Mycoplasma columbinasale

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A449B9L8|A0A449B9L8_9MOLU Uncharacterized protein OS=Mycoplasma columbinasale OX=114880 GN=NCTC10184_00067 PE=4 SV=1
MM1 pKa = 7.77ASNFLDD7 pKa = 4.08LHH9 pKa = 6.4GLDD12 pKa = 4.04QIQAISKK19 pKa = 9.32VVILLDD25 pKa = 3.19NALRR29 pKa = 11.84IKK31 pKa = 10.49IPVVHH36 pKa = 7.22IITGKK41 pKa = 10.61GNGALFTTALDD52 pKa = 3.9LLIKK56 pKa = 10.36EE57 pKa = 4.25NYY59 pKa = 8.57EE60 pKa = 3.83YY61 pKa = 10.85SIEE64 pKa = 4.15NQGGEE69 pKa = 4.17IIVFLQRR76 pKa = 11.84EE77 pKa = 4.63FKK79 pKa = 10.5QYY81 pKa = 11.12YY82 pKa = 9.49VDD84 pKa = 3.98DD85 pKa = 4.42DD86 pKa = 4.45EE87 pKa = 6.34YY88 pKa = 11.68YY89 pKa = 10.84FDD91 pKa = 5.2YY92 pKa = 11.27SDD94 pKa = 5.21DD95 pKa = 4.09GYY97 pKa = 11.89NVNDD101 pKa = 3.87LSKK104 pKa = 10.7KK105 pKa = 10.13YY106 pKa = 9.67EE107 pKa = 3.84

Molecular weight: 12.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A449BAD3|A0A449BAD3_9MOLU 50S ribosomal protein L19 OS=Mycoplasma columbinasale OX=114880 GN=rplS PE=3 SV=1
MM1 pKa = 7.67SLRR4 pKa = 11.84TYY6 pKa = 10.45QPNKK10 pKa = 8.2RR11 pKa = 11.84QHH13 pKa = 6.34AKK15 pKa = 7.64THH17 pKa = 5.42GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTANGRR29 pKa = 11.84KK30 pKa = 8.89VLAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.76GRR40 pKa = 11.84KK41 pKa = 8.92RR42 pKa = 11.84LTVSSKK48 pKa = 10.99

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

621

0

621

252187

29

3288

406.1

46.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.103 ± 0.135

0.476 ± 0.026

5.293 ± 0.076

6.276 ± 0.105

5.686 ± 0.085

4.71 ± 0.101

1.548 ± 0.042

7.308 ± 0.148

8.594 ± 0.134

10.081 ± 0.113

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.554 ± 0.051

6.985 ± 0.133

3.164 ± 0.081

4.274 ± 0.086

3.221 ± 0.058

6.02 ± 0.069

6.445 ± 0.121

6.369 ± 0.079

1.012 ± 0.032

3.881 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski