Amino acid dipepetide frequency for Daphnia magna

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.716AlaAla: 6.716 ± 0.056	1.146AlaCys: 1.146 ± 0.016	3.45AlaAsp: 3.45 ± 0.022	4.106AlaGlu: 4.106 ± 0.029	2.553AlaPhe: 2.553 ± 0.018	4.051AlaGly: 4.051 ± 0.034	1.467AlaHis: 1.467 ± 0.014	3.777AlaIle: 3.777 ± 0.022	3.593AlaLys: 3.593 ± 0.025	6.098AlaLeu: 6.098 ± 0.031	1.606AlaMet: 1.606 ± 0.013	2.994AlaAsn: 2.994 ± 0.018	3.499AlaPro: 3.499 ± 0.047	2.538AlaGln: 2.538 ± 0.023	3.321AlaArg: 3.321 ± 0.024	5.877AlaSer: 5.877 ± 0.031	4.325AlaThr: 4.325 ± 0.024	4.962AlaVal: 4.962 ± 0.026	0.684AlaTrp: 0.684 ± 0.009	1.775AlaTyr: 1.775 ± 0.018	0.001AlaXaa: 0.001 ± 0.0
Cys	1.064CysAla: 1.064 ± 0.016	0.554CysCys: 0.554 ± 0.009	1.036CysAsp: 1.036 ± 0.018	0.988CysGlu: 0.988 ± 0.013	0.89CysPhe: 0.89 ± 0.013	1.284CysGly: 1.284 ± 0.02	0.591CysHis: 0.591 ± 0.01	0.992CysIle: 0.992 ± 0.013	0.958CysLys: 0.958 ± 0.012	1.998CysLeu: 1.998 ± 0.02	0.388CysMet: 0.388 ± 0.007	0.891CysAsn: 0.891 ± 0.013	1.125CysPro: 1.125 ± 0.023	0.911CysGln: 0.911 ± 0.016	1.249CysArg: 1.249 ± 0.024	1.713CysSer: 1.713 ± 0.019	0.984CysThr: 0.984 ± 0.015	1.311CysVal: 1.311 ± 0.022	0.304CysTrp: 0.304 ± 0.006	0.565CysTyr: 0.565 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	3.277AspAla: 3.277 ± 0.021	1.052AspCys: 1.052 ± 0.018	3.471AspAsp: 3.471 ± 0.027	3.86AspGlu: 3.86 ± 0.029	2.225AspPhe: 2.225 ± 0.017	3.458AspGly: 3.458 ± 0.024	1.224AspHis: 1.224 ± 0.012	2.837AspIle: 2.837 ± 0.017	2.434AspLys: 2.434 ± 0.018	4.666AspLeu: 4.666 ± 0.027	1.07AspMet: 1.07 ± 0.011	2.081AspAsn: 2.081 ± 0.016	2.565AspPro: 2.565 ± 0.025	1.941AspGln: 1.941 ± 0.014	2.626AspArg: 2.626 ± 0.022	4.095AspSer: 4.095 ± 0.027	2.366AspThr: 2.366 ± 0.018	3.651AspVal: 3.651 ± 0.025	0.766AspTrp: 0.766 ± 0.01	1.639AspTyr: 1.639 ± 0.014	0.001AspXaa: 0.001 ± 0.0
Glu	4.159GluAla: 4.159 ± 0.029	1.05GluCys: 1.05 ± 0.022	3.764GluAsp: 3.764 ± 0.027	5.824GluGlu: 5.824 ± 0.054	2.118GluPhe: 2.118 ± 0.019	2.87GluGly: 2.87 ± 0.019	1.294GluHis: 1.294 ± 0.013	3.499GluIle: 3.499 ± 0.026	4.308GluLys: 4.308 ± 0.031	5.414GluLeu: 5.414 ± 0.034	1.63GluMet: 1.63 ± 0.015	3.157GluAsn: 3.157 ± 0.023	2.506GluPro: 2.506 ± 0.027	2.521GluGln: 2.521 ± 0.022	3.625GluArg: 3.625 ± 0.031	4.563GluSer: 4.563 ± 0.035	3.729GluThr: 3.729 ± 0.03	3.423GluVal: 3.423 ± 0.025	0.742GluTrp: 0.742 ± 0.01	1.514GluTyr: 1.514 ± 0.015	0.001GluXaa: 0.001 ± 0.0
Phe	2.416PheAla: 2.416 ± 0.019	0.921PheCys: 0.921 ± 0.012	2.081PheAsp: 2.081 ± 0.018	2.064PheGlu: 2.064 ± 0.017	2.065PhePhe: 2.065 ± 0.02	2.446PheGly: 2.446 ± 0.021	1.16PheHis: 1.16 ± 0.012	2.2PheIle: 2.2 ± 0.018	1.983PheLys: 1.983 ± 0.018	4.048PheLeu: 4.048 ± 0.027	0.828PheMet: 0.828 ± 0.01	1.813PheAsn: 1.813 ± 0.015	1.936PhePro: 1.936 ± 0.014	1.739PheGln: 1.739 ± 0.015	2.223PheArg: 2.223 ± 0.017	3.416PheSer: 3.416 ± 0.021	2.184PheThr: 2.184 ± 0.016	2.698PheVal: 2.698 ± 0.02	0.503PheTrp: 0.503 ± 0.008	1.33PheTyr: 1.33 ± 0.013	0.001PheXaa: 0.001 ± 0.0
Gly	3.551GlyAla: 3.551 ± 0.03	1.1GlyCys: 1.1 ± 0.018	2.794GlyAsp: 2.794 ± 0.021	3.028GlyGlu: 3.028 ± 0.023	2.355GlyPhe: 2.355 ± 0.021	4.732GlyGly: 4.732 ± 0.058	1.606GlyHis: 1.606 ± 0.014	3.016GlyIle: 3.016 ± 0.021	3.333GlyLys: 3.333 ± 0.026	4.915GlyLeu: 4.915 ± 0.031	1.264GlyMet: 1.264 ± 0.014	2.653GlyAsn: 2.653 ± 0.022	2.696GlyPro: 2.696 ± 0.043	2.372GlyGln: 2.372 ± 0.02	3.529GlyArg: 3.529 ± 0.023	5.073GlySer: 5.073 ± 0.034	3.192GlyThr: 3.192 ± 0.023	3.454GlyVal: 3.454 ± 0.023	0.763GlyTrp: 0.763 ± 0.011	1.904GlyTyr: 1.904 ± 0.022	0.002GlyXaa: 0.002 ± 0.0
His	1.36HisAla: 1.36 ± 0.013	0.581HisCys: 0.581 ± 0.009	1.048HisAsp: 1.048 ± 0.012	1.213HisGlu: 1.213 ± 0.013	1.209HisPhe: 1.209 ± 0.01	1.485HisGly: 1.485 ± 0.014	1.378HisHis: 1.378 ± 0.029	1.253HisIle: 1.253 ± 0.012	1.073HisLys: 1.073 ± 0.011	2.725HisLeu: 2.725 ± 0.02	0.509HisMet: 0.509 ± 0.008	1.022HisAsn: 1.022 ± 0.011	1.674HisPro: 1.674 ± 0.016	1.5HisGln: 1.5 ± 0.017	1.607HisArg: 1.607 ± 0.017	2.15HisSer: 2.15 ± 0.018	1.221HisThr: 1.221 ± 0.014	1.604HisVal: 1.604 ± 0.014	0.348HisTrp: 0.348 ± 0.007	0.821HisTyr: 0.821 ± 0.009	0.0HisXaa: 0.0 ± 0.0
Ile	3.462IleAla: 3.462 ± 0.021	1.121IleCys: 1.121 ± 0.012	2.931IleAsp: 2.931 ± 0.018	3.232IleGlu: 3.232 ± 0.022	2.231IlePhe: 2.231 ± 0.02	2.866IleGly: 2.866 ± 0.02	1.429IleHis: 1.429 ± 0.014	2.842IleIle: 2.842 ± 0.022	2.859IleLys: 2.859 ± 0.02	4.827IleLeu: 4.827 ± 0.03	1.057IleMet: 1.057 ± 0.013	2.401IleAsn: 2.401 ± 0.018	2.953IlePro: 2.953 ± 0.021	2.406IleGln: 2.406 ± 0.019	3.034IleArg: 3.034 ± 0.018	4.211IleSer: 4.211 ± 0.027	2.892IleThr: 2.892 ± 0.018	3.504IleVal: 3.504 ± 0.025	0.582IleTrp: 0.582 ± 0.009	1.515IleTyr: 1.515 ± 0.015	0.001IleXaa: 0.001 ± 0.0
Lys	3.495LysAla: 3.495 ± 0.025	1.061LysCys: 1.061 ± 0.013	2.75LysAsp: 2.75 ± 0.021	4.141LysGlu: 4.141 ± 0.036	2.018LysPhe: 2.018 ± 0.019	2.675LysGly: 2.675 ± 0.025	1.243LysHis: 1.243 ± 0.012	3.191LysIle: 3.191 ± 0.026	4.695LysLys: 4.695 ± 0.041	5.111LysLeu: 5.111 ± 0.025	1.508LysMet: 1.508 ± 0.015	2.716LysAsn: 2.716 ± 0.02	2.843LysPro: 2.843 ± 0.025	2.351LysGln: 2.351 ± 0.019	3.49LysArg: 3.49 ± 0.025	4.241LysSer: 4.241 ± 0.025	3.4LysThr: 3.4 ± 0.022	3.041LysVal: 3.041 ± 0.021	0.735LysTrp: 0.735 ± 0.009	1.526LysTyr: 1.526 ± 0.016	0.001LysXaa: 0.001 ± 0.0
Leu	6.628LeuAla: 6.628 ± 0.033	1.896LeuCys: 1.896 ± 0.017	4.707LeuAsp: 4.707 ± 0.028	5.68LeuGlu: 5.68 ± 0.037	3.674LeuPhe: 3.674 ± 0.027	4.657LeuGly: 4.657 ± 0.025	2.463LeuHis: 2.463 ± 0.019	4.586LeuIle: 4.586 ± 0.025	5.52LeuLys: 5.52 ± 0.03	9.446LeuLeu: 9.446 ± 0.047	2.146LeuMet: 2.146 ± 0.016	4.142LeuAsn: 4.142 ± 0.026	5.293LeuPro: 5.293 ± 0.029	4.312LeuGln: 4.312 ± 0.026	5.334LeuArg: 5.334 ± 0.027	7.54LeuSer: 7.54 ± 0.034	5.233LeuThr: 5.233 ± 0.027	5.729LeuVal: 5.729 ± 0.032	1.0LeuTrp: 1.0 ± 0.011	2.371LeuTyr: 2.371 ± 0.019	0.001LeuXaa: 0.001 ± 0.0
Met	2.079MetAla: 2.079 ± 0.015	0.377MetCys: 0.377 ± 0.006	1.343MetAsp: 1.343 ± 0.01	1.738MetGlu: 1.738 ± 0.016	0.786MetPhe: 0.786 ± 0.01	1.252MetGly: 1.252 ± 0.017	0.442MetHis: 0.442 ± 0.008	1.039MetIle: 1.039 ± 0.01	1.461MetLys: 1.461 ± 0.015	1.94MetLeu: 1.94 ± 0.016	0.672MetMet: 0.672 ± 0.011	1.027MetAsn: 1.027 ± 0.011	1.07MetPro: 1.07 ± 0.013	0.897MetGln: 0.897 ± 0.011	1.128MetArg: 1.128 ± 0.011	1.766MetSer: 1.766 ± 0.016	1.335MetThr: 1.335 ± 0.012	1.362MetVal: 1.362 ± 0.012	0.238MetTrp: 0.238 ± 0.005	0.508MetTyr: 0.508 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	2.906AsnAla: 2.906 ± 0.021	0.946AsnCys: 0.946 ± 0.013	2.233AsnAsp: 2.233 ± 0.019	2.758AsnGlu: 2.758 ± 0.019	1.893AsnPhe: 1.893 ± 0.014	3.135AsnGly: 3.135 ± 0.024	1.224AsnHis: 1.224 ± 0.013	2.398AsnIle: 2.398 ± 0.017	2.474AsnLys: 2.474 ± 0.021	4.059AsnLeu: 4.059 ± 0.025	0.981AsnMet: 0.981 ± 0.012	2.53AsnAsn: 2.53 ± 0.027	2.44AsnPro: 2.44 ± 0.019	2.069AsnGln: 2.069 ± 0.018	2.579AsnArg: 2.579 ± 0.019	3.875AsnSer: 3.875 ± 0.026	2.308AsnThr: 2.308 ± 0.016	2.909AsnVal: 2.909 ± 0.019	0.583AsnTrp: 0.583 ± 0.008	1.397AsnTyr: 1.397 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	4.106ProAla: 4.106 ± 0.035	0.887ProCys: 0.887 ± 0.021	2.553ProAsp: 2.553 ± 0.02	3.132ProGlu: 3.132 ± 0.028	2.132ProPhe: 2.132 ± 0.019	2.992ProGly: 2.992 ± 0.053	1.229ProHis: 1.229 ± 0.015	2.756ProIle: 2.756 ± 0.019	2.613ProLys: 2.613 ± 0.02	4.583ProLeu: 4.583 ± 0.029	1.055ProMet: 1.055 ± 0.012	2.292ProAsn: 2.292 ± 0.018	4.335ProPro: 4.335 ± 0.053	2.096ProGln: 2.096 ± 0.019	2.555ProArg: 2.555 ± 0.021	5.507ProSer: 5.507 ± 0.04	3.696ProThr: 3.696 ± 0.031	3.966ProVal: 3.966 ± 0.03	0.582ProTrp: 0.582 ± 0.008	1.426ProTyr: 1.426 ± 0.016	0.001ProXaa: 0.001 ± 0.0
Gln	2.722GlnAla: 2.722 ± 0.025	0.834GlnCys: 0.834 ± 0.017	1.788GlnAsp: 1.788 ± 0.015	2.648GlnGlu: 2.648 ± 0.026	1.554GlnPhe: 1.554 ± 0.014	2.001GlnGly: 2.001 ± 0.019	1.406GlnHis: 1.406 ± 0.018	2.122GlnIle: 2.122 ± 0.017	2.26GlnLys: 2.26 ± 0.019	4.578GlnLeu: 4.578 ± 0.032	1.087GlnMet: 1.087 ± 0.012	1.8GlnAsn: 1.8 ± 0.015	2.612GlnPro: 2.612 ± 0.022	4.126GlnGln: 4.126 ± 0.079	2.755GlnArg: 2.755 ± 0.019	3.374GlnSer: 3.374 ± 0.023	2.438GlnThr: 2.438 ± 0.019	2.549GlnVal: 2.549 ± 0.02	0.546GlnTrp: 0.546 ± 0.008	1.117GlnTyr: 1.117 ± 0.014	0.001GlnXaa: 0.001 ± 0.0
Arg	3.175ArgAla: 3.175 ± 0.023	1.147ArgCys: 1.147 ± 0.017	2.703ArgAsp: 2.703 ± 0.021	3.287ArgGlu: 3.287 ± 0.03	2.277ArgPhe: 2.277 ± 0.016	2.884ArgGly: 2.884 ± 0.025	1.667ArgHis: 1.667 ± 0.015	2.974ArgIle: 2.974 ± 0.02	3.658ArgLys: 3.658 ± 0.024	5.545ArgLeu: 5.545 ± 0.03	1.34ArgMet: 1.34 ± 0.012	2.739ArgAsn: 2.739 ± 0.017	3.144ArgPro: 3.144 ± 0.029	2.747ArgGln: 2.747 ± 0.017	4.41ArgArg: 4.41 ± 0.034	4.61ArgSer: 4.61 ± 0.031	2.92ArgThr: 2.92 ± 0.02	3.115ArgVal: 3.115 ± 0.021	0.775ArgTrp: 0.775 ± 0.009	1.627ArgTyr: 1.627 ± 0.015	0.001ArgXaa: 0.001 ± 0.0
Ser	5.774SerAla: 5.774 ± 0.035	1.691SerCys: 1.691 ± 0.022	4.251SerAsp: 4.251 ± 0.026	4.329SerGlu: 4.329 ± 0.03	3.464SerPhe: 3.464 ± 0.023	5.186SerGly: 5.186 ± 0.031	2.078SerHis: 2.078 ± 0.018	4.174SerIle: 4.174 ± 0.025	4.257SerLys: 4.257 ± 0.025	7.634SerLeu: 7.634 ± 0.034	1.732SerMet: 1.732 ± 0.014	3.952SerAsn: 3.952 ± 0.029	4.937SerPro: 4.937 ± 0.039	3.394SerGln: 3.394 ± 0.021	4.704SerArg: 4.704 ± 0.031	10.527SerSer: 10.527 ± 0.079	5.625SerThr: 5.625 ± 0.037	5.279SerVal: 5.279 ± 0.028	0.967SerTrp: 0.967 ± 0.012	2.369SerTyr: 2.369 ± 0.021	0.001SerXaa: 0.001 ± 0.0
Thr	4.38ThrAla: 4.38 ± 0.025	1.188ThrCys: 1.188 ± 0.018	2.799ThrAsp: 2.799 ± 0.021	3.437ThrGlu: 3.437 ± 0.03	2.222ThrPhe: 2.222 ± 0.017	3.361ThrGly: 3.361 ± 0.024	1.255ThrHis: 1.255 ± 0.012	3.121ThrIle: 3.121 ± 0.022	3.082ThrLys: 3.082 ± 0.02	5.185ThrLeu: 5.185 ± 0.023	1.199ThrMet: 1.199 ± 0.011	2.617ThrAsn: 2.617 ± 0.018	3.566ThrPro: 3.566 ± 0.026	2.113ThrGln: 2.113 ± 0.017	2.854ThrArg: 2.854 ± 0.02	5.546ThrSer: 5.546 ± 0.034	4.968ThrThr: 4.968 ± 0.072	3.981ThrVal: 3.981 ± 0.026	0.672ThrTrp: 0.672 ± 0.01	1.507ThrTyr: 1.507 ± 0.016	0.001ThrXaa: 0.001 ± 0.0
Val	4.838ValAla: 4.838 ± 0.029	1.369ValCys: 1.369 ± 0.022	3.51ValAsp: 3.51 ± 0.021	4.064ValGlu: 4.064 ± 0.034	2.584ValPhe: 2.584 ± 0.018	3.405ValGly: 3.405 ± 0.022	1.552ValHis: 1.552 ± 0.015	3.42ValIle: 3.42 ± 0.022	3.48ValLys: 3.48 ± 0.024	5.676ValLeu: 5.676 ± 0.028	1.443ValMet: 1.443 ± 0.014	2.879ValAsn: 2.879 ± 0.019	3.265ValPro: 3.265 ± 0.024	2.574ValGln: 2.574 ± 0.022	3.271ValArg: 3.271 ± 0.022	5.078ValSer: 5.078 ± 0.028	4.008ValThr: 4.008 ± 0.026	4.653ValVal: 4.653 ± 0.027	0.743ValTrp: 0.743 ± 0.011	1.797ValTyr: 1.797 ± 0.018	0.001ValXaa: 0.001 ± 0.0
Trp	0.706TrpAla: 0.706 ± 0.009	0.23TrpCys: 0.23 ± 0.006	0.621TrpAsp: 0.621 ± 0.009	0.643TrpGlu: 0.643 ± 0.008	0.483TrpPhe: 0.483 ± 0.008	0.611TrpGly: 0.611 ± 0.009	0.281TrpHis: 0.281 ± 0.006	0.79TrpIle: 0.79 ± 0.011	0.843TrpLys: 0.843 ± 0.01	1.239TrpLeu: 1.239 ± 0.013	0.362TrpMet: 0.362 ± 0.006	0.723TrpAsn: 0.723 ± 0.009	0.541TrpPro: 0.541 ± 0.008	0.496TrpGln: 0.496 ± 0.008	0.754TrpArg: 0.754 ± 0.009	0.909TrpSer: 0.909 ± 0.011	0.756TrpThr: 0.756 ± 0.01	0.633TrpVal: 0.633 ± 0.009	0.203TrpTrp: 0.203 ± 0.005	0.328TrpTyr: 0.328 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.72TyrAla: 1.72 ± 0.016	0.646TyrCys: 0.646 ± 0.01	1.469TyrAsp: 1.469 ± 0.013	1.536TyrGlu: 1.536 ± 0.015	1.319TyrPhe: 1.319 ± 0.013	1.982TyrGly: 1.982 ± 0.025	0.789TyrHis: 0.789 ± 0.01	1.367TyrIle: 1.367 ± 0.015	1.345TyrLys: 1.345 ± 0.014	2.671TyrLeu: 2.671 ± 0.019	0.581TyrMet: 0.581 ± 0.008	1.267TyrAsn: 1.267 ± 0.015	1.496TyrPro: 1.496 ± 0.017	1.216TyrGln: 1.216 ± 0.013	1.698TyrArg: 1.698 ± 0.015	2.254TyrSer: 2.254 ± 0.02	1.475TyrThr: 1.475 ± 0.021	1.788TyrVal: 1.788 ± 0.016	0.415TyrTrp: 0.415 ± 0.008	1.096TyrTyr: 1.096 ± 0.019	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.002XaaLys: 0.002 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.001XaaSer: 0.001 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	1.536XaaXaa: 1.536 ± 0.156

Statistics based on 26600 proteins (9136891 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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