Aquimixticola soesokkakensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Aquimixticola

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3316 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1Y5T5N1|A0A1Y5T5N1_9RHOB Asp/Glu/Hydantoin racemase OS=Aquimixticola soesokkakensis OX=1519096 GN=AQS8620_02469 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.06ILVSSSVLALCATAAFADD20 pKa = 4.06CTEE23 pKa = 4.13VSFSDD28 pKa = 4.63VGWTDD33 pKa = 2.5ITTTTTIAKK42 pKa = 10.02QILEE46 pKa = 4.03PLGYY50 pKa = 10.17KK51 pKa = 10.05VDD53 pKa = 3.8VSVLSVPVTFASLSGGDD70 pKa = 3.29TDD72 pKa = 4.57IFLGNWMPAQSGAVTPYY89 pKa = 10.93LEE91 pKa = 5.4DD92 pKa = 3.43GTIEE96 pKa = 4.1SLHH99 pKa = 6.71EE100 pKa = 4.05NLQGTVYY107 pKa = 9.88TLAVPAYY114 pKa = 8.51TYY116 pKa = 11.04EE117 pKa = 4.55KK118 pKa = 10.34GLKK121 pKa = 10.1DD122 pKa = 3.59FADD125 pKa = 3.32IATFSDD131 pKa = 3.59ALDD134 pKa = 3.56DD135 pKa = 4.24TIYY138 pKa = 10.84GIEE141 pKa = 4.61PGNEE145 pKa = 3.73GNDD148 pKa = 3.48YY149 pKa = 10.87LISLTEE155 pKa = 4.34ADD157 pKa = 3.52THH159 pKa = 6.16GLGGFDD165 pKa = 3.28VKK167 pKa = 10.76EE168 pKa = 4.27SSEE171 pKa = 4.01QGMLSQVRR179 pKa = 11.84RR180 pKa = 11.84AYY182 pKa = 10.46DD183 pKa = 3.33KK184 pKa = 11.77NEE186 pKa = 4.86DD187 pKa = 3.47IVFLGWAPHH196 pKa = 5.62PMNVEE201 pKa = 3.86FDD203 pKa = 3.91LEE205 pKa = 4.32YY206 pKa = 10.43LTGGEE211 pKa = 4.42DD212 pKa = 3.52FFGGVGVVNTLTRR225 pKa = 11.84TGFAQEE231 pKa = 4.4CPNVGKK237 pKa = 10.59LLTNLDD243 pKa = 3.73FTVEE247 pKa = 4.08MEE249 pKa = 4.22SQIMGAILTDD259 pKa = 4.29GEE261 pKa = 4.54DD262 pKa = 3.44ADD264 pKa = 4.98DD265 pKa = 4.66AVSAYY270 pKa = 8.13ITANPGVLDD279 pKa = 3.44AWLEE283 pKa = 4.14GVTTLEE289 pKa = 4.4GDD291 pKa = 3.38AGLPAVKK298 pKa = 9.86AAYY301 pKa = 9.97GLL303 pKa = 3.86

Molecular weight:
32.24 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1Y5SZ26|A0A1Y5SZ26_9RHOB Methylated-DNA--[protein]-cysteine S-methyltransferase OS=Aquimixticola soesokkakensis OX=1519096 GN=ada PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.34SLSAA44 pKa = 3.83

Molecular weight:
5.13 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3316

0

3316

1038695

30

1526

313.2

33.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.89 ± 0.064

0.897 ± 0.011

6.07 ± 0.039

5.458 ± 0.043

3.786 ± 0.025

8.495 ± 0.04

1.976 ± 0.021

5.068 ± 0.029

3.282 ± 0.035

10.015 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.022

2.549 ± 0.02

4.956 ± 0.027

3.422 ± 0.026

6.41 ± 0.047

5.413 ± 0.035

5.697 ± 0.03

7.382 ± 0.035

1.295 ± 0.016

2.194 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski