Poophage SC_4_H6H8_2017

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H0X6Q9|A0A6H0X6Q9_9VIRU Major capsid protein OS=Poophage SC_4_H6H8_2017 OX=2723248 PE=4 SV=1
MM1 pKa = 7.71FYY3 pKa = 10.55PRR5 pKa = 11.84DD6 pKa = 3.42RR7 pKa = 11.84KK8 pKa = 10.38ISVDD12 pKa = 3.47RR13 pKa = 11.84DD14 pKa = 3.02EE15 pKa = 4.93LTWNRR20 pKa = 11.84SDD22 pKa = 4.58LFIEE26 pKa = 4.47ILRR29 pKa = 11.84SDD31 pKa = 4.75LFNKK35 pKa = 8.29GTEE38 pKa = 3.81AATPDD43 pKa = 4.0FNKK46 pKa = 10.02LLQMFPQNIDD56 pKa = 3.43VNVPAYY62 pKa = 9.43PYY64 pKa = 10.45DD65 pKa = 3.69VQEE68 pKa = 5.4PKK70 pKa = 11.04VNWNNGEE77 pKa = 4.17GTDD80 pKa = 3.39TSTPSKK86 pKa = 10.89VYY88 pKa = 9.9FAATLNVPFLAAHH101 pKa = 6.71PMAVCPSSPDD111 pKa = 3.03RR112 pKa = 11.84FSRR115 pKa = 11.84LMPPGDD121 pKa = 4.01SNSDD125 pKa = 2.95VDD127 pKa = 4.08FTGVKK132 pKa = 9.41TIPQLAVATRR142 pKa = 11.84LQEE145 pKa = 4.14YY146 pKa = 9.86KK147 pKa = 10.89DD148 pKa = 4.36LIGASGSRR156 pKa = 11.84YY157 pKa = 10.07SDD159 pKa = 2.73WLYY162 pKa = 10.59TFFASKK168 pKa = 9.86IEE170 pKa = 4.19HH171 pKa = 6.01VDD173 pKa = 3.36RR174 pKa = 11.84PKK176 pKa = 11.08LLFSSSVMVNSQVVMNQAGQSGFAGGEE203 pKa = 3.85AAALGQMGGSIAFNSVLGRR222 pKa = 11.84EE223 pKa = 3.68QTYY226 pKa = 8.84YY227 pKa = 10.69FKK229 pKa = 10.98EE230 pKa = 3.83PGYY233 pKa = 10.21IFDD236 pKa = 4.48MLTIRR241 pKa = 11.84PVYY244 pKa = 8.65FWTGIRR250 pKa = 11.84PDD252 pKa = 3.5YY253 pKa = 10.95LEE255 pKa = 4.15YY256 pKa = 10.7RR257 pKa = 11.84GPDD260 pKa = 3.67YY261 pKa = 11.0FNPIYY266 pKa = 10.87NDD268 pKa = 2.44IGYY271 pKa = 9.79QDD273 pKa = 3.41VPFWRR278 pKa = 11.84IGYY281 pKa = 7.85GWKK284 pKa = 10.4GGSPSQSMTVAKK296 pKa = 9.54EE297 pKa = 3.45PCYY300 pKa = 10.87NEE302 pKa = 3.93FRR304 pKa = 11.84SSYY307 pKa = 11.26DD308 pKa = 3.25EE309 pKa = 4.04VLGTLQSTLTPKK321 pKa = 9.87ATVPLQSYY329 pKa = 7.57WVQQRR334 pKa = 11.84DD335 pKa = 4.13FYY337 pKa = 11.56SIGLSSNYY345 pKa = 10.12NEE347 pKa = 5.05ISPSMLFTNLSTVNNPFASDD367 pKa = 3.64LEE369 pKa = 4.92DD370 pKa = 3.32NFFVNMSYY378 pKa = 10.9KK379 pKa = 10.43VVVKK383 pKa = 10.64SLVNKK388 pKa = 10.29SFATRR393 pKa = 11.84LSSRR397 pKa = 3.61

Molecular weight: 44.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H0X6Q9|A0A6H0X6Q9_9VIRU Major capsid protein OS=Poophage SC_4_H6H8_2017 OX=2723248 PE=4 SV=1
MM1 pKa = 7.63CLAPIWIRR9 pKa = 11.84NRR11 pKa = 11.84AYY13 pKa = 10.37SSRR16 pKa = 11.84TIGLTDD22 pKa = 3.81RR23 pKa = 11.84KK24 pKa = 9.77VLLMNRR30 pKa = 11.84PWDD33 pKa = 3.75YY34 pKa = 8.37FTQRR38 pKa = 11.84IMVPCGRR45 pKa = 11.84CEE47 pKa = 3.64EE48 pKa = 4.66CLRR51 pKa = 11.84QQRR54 pKa = 11.84NDD56 pKa = 2.43WYY58 pKa = 10.3IRR60 pKa = 11.84LEE62 pKa = 4.44RR63 pKa = 11.84EE64 pKa = 3.68TKK66 pKa = 8.74YY67 pKa = 10.74QKK69 pKa = 10.45SLYY72 pKa = 8.7RR73 pKa = 11.84NSVFVTITIAPEE85 pKa = 4.25YY86 pKa = 10.13YY87 pKa = 10.48DD88 pKa = 4.65SALQNPSSFIRR99 pKa = 11.84LWFEE103 pKa = 4.13RR104 pKa = 11.84IRR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84FGHH111 pKa = 5.88SIKK114 pKa = 10.55HH115 pKa = 5.69AVFQEE120 pKa = 4.44FGVHH124 pKa = 6.6PEE126 pKa = 4.16LGNEE130 pKa = 4.02PRR132 pKa = 11.84LHH134 pKa = 5.27FHH136 pKa = 6.16GVLWDD141 pKa = 3.78VPHH144 pKa = 6.99SYY146 pKa = 10.8NAIRR150 pKa = 11.84EE151 pKa = 4.29AVKK154 pKa = 10.73DD155 pKa = 4.02LGFVWIASITDD166 pKa = 2.8KK167 pKa = 10.78RR168 pKa = 11.84LRR170 pKa = 11.84YY171 pKa = 8.12VVKK174 pKa = 10.49YY175 pKa = 9.69VGKK178 pKa = 9.73SVYY181 pKa = 9.29MDD183 pKa = 3.17EE184 pKa = 5.22RR185 pKa = 11.84SADD188 pKa = 3.64FAKK191 pKa = 10.58SLPITVGKK199 pKa = 10.67LNTNLYY205 pKa = 11.01DD206 pKa = 3.75FLQNSKK212 pKa = 9.29YY213 pKa = 9.52RR214 pKa = 11.84RR215 pKa = 11.84KK216 pKa = 10.03FISAGVGDD224 pKa = 3.9YY225 pKa = 11.24LGDD228 pKa = 4.28FKK230 pKa = 11.68APGAASGLWSYY241 pKa = 11.63TDD243 pKa = 3.86FKK245 pKa = 10.7TGVVYY250 pKa = 10.05RR251 pKa = 11.84YY252 pKa = 9.64RR253 pKa = 11.84IPRR256 pKa = 11.84YY257 pKa = 7.48YY258 pKa = 10.73DD259 pKa = 3.09KK260 pKa = 11.34YY261 pKa = 10.65LSQDD265 pKa = 2.77ALVFRR270 pKa = 11.84KK271 pKa = 9.92ISTAWTYY278 pKa = 11.7ASAFGGSLALGFLRR292 pKa = 11.84EE293 pKa = 3.85VAEE296 pKa = 4.16RR297 pKa = 11.84VLRR300 pKa = 11.84PSDD303 pKa = 3.52FSRR306 pKa = 11.84VVKK309 pKa = 10.65GGFSRR314 pKa = 11.84LVKK317 pKa = 10.46LRR319 pKa = 11.84EE320 pKa = 3.91FLSKK324 pKa = 11.05VKK326 pKa = 10.43DD327 pKa = 3.6RR328 pKa = 11.84PSFLAVTSDD337 pKa = 5.01VIDD340 pKa = 4.14FWVDD344 pKa = 2.93CFGIDD349 pKa = 3.84PSNPFFNKK357 pKa = 9.48IVYY360 pKa = 9.16GG361 pKa = 3.88

Molecular weight: 42.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

758

361

397

379.0

43.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.673 ± 0.096

0.923 ± 0.306

6.069 ± 0.166

3.958 ± 0.053

6.728 ± 0.13

6.201 ± 0.07

1.055 ± 0.402

4.749 ± 0.524

4.881 ± 0.62

7.784 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.979 ± 0.577

4.749 ± 0.944

5.541 ± 0.918

3.166 ± 0.629

7.124 ± 1.703

8.971 ± 0.804

4.749 ± 0.577

7.652 ± 0.253

2.111 ± 0.253

5.937 ± 0.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski