Amino acid dipepetide frequency for Clostridium phage phi24R

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.504AlaCys: 0.504 ± 0.419
1.345AlaAsp: 1.345 ± 0.543
2.354AlaGlu: 2.354 ± 0.599
1.513AlaPhe: 1.513 ± 0.446
0.504AlaGly: 0.504 ± 0.245
0.168AlaHis: 0.168 ± 0.16
2.69AlaIle: 2.69 ± 0.423
2.354AlaLys: 2.354 ± 0.56
3.195AlaLeu: 3.195 ± 0.635
0.673AlaMet: 0.673 ± 0.297
3.867AlaAsn: 3.867 ± 1.272
0.336AlaPro: 0.336 ± 0.249
1.177AlaGln: 1.177 ± 0.503
1.85AlaArg: 1.85 ± 0.766
2.859AlaSer: 2.859 ± 0.786
2.018AlaThr: 2.018 ± 0.535
2.354AlaVal: 2.354 ± 0.662
0.168AlaTrp: 0.168 ± 0.16
2.018AlaTyr: 2.018 ± 0.696
0.0AlaXaa: 0.0 ± 0.0
Cys
0.168CysAla: 0.168 ± 0.151
0.168CysCys: 0.168 ± 0.16
1.009CysAsp: 1.009 ± 0.456
0.504CysGlu: 0.504 ± 0.249
0.673CysPhe: 0.673 ± 0.385
1.009CysGly: 1.009 ± 0.408
0.336CysHis: 0.336 ± 0.234
0.841CysIle: 0.841 ± 0.441
1.85CysLys: 1.85 ± 0.665
1.177CysLeu: 1.177 ± 0.528
0.336CysMet: 0.336 ± 0.253
1.345CysAsn: 1.345 ± 0.47
0.168CysPro: 0.168 ± 0.192
0.841CysGln: 0.841 ± 0.38
0.168CysArg: 0.168 ± 0.16
1.682CysSer: 1.682 ± 0.49
0.336CysThr: 0.336 ± 0.195
1.009CysVal: 1.009 ± 0.457
0.168CysTrp: 0.168 ± 0.16
0.673CysTyr: 0.673 ± 0.243
0.0CysXaa: 0.0 ± 0.0
Asp
2.354AspAla: 2.354 ± 0.486
0.168AspCys: 0.168 ± 0.161
1.85AspAsp: 1.85 ± 0.513
4.204AspGlu: 4.204 ± 0.952
2.859AspPhe: 2.859 ± 0.749
6.053AspGly: 6.053 ± 0.984
0.336AspHis: 0.336 ± 0.219
5.213AspIle: 5.213 ± 0.909
7.231AspLys: 7.231 ± 1.017
6.053AspLeu: 6.053 ± 1.13
1.345AspMet: 1.345 ± 0.398
5.549AspAsn: 5.549 ± 1.09
1.177AspPro: 1.177 ± 0.389
0.0AspGln: 0.0 ± 0.0
2.69AspArg: 2.69 ± 0.46
3.027AspSer: 3.027 ± 0.64
4.204AspThr: 4.204 ± 0.701
2.859AspVal: 2.859 ± 0.854
0.168AspTrp: 0.168 ± 0.14
3.531AspTyr: 3.531 ± 0.665
0.0AspXaa: 0.0 ± 0.0
Glu
1.009GluAla: 1.009 ± 0.489
0.673GluCys: 0.673 ± 0.32
3.027GluAsp: 3.027 ± 0.814
5.381GluGlu: 5.381 ± 1.196
3.027GluPhe: 3.027 ± 0.887
4.204GluGly: 4.204 ± 0.87
1.177GluHis: 1.177 ± 0.441
8.239GluIle: 8.239 ± 1.187
8.744GluLys: 8.744 ± 1.423
6.894GluLeu: 6.894 ± 1.107
2.018GluMet: 2.018 ± 0.448
8.576GluAsn: 8.576 ± 1.498
1.345GluPro: 1.345 ± 0.523
1.682GluGln: 1.682 ± 0.792
2.186GluArg: 2.186 ± 0.644
4.54GluSer: 4.54 ± 0.584
5.045GluThr: 5.045 ± 0.731
6.222GluVal: 6.222 ± 1.367
0.673GluTrp: 0.673 ± 0.361
5.045GluTyr: 5.045 ± 1.015
0.0GluXaa: 0.0 ± 0.0
Phe
1.177PheAla: 1.177 ± 0.335
0.673PheCys: 0.673 ± 0.271
3.027PheAsp: 3.027 ± 0.717
4.372PheGlu: 4.372 ± 0.869
1.85PhePhe: 1.85 ± 0.718
3.363PheGly: 3.363 ± 0.732
0.673PheHis: 0.673 ± 0.308
3.867PheIle: 3.867 ± 0.685
4.372PheLys: 4.372 ± 0.904
4.204PheLeu: 4.204 ± 0.656
1.009PheMet: 1.009 ± 0.411
4.54PheAsn: 4.54 ± 1.016
1.009PhePro: 1.009 ± 0.438
0.841PheGln: 0.841 ± 0.385
1.009PheArg: 1.009 ± 0.374
2.186PheSer: 2.186 ± 0.624
2.354PheThr: 2.354 ± 0.695
2.859PheVal: 2.859 ± 0.639
0.168PheTrp: 0.168 ± 0.151
3.027PheTyr: 3.027 ± 0.94
0.0PheXaa: 0.0 ± 0.0
Gly
3.195GlyAla: 3.195 ± 0.794
0.841GlyCys: 0.841 ± 0.452
5.381GlyAsp: 5.381 ± 1.068
5.045GlyGlu: 5.045 ± 0.922
3.363GlyPhe: 3.363 ± 0.688
3.531GlyGly: 3.531 ± 1.008
0.504GlyHis: 0.504 ± 0.359
4.036GlyIle: 4.036 ± 1.238
4.876GlyLys: 4.876 ± 0.736
7.903GlyLeu: 7.903 ± 0.981
1.345GlyMet: 1.345 ± 0.451
4.204GlyAsn: 4.204 ± 1.178
0.0GlyPro: 0.0 ± 0.0
2.018GlyGln: 2.018 ± 0.876
1.513GlyArg: 1.513 ± 0.543
2.69GlySer: 2.69 ± 0.699
3.027GlyThr: 3.027 ± 1.098
4.876GlyVal: 4.876 ± 0.691
0.673GlyTrp: 0.673 ± 0.344
2.522GlyTyr: 2.522 ± 0.513
0.0GlyXaa: 0.0 ± 0.0
His
0.168HisAla: 0.168 ± 0.201
0.168HisCys: 0.168 ± 0.163
0.841HisAsp: 0.841 ± 0.306
1.009HisGlu: 1.009 ± 0.384
0.841HisPhe: 0.841 ± 0.315
1.682HisGly: 1.682 ± 0.479
0.0HisHis: 0.0 ± 0.0
0.841HisIle: 0.841 ± 0.27
0.504HisLys: 0.504 ± 0.237
1.009HisLeu: 1.009 ± 0.339
0.168HisMet: 0.168 ± 0.17
0.673HisAsn: 0.673 ± 0.326
0.336HisPro: 0.336 ± 0.245
0.0HisGln: 0.0 ± 0.0
0.168HisArg: 0.168 ± 0.151
0.673HisSer: 0.673 ± 0.269
1.009HisThr: 1.009 ± 0.431
0.504HisVal: 0.504 ± 0.245
0.168HisTrp: 0.168 ± 0.16
0.673HisTyr: 0.673 ± 0.427
0.0HisXaa: 0.0 ± 0.0
Ile
3.027IleAla: 3.027 ± 1.223
1.177IleCys: 1.177 ± 0.384
6.726IleAsp: 6.726 ± 1.011
7.399IleGlu: 7.399 ± 1.361
3.699IlePhe: 3.699 ± 0.892
5.381IleGly: 5.381 ± 1.034
0.841IleHis: 0.841 ± 0.308
7.399IleIle: 7.399 ± 1.713
8.744IleLys: 8.744 ± 1.36
5.213IleLeu: 5.213 ± 0.742
1.513IleMet: 1.513 ± 0.502
8.744IleAsn: 8.744 ± 1.484
2.522IlePro: 2.522 ± 0.878
2.186IleGln: 2.186 ± 0.648
3.027IleArg: 3.027 ± 0.559
5.885IleSer: 5.885 ± 0.899
3.195IleThr: 3.195 ± 0.8
3.699IleVal: 3.699 ± 0.616
0.504IleTrp: 0.504 ± 0.308
3.195IleTyr: 3.195 ± 0.507
0.0IleXaa: 0.0 ± 0.0
Lys
3.363LysAla: 3.363 ± 0.551
1.85LysCys: 1.85 ± 0.692
7.231LysAsp: 7.231 ± 1.365
9.08LysGlu: 9.08 ± 2.159
3.699LysPhe: 3.699 ± 0.794
5.045LysGly: 5.045 ± 1.047
1.177LysHis: 1.177 ± 0.385
9.08LysIle: 9.08 ± 1.107
7.062LysLys: 7.062 ± 1.519
7.567LysLeu: 7.567 ± 1.088
3.195LysMet: 3.195 ± 0.923
8.071LysAsn: 8.071 ± 1.367
2.186LysPro: 2.186 ± 0.51
3.195LysGln: 3.195 ± 0.945
3.699LysArg: 3.699 ± 0.907
3.867LysSer: 3.867 ± 0.928
4.372LysThr: 4.372 ± 0.625
6.726LysVal: 6.726 ± 0.918
0.673LysTrp: 0.673 ± 0.296
3.867LysTyr: 3.867 ± 0.956
0.0LysXaa: 0.0 ± 0.0
Leu
2.69LeuAla: 2.69 ± 0.711
2.354LeuCys: 2.354 ± 0.599
5.045LeuAsp: 5.045 ± 1.164
7.735LeuGlu: 7.735 ± 0.871
3.027LeuPhe: 3.027 ± 0.619
3.699LeuGly: 3.699 ± 1.055
1.009LeuHis: 1.009 ± 0.352
6.558LeuIle: 6.558 ± 1.714
8.576LeuLys: 8.576 ± 0.942
7.231LeuLeu: 7.231 ± 1.201
2.018LeuMet: 2.018 ± 0.447
7.735LeuAsn: 7.735 ± 1.127
2.859LeuPro: 2.859 ± 0.844
1.85LeuGln: 1.85 ± 0.587
3.363LeuArg: 3.363 ± 0.811
5.549LeuSer: 5.549 ± 0.975
3.867LeuThr: 3.867 ± 0.926
3.531LeuVal: 3.531 ± 0.57
0.168LeuTrp: 0.168 ± 0.163
3.363LeuTyr: 3.363 ± 0.592
0.0LeuXaa: 0.0 ± 0.0
Met
1.513MetAla: 1.513 ± 0.6
0.336MetCys: 0.336 ± 0.234
1.85MetAsp: 1.85 ± 0.415
1.345MetGlu: 1.345 ± 0.391
1.682MetPhe: 1.682 ± 0.416
1.513MetGly: 1.513 ± 0.366
0.0MetHis: 0.0 ± 0.0
1.682MetIle: 1.682 ± 0.497
2.186MetLys: 2.186 ± 0.576
2.186MetLeu: 2.186 ± 0.674
0.168MetMet: 0.168 ± 0.161
1.345MetAsn: 1.345 ± 0.342
1.177MetPro: 1.177 ± 0.551
0.841MetGln: 0.841 ± 0.262
0.336MetArg: 0.336 ± 0.225
1.85MetSer: 1.85 ± 0.536
0.841MetThr: 0.841 ± 0.41
0.841MetVal: 0.841 ± 0.289
0.168MetTrp: 0.168 ± 0.16
1.009MetTyr: 1.009 ± 0.347
0.0MetXaa: 0.0 ± 0.0
Asn
2.354AsnAla: 2.354 ± 0.691
1.009AsnCys: 1.009 ± 0.578
4.876AsnAsp: 4.876 ± 0.838
7.567AsnGlu: 7.567 ± 0.97
2.859AsnPhe: 2.859 ± 0.738
7.062AsnGly: 7.062 ± 1.315
1.345AsnHis: 1.345 ± 0.628
9.921AsnIle: 9.921 ± 1.663
9.585AsnLys: 9.585 ± 1.459
7.567AsnLeu: 7.567 ± 1.33
2.186AsnMet: 2.186 ± 0.502
9.248AsnAsn: 9.248 ± 2.381
3.027AsnPro: 3.027 ± 0.667
3.195AsnGln: 3.195 ± 0.723
2.522AsnArg: 2.522 ± 0.586
6.222AsnSer: 6.222 ± 1.171
5.381AsnThr: 5.381 ± 1.024
3.867AsnVal: 3.867 ± 0.814
0.841AsnTrp: 0.841 ± 0.376
4.036AsnTyr: 4.036 ± 0.987
0.0AsnXaa: 0.0 ± 0.0
Pro
0.0ProAla: 0.0 ± 0.0
0.504ProCys: 0.504 ± 0.254
0.336ProAsp: 0.336 ± 0.228
0.673ProGlu: 0.673 ± 0.316
2.186ProPhe: 2.186 ± 0.431
0.168ProGly: 0.168 ± 0.163
0.336ProHis: 0.336 ± 0.238
1.513ProIle: 1.513 ± 0.512
2.186ProLys: 2.186 ± 0.574
0.841ProLeu: 0.841 ± 0.267
0.504ProMet: 0.504 ± 0.245
2.859ProAsn: 2.859 ± 0.526
0.0ProPro: 0.0 ± 0.0
0.673ProGln: 0.673 ± 0.253
0.336ProArg: 0.336 ± 0.294
2.354ProSer: 2.354 ± 0.705
2.186ProThr: 2.186 ± 0.69
2.018ProVal: 2.018 ± 0.548
0.0ProTrp: 0.0 ± 0.0
2.69ProTyr: 2.69 ± 0.567
0.0ProXaa: 0.0 ± 0.0
Gln
1.345GlnAla: 1.345 ± 0.554
0.504GlnCys: 0.504 ± 0.285
0.673GlnAsp: 0.673 ± 0.407
1.85GlnGlu: 1.85 ± 0.577
2.018GlnPhe: 2.018 ± 0.577
2.522GlnGly: 2.522 ± 0.93
0.504GlnHis: 0.504 ± 0.248
2.018GlnIle: 2.018 ± 0.603
2.186GlnLys: 2.186 ± 0.726
2.018GlnLeu: 2.018 ± 0.573
1.177GlnMet: 1.177 ± 0.444
3.195GlnAsn: 3.195 ± 0.859
0.168GlnPro: 0.168 ± 0.14
1.177GlnGln: 1.177 ± 0.504
0.673GlnArg: 0.673 ± 0.377
1.682GlnSer: 1.682 ± 0.471
1.85GlnThr: 1.85 ± 0.638
1.009GlnVal: 1.009 ± 0.424
0.168GlnTrp: 0.168 ± 0.16
1.345GlnTyr: 1.345 ± 0.591
0.0GlnXaa: 0.0 ± 0.0
Arg
1.009ArgAla: 1.009 ± 0.28
0.841ArgCys: 0.841 ± 0.328
1.177ArgAsp: 1.177 ± 0.392
2.522ArgGlu: 2.522 ± 0.598
1.682ArgPhe: 1.682 ± 0.511
2.018ArgGly: 2.018 ± 0.524
0.336ArgHis: 0.336 ± 0.232
3.027ArgIle: 3.027 ± 0.685
3.195ArgLys: 3.195 ± 1.18
3.699ArgLeu: 3.699 ± 1.213
0.336ArgMet: 0.336 ± 0.224
3.195ArgAsn: 3.195 ± 0.605
0.673ArgPro: 0.673 ± 0.35
0.504ArgGln: 0.504 ± 0.33
1.345ArgArg: 1.345 ± 0.455
1.009ArgSer: 1.009 ± 0.425
1.345ArgThr: 1.345 ± 0.648
2.018ArgVal: 2.018 ± 0.55
0.336ArgTrp: 0.336 ± 0.229
1.345ArgTyr: 1.345 ± 0.593
0.0ArgXaa: 0.0 ± 0.0
Ser
3.027SerAla: 3.027 ± 0.81
1.177SerCys: 1.177 ± 0.266
4.708SerAsp: 4.708 ± 0.483
4.54SerGlu: 4.54 ± 1.042
2.69SerPhe: 2.69 ± 0.906
2.522SerGly: 2.522 ± 0.994
0.673SerHis: 0.673 ± 0.409
5.213SerIle: 5.213 ± 1.053
5.717SerLys: 5.717 ± 1.015
5.381SerLeu: 5.381 ± 1.176
1.009SerMet: 1.009 ± 0.283
5.045SerAsn: 5.045 ± 1.087
0.673SerPro: 0.673 ± 0.386
2.186SerGln: 2.186 ± 0.793
2.018SerArg: 2.018 ± 0.441
4.204SerSer: 4.204 ± 0.957
3.699SerThr: 3.699 ± 0.873
3.363SerVal: 3.363 ± 1.037
0.168SerTrp: 0.168 ± 0.16
2.69SerTyr: 2.69 ± 0.603
0.0SerXaa: 0.0 ± 0.0
Thr
1.177ThrAla: 1.177 ± 0.539
0.504ThrCys: 0.504 ± 0.276
3.531ThrAsp: 3.531 ± 0.676
3.195ThrGlu: 3.195 ± 0.784
2.018ThrPhe: 2.018 ± 0.658
3.195ThrGly: 3.195 ± 0.901
0.673ThrHis: 0.673 ± 0.533
3.699ThrIle: 3.699 ± 0.618
3.699ThrLys: 3.699 ± 0.855
4.036ThrLeu: 4.036 ± 0.729
0.841ThrMet: 0.841 ± 0.403
5.885ThrAsn: 5.885 ± 0.883
2.018ThrPro: 2.018 ± 0.748
1.682ThrGln: 1.682 ± 0.702
2.69ThrArg: 2.69 ± 0.792
4.708ThrSer: 4.708 ± 0.594
2.018ThrThr: 2.018 ± 0.384
4.204ThrVal: 4.204 ± 0.902
0.336ThrTrp: 0.336 ± 0.195
3.027ThrTyr: 3.027 ± 0.709
0.0ThrXaa: 0.0 ± 0.0
Val
2.69ValAla: 2.69 ± 0.739
0.168ValCys: 0.168 ± 0.163
4.708ValAsp: 4.708 ± 1.084
4.54ValGlu: 4.54 ± 0.851
2.859ValPhe: 2.859 ± 0.866
4.036ValGly: 4.036 ± 1.389
1.177ValHis: 1.177 ± 0.426
5.213ValIle: 5.213 ± 0.718
6.558ValLys: 6.558 ± 0.77
1.345ValLeu: 1.345 ± 0.44
1.345ValMet: 1.345 ± 0.506
5.045ValAsn: 5.045 ± 0.842
1.177ValPro: 1.177 ± 0.376
2.018ValGln: 2.018 ± 0.64
0.841ValArg: 0.841 ± 0.501
2.522ValSer: 2.522 ± 0.663
3.027ValThr: 3.027 ± 0.621
3.867ValVal: 3.867 ± 1.148
1.513ValTrp: 1.513 ± 0.508
3.867ValTyr: 3.867 ± 0.99
0.0ValXaa: 0.0 ± 0.0
Trp
0.168TrpAla: 0.168 ± 0.151
0.0TrpCys: 0.0 ± 0.0
0.504TrpAsp: 0.504 ± 0.217
1.009TrpGlu: 1.009 ± 0.351
0.168TrpPhe: 0.168 ± 0.16
0.0TrpGly: 0.0 ± 0.0
0.168TrpHis: 0.168 ± 0.161
0.336TrpIle: 0.336 ± 0.321
0.841TrpLys: 0.841 ± 0.341
1.177TrpLeu: 1.177 ± 0.461
0.336TrpMet: 0.336 ± 0.218
0.168TrpAsn: 0.168 ± 0.16
0.0TrpPro: 0.0 ± 0.0
0.504TrpGln: 0.504 ± 0.281
0.336TrpArg: 0.336 ± 0.209
0.336TrpSer: 0.336 ± 0.247
0.336TrpThr: 0.336 ± 0.198
0.673TrpVal: 0.673 ± 0.381
0.0TrpTrp: 0.0 ± 0.0
0.504TrpTyr: 0.504 ± 0.241
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.513TyrAla: 1.513 ± 0.424
0.841TyrCys: 0.841 ± 0.397
3.027TyrAsp: 3.027 ± 0.608
5.381TyrGlu: 5.381 ± 1.084
3.867TyrPhe: 3.867 ± 0.849
4.036TyrGly: 4.036 ± 1.131
0.0TyrHis: 0.0 ± 0.0
2.69TyrIle: 2.69 ± 0.439
4.708TyrLys: 4.708 ± 0.555
3.531TyrLeu: 3.531 ± 0.643
1.177TyrMet: 1.177 ± 0.296
5.381TyrAsn: 5.381 ± 0.916
1.513TyrPro: 1.513 ± 0.561
1.513TyrGln: 1.513 ± 0.654
0.841TyrArg: 0.841 ± 0.344
2.859TyrSer: 2.859 ± 0.548
3.027TyrThr: 3.027 ± 0.875
2.018TyrVal: 2.018 ± 0.566
0.504TyrTrp: 0.504 ± 0.28
4.204TyrTyr: 4.204 ± 0.899
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 22 proteins (5948 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski