Sporothrix insectorum RCEF 264

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Ophiostomatales; Ophiostomataceae; Sporothrix; Sporothrix insectorum

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9495 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A167WDZ3|A0A167WDZ3_9PEZI Uncharacterized protein OS=Sporothrix insectorum RCEF 264 OX=1081102 GN=SPI_03826 PE=4 SV=1
MM1 pKa = 7.77TDD3 pKa = 3.19NNNNNEE9 pKa = 4.23DD10 pKa = 4.33DD11 pKa = 4.66DD12 pKa = 5.88DD13 pKa = 4.15NTEE16 pKa = 4.03ATAEE20 pKa = 4.07DD21 pKa = 3.82NADD24 pKa = 4.14DD25 pKa = 4.89GEE27 pKa = 4.7CTPEE31 pKa = 4.36SPRR34 pKa = 11.84SDD36 pKa = 2.97QSDD39 pKa = 2.92IDD41 pKa = 3.7RR42 pKa = 11.84SNEE45 pKa = 3.48EE46 pKa = 4.78DD47 pKa = 3.64GPMYY51 pKa = 10.4YY52 pKa = 10.26CCQCKK57 pKa = 9.52AQKK60 pKa = 10.71EE61 pKa = 4.24EE62 pKa = 4.43TKK64 pKa = 10.85VKK66 pKa = 10.56EE67 pKa = 3.95DD68 pKa = 4.61DD69 pKa = 3.89GCSEE73 pKa = 4.78CGHH76 pKa = 6.01KK77 pKa = 10.18QCEE80 pKa = 4.36SCEE83 pKa = 4.17KK84 pKa = 10.63VDD86 pKa = 3.86

Molecular weight: 9.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A167WF13|A0A167WF13_9PEZI Uncharacterized protein OS=Sporothrix insectorum RCEF 264 OX=1081102 GN=SPI_03867 PE=4 SV=1
MM1 pKa = 7.44LAADD5 pKa = 4.1EE6 pKa = 4.66ARR8 pKa = 11.84IEE10 pKa = 3.93QLVRR14 pKa = 11.84DD15 pKa = 3.88EE16 pKa = 4.71RR17 pKa = 11.84EE18 pKa = 3.84LVGRR22 pKa = 11.84RR23 pKa = 11.84VFWQRR28 pKa = 11.84QRR30 pKa = 11.84QLNVVEE36 pKa = 4.57VEE38 pKa = 4.5RR39 pKa = 11.84SSSSYY44 pKa = 10.29PVLDD48 pKa = 3.87SFSSRR53 pKa = 11.84IMLVTSAAITSARR66 pKa = 11.84CSTKK70 pKa = 10.53GSGHH74 pKa = 5.83GLDD77 pKa = 3.16RR78 pKa = 11.84LSRR81 pKa = 11.84GVGRR85 pKa = 11.84CACPVQAEE93 pKa = 4.5QYY95 pKa = 8.84QQLPRR100 pKa = 11.84ASASARR106 pKa = 11.84ANVAFTKK113 pKa = 9.42STVRR117 pKa = 11.84RR118 pKa = 11.84SYY120 pKa = 11.07GHH122 pKa = 5.8SQSKK126 pKa = 9.11GQPATASTTLDD137 pKa = 3.56CCASDD142 pKa = 4.76GPQMPQVSRR151 pKa = 11.84DD152 pKa = 3.47VALSPHH158 pKa = 6.76HH159 pKa = 7.13DD160 pKa = 3.76GPRR163 pKa = 11.84HH164 pKa = 4.88GKK166 pKa = 8.3IAARR170 pKa = 11.84RR171 pKa = 11.84RR172 pKa = 11.84SNAAAVWKK180 pKa = 10.32AGHH183 pKa = 6.08AVSWYY188 pKa = 8.46QRR190 pKa = 11.84CRR192 pKa = 3.29

Molecular weight: 21.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9495

0

9495

5161090

51

5481

543.6

58.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.43 ± 0.03

1.107 ± 0.009

6.03 ± 0.019

5.189 ± 0.025

3.353 ± 0.014

7.851 ± 0.029

2.505 ± 0.012

3.546 ± 0.018

3.652 ± 0.024

8.56 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.869 ± 0.009

3.389 ± 0.013

6.541 ± 0.03

4.074 ± 0.026

6.683 ± 0.023

7.721 ± 0.035

6.195 ± 0.022

6.488 ± 0.021

1.315 ± 0.008

2.504 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski