Amino acid dipepetide frequency for Arthrobacter phage Shepard

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.556AlaAla: 8.556 ± 1.416
0.329AlaCys: 0.329 ± 0.16
4.388AlaAsp: 4.388 ± 0.483
5.759AlaGlu: 5.759 ± 0.573
3.017AlaPhe: 3.017 ± 0.396
7.185AlaGly: 7.185 ± 1.263
1.481AlaHis: 1.481 ± 0.254
4.607AlaIle: 4.607 ± 0.464
6.308AlaLys: 6.308 ± 0.781
7.295AlaLeu: 7.295 ± 0.938
2.962AlaMet: 2.962 ± 0.61
3.949AlaAsn: 3.949 ± 0.491
2.742AlaPro: 2.742 ± 0.338
3.017AlaGln: 3.017 ± 0.426
5.211AlaArg: 5.211 ± 0.533
5.211AlaSer: 5.211 ± 0.726
6.033AlaThr: 6.033 ± 0.456
6.088AlaVal: 6.088 ± 0.849
1.645AlaTrp: 1.645 ± 0.333
2.358AlaTyr: 2.358 ± 0.315
0.0AlaXaa: 0.0 ± 0.0
Cys
0.274CysAla: 0.274 ± 0.138
0.165CysCys: 0.165 ± 0.107
0.658CysAsp: 0.658 ± 0.183
0.274CysGlu: 0.274 ± 0.115
0.384CysPhe: 0.384 ± 0.152
0.603CysGly: 0.603 ± 0.201
0.274CysHis: 0.274 ± 0.125
0.384CysIle: 0.384 ± 0.186
0.165CysLys: 0.165 ± 0.136
0.548CysLeu: 0.548 ± 0.2
0.165CysMet: 0.165 ± 0.08
0.11CysAsn: 0.11 ± 0.063
0.165CysPro: 0.165 ± 0.093
0.274CysGln: 0.274 ± 0.132
0.329CysArg: 0.329 ± 0.124
0.329CysSer: 0.329 ± 0.148
0.274CysThr: 0.274 ± 0.122
0.384CysVal: 0.384 ± 0.156
0.055CysTrp: 0.055 ± 0.054
0.11CysTyr: 0.11 ± 0.079
0.0CysXaa: 0.0 ± 0.0
Asp
5.649AspAla: 5.649 ± 0.579
0.329AspCys: 0.329 ± 0.13
3.62AspAsp: 3.62 ± 0.429
5.211AspGlu: 5.211 ± 0.626
2.304AspPhe: 2.304 ± 0.381
4.004AspGly: 4.004 ± 0.534
0.878AspHis: 0.878 ± 0.235
4.827AspIle: 4.827 ± 0.53
2.797AspLys: 2.797 ± 0.342
4.991AspLeu: 4.991 ± 0.571
1.7AspMet: 1.7 ± 0.31
3.291AspAsn: 3.291 ± 0.495
2.194AspPro: 2.194 ± 0.283
2.962AspGln: 2.962 ± 0.414
2.578AspArg: 2.578 ± 0.499
3.181AspSer: 3.181 ± 0.361
3.236AspThr: 3.236 ± 0.475
3.62AspVal: 3.62 ± 0.505
1.097AspTrp: 1.097 ± 0.228
2.029AspTyr: 2.029 ± 0.314
0.0AspXaa: 0.0 ± 0.0
Glu
5.54GluAla: 5.54 ± 0.628
0.768GluCys: 0.768 ± 0.25
3.73GluAsp: 3.73 ± 0.424
5.101GluGlu: 5.101 ± 0.69
2.797GluPhe: 2.797 ± 0.475
4.223GluGly: 4.223 ± 0.43
1.7GluHis: 1.7 ± 0.343
3.62GluIle: 3.62 ± 0.459
3.565GluLys: 3.565 ± 0.569
5.814GluLeu: 5.814 ± 0.546
2.194GluMet: 2.194 ± 0.32
2.578GluAsn: 2.578 ± 0.354
2.413GluPro: 2.413 ± 0.415
3.072GluGln: 3.072 ± 0.373
3.565GluArg: 3.565 ± 0.378
2.852GluSer: 2.852 ± 0.304
4.059GluThr: 4.059 ± 0.509
4.772GluVal: 4.772 ± 0.675
1.316GluTrp: 1.316 ± 0.237
1.865GluTyr: 1.865 ± 0.309
0.0GluXaa: 0.0 ± 0.0
Phe
2.742PheAla: 2.742 ± 0.39
0.384PheCys: 0.384 ± 0.155
2.304PheAsp: 2.304 ± 0.398
3.126PheGlu: 3.126 ± 0.38
1.316PhePhe: 1.316 ± 0.231
2.907PheGly: 2.907 ± 0.377
0.658PheHis: 0.658 ± 0.17
2.084PheIle: 2.084 ± 0.274
2.029PheLys: 2.029 ± 0.297
3.017PheLeu: 3.017 ± 0.42
0.878PheMet: 0.878 ± 0.22
2.304PheAsn: 2.304 ± 0.263
1.481PhePro: 1.481 ± 0.259
0.987PheGln: 0.987 ± 0.198
2.468PheArg: 2.468 ± 0.346
2.742PheSer: 2.742 ± 0.43
2.139PheThr: 2.139 ± 0.376
2.084PheVal: 2.084 ± 0.292
0.494PheTrp: 0.494 ± 0.167
1.152PheTyr: 1.152 ± 0.321
0.0PheXaa: 0.0 ± 0.0
Gly
7.734GlyAla: 7.734 ± 0.871
0.439GlyCys: 0.439 ± 0.184
4.991GlyAsp: 4.991 ± 0.7
3.894GlyGlu: 3.894 ± 0.478
3.291GlyPhe: 3.291 ± 0.563
6.801GlyGly: 6.801 ± 2.735
1.371GlyHis: 1.371 ± 0.262
4.772GlyIle: 4.772 ± 0.481
4.333GlyLys: 4.333 ± 0.502
6.472GlyLeu: 6.472 ± 0.917
2.688GlyMet: 2.688 ± 0.655
2.523GlyAsn: 2.523 ± 0.39
2.139GlyPro: 2.139 ± 0.515
1.371GlyGln: 1.371 ± 0.3
2.358GlyArg: 2.358 ± 0.345
4.991GlySer: 4.991 ± 0.552
5.595GlyThr: 5.595 ± 0.699
5.375GlyVal: 5.375 ± 0.696
1.591GlyTrp: 1.591 ± 0.364
2.523GlyTyr: 2.523 ± 0.405
0.0GlyXaa: 0.0 ± 0.0
His
1.865HisAla: 1.865 ± 0.396
0.11HisCys: 0.11 ± 0.077
1.042HisAsp: 1.042 ± 0.28
1.481HisGlu: 1.481 ± 0.3
0.548HisPhe: 0.548 ± 0.184
0.932HisGly: 0.932 ± 0.226
0.329HisHis: 0.329 ± 0.127
1.262HisIle: 1.262 ± 0.318
1.042HisLys: 1.042 ± 0.255
1.481HisLeu: 1.481 ± 0.256
0.494HisMet: 0.494 ± 0.163
0.603HisAsn: 0.603 ± 0.209
1.371HisPro: 1.371 ± 0.297
0.878HisGln: 0.878 ± 0.222
1.097HisArg: 1.097 ± 0.249
1.042HisSer: 1.042 ± 0.266
0.548HisThr: 0.548 ± 0.201
1.316HisVal: 1.316 ± 0.276
0.165HisTrp: 0.165 ± 0.121
1.262HisTyr: 1.262 ± 0.277
0.0HisXaa: 0.0 ± 0.0
Ile
5.32IleAla: 5.32 ± 0.877
0.219IleCys: 0.219 ± 0.133
4.498IleAsp: 4.498 ± 0.485
3.291IleGlu: 3.291 ± 0.52
2.029IlePhe: 2.029 ± 0.34
3.565IleGly: 3.565 ± 0.751
1.591IleHis: 1.591 ± 0.338
3.346IleIle: 3.346 ± 0.538
3.675IleLys: 3.675 ± 0.453
4.717IleLeu: 4.717 ± 0.513
1.536IleMet: 1.536 ± 0.277
3.401IleAsn: 3.401 ± 0.333
3.401IlePro: 3.401 ± 0.519
1.975IleGln: 1.975 ± 0.283
2.962IleArg: 2.962 ± 0.445
3.565IleSer: 3.565 ± 0.454
4.059IleThr: 4.059 ± 0.497
4.004IleVal: 4.004 ± 0.482
0.932IleTrp: 0.932 ± 0.255
2.084IleTyr: 2.084 ± 0.33
0.0IleXaa: 0.0 ± 0.0
Lys
6.143LysAla: 6.143 ± 0.867
0.439LysCys: 0.439 ± 0.135
2.633LysAsp: 2.633 ± 0.559
5.265LysGlu: 5.265 ± 0.71
2.688LysPhe: 2.688 ± 0.438
4.388LysGly: 4.388 ± 0.491
1.316LysHis: 1.316 ± 0.325
4.223LysIle: 4.223 ± 0.676
5.595LysLys: 5.595 ± 0.738
5.101LysLeu: 5.101 ± 0.547
2.084LysMet: 2.084 ± 0.311
2.852LysAsn: 2.852 ± 0.396
3.346LysPro: 3.346 ± 0.565
2.358LysGln: 2.358 ± 0.348
3.894LysArg: 3.894 ± 0.662
3.565LysSer: 3.565 ± 0.562
3.455LysThr: 3.455 ± 0.434
3.785LysVal: 3.785 ± 0.426
0.987LysTrp: 0.987 ± 0.249
1.975LysTyr: 1.975 ± 0.317
0.0LysXaa: 0.0 ± 0.0
Leu
6.911LeuAla: 6.911 ± 0.861
0.494LeuCys: 0.494 ± 0.136
4.991LeuAsp: 4.991 ± 0.512
4.882LeuGlu: 4.882 ± 0.48
2.907LeuPhe: 2.907 ± 0.423
5.978LeuGly: 5.978 ± 1.025
1.536LeuHis: 1.536 ± 0.292
5.156LeuIle: 5.156 ± 0.523
5.211LeuLys: 5.211 ± 0.579
4.552LeuLeu: 4.552 ± 0.655
1.865LeuMet: 1.865 ± 0.339
3.565LeuAsn: 3.565 ± 0.408
3.346LeuPro: 3.346 ± 0.412
2.413LeuGln: 2.413 ± 0.34
4.333LeuArg: 4.333 ± 0.402
4.936LeuSer: 4.936 ± 0.62
5.814LeuThr: 5.814 ± 0.717
5.924LeuVal: 5.924 ± 0.671
0.878LeuTrp: 0.878 ± 0.254
2.797LeuTyr: 2.797 ± 0.423
0.0LeuXaa: 0.0 ± 0.0
Met
3.236MetAla: 3.236 ± 0.516
0.219MetCys: 0.219 ± 0.126
1.426MetAsp: 1.426 ± 0.254
1.591MetGlu: 1.591 ± 0.326
0.932MetPhe: 0.932 ± 0.193
1.865MetGly: 1.865 ± 0.401
0.603MetHis: 0.603 ± 0.16
1.481MetIle: 1.481 ± 0.307
2.084MetLys: 2.084 ± 0.385
2.468MetLeu: 2.468 ± 0.392
0.987MetMet: 0.987 ± 0.316
0.768MetAsn: 0.768 ± 0.198
0.987MetPro: 0.987 ± 0.205
0.878MetGln: 0.878 ± 0.251
1.152MetArg: 1.152 ± 0.241
3.346MetSer: 3.346 ± 0.363
1.591MetThr: 1.591 ± 0.326
1.536MetVal: 1.536 ± 0.259
0.384MetTrp: 0.384 ± 0.147
0.548MetTyr: 0.548 ± 0.199
0.0MetXaa: 0.0 ± 0.0
Asn
3.785AsnAla: 3.785 ± 0.397
0.329AsnCys: 0.329 ± 0.137
2.304AsnAsp: 2.304 ± 0.409
2.962AsnGlu: 2.962 ± 0.387
1.426AsnPhe: 1.426 ± 0.213
4.278AsnGly: 4.278 ± 0.656
0.603AsnHis: 0.603 ± 0.162
2.304AsnIle: 2.304 ± 0.417
2.523AsnLys: 2.523 ± 0.344
2.907AsnLeu: 2.907 ± 0.473
1.042AsnMet: 1.042 ± 0.223
1.975AsnAsn: 1.975 ± 0.316
2.688AsnPro: 2.688 ± 0.368
1.645AsnGln: 1.645 ± 0.333
2.578AsnArg: 2.578 ± 0.472
2.907AsnSer: 2.907 ± 0.395
3.072AsnThr: 3.072 ± 0.413
2.523AsnVal: 2.523 ± 0.423
0.878AsnTrp: 0.878 ± 0.233
1.755AsnTyr: 1.755 ± 0.324
0.0AsnXaa: 0.0 ± 0.0
Pro
3.675ProAla: 3.675 ± 0.564
0.274ProCys: 0.274 ± 0.15
2.578ProAsp: 2.578 ± 0.373
3.675ProGlu: 3.675 ± 0.447
1.262ProPhe: 1.262 ± 0.279
3.236ProGly: 3.236 ± 0.378
0.713ProHis: 0.713 ± 0.191
2.139ProIle: 2.139 ± 0.305
3.236ProLys: 3.236 ± 0.537
2.688ProLeu: 2.688 ± 0.346
1.042ProMet: 1.042 ± 0.279
1.92ProAsn: 1.92 ± 0.338
1.975ProPro: 1.975 ± 0.444
1.152ProGln: 1.152 ± 0.296
2.084ProArg: 2.084 ± 0.374
2.962ProSer: 2.962 ± 0.413
3.455ProThr: 3.455 ± 0.55
2.578ProVal: 2.578 ± 0.298
0.494ProTrp: 0.494 ± 0.188
0.878ProTyr: 0.878 ± 0.237
0.0ProXaa: 0.0 ± 0.0
Gln
2.797GlnAla: 2.797 ± 0.468
0.165GlnCys: 0.165 ± 0.101
1.975GlnAsp: 1.975 ± 0.338
1.92GlnGlu: 1.92 ± 0.367
1.207GlnPhe: 1.207 ± 0.272
2.468GlnGly: 2.468 ± 0.273
0.603GlnHis: 0.603 ± 0.211
2.413GlnIle: 2.413 ± 0.317
2.797GlnLys: 2.797 ± 0.421
3.181GlnLeu: 3.181 ± 0.381
1.097GlnMet: 1.097 ± 0.235
1.371GlnAsn: 1.371 ± 0.262
1.371GlnPro: 1.371 ± 0.288
1.7GlnGln: 1.7 ± 0.314
1.92GlnArg: 1.92 ± 0.388
1.426GlnSer: 1.426 ± 0.281
2.139GlnThr: 2.139 ± 0.481
2.084GlnVal: 2.084 ± 0.321
0.768GlnTrp: 0.768 ± 0.207
1.316GlnTyr: 1.316 ± 0.328
0.0GlnXaa: 0.0 ± 0.0
Arg
2.962ArgAla: 2.962 ± 0.516
0.219ArgCys: 0.219 ± 0.1
3.126ArgAsp: 3.126 ± 0.357
3.839ArgGlu: 3.839 ± 0.463
1.316ArgPhe: 1.316 ± 0.231
2.742ArgGly: 2.742 ± 0.316
1.371ArgHis: 1.371 ± 0.28
2.907ArgIle: 2.907 ± 0.339
4.772ArgLys: 4.772 ± 0.623
4.662ArgLeu: 4.662 ± 0.464
1.152ArgMet: 1.152 ± 0.247
2.468ArgAsn: 2.468 ± 0.406
1.92ArgPro: 1.92 ± 0.378
2.358ArgGln: 2.358 ± 0.387
4.004ArgArg: 4.004 ± 0.628
3.072ArgSer: 3.072 ± 0.386
2.907ArgThr: 2.907 ± 0.347
4.552ArgVal: 4.552 ± 0.529
0.768ArgTrp: 0.768 ± 0.227
1.865ArgTyr: 1.865 ± 0.335
0.0ArgXaa: 0.0 ± 0.0
Ser
5.156SerAla: 5.156 ± 0.838
0.165SerCys: 0.165 ± 0.09
3.73SerAsp: 3.73 ± 0.401
3.291SerGlu: 3.291 ± 0.419
2.688SerPhe: 2.688 ± 0.352
5.869SerGly: 5.869 ± 0.766
0.987SerHis: 0.987 ± 0.237
3.73SerIle: 3.73 ± 0.429
4.223SerLys: 4.223 ± 0.418
4.772SerLeu: 4.772 ± 0.439
1.81SerMet: 1.81 ± 0.236
2.962SerAsn: 2.962 ± 0.409
2.578SerPro: 2.578 ± 0.356
1.865SerGln: 1.865 ± 0.289
2.962SerArg: 2.962 ± 0.385
5.375SerSer: 5.375 ± 0.731
3.017SerThr: 3.017 ± 0.389
3.51SerVal: 3.51 ± 0.397
0.987SerTrp: 0.987 ± 0.264
2.304SerTyr: 2.304 ± 0.333
0.0SerXaa: 0.0 ± 0.0
Thr
5.759ThrAla: 5.759 ± 0.669
0.165ThrCys: 0.165 ± 0.099
4.552ThrAsp: 4.552 ± 0.403
3.51ThrGlu: 3.51 ± 0.466
2.523ThrPhe: 2.523 ± 0.368
5.869ThrGly: 5.869 ± 0.752
0.932ThrHis: 0.932 ± 0.271
3.894ThrIle: 3.894 ± 0.429
3.839ThrLys: 3.839 ± 0.429
5.649ThrLeu: 5.649 ± 0.626
1.536ThrMet: 1.536 ± 0.243
2.523ThrAsn: 2.523 ± 0.375
3.455ThrPro: 3.455 ± 0.426
1.591ThrGln: 1.591 ± 0.271
2.578ThrArg: 2.578 ± 0.382
3.291ThrSer: 3.291 ± 0.589
4.333ThrThr: 4.333 ± 0.514
4.223ThrVal: 4.223 ± 0.621
0.987ThrTrp: 0.987 ± 0.27
2.468ThrTyr: 2.468 ± 0.389
0.0ThrXaa: 0.0 ± 0.0
Val
5.814ValAla: 5.814 ± 0.55
0.274ValCys: 0.274 ± 0.118
4.168ValAsp: 4.168 ± 0.635
3.62ValGlu: 3.62 ± 0.518
2.413ValPhe: 2.413 ± 0.42
4.223ValGly: 4.223 ± 0.582
0.932ValHis: 0.932 ± 0.25
4.278ValIle: 4.278 ± 0.506
4.772ValLys: 4.772 ± 0.48
5.32ValLeu: 5.32 ± 0.613
1.536ValMet: 1.536 ± 0.232
3.181ValAsn: 3.181 ± 0.396
2.688ValPro: 2.688 ± 0.338
1.975ValGln: 1.975 ± 0.347
3.73ValArg: 3.73 ± 0.601
4.278ValSer: 4.278 ± 0.523
4.607ValThr: 4.607 ± 0.58
4.662ValVal: 4.662 ± 0.485
0.878ValTrp: 0.878 ± 0.214
2.852ValTyr: 2.852 ± 0.456
0.0ValXaa: 0.0 ± 0.0
Trp
1.207TrpAla: 1.207 ± 0.263
0.055TrpCys: 0.055 ± 0.054
1.262TrpAsp: 1.262 ± 0.283
1.152TrpGlu: 1.152 ± 0.253
0.548TrpPhe: 0.548 ± 0.165
1.371TrpGly: 1.371 ± 0.315
0.274TrpHis: 0.274 ± 0.136
0.932TrpIle: 0.932 ± 0.23
0.658TrpLys: 0.658 ± 0.193
0.987TrpLeu: 0.987 ± 0.21
0.439TrpMet: 0.439 ± 0.158
0.603TrpAsn: 0.603 ± 0.189
0.494TrpPro: 0.494 ± 0.211
0.878TrpGln: 0.878 ± 0.249
1.152TrpArg: 1.152 ± 0.313
1.042TrpSer: 1.042 ± 0.267
1.262TrpThr: 1.262 ± 0.277
0.823TrpVal: 0.823 ± 0.184
0.219TrpTrp: 0.219 ± 0.112
0.494TrpTyr: 0.494 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.017TyrAla: 3.017 ± 0.49
0.384TyrCys: 0.384 ± 0.144
2.523TyrAsp: 2.523 ± 0.56
1.7TyrGlu: 1.7 ± 0.269
1.7TyrPhe: 1.7 ± 0.363
2.742TyrGly: 2.742 ± 0.37
0.768TyrHis: 0.768 ± 0.219
1.81TyrIle: 1.81 ± 0.311
2.468TyrLys: 2.468 ± 0.406
1.865TyrLeu: 1.865 ± 0.307
0.823TyrMet: 0.823 ± 0.221
1.536TyrAsn: 1.536 ± 0.198
1.262TyrPro: 1.262 ± 0.302
1.371TyrGln: 1.371 ± 0.262
2.029TyrArg: 2.029 ± 0.287
1.865TyrSer: 1.865 ± 0.302
2.084TyrThr: 2.084 ± 0.382
2.358TyrVal: 2.358 ± 0.358
0.329TyrTrp: 0.329 ± 0.182
1.097TyrTyr: 1.097 ± 0.331
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (18233 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski