0.35
3.337AspArg: 3.337 ± 0.625
3.623AspSer: 3.623 ± 0.562
2.86AspThr: 2.86 ± 0.447
4.386AspVal: 4.386 ± 0.67
1.049AspTrp: 1.049 ± 0.312
2.193AspTyr: 2.193 ± 0.608
0.0AspXaa: 0.0 ± 0.0
Glu
6.293GluAla: 6.293 ± 0.911
0.286GluCys: 0.286 ± 0.176
3.242GluAsp: 3.242 ± 0.651
4.958GluGlu: 4.958 ± 0.92
2.479GluPhe: 2.479 ± 0.453
3.909GluGly: 3.909 ± 0.595
1.335GluHis: 1.335 ± 0.431
5.625GluIle: 5.625 ± 0.97
5.053GluLys: 5.053 ± 0.856
7.151GluLeu: 7.151 ± 1.027
3.146GluMet: 3.146 ± 0.555
4.863GluAsn: 4.863 ± 0.82
1.526GluPro: 1.526 ± 0.477
2.956GluGln: 2.956 ± 0.492
4.577GluArg: 4.577 ± 0.711
4.195GluSer: 4.195 ± 0.698
4.1GluThr: 4.1 ± 0.592
4.767GluVal: 4.767 ± 0.639
0.667GluTrp: 0.667 ± 0.216
2.384GluTyr: 2.384 ± 0.518
0.0GluXaa: 0.0 ± 0.0
Phe
3.337PheAla: 3.337 ± 0.598
0.095PheCys: 0.095 ± 0.092
3.909PheAsp: 3.909 ± 0.51
5.244PheGlu: 5.244 ± 0.816
1.716PhePhe: 1.716 ± 0.367
3.623PheGly: 3.623 ± 0.611
0.381PheHis: 0.381 ± 0.167
2.479PheIle: 2.479 ± 0.527
2.67PheLys: 2.67 ± 0.379
2.288PheLeu: 2.288 ± 0.5
1.335PheMet: 1.335 ± 0.367
2.193PheAsn: 2.193 ± 0.405
1.335PhePro: 1.335 ± 0.309
0.572PheGln: 0.572 ± 0.284
1.335PheArg: 1.335 ± 0.49
1.812PheSer: 1.812 ± 0.452
2.384PheThr: 2.384 ± 0.51
2.384PheVal: 2.384 ± 0.457
0.381PheTrp: 0.381 ± 0.167
1.621PheTyr: 1.621 ± 0.338
0.0PheXaa: 0.0 ± 0.0
Gly
5.244GlyAla: 5.244 ± 1.04
0.095GlyCys: 0.095 ± 0.086
3.623GlyAsp: 3.623 ± 0.576
3.909GlyGlu: 3.909 ± 0.688
2.86GlyPhe: 2.86 ± 0.612
3.909GlyGly: 3.909 ± 0.761
0.953GlyHis: 0.953 ± 0.277
4.291GlyIle: 4.291 ± 0.689
5.53GlyLys: 5.53 ± 0.778
5.435GlyLeu: 5.435 ± 0.901
2.098GlyMet: 2.098 ± 0.392
3.623GlyAsn: 3.623 ± 0.856
0.858GlyPro: 0.858 ± 0.27
3.909GlyGln: 3.909 ± 0.579
3.051GlyArg: 3.051 ± 0.449
3.814GlySer: 3.814 ± 0.804
3.432GlyThr: 3.432 ± 0.625
4.1GlyVal: 4.1 ± 0.76
0.763GlyTrp: 0.763 ± 0.27
2.765GlyTyr: 2.765 ± 0.594
0.0GlyXaa: 0.0 ± 0.0
His
0.858HisAla: 0.858 ± 0.249
0.095HisCys: 0.095 ± 0.104
0.858HisAsp: 0.858 ± 0.334
0.953HisGlu: 0.953 ± 0.28
0.763HisPhe: 0.763 ± 0.3
1.144HisGly: 1.144 ± 0.279
0.381HisHis: 0.381 ± 0.178
0.858HisIle: 0.858 ± 0.277
0.763HisLys: 0.763 ± 0.275
1.24HisLeu: 1.24 ± 0.315
0.477HisMet: 0.477 ± 0.189
0.763HisAsn: 0.763 ± 0.249
1.144HisPro: 1.144 ± 0.308
0.572HisGln: 0.572 ± 0.205
0.667HisArg: 0.667 ± 0.253
0.667HisSer: 0.667 ± 0.234
0.477HisThr: 0.477 ± 0.22
0.953HisVal: 0.953 ± 0.337
0.095HisTrp: 0.095 ± 0.094
0.477HisTyr: 0.477 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
5.435IleAla: 5.435 ± 0.533
0.572IleCys: 0.572 ± 0.272
5.912IleAsp: 5.912 ± 0.845
5.53IleGlu: 5.53 ± 0.769
2.765IlePhe: 2.765 ± 0.456
4.481IleGly: 4.481 ± 0.538
0.572IleHis: 0.572 ± 0.211
4.291IleIle: 4.291 ± 0.809
6.293IleLys: 6.293 ± 0.66
3.814IleLeu: 3.814 ± 0.516
1.812IleMet: 1.812 ± 0.533
3.337IleAsn: 3.337 ± 0.978
2.098IlePro: 2.098 ± 0.545
3.242IleGln: 3.242 ± 0.679
2.479IleArg: 2.479 ± 0.557
4.005IleSer: 4.005 ± 0.842
4.1IleThr: 4.1 ± 0.645
4.005IleVal: 4.005 ± 0.657
0.763IleTrp: 0.763 ± 0.28
2.193IleTyr: 2.193 ± 0.5
0.0IleXaa: 0.0 ± 0.0
Lys
5.625LysAla: 5.625 ± 0.668
0.286LysCys: 0.286 ± 0.19
3.814LysAsp: 3.814 ± 0.609
5.053LysGlu: 5.053 ± 0.84
2.67LysPhe: 2.67 ± 0.576
4.672LysGly: 4.672 ± 0.638
1.43LysHis: 1.43 ± 0.455
5.53LysIle: 5.53 ± 0.684
7.342LysLys: 7.342 ± 1.319
5.053LysLeu: 5.053 ± 0.581
2.193LysMet: 2.193 ± 0.372
4.672LysAsn: 4.672 ± 0.718
2.86LysPro: 2.86 ± 0.506
4.863LysGln: 4.863 ± 0.887
4.767LysArg: 4.767 ± 0.681
4.863LysSer: 4.863 ± 0.659
5.149LysThr: 5.149 ± 0.863
5.053LysVal: 5.053 ± 1.101
1.144LysTrp: 1.144 ± 0.359
2.67LysTyr: 2.67 ± 0.572
0.0LysXaa: 0.0 ± 0.0
Leu
8.486LeuAla: 8.486 ± 0.796
0.667LeuCys: 0.667 ± 0.244
4.195LeuAsp: 4.195 ± 0.588
6.674LeuGlu: 6.674 ± 0.982
3.719LeuPhe: 3.719 ± 0.546
4.767LeuGly: 4.767 ± 0.858
1.049LeuHis: 1.049 ± 0.347
3.528LeuIle: 3.528 ± 0.447
7.342LeuLys: 7.342 ± 0.877
7.437LeuLeu: 7.437 ± 0.853
1.621LeuMet: 1.621 ± 0.398
4.577LeuAsn: 4.577 ± 0.595
2.956LeuPro: 2.956 ± 0.569
2.574LeuGln: 2.574 ± 0.505
3.909LeuArg: 3.909 ± 0.636
4.672LeuSer: 4.672 ± 0.897
5.339LeuThr: 5.339 ± 0.604
4.863LeuVal: 4.863 ± 0.69
0.763LeuTrp: 0.763 ± 0.26
1.24LeuTyr: 1.24 ± 0.4
0.0LeuXaa: 0.0 ± 0.0
Met
2.479MetAla: 2.479 ± 0.478
0.381MetCys: 0.381 ± 0.193
1.526MetAsp: 1.526 ± 0.374
1.526MetGlu: 1.526 ± 0.307
0.572MetPhe: 0.572 ± 0.166
1.526MetGly: 1.526 ± 0.596
0.191MetHis: 0.191 ± 0.108
2.098MetIle: 2.098 ± 0.579
2.288MetLys: 2.288 ± 0.52
1.812MetLeu: 1.812 ± 0.366
0.095MetMet: 0.095 ± 0.118
1.335MetAsn: 1.335 ± 0.391
1.049MetPro: 1.049 ± 0.271
0.667MetGln: 0.667 ± 0.211
0.858MetArg: 0.858 ± 0.254
2.002MetSer: 2.002 ± 0.416
3.051MetThr: 3.051 ± 0.437
1.716MetVal: 1.716 ± 0.402
0.381MetTrp: 0.381 ± 0.169
0.858MetTyr: 0.858 ± 0.331
0.0MetXaa: 0.0 ± 0.0
Asn
3.623AsnAla: 3.623 ± 0.67
0.572AsnCys: 0.572 ± 0.244
2.384AsnAsp: 2.384 ± 0.573
2.288AsnGlu: 2.288 ± 0.385
2.288AsnPhe: 2.288 ± 0.435
4.005AsnGly: 4.005 ± 0.722
0.572AsnHis: 0.572 ± 0.211
4.291AsnIle: 4.291 ± 0.719
3.719AsnLys: 3.719 ± 0.601
5.339AsnLeu: 5.339 ± 0.887
0.763AsnMet: 0.763 ± 0.278
2.67AsnAsn: 2.67 ± 0.602
2.67AsnPro: 2.67 ± 0.566
3.623AsnGln: 3.623 ± 0.652
3.051AsnArg: 3.051 ± 0.545
3.528AsnSer: 3.528 ± 0.643
2.86AsnThr: 2.86 ± 0.601
2.86AsnVal: 2.86 ± 0.434
0.763AsnTrp: 0.763 ± 0.278
2.574AsnTyr: 2.574 ± 0.47
0.0AsnXaa: 0.0 ± 0.0
Pro
1.716ProAla: 1.716 ± 0.34
0.191ProCys: 0.191 ± 0.136
2.193ProAsp: 2.193 ± 0.525
2.193ProGlu: 2.193 ± 0.599
1.144ProPhe: 1.144 ± 0.305
1.144ProGly: 1.144 ± 0.276
0.572ProHis: 0.572 ± 0.22
2.193ProIle: 2.193 ± 0.397
2.574ProLys: 2.574 ± 0.638
2.288ProLeu: 2.288 ± 0.428
1.24ProMet: 1.24 ± 0.262
1.812ProAsn: 1.812 ± 0.44
0.381ProPro: 0.381 ± 0.183
0.858ProGln: 0.858 ± 0.28
0.858ProArg: 0.858 ± 0.295
2.384ProSer: 2.384 ± 0.522
2.193ProThr: 2.193 ± 0.526
1.812ProVal: 1.812 ± 0.405
0.286ProTrp: 0.286 ± 0.168
0.763ProTyr: 0.763 ± 0.324
0.0ProXaa: 0.0 ± 0.0
Gln
4.005GlnAla: 4.005 ± 0.527
0.191GlnCys: 0.191 ± 0.151
2.479GlnAsp: 2.479 ± 0.467
2.479GlnGlu: 2.479 ± 0.569
1.335GlnPhe: 1.335 ± 0.348
1.621GlnGly: 1.621 ± 0.393
0.477GlnHis: 0.477 ± 0.332
3.146GlnIle: 3.146 ± 0.551
2.956GlnLys: 2.956 ± 0.563
4.1GlnLeu: 4.1 ± 0.653
0.858GlnMet: 0.858 ± 0.244
1.526GlnAsn: 1.526 ± 0.438
1.335GlnPro: 1.335 ± 0.361
1.907GlnGln: 1.907 ± 0.521
2.098GlnArg: 2.098 ± 0.418
3.814GlnSer: 3.814 ± 0.63
3.051GlnThr: 3.051 ± 0.667
2.67GlnVal: 2.67 ± 0.41
0.477GlnTrp: 0.477 ± 0.203
1.24GlnTyr: 1.24 ± 0.308
0.0GlnXaa: 0.0 ± 0.0
Arg
2.574ArgAla: 2.574 ± 0.586
0.381ArgCys: 0.381 ± 0.237
2.384ArgAsp: 2.384 ± 0.482
4.1ArgGlu: 4.1 ± 0.739
2.288ArgPhe: 2.288 ± 0.367
1.907ArgGly: 1.907 ± 0.409
1.43ArgHis: 1.43 ± 0.413
4.1ArgIle: 4.1 ± 0.638
3.719ArgLys: 3.719 ± 0.767
4.577ArgLeu: 4.577 ± 0.676
1.144ArgMet: 1.144 ± 0.312
2.765ArgAsn: 2.765 ± 0.567
1.43ArgPro: 1.43 ± 0.45
1.335ArgGln: 1.335 ± 0.416
2.288ArgArg: 2.288 ± 0.522
2.288ArgSer: 2.288 ± 0.349
3.051ArgThr: 3.051 ± 0.58
3.242ArgVal: 3.242 ± 0.456
0.381ArgTrp: 0.381 ± 0.198
2.384ArgTyr: 2.384 ± 0.488
0.0ArgXaa: 0.0 ± 0.0
Ser
5.53SerAla: 5.53 ± 1.182
0.477SerCys: 0.477 ± 0.172
3.719SerAsp: 3.719 ± 0.531
4.386SerGlu: 4.386 ± 0.672
3.432SerPhe: 3.432 ± 0.712
4.863SerGly: 4.863 ± 0.819
0.477SerHis: 0.477 ± 0.192
3.909SerIle: 3.909 ± 0.677
4.1SerLys: 4.1 ± 0.683
4.767SerLeu: 4.767 ± 0.875
2.098SerMet: 2.098 ± 0.499
3.242SerAsn: 3.242 ± 0.426
1.43SerPro: 1.43 ± 0.354
2.574SerGln: 2.574 ± 0.606
2.765SerArg: 2.765 ± 0.511
4.195SerSer: 4.195 ± 0.798
3.242SerThr: 3.242 ± 0.58
4.863SerVal: 4.863 ± 1.024
1.049SerTrp: 1.049 ± 0.295
2.765SerTyr: 2.765 ± 0.44
0.0SerXaa: 0.0 ± 0.0
Thr
5.625ThrAla: 5.625 ± 1.037
0.095ThrCys: 0.095 ± 0.078
3.528ThrAsp: 3.528 ± 0.596
3.814ThrGlu: 3.814 ± 0.766
2.384ThrPhe: 2.384 ± 0.502
4.958ThrGly: 4.958 ± 0.652
0.953ThrHis: 0.953 ± 0.351
3.528ThrIle: 3.528 ± 0.633
4.291ThrLys: 4.291 ± 0.612
4.767ThrLeu: 4.767 ± 0.761
0.858ThrMet: 0.858 ± 0.282
2.956ThrAsn: 2.956 ± 0.513
1.335ThrPro: 1.335 ± 0.306
2.193ThrGln: 2.193 ± 0.569
2.574ThrArg: 2.574 ± 0.529
3.909ThrSer: 3.909 ± 0.522
2.67ThrThr: 2.67 ± 0.51
6.388ThrVal: 6.388 ± 0.981
0.381ThrTrp: 0.381 ± 0.224
2.384ThrTyr: 2.384 ± 0.406
0.0ThrXaa: 0.0 ± 0.0
Val
5.435ValAla: 5.435 ± 0.784
0.477ValCys: 0.477 ± 0.223
5.339ValAsp: 5.339 ± 0.717
5.053ValGlu: 5.053 ± 0.776
2.384ValPhe: 2.384 ± 0.487
3.719ValGly: 3.719 ± 0.591
0.667ValHis: 0.667 ± 0.241
3.719ValIle: 3.719 ± 0.52
4.958ValLys: 4.958 ± 0.743
4.577ValLeu: 4.577 ± 0.691
1.335ValMet: 1.335 ± 0.403
3.432ValAsn: 3.432 ± 0.518
2.288ValPro: 2.288 ± 0.42
2.86ValGln: 2.86 ± 0.569
2.765ValArg: 2.765 ± 0.53
5.816ValSer: 5.816 ± 0.767
3.623ValThr: 3.623 ± 0.845
3.909ValVal: 3.909 ± 0.635
0.858ValTrp: 0.858 ± 0.246
2.384ValTyr: 2.384 ± 0.526
0.0ValXaa: 0.0 ± 0.0
Trp
1.049TrpAla: 1.049 ± 0.315
0.191TrpCys: 0.191 ± 0.14
0.572TrpAsp: 0.572 ± 0.185
1.144TrpGlu: 1.144 ± 0.322
0.572TrpPhe: 0.572 ± 0.247
1.24TrpGly: 1.24 ± 0.328
0.0TrpHis: 0.0 ± 0.0
0.763TrpIle: 0.763 ± 0.292
1.144TrpLys: 1.144 ± 0.334
0.572TrpLeu: 0.572 ± 0.209
0.191TrpMet: 0.191 ± 0.119
0.667TrpAsn: 0.667 ± 0.234
0.095TrpPro: 0.095 ± 0.094
0.477TrpGln: 0.477 ± 0.306
0.667TrpArg: 0.667 ± 0.213
0.763TrpSer: 0.763 ± 0.242
0.763TrpThr: 0.763 ± 0.229
0.572TrpVal: 0.572 ± 0.221
0.095TrpTrp: 0.095 ± 0.086
0.381TrpTyr: 0.381 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.384TyrAla: 2.384 ± 0.483
0.286TyrCys: 0.286 ± 0.176
2.67TyrAsp: 2.67 ± 0.478
2.002TyrGlu: 2.002 ± 0.524
1.812TyrPhe: 1.812 ± 0.45
1.716TyrGly: 1.716 ± 0.484
0.572TyrHis: 0.572 ± 0.208
1.621TyrIle: 1.621 ± 0.431
2.67TyrLys: 2.67 ± 0.578
2.67TyrLeu: 2.67 ± 0.464
1.335TyrMet: 1.335 ± 0.413
1.335TyrAsn: 1.335 ± 0.345
1.43TyrPro: 1.43 ± 0.292
1.43TyrGln: 1.43 ± 0.33
2.384TyrArg: 2.384 ± 0.523
2.384TyrSer: 2.384 ± 0.569
2.288TyrThr: 2.288 ± 0.477
1.907TyrVal: 1.907 ± 0.271
0.667TyrTrp: 0.667 ± 0.248
0.858TyrTyr: 0.858 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (10489 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski