Amino acid dipepetide frequency for Arthrobacter phage Edmundo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.602AlaAla: 17.602 ± 1.599
0.803AlaCys: 0.803 ± 0.242
6.581AlaAsp: 6.581 ± 0.626
8.132AlaGlu: 8.132 ± 0.812
2.996AlaPhe: 2.996 ± 0.486
10.7AlaGly: 10.7 ± 1.177
1.926AlaHis: 1.926 ± 0.306
6.046AlaIle: 6.046 ± 0.834
5.136AlaLys: 5.136 ± 0.564
9.042AlaLeu: 9.042 ± 0.746
3.264AlaMet: 3.264 ± 0.497
2.461AlaAsn: 2.461 ± 0.285
6.367AlaPro: 6.367 ± 0.868
3.906AlaGln: 3.906 ± 0.439
6.795AlaArg: 6.795 ± 0.639
5.778AlaSer: 5.778 ± 0.656
7.276AlaThr: 7.276 ± 0.795
9.095AlaVal: 9.095 ± 0.919
1.926AlaTrp: 1.926 ± 0.315
2.194AlaTyr: 2.194 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
0.589CysAla: 0.589 ± 0.196
0.054CysCys: 0.054 ± 0.051
0.482CysAsp: 0.482 ± 0.167
0.482CysGlu: 0.482 ± 0.159
0.161CysPhe: 0.161 ± 0.095
0.803CysGly: 0.803 ± 0.2
0.268CysHis: 0.268 ± 0.114
0.214CysIle: 0.214 ± 0.093
0.482CysLys: 0.482 ± 0.153
0.482CysLeu: 0.482 ± 0.173
0.161CysMet: 0.161 ± 0.091
0.054CysAsn: 0.054 ± 0.06
1.017CysPro: 1.017 ± 0.201
0.428CysGln: 0.428 ± 0.173
0.428CysArg: 0.428 ± 0.173
0.214CysSer: 0.214 ± 0.104
0.589CysThr: 0.589 ± 0.191
0.375CysVal: 0.375 ± 0.143
0.268CysTrp: 0.268 ± 0.103
0.214CysTyr: 0.214 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
7.223AspAla: 7.223 ± 0.613
0.482AspCys: 0.482 ± 0.2
2.782AspAsp: 2.782 ± 0.453
4.387AspGlu: 4.387 ± 0.585
1.177AspPhe: 1.177 ± 0.235
4.762AspGly: 4.762 ± 0.582
1.605AspHis: 1.605 ± 0.287
2.194AspIle: 2.194 ± 0.326
1.659AspLys: 1.659 ± 0.299
4.762AspLeu: 4.762 ± 0.542
1.552AspMet: 1.552 ± 0.319
1.766AspAsn: 1.766 ± 0.313
4.334AspPro: 4.334 ± 0.447
1.445AspGln: 1.445 ± 0.218
3.638AspArg: 3.638 ± 0.462
2.515AspSer: 2.515 ± 0.383
3.157AspThr: 3.157 ± 0.417
4.227AspVal: 4.227 ± 0.511
1.124AspTrp: 1.124 ± 0.208
1.445AspTyr: 1.445 ± 0.329
0.0AspXaa: 0.0 ± 0.0
Glu
8.614GluAla: 8.614 ± 0.733
0.428GluCys: 0.428 ± 0.193
3.371GluAsp: 3.371 ± 0.457
2.461GluGlu: 2.461 ± 0.313
1.712GluPhe: 1.712 ± 0.291
4.227GluGly: 4.227 ± 0.564
0.963GluHis: 0.963 ± 0.239
2.996GluIle: 2.996 ± 0.415
2.408GluLys: 2.408 ± 0.399
5.029GluLeu: 5.029 ± 0.597
1.605GluMet: 1.605 ± 0.376
1.552GluAsn: 1.552 ± 0.307
3.317GluPro: 3.317 ± 0.477
2.515GluGln: 2.515 ± 0.342
4.387GluArg: 4.387 ± 0.692
3.05GluSer: 3.05 ± 0.399
3.745GluThr: 3.745 ± 0.452
5.136GluVal: 5.136 ± 0.57
1.231GluTrp: 1.231 ± 0.298
1.98GluTyr: 1.98 ± 0.34
0.0GluXaa: 0.0 ± 0.0
Phe
2.14PheAla: 2.14 ± 0.327
0.107PheCys: 0.107 ± 0.067
1.659PheAsp: 1.659 ± 0.334
1.124PheGlu: 1.124 ± 0.236
0.321PhePhe: 0.321 ± 0.122
1.766PheGly: 1.766 ± 0.336
0.589PheHis: 0.589 ± 0.182
1.391PheIle: 1.391 ± 0.366
1.391PheLys: 1.391 ± 0.276
1.98PheLeu: 1.98 ± 0.331
0.803PheMet: 0.803 ± 0.229
1.231PheAsn: 1.231 ± 0.456
1.07PhePro: 1.07 ± 0.221
0.589PheGln: 0.589 ± 0.161
1.926PheArg: 1.926 ± 0.305
1.712PheSer: 1.712 ± 0.318
2.943PheThr: 2.943 ± 0.562
1.659PheVal: 1.659 ± 0.291
0.375PheTrp: 0.375 ± 0.131
0.803PheTyr: 0.803 ± 0.209
0.0PheXaa: 0.0 ± 0.0
Gly
8.239GlyAla: 8.239 ± 1.022
0.535GlyCys: 0.535 ± 0.157
5.564GlyAsp: 5.564 ± 0.561
4.441GlyGlu: 4.441 ± 0.616
2.729GlyPhe: 2.729 ± 0.57
8.025GlyGly: 8.025 ± 1.353
1.873GlyHis: 1.873 ± 0.387
3.799GlyIle: 3.799 ± 0.582
3.317GlyLys: 3.317 ± 0.484
5.671GlyLeu: 5.671 ± 0.639
2.087GlyMet: 2.087 ± 0.326
2.461GlyAsn: 2.461 ± 0.388
2.782GlyPro: 2.782 ± 0.43
3.906GlyGln: 3.906 ± 0.608
5.136GlyArg: 5.136 ± 0.504
5.243GlySer: 5.243 ± 0.69
6.313GlyThr: 6.313 ± 0.588
7.651GlyVal: 7.651 ± 0.795
2.408GlyTrp: 2.408 ± 0.394
2.675GlyTyr: 2.675 ± 0.412
0.0GlyXaa: 0.0 ± 0.0
His
2.247HisAla: 2.247 ± 0.366
0.107HisCys: 0.107 ± 0.071
0.91HisAsp: 0.91 ± 0.254
1.498HisGlu: 1.498 ± 0.286
0.589HisPhe: 0.589 ± 0.19
2.14HisGly: 2.14 ± 0.357
0.642HisHis: 0.642 ± 0.204
0.91HisIle: 0.91 ± 0.242
0.696HisLys: 0.696 ± 0.2
1.98HisLeu: 1.98 ± 0.311
0.214HisMet: 0.214 ± 0.119
0.428HisAsn: 0.428 ± 0.156
1.926HisPro: 1.926 ± 0.332
0.642HisGln: 0.642 ± 0.203
1.445HisArg: 1.445 ± 0.259
1.231HisSer: 1.231 ± 0.316
1.124HisThr: 1.124 ± 0.302
1.712HisVal: 1.712 ± 0.269
0.321HisTrp: 0.321 ± 0.129
0.321HisTyr: 0.321 ± 0.134
0.0HisXaa: 0.0 ± 0.0
Ile
5.083IleAla: 5.083 ± 0.527
0.214IleCys: 0.214 ± 0.1
2.568IleAsp: 2.568 ± 0.337
2.622IleGlu: 2.622 ± 0.37
0.803IlePhe: 0.803 ± 0.227
3.852IleGly: 3.852 ± 0.551
0.963IleHis: 0.963 ± 0.24
2.408IleIle: 2.408 ± 0.434
2.301IleLys: 2.301 ± 0.422
2.568IleLeu: 2.568 ± 0.384
1.124IleMet: 1.124 ± 0.241
1.712IleAsn: 1.712 ± 0.287
2.515IlePro: 2.515 ± 0.374
2.14IleGln: 2.14 ± 0.323
3.157IleArg: 3.157 ± 0.377
2.729IleSer: 2.729 ± 0.602
5.243IleThr: 5.243 ± 0.436
3.317IleVal: 3.317 ± 0.429
0.963IleTrp: 0.963 ± 0.234
1.338IleTyr: 1.338 ± 0.267
0.0IleXaa: 0.0 ± 0.0
Lys
5.457LysAla: 5.457 ± 0.502
0.375LysCys: 0.375 ± 0.159
2.301LysAsp: 2.301 ± 0.369
2.301LysGlu: 2.301 ± 0.347
1.177LysPhe: 1.177 ± 0.244
2.568LysGly: 2.568 ± 0.4
0.803LysHis: 0.803 ± 0.266
1.926LysIle: 1.926 ± 0.372
1.766LysLys: 1.766 ± 0.308
3.745LysLeu: 3.745 ± 0.43
1.231LysMet: 1.231 ± 0.3
1.659LysAsn: 1.659 ± 0.346
1.766LysPro: 1.766 ± 0.344
1.498LysGln: 1.498 ± 0.33
2.301LysArg: 2.301 ± 0.331
2.301LysSer: 2.301 ± 0.415
2.943LysThr: 2.943 ± 0.415
3.424LysVal: 3.424 ± 0.46
0.696LysTrp: 0.696 ± 0.215
1.338LysTyr: 1.338 ± 0.295
0.0LysXaa: 0.0 ± 0.0
Leu
8.346LeuAla: 8.346 ± 0.674
0.535LeuCys: 0.535 ± 0.197
4.494LeuAsp: 4.494 ± 0.415
5.083LeuGlu: 5.083 ± 0.644
1.231LeuPhe: 1.231 ± 0.331
5.832LeuGly: 5.832 ± 0.6
2.408LeuHis: 2.408 ± 0.424
3.638LeuIle: 3.638 ± 0.376
3.478LeuLys: 3.478 ± 0.421
5.029LeuLeu: 5.029 ± 0.603
1.605LeuMet: 1.605 ± 0.304
3.103LeuAsn: 3.103 ± 0.443
4.601LeuPro: 4.601 ± 0.489
2.247LeuGln: 2.247 ± 0.328
4.601LeuArg: 4.601 ± 0.584
4.494LeuSer: 4.494 ± 0.577
5.992LeuThr: 5.992 ± 0.582
4.494LeuVal: 4.494 ± 0.442
1.177LeuTrp: 1.177 ± 0.227
1.926LeuTyr: 1.926 ± 0.327
0.0LeuXaa: 0.0 ± 0.0
Met
3.852MetAla: 3.852 ± 0.5
0.268MetCys: 0.268 ± 0.101
1.284MetAsp: 1.284 ± 0.268
1.766MetGlu: 1.766 ± 0.285
0.321MetPhe: 0.321 ± 0.136
1.498MetGly: 1.498 ± 0.325
0.428MetHis: 0.428 ± 0.16
0.482MetIle: 0.482 ± 0.175
0.91MetLys: 0.91 ± 0.207
1.391MetLeu: 1.391 ± 0.277
0.428MetMet: 0.428 ± 0.146
1.177MetAsn: 1.177 ± 0.261
1.873MetPro: 1.873 ± 0.376
0.91MetGln: 0.91 ± 0.182
1.07MetArg: 1.07 ± 0.215
1.819MetSer: 1.819 ± 0.275
2.675MetThr: 2.675 ± 0.37
1.766MetVal: 1.766 ± 0.278
0.428MetTrp: 0.428 ± 0.167
0.161MetTyr: 0.161 ± 0.099
0.0MetXaa: 0.0 ± 0.0
Asn
3.692AsnAla: 3.692 ± 0.639
0.321AsnCys: 0.321 ± 0.135
1.07AsnAsp: 1.07 ± 0.241
1.445AsnGlu: 1.445 ± 0.32
1.177AsnPhe: 1.177 ± 0.381
3.371AsnGly: 3.371 ± 0.402
0.803AsnHis: 0.803 ± 0.185
1.712AsnIle: 1.712 ± 0.289
1.017AsnLys: 1.017 ± 0.234
2.515AsnLeu: 2.515 ± 0.367
0.642AsnMet: 0.642 ± 0.185
0.803AsnAsn: 0.803 ± 0.221
2.408AsnPro: 2.408 ± 0.381
0.91AsnGln: 0.91 ± 0.246
2.461AsnArg: 2.461 ± 0.398
1.98AsnSer: 1.98 ± 0.363
2.14AsnThr: 2.14 ± 0.324
2.301AsnVal: 2.301 ± 0.349
0.803AsnTrp: 0.803 ± 0.253
1.177AsnTyr: 1.177 ± 0.251
0.0AsnXaa: 0.0 ± 0.0
Pro
7.758ProAla: 7.758 ± 0.765
0.375ProCys: 0.375 ± 0.147
3.103ProAsp: 3.103 ± 0.388
4.601ProGlu: 4.601 ± 0.64
1.284ProPhe: 1.284 ± 0.233
5.618ProGly: 5.618 ± 0.541
1.338ProHis: 1.338 ± 0.295
2.301ProIle: 2.301 ± 0.358
2.14ProLys: 2.14 ± 0.366
3.478ProLeu: 3.478 ± 0.462
1.552ProMet: 1.552 ± 0.263
2.194ProAsn: 2.194 ± 0.378
3.264ProPro: 3.264 ± 0.597
2.461ProGln: 2.461 ± 0.527
2.836ProArg: 2.836 ± 0.441
2.996ProSer: 2.996 ± 0.536
2.836ProThr: 2.836 ± 0.431
4.548ProVal: 4.548 ± 0.541
1.07ProTrp: 1.07 ± 0.224
1.445ProTyr: 1.445 ± 0.232
0.0ProXaa: 0.0 ± 0.0
Gln
3.478GlnAla: 3.478 ± 0.447
0.214GlnCys: 0.214 ± 0.101
1.445GlnAsp: 1.445 ± 0.317
1.766GlnGlu: 1.766 ± 0.396
1.552GlnPhe: 1.552 ± 0.332
3.852GlnGly: 3.852 ± 0.645
0.91GlnHis: 0.91 ± 0.213
1.498GlnIle: 1.498 ± 0.291
0.803GlnLys: 0.803 ± 0.218
2.782GlnLeu: 2.782 ± 0.425
0.749GlnMet: 0.749 ± 0.259
1.284GlnAsn: 1.284 ± 0.214
2.461GlnPro: 2.461 ± 0.458
2.515GlnGln: 2.515 ± 0.907
2.515GlnArg: 2.515 ± 0.389
1.552GlnSer: 1.552 ± 0.306
2.515GlnThr: 2.515 ± 0.351
2.408GlnVal: 2.408 ± 0.343
0.642GlnTrp: 0.642 ± 0.23
1.552GlnTyr: 1.552 ± 0.316
0.0GlnXaa: 0.0 ± 0.0
Arg
7.33ArgAla: 7.33 ± 0.768
0.696ArgCys: 0.696 ± 0.224
3.585ArgAsp: 3.585 ± 0.436
4.387ArgGlu: 4.387 ± 0.477
1.766ArgPhe: 1.766 ± 0.312
3.959ArgGly: 3.959 ± 0.606
1.338ArgHis: 1.338 ± 0.286
3.05ArgIle: 3.05 ± 0.467
2.996ArgLys: 2.996 ± 0.381
4.227ArgLeu: 4.227 ± 0.536
1.498ArgMet: 1.498 ± 0.312
1.712ArgAsn: 1.712 ± 0.277
3.478ArgPro: 3.478 ± 0.456
2.515ArgGln: 2.515 ± 0.361
4.334ArgArg: 4.334 ± 0.71
2.996ArgSer: 2.996 ± 0.439
3.959ArgThr: 3.959 ± 0.59
4.815ArgVal: 4.815 ± 0.632
1.124ArgTrp: 1.124 ± 0.269
1.766ArgTyr: 1.766 ± 0.308
0.0ArgXaa: 0.0 ± 0.0
Ser
5.832SerAla: 5.832 ± 0.88
0.589SerCys: 0.589 ± 0.183
2.515SerAsp: 2.515 ± 0.441
3.157SerGlu: 3.157 ± 0.444
1.605SerPhe: 1.605 ± 0.335
5.618SerGly: 5.618 ± 0.706
0.803SerHis: 0.803 ± 0.212
3.21SerIle: 3.21 ± 0.465
2.087SerLys: 2.087 ± 0.321
4.494SerLeu: 4.494 ± 0.532
1.712SerMet: 1.712 ± 0.303
1.659SerAsn: 1.659 ± 0.292
2.836SerPro: 2.836 ± 0.403
1.605SerGln: 1.605 ± 0.323
3.103SerArg: 3.103 ± 0.429
3.317SerSer: 3.317 ± 0.517
4.494SerThr: 4.494 ± 0.608
3.478SerVal: 3.478 ± 0.461
0.696SerTrp: 0.696 ± 0.157
1.819SerTyr: 1.819 ± 0.393
0.0SerXaa: 0.0 ± 0.0
Thr
9.684ThrAla: 9.684 ± 0.804
0.428ThrCys: 0.428 ± 0.162
3.799ThrAsp: 3.799 ± 0.371
3.424ThrGlu: 3.424 ± 0.405
1.659ThrPhe: 1.659 ± 0.338
6.688ThrGly: 6.688 ± 0.907
1.284ThrHis: 1.284 ± 0.284
4.12ThrIle: 4.12 ± 0.438
2.622ThrLys: 2.622 ± 0.352
5.564ThrLeu: 5.564 ± 0.511
1.445ThrMet: 1.445 ± 0.33
2.354ThrAsn: 2.354 ± 0.497
5.511ThrPro: 5.511 ± 0.598
2.568ThrGln: 2.568 ± 0.401
3.21ThrArg: 3.21 ± 0.457
4.655ThrSer: 4.655 ± 0.659
5.885ThrThr: 5.885 ± 0.683
5.939ThrVal: 5.939 ± 0.63
1.391ThrTrp: 1.391 ± 0.282
1.98ThrTyr: 1.98 ± 0.33
0.0ThrXaa: 0.0 ± 0.0
Val
7.651ValAla: 7.651 ± 0.634
0.642ValCys: 0.642 ± 0.178
5.029ValAsp: 5.029 ± 0.629
5.992ValGlu: 5.992 ± 0.545
2.033ValPhe: 2.033 ± 0.425
5.083ValGly: 5.083 ± 0.452
1.177ValHis: 1.177 ± 0.296
4.12ValIle: 4.12 ± 0.427
4.28ValLys: 4.28 ± 0.606
5.564ValLeu: 5.564 ± 0.513
2.087ValMet: 2.087 ± 0.329
2.729ValAsn: 2.729 ± 0.399
3.317ValPro: 3.317 ± 0.378
2.515ValGln: 2.515 ± 0.374
4.815ValArg: 4.815 ± 0.575
3.531ValSer: 3.531 ± 0.647
6.367ValThr: 6.367 ± 0.677
5.457ValVal: 5.457 ± 0.555
1.231ValTrp: 1.231 ± 0.271
1.659ValTyr: 1.659 ± 0.299
0.0ValXaa: 0.0 ± 0.0
Trp
1.445TrpAla: 1.445 ± 0.228
0.268TrpCys: 0.268 ± 0.185
1.659TrpAsp: 1.659 ± 0.266
0.696TrpGlu: 0.696 ± 0.2
0.696TrpPhe: 0.696 ± 0.15
1.98TrpGly: 1.98 ± 0.38
0.375TrpHis: 0.375 ± 0.156
0.482TrpIle: 0.482 ± 0.163
0.803TrpLys: 0.803 ± 0.197
1.124TrpLeu: 1.124 ± 0.245
0.214TrpMet: 0.214 ± 0.102
1.338TrpAsn: 1.338 ± 0.339
0.696TrpPro: 0.696 ± 0.163
0.642TrpGln: 0.642 ± 0.199
1.391TrpArg: 1.391 ± 0.252
0.963TrpSer: 0.963 ± 0.206
1.766TrpThr: 1.766 ± 0.335
1.391TrpVal: 1.391 ± 0.226
0.268TrpTrp: 0.268 ± 0.095
0.428TrpTyr: 0.428 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.766TyrAla: 1.766 ± 0.322
0.321TyrCys: 0.321 ± 0.13
2.033TyrAsp: 2.033 ± 0.429
0.963TyrGlu: 0.963 ± 0.206
0.589TyrPhe: 0.589 ± 0.212
2.515TyrGly: 2.515 ± 0.39
0.589TyrHis: 0.589 ± 0.187
1.124TyrIle: 1.124 ± 0.253
1.284TyrLys: 1.284 ± 0.231
2.889TyrLeu: 2.889 ± 0.447
0.482TyrMet: 0.482 ± 0.149
1.07TyrAsn: 1.07 ± 0.25
1.659TyrPro: 1.659 ± 0.254
0.535TyrGln: 0.535 ± 0.176
1.98TyrArg: 1.98 ± 0.337
1.605TyrSer: 1.605 ± 0.292
2.247TyrThr: 2.247 ± 0.392
2.087TyrVal: 2.087 ± 0.357
0.482TyrTrp: 0.482 ± 0.187
0.856TyrTyr: 0.856 ± 0.224
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 98 proteins (18692 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski