Amino acid dipepetide frequency for Iodobacter phage PhiPLPE

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.293AlaAla: 11.293 ± 1.47
1.344AlaCys: 1.344 ± 0.367
6.991AlaAsp: 6.991 ± 0.653
6.05AlaGlu: 6.05 ± 0.818
3.159AlaPhe: 3.159 ± 0.495
7.462AlaGly: 7.462 ± 0.751
1.143AlaHis: 1.143 ± 0.238
6.386AlaIle: 6.386 ± 0.672
5.848AlaLys: 5.848 ± 0.761
7.462AlaLeu: 7.462 ± 0.781
3.227AlaMet: 3.227 ± 0.456
4.437AlaAsn: 4.437 ± 0.538
3.294AlaPro: 3.294 ± 0.382
4.168AlaGln: 4.168 ± 0.683
3.764AlaArg: 3.764 ± 0.673
7.865AlaSer: 7.865 ± 0.718
5.311AlaThr: 5.311 ± 0.67
6.991AlaVal: 6.991 ± 0.821
1.277AlaTrp: 1.277 ± 0.309
2.891AlaTyr: 2.891 ± 0.483
0.0AlaXaa: 0.0 ± 0.0
Cys
1.076CysAla: 1.076 ± 0.307
0.067CysCys: 0.067 ± 0.078
1.143CysAsp: 1.143 ± 0.322
1.546CysGlu: 1.546 ± 0.277
0.134CysPhe: 0.134 ± 0.097
1.344CysGly: 1.344 ± 0.258
0.403CysHis: 0.403 ± 0.191
0.739CysIle: 0.739 ± 0.225
1.008CysLys: 1.008 ± 0.238
0.739CysLeu: 0.739 ± 0.22
0.202CysMet: 0.202 ± 0.153
0.605CysAsn: 0.605 ± 0.202
0.471CysPro: 0.471 ± 0.158
0.202CysGln: 0.202 ± 0.114
0.538CysArg: 0.538 ± 0.19
0.739CysSer: 0.739 ± 0.315
0.807CysThr: 0.807 ± 0.244
0.941CysVal: 0.941 ± 0.298
0.202CysTrp: 0.202 ± 0.149
0.336CysTyr: 0.336 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
6.857AspAla: 6.857 ± 0.771
0.672AspCys: 0.672 ± 0.229
3.899AspAsp: 3.899 ± 0.539
4.033AspGlu: 4.033 ± 0.603
2.689AspPhe: 2.689 ± 0.442
5.311AspGly: 5.311 ± 0.537
0.739AspHis: 0.739 ± 0.202
3.697AspIle: 3.697 ± 0.539
2.823AspLys: 2.823 ± 0.409
4.84AspLeu: 4.84 ± 0.533
1.681AspMet: 1.681 ± 0.386
2.756AspAsn: 2.756 ± 0.478
2.487AspPro: 2.487 ± 0.403
2.487AspGln: 2.487 ± 0.404
2.286AspArg: 2.286 ± 0.391
4.033AspSer: 4.033 ± 0.631
3.563AspThr: 3.563 ± 0.398
4.504AspVal: 4.504 ± 0.45
0.739AspTrp: 0.739 ± 0.242
2.218AspTyr: 2.218 ± 0.438
0.0AspXaa: 0.0 ± 0.0
Glu
5.243GluAla: 5.243 ± 0.999
1.21GluCys: 1.21 ± 0.309
3.092GluAsp: 3.092 ± 0.425
4.571GluGlu: 4.571 ± 0.953
3.227GluPhe: 3.227 ± 0.396
3.227GluGly: 3.227 ± 0.502
1.21GluHis: 1.21 ± 0.293
4.302GluIle: 4.302 ± 0.516
4.706GluLys: 4.706 ± 0.696
5.243GluLeu: 5.243 ± 0.664
1.815GluMet: 1.815 ± 0.293
2.42GluAsn: 2.42 ± 0.383
0.941GluPro: 0.941 ± 0.223
2.151GluGln: 2.151 ± 0.469
3.428GluArg: 3.428 ± 0.626
3.764GluSer: 3.764 ± 0.461
2.487GluThr: 2.487 ± 0.459
3.227GluVal: 3.227 ± 0.479
1.479GluTrp: 1.479 ± 0.313
2.151GluTyr: 2.151 ± 0.335
0.0GluXaa: 0.0 ± 0.0
Phe
2.958PheAla: 2.958 ± 0.549
0.605PheCys: 0.605 ± 0.183
2.286PheAsp: 2.286 ± 0.468
1.412PheGlu: 1.412 ± 0.278
1.681PhePhe: 1.681 ± 0.422
2.622PheGly: 2.622 ± 0.374
0.269PheHis: 0.269 ± 0.15
1.949PheIle: 1.949 ± 0.341
2.756PheLys: 2.756 ± 0.367
1.949PheLeu: 1.949 ± 0.291
1.008PheMet: 1.008 ± 0.252
2.151PheAsn: 2.151 ± 0.441
1.008PhePro: 1.008 ± 0.224
0.739PheGln: 0.739 ± 0.241
1.613PheArg: 1.613 ± 0.414
2.42PheSer: 2.42 ± 0.456
2.756PheThr: 2.756 ± 0.418
3.092PheVal: 3.092 ± 0.435
0.336PheTrp: 0.336 ± 0.123
1.21PheTyr: 1.21 ± 0.269
0.0PheXaa: 0.0 ± 0.0
Gly
6.453GlyAla: 6.453 ± 0.665
0.538GlyCys: 0.538 ± 0.176
4.974GlyAsp: 4.974 ± 0.734
3.63GlyGlu: 3.63 ± 0.419
2.823GlyPhe: 2.823 ± 0.382
6.319GlyGly: 6.319 ± 0.813
0.672GlyHis: 0.672 ± 0.175
4.571GlyIle: 4.571 ± 0.666
4.369GlyLys: 4.369 ± 0.603
5.848GlyLeu: 5.848 ± 0.555
2.353GlyMet: 2.353 ± 0.378
3.227GlyAsn: 3.227 ± 0.467
1.681GlyPro: 1.681 ± 0.311
2.891GlyGln: 2.891 ± 0.448
3.563GlyArg: 3.563 ± 0.491
5.378GlySer: 5.378 ± 0.732
4.101GlyThr: 4.101 ± 0.662
6.655GlyVal: 6.655 ± 0.621
1.21GlyTrp: 1.21 ± 0.269
3.092GlyTyr: 3.092 ± 0.517
0.0GlyXaa: 0.0 ± 0.0
His
0.739HisAla: 0.739 ± 0.25
0.269HisCys: 0.269 ± 0.132
0.807HisAsp: 0.807 ± 0.288
0.739HisGlu: 0.739 ± 0.22
0.403HisPhe: 0.403 ± 0.158
0.739HisGly: 0.739 ± 0.213
0.134HisHis: 0.134 ± 0.087
0.672HisIle: 0.672 ± 0.203
0.807HisLys: 0.807 ± 0.224
1.008HisLeu: 1.008 ± 0.256
0.134HisMet: 0.134 ± 0.089
1.277HisAsn: 1.277 ± 0.268
0.807HisPro: 0.807 ± 0.211
0.605HisGln: 0.605 ± 0.186
0.403HisArg: 0.403 ± 0.167
0.739HisSer: 0.739 ± 0.193
0.605HisThr: 0.605 ± 0.241
0.807HisVal: 0.807 ± 0.248
0.134HisTrp: 0.134 ± 0.092
0.807HisTyr: 0.807 ± 0.305
0.0HisXaa: 0.0 ± 0.0
Ile
6.655IleAla: 6.655 ± 0.624
0.874IleCys: 0.874 ± 0.331
5.311IleAsp: 5.311 ± 0.57
4.504IleGlu: 4.504 ± 0.543
1.613IlePhe: 1.613 ± 0.367
4.84IleGly: 4.84 ± 0.625
1.008IleHis: 1.008 ± 0.234
4.504IleIle: 4.504 ± 0.595
3.966IleLys: 3.966 ± 0.551
3.764IleLeu: 3.764 ± 0.516
1.277IleMet: 1.277 ± 0.346
3.966IleAsn: 3.966 ± 0.435
2.42IlePro: 2.42 ± 0.359
1.949IleGln: 1.949 ± 0.335
2.42IleArg: 2.42 ± 0.404
4.168IleSer: 4.168 ± 0.49
4.437IleThr: 4.437 ± 0.534
3.832IleVal: 3.832 ± 0.453
0.538IleTrp: 0.538 ± 0.195
2.084IleTyr: 2.084 ± 0.442
0.0IleXaa: 0.0 ± 0.0
Lys
7.462LysAla: 7.462 ± 0.789
0.874LysCys: 0.874 ± 0.199
3.63LysAsp: 3.63 ± 0.599
4.369LysGlu: 4.369 ± 0.798
1.949LysPhe: 1.949 ± 0.402
4.235LysGly: 4.235 ± 0.551
0.538LysHis: 0.538 ± 0.211
3.764LysIle: 3.764 ± 0.424
4.235LysLys: 4.235 ± 0.588
5.042LysLeu: 5.042 ± 0.57
2.017LysMet: 2.017 ± 0.39
2.689LysAsn: 2.689 ± 0.467
3.294LysPro: 3.294 ± 0.487
2.353LysGln: 2.353 ± 0.363
3.428LysArg: 3.428 ± 0.628
3.899LysSer: 3.899 ± 0.455
3.227LysThr: 3.227 ± 0.392
3.764LysVal: 3.764 ± 0.441
1.143LysTrp: 1.143 ± 0.251
2.017LysTyr: 2.017 ± 0.389
0.0LysXaa: 0.0 ± 0.0
Leu
7.126LeuAla: 7.126 ± 0.866
1.143LeuCys: 1.143 ± 0.3
5.983LeuAsp: 5.983 ± 0.738
4.302LeuGlu: 4.302 ± 0.492
1.748LeuPhe: 1.748 ± 0.399
5.647LeuGly: 5.647 ± 0.703
1.008LeuHis: 1.008 ± 0.262
4.773LeuIle: 4.773 ± 0.451
4.907LeuLys: 4.907 ± 0.589
4.974LeuLeu: 4.974 ± 0.65
2.218LeuMet: 2.218 ± 0.358
3.899LeuAsn: 3.899 ± 0.52
2.958LeuPro: 2.958 ± 0.36
2.823LeuGln: 2.823 ± 0.502
3.966LeuArg: 3.966 ± 0.546
5.512LeuSer: 5.512 ± 0.58
4.571LeuThr: 4.571 ± 0.566
3.966LeuVal: 3.966 ± 0.557
1.143LeuTrp: 1.143 ± 0.313
2.218LeuTyr: 2.218 ± 0.42
0.0LeuXaa: 0.0 ± 0.0
Met
3.025MetAla: 3.025 ± 0.345
0.269MetCys: 0.269 ± 0.127
0.739MetAsp: 0.739 ± 0.184
0.874MetGlu: 0.874 ± 0.192
1.143MetPhe: 1.143 ± 0.28
1.412MetGly: 1.412 ± 0.296
0.134MetHis: 0.134 ± 0.113
1.613MetIle: 1.613 ± 0.332
2.353MetLys: 2.353 ± 0.385
2.554MetLeu: 2.554 ± 0.366
0.739MetMet: 0.739 ± 0.22
1.748MetAsn: 1.748 ± 0.328
1.681MetPro: 1.681 ± 0.278
1.008MetGln: 1.008 ± 0.292
1.681MetArg: 1.681 ± 0.41
2.286MetSer: 2.286 ± 0.328
2.622MetThr: 2.622 ± 0.349
1.277MetVal: 1.277 ± 0.256
0.269MetTrp: 0.269 ± 0.115
0.538MetTyr: 0.538 ± 0.208
0.0MetXaa: 0.0 ± 0.0
Asn
3.764AsnAla: 3.764 ± 0.397
0.403AsnCys: 0.403 ± 0.17
2.958AsnAsp: 2.958 ± 0.352
2.756AsnGlu: 2.756 ± 0.378
1.412AsnPhe: 1.412 ± 0.281
4.974AsnGly: 4.974 ± 0.704
1.008AsnHis: 1.008 ± 0.247
3.361AsnIle: 3.361 ± 0.521
3.294AsnLys: 3.294 ± 0.506
3.294AsnLeu: 3.294 ± 0.544
0.874AsnMet: 0.874 ± 0.247
2.622AsnAsn: 2.622 ± 0.387
2.689AsnPro: 2.689 ± 0.444
1.882AsnGln: 1.882 ± 0.367
2.286AsnArg: 2.286 ± 0.367
2.689AsnSer: 2.689 ± 0.6
3.092AsnThr: 3.092 ± 0.448
3.428AsnVal: 3.428 ± 0.532
1.008AsnTrp: 1.008 ± 0.255
1.815AsnTyr: 1.815 ± 0.383
0.0AsnXaa: 0.0 ± 0.0
Pro
5.378ProAla: 5.378 ± 0.671
0.471ProCys: 0.471 ± 0.18
2.823ProAsp: 2.823 ± 0.373
1.949ProGlu: 1.949 ± 0.275
1.344ProPhe: 1.344 ± 0.335
1.949ProGly: 1.949 ± 0.366
0.202ProHis: 0.202 ± 0.107
2.353ProIle: 2.353 ± 0.394
2.084ProLys: 2.084 ± 0.39
2.756ProLeu: 2.756 ± 0.401
0.739ProMet: 0.739 ± 0.213
2.084ProAsn: 2.084 ± 0.496
1.546ProPro: 1.546 ± 0.327
1.21ProGln: 1.21 ± 0.274
1.479ProArg: 1.479 ± 0.307
3.294ProSer: 3.294 ± 0.499
2.218ProThr: 2.218 ± 0.452
2.554ProVal: 2.554 ± 0.414
0.403ProTrp: 0.403 ± 0.169
1.277ProTyr: 1.277 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
4.033GlnAla: 4.033 ± 0.767
0.538GlnCys: 0.538 ± 0.191
1.748GlnAsp: 1.748 ± 0.342
1.815GlnGlu: 1.815 ± 0.337
1.412GlnPhe: 1.412 ± 0.425
3.159GlnGly: 3.159 ± 0.476
0.471GlnHis: 0.471 ± 0.187
2.891GlnIle: 2.891 ± 0.349
1.681GlnLys: 1.681 ± 0.325
3.428GlnLeu: 3.428 ± 0.429
1.277GlnMet: 1.277 ± 0.277
1.412GlnAsn: 1.412 ± 0.286
1.143GlnPro: 1.143 ± 0.322
1.277GlnGln: 1.277 ± 0.278
1.815GlnArg: 1.815 ± 0.368
1.681GlnSer: 1.681 ± 0.387
2.353GlnThr: 2.353 ± 0.385
1.748GlnVal: 1.748 ± 0.277
0.605GlnTrp: 0.605 ± 0.203
1.21GlnTyr: 1.21 ± 0.313
0.0GlnXaa: 0.0 ± 0.0
Arg
4.974ArgAla: 4.974 ± 0.577
0.739ArgCys: 0.739 ± 0.208
2.218ArgAsp: 2.218 ± 0.413
3.294ArgGlu: 3.294 ± 0.756
1.681ArgPhe: 1.681 ± 0.369
1.815ArgGly: 1.815 ± 0.323
0.672ArgHis: 0.672 ± 0.195
3.697ArgIle: 3.697 ± 0.481
3.025ArgLys: 3.025 ± 0.507
3.361ArgLeu: 3.361 ± 0.481
1.546ArgMet: 1.546 ± 0.319
1.949ArgAsn: 1.949 ± 0.379
2.151ArgPro: 2.151 ± 0.446
1.277ArgGln: 1.277 ± 0.299
2.218ArgArg: 2.218 ± 0.387
2.622ArgSer: 2.622 ± 0.435
2.151ArgThr: 2.151 ± 0.407
3.697ArgVal: 3.697 ± 0.639
1.21ArgTrp: 1.21 ± 0.275
1.613ArgTyr: 1.613 ± 0.377
0.0ArgXaa: 0.0 ± 0.0
Ser
6.655SerAla: 6.655 ± 0.533
0.672SerCys: 0.672 ± 0.235
3.63SerAsp: 3.63 ± 0.497
4.302SerGlu: 4.302 ± 0.68
2.218SerPhe: 2.218 ± 0.382
6.521SerGly: 6.521 ± 0.631
1.008SerHis: 1.008 ± 0.263
4.235SerIle: 4.235 ± 0.553
4.504SerLys: 4.504 ± 0.518
4.369SerLeu: 4.369 ± 0.576
1.815SerMet: 1.815 ± 0.301
3.025SerAsn: 3.025 ± 0.463
2.42SerPro: 2.42 ± 0.442
2.622SerGln: 2.622 ± 0.461
2.353SerArg: 2.353 ± 0.392
3.697SerSer: 3.697 ± 0.748
4.369SerThr: 4.369 ± 0.632
5.176SerVal: 5.176 ± 0.597
1.008SerTrp: 1.008 ± 0.303
2.689SerTyr: 2.689 ± 0.386
0.0SerXaa: 0.0 ± 0.0
Thr
5.848ThrAla: 5.848 ± 0.745
0.807ThrCys: 0.807 ± 0.226
3.63ThrAsp: 3.63 ± 0.623
3.563ThrGlu: 3.563 ± 0.505
1.546ThrPhe: 1.546 ± 0.279
5.916ThrGly: 5.916 ± 0.883
0.739ThrHis: 0.739 ± 0.185
3.832ThrIle: 3.832 ± 0.509
3.899ThrLys: 3.899 ± 0.606
4.638ThrLeu: 4.638 ± 0.674
1.143ThrMet: 1.143 ± 0.312
2.554ThrAsn: 2.554 ± 0.456
2.823ThrPro: 2.823 ± 0.41
1.546ThrGln: 1.546 ± 0.406
2.958ThrArg: 2.958 ± 0.482
4.235ThrSer: 4.235 ± 0.659
2.756ThrThr: 2.756 ± 0.38
4.101ThrVal: 4.101 ± 0.726
0.605ThrTrp: 0.605 ± 0.229
1.882ThrTyr: 1.882 ± 0.397
0.0ThrXaa: 0.0 ± 0.0
Val
5.714ValAla: 5.714 ± 0.73
0.739ValCys: 0.739 ± 0.232
3.832ValAsp: 3.832 ± 0.474
3.697ValGlu: 3.697 ± 0.401
2.353ValPhe: 2.353 ± 0.38
3.294ValGly: 3.294 ± 0.526
0.739ValHis: 0.739 ± 0.241
4.638ValIle: 4.638 ± 0.533
4.907ValLys: 4.907 ± 0.569
4.907ValLeu: 4.907 ± 0.51
2.286ValMet: 2.286 ± 0.365
4.101ValAsn: 4.101 ± 0.548
2.756ValPro: 2.756 ± 0.435
2.353ValGln: 2.353 ± 0.339
2.958ValArg: 2.958 ± 0.43
5.378ValSer: 5.378 ± 0.597
4.907ValThr: 4.907 ± 0.553
4.235ValVal: 4.235 ± 0.689
0.807ValTrp: 0.807 ± 0.209
2.353ValTyr: 2.353 ± 0.43
0.0ValXaa: 0.0 ± 0.0
Trp
1.412TrpAla: 1.412 ± 0.243
0.336TrpCys: 0.336 ± 0.146
0.807TrpAsp: 0.807 ± 0.245
0.538TrpGlu: 0.538 ± 0.197
0.605TrpPhe: 0.605 ± 0.198
0.874TrpGly: 0.874 ± 0.271
0.403TrpHis: 0.403 ± 0.146
0.739TrpIle: 0.739 ± 0.237
0.605TrpLys: 0.605 ± 0.174
1.748TrpLeu: 1.748 ± 0.342
0.403TrpMet: 0.403 ± 0.186
0.807TrpAsn: 0.807 ± 0.236
0.471TrpPro: 0.471 ± 0.194
0.941TrpGln: 0.941 ± 0.234
0.739TrpArg: 0.739 ± 0.213
1.008TrpSer: 1.008 ± 0.244
0.739TrpThr: 0.739 ± 0.234
1.076TrpVal: 1.076 ± 0.268
0.336TrpTrp: 0.336 ± 0.145
0.336TrpTyr: 0.336 ± 0.139
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.697TyrAla: 3.697 ± 0.474
0.672TyrCys: 0.672 ± 0.323
1.815TyrAsp: 1.815 ± 0.324
1.949TyrGlu: 1.949 ± 0.269
1.681TyrPhe: 1.681 ± 0.25
2.689TyrGly: 2.689 ± 0.435
0.134TyrHis: 0.134 ± 0.102
1.412TyrIle: 1.412 ± 0.268
2.151TyrLys: 2.151 ± 0.349
2.958TyrLeu: 2.958 ± 0.482
1.143TyrMet: 1.143 ± 0.301
1.882TyrAsn: 1.882 ± 0.323
1.076TyrPro: 1.076 ± 0.251
1.21TyrGln: 1.21 ± 0.293
2.017TyrArg: 2.017 ± 0.374
1.949TyrSer: 1.949 ± 0.34
1.949TyrThr: 1.949 ± 0.35
1.882TyrVal: 1.882 ± 0.396
0.471TyrTrp: 0.471 ± 0.201
1.21TyrTyr: 1.21 ± 0.296
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (14877 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski