Amino acid dipepetide frequency for Mycoplasma columbinasale

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.119AlaAla: 5.119 ± 0.207
0.286AlaCys: 0.286 ± 0.031
3.716AlaAsp: 3.716 ± 0.173
4.191AlaGlu: 4.191 ± 0.149
3.604AlaPhe: 3.604 ± 0.151
3.307AlaGly: 3.307 ± 0.145
1.118AlaHis: 1.118 ± 0.068
4.814AlaIle: 4.814 ± 0.18
6.777AlaLys: 6.777 ± 0.16
7.007AlaLeu: 7.007 ± 0.218
1.102AlaMet: 1.102 ± 0.084
5.413AlaAsn: 5.413 ± 0.236
2.074AlaPro: 2.074 ± 0.123
3.977AlaGln: 3.977 ± 0.181
2.752AlaArg: 2.752 ± 0.118
3.755AlaSer: 3.755 ± 0.146
4.707AlaThr: 4.707 ± 0.192
4.164AlaVal: 4.164 ± 0.202
0.583AlaTrp: 0.583 ± 0.05
2.566AlaTyr: 2.566 ± 0.111
0.0AlaXaa: 0.0 ± 0.0
Cys
0.305CysAla: 0.305 ± 0.035
0.032CysCys: 0.032 ± 0.011
0.329CysAsp: 0.329 ± 0.044
0.313CysGlu: 0.313 ± 0.042
0.377CysPhe: 0.377 ± 0.045
0.389CysGly: 0.389 ± 0.041
0.075CysHis: 0.075 ± 0.019
0.222CysIle: 0.222 ± 0.03
0.286CysLys: 0.286 ± 0.037
0.456CysLeu: 0.456 ± 0.047
0.075CysMet: 0.075 ± 0.02
0.222CysAsn: 0.222 ± 0.029
0.171CysPro: 0.171 ± 0.024
0.238CysGln: 0.238 ± 0.038
0.163CysArg: 0.163 ± 0.024
0.325CysSer: 0.325 ± 0.041
0.297CysThr: 0.297 ± 0.041
0.274CysVal: 0.274 ± 0.038
0.044CysTrp: 0.044 ± 0.012
0.167CysTyr: 0.167 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
4.191AspAla: 4.191 ± 0.193
0.174AspCys: 0.174 ± 0.031
2.768AspAsp: 2.768 ± 0.122
3.858AspGlu: 3.858 ± 0.152
3.767AspPhe: 3.767 ± 0.152
2.855AspGly: 2.855 ± 0.142
0.837AspHis: 0.837 ± 0.059
3.248AspIle: 3.248 ± 0.137
4.084AspLys: 4.084 ± 0.143
5.619AspLeu: 5.619 ± 0.156
0.607AspMet: 0.607 ± 0.041
3.196AspAsn: 3.196 ± 0.123
1.868AspPro: 1.868 ± 0.09
2.332AspGln: 2.332 ± 0.11
1.495AspArg: 1.495 ± 0.086
2.915AspSer: 2.915 ± 0.103
2.72AspThr: 2.72 ± 0.102
3.367AspVal: 3.367 ± 0.126
0.531AspTrp: 0.531 ± 0.048
2.494AspTyr: 2.494 ± 0.128
0.0AspXaa: 0.0 ± 0.0
Glu
4.279GluAla: 4.279 ± 0.151
0.226GluCys: 0.226 ± 0.034
2.506GluAsp: 2.506 ± 0.165
3.878GluGlu: 3.878 ± 0.229
3.823GluPhe: 3.823 ± 0.159
2.185GluGly: 2.185 ± 0.109
0.979GluHis: 0.979 ± 0.061
5.393GluIle: 5.393 ± 0.165
5.607GluLys: 5.607 ± 0.217
7.213GluLeu: 7.213 ± 0.192
1.15GluMet: 1.15 ± 0.075
4.62GluAsn: 4.62 ± 0.155
1.626GluPro: 1.626 ± 0.112
2.938GluGln: 2.938 ± 0.108
2.205GluArg: 2.205 ± 0.127
3.097GluSer: 3.097 ± 0.122
4.072GluThr: 4.072 ± 0.155
4.029GluVal: 4.029 ± 0.132
0.575GluTrp: 0.575 ± 0.082
2.355GluTyr: 2.355 ± 0.108
0.0GluXaa: 0.0 ± 0.0
Phe
4.449PheAla: 4.449 ± 0.144
0.424PheCys: 0.424 ± 0.048
3.66PheAsp: 3.66 ± 0.137
3.624PheGlu: 3.624 ± 0.143
2.974PhePhe: 2.974 ± 0.158
3.053PheGly: 3.053 ± 0.116
0.678PheHis: 0.678 ± 0.052
3.684PheIle: 3.684 ± 0.143
4.616PheLys: 4.616 ± 0.127
5.821PheLeu: 5.821 ± 0.204
0.738PheMet: 0.738 ± 0.054
3.747PheAsn: 3.747 ± 0.158
1.412PhePro: 1.412 ± 0.074
1.721PheGln: 1.721 ± 0.087
1.622PheArg: 1.622 ± 0.086
3.973PheSer: 3.973 ± 0.151
3.557PheThr: 3.557 ± 0.141
4.31PheVal: 4.31 ± 0.185
0.67PheTrp: 0.67 ± 0.054
2.121PheTyr: 2.121 ± 0.101
0.0PheXaa: 0.0 ± 0.0
Gly
3.145GlyAla: 3.145 ± 0.125
0.238GlyCys: 0.238 ± 0.031
2.256GlyAsp: 2.256 ± 0.102
3.137GlyGlu: 3.137 ± 0.12
3.018GlyPhe: 3.018 ± 0.144
2.744GlyGly: 2.744 ± 0.131
0.868GlyHis: 0.868 ± 0.067
3.656GlyIle: 3.656 ± 0.162
4.152GlyLys: 4.152 ± 0.143
4.445GlyLeu: 4.445 ± 0.156
0.761GlyMet: 0.761 ± 0.056
2.332GlyAsn: 2.332 ± 0.109
0.876GlyPro: 0.876 ± 0.062
1.935GlyGln: 1.935 ± 0.081
1.574GlyArg: 1.574 ± 0.099
2.934GlySer: 2.934 ± 0.117
3.232GlyThr: 3.232 ± 0.142
3.287GlyVal: 3.287 ± 0.132
0.539GlyTrp: 0.539 ± 0.045
1.975GlyTyr: 1.975 ± 0.111
0.0GlyXaa: 0.0 ± 0.0
His
0.944HisAla: 0.944 ± 0.068
0.091HisCys: 0.091 ± 0.02
0.607HisAsp: 0.607 ± 0.049
0.821HisGlu: 0.821 ± 0.053
1.039HisPhe: 1.039 ± 0.078
0.837HisGly: 0.837 ± 0.057
0.254HisHis: 0.254 ± 0.035
1.324HisIle: 1.324 ± 0.076
1.447HisLys: 1.447 ± 0.083
1.57HisLeu: 1.57 ± 0.098
0.246HisMet: 0.246 ± 0.028
1.15HisAsn: 1.15 ± 0.073
0.575HisPro: 0.575 ± 0.052
0.694HisGln: 0.694 ± 0.05
0.535HisArg: 0.535 ± 0.053
0.924HisSer: 0.924 ± 0.059
0.972HisThr: 0.972 ± 0.065
0.535HisVal: 0.535 ± 0.053
0.19HisTrp: 0.19 ± 0.03
0.722HisTyr: 0.722 ± 0.056
0.0HisXaa: 0.0 ± 0.0
Ile
5.127IleAla: 5.127 ± 0.168
0.504IleCys: 0.504 ± 0.048
4.473IleAsp: 4.473 ± 0.169
4.453IleGlu: 4.453 ± 0.199
4.437IlePhe: 4.437 ± 0.178
3.668IleGly: 3.668 ± 0.146
0.908IleHis: 0.908 ± 0.067
5.401IleIle: 5.401 ± 0.212
6.356IleLys: 6.356 ± 0.213
6.963IleLeu: 6.963 ± 0.217
1.071IleMet: 1.071 ± 0.075
5.036IleAsn: 5.036 ± 0.15
2.221IlePro: 2.221 ± 0.107
2.502IleGln: 2.502 ± 0.099
2.141IleArg: 2.141 ± 0.101
4.81IleSer: 4.81 ± 0.185
4.719IleThr: 4.719 ± 0.181
4.762IleVal: 4.762 ± 0.158
0.638IleTrp: 0.638 ± 0.047
2.895IleTyr: 2.895 ± 0.114
0.0IleXaa: 0.0 ± 0.0
Lys
5.575LysAla: 5.575 ± 0.222
0.27LysCys: 0.27 ± 0.036
3.961LysAsp: 3.961 ± 0.14
5.988LysGlu: 5.988 ± 0.296
4.413LysPhe: 4.413 ± 0.152
2.934LysGly: 2.934 ± 0.145
1.523LysHis: 1.523 ± 0.084
7.447LysIle: 7.447 ± 0.254
7.316LysLys: 7.316 ± 0.273
8.521LysLeu: 8.521 ± 0.183
1.951LysMet: 1.951 ± 0.099
6.594LysAsn: 6.594 ± 0.197
2.597LysPro: 2.597 ± 0.13
3.632LysGln: 3.632 ± 0.129
2.466LysArg: 2.466 ± 0.112
4.354LysSer: 4.354 ± 0.145
6.055LysThr: 6.055 ± 0.171
5.912LysVal: 5.912 ± 0.186
0.896LysTrp: 0.896 ± 0.059
3.533LysTyr: 3.533 ± 0.133
0.0LysXaa: 0.0 ± 0.0
Leu
7.673LeuAla: 7.673 ± 0.201
0.587LeuCys: 0.587 ± 0.068
5.801LeuAsp: 5.801 ± 0.139
5.928LeuGlu: 5.928 ± 0.199
5.107LeuPhe: 5.107 ± 0.176
4.687LeuGly: 4.687 ± 0.144
1.511LeuHis: 1.511 ± 0.083
7.55LeuIle: 7.55 ± 0.214
8.724LeuLys: 8.724 ± 0.177
9.196LeuLeu: 9.196 ± 0.272
1.701LeuMet: 1.701 ± 0.082
7.201LeuAsn: 7.201 ± 0.19
3.831LeuPro: 3.831 ± 0.125
3.684LeuGln: 3.684 ± 0.139
3.156LeuArg: 3.156 ± 0.116
6.654LeuSer: 6.654 ± 0.198
7.146LeuThr: 7.146 ± 0.255
6.793LeuVal: 6.793 ± 0.166
0.908LeuTrp: 0.908 ± 0.058
2.978LeuTyr: 2.978 ± 0.12
0.0LeuXaa: 0.0 ± 0.0
Met
1.134MetAla: 1.134 ± 0.073
0.079MetCys: 0.079 ± 0.022
0.642MetAsp: 0.642 ± 0.055
0.745MetGlu: 0.745 ± 0.054
0.944MetPhe: 0.944 ± 0.063
0.686MetGly: 0.686 ± 0.049
0.341MetHis: 0.341 ± 0.036
1.106MetIle: 1.106 ± 0.069
1.404MetLys: 1.404 ± 0.07
1.63MetLeu: 1.63 ± 0.083
0.329MetMet: 0.329 ± 0.038
0.964MetAsn: 0.964 ± 0.063
0.71MetPro: 0.71 ± 0.057
0.761MetGln: 0.761 ± 0.056
0.551MetArg: 0.551 ± 0.046
1.209MetSer: 1.209 ± 0.07
0.789MetThr: 0.789 ± 0.049
0.793MetVal: 0.793 ± 0.063
0.194MetTrp: 0.194 ± 0.025
0.527MetTyr: 0.527 ± 0.048
0.0MetXaa: 0.0 ± 0.0
Asn
4.346AsnAla: 4.346 ± 0.207
0.317AsnCys: 0.317 ± 0.043
3.664AsnAsp: 3.664 ± 0.14
4.509AsnGlu: 4.509 ± 0.163
4.77AsnPhe: 4.77 ± 0.154
3.295AsnGly: 3.295 ± 0.116
1.019AsnHis: 1.019 ± 0.074
4.639AsnIle: 4.639 ± 0.166
5.825AsnLys: 5.825 ± 0.193
7.602AsnLeu: 7.602 ± 0.22
0.793AsnMet: 0.793 ± 0.055
5.012AsnAsn: 5.012 ± 0.196
2.443AsnPro: 2.443 ± 0.112
3.287AsnGln: 3.287 ± 0.147
2.157AsnArg: 2.157 ± 0.108
4.215AsnSer: 4.215 ± 0.161
3.838AsnThr: 3.838 ± 0.195
4.1AsnVal: 4.1 ± 0.146
0.853AsnTrp: 0.853 ± 0.064
3.164AsnTyr: 3.164 ± 0.123
0.0AsnXaa: 0.0 ± 0.0
Pro
2.451ProAla: 2.451 ± 0.141
0.071ProCys: 0.071 ± 0.017
1.705ProAsp: 1.705 ± 0.108
2.415ProGlu: 2.415 ± 0.122
1.499ProPhe: 1.499 ± 0.102
1.483ProGly: 1.483 ± 0.086
0.595ProHis: 0.595 ± 0.061
2.213ProIle: 2.213 ± 0.098
2.367ProLys: 2.367 ± 0.107
3.164ProLeu: 3.164 ± 0.142
0.46ProMet: 0.46 ± 0.048
2.117ProAsn: 2.117 ± 0.091
0.634ProPro: 0.634 ± 0.054
1.344ProGln: 1.344 ± 0.092
0.896ProArg: 0.896 ± 0.065
2.046ProSer: 2.046 ± 0.109
2.391ProThr: 2.391 ± 0.107
2.229ProVal: 2.229 ± 0.257
0.27ProTrp: 0.27 ± 0.029
1.289ProTyr: 1.289 ± 0.068
0.0ProXaa: 0.0 ± 0.0
Gln
3.549GlnAla: 3.549 ± 0.178
0.123GlnCys: 0.123 ± 0.021
2.034GlnAsp: 2.034 ± 0.079
2.3GlnGlu: 2.3 ± 0.083
1.646GlnPhe: 1.646 ± 0.083
1.733GlnGly: 1.733 ± 0.098
0.793GlnHis: 0.793 ± 0.058
3.438GlnIle: 3.438 ± 0.135
4.049GlnLys: 4.049 ± 0.142
4.195GlnLeu: 4.195 ± 0.168
0.821GlnMet: 0.821 ± 0.056
3.827GlnAsn: 3.827 ± 0.147
1.368GlnPro: 1.368 ± 0.1
2.046GlnGln: 2.046 ± 0.122
1.4GlnArg: 1.4 ± 0.077
2.054GlnSer: 2.054 ± 0.092
3.097GlnThr: 3.097 ± 0.14
2.435GlnVal: 2.435 ± 0.124
0.357GlnTrp: 0.357 ± 0.04
1.475GlnTyr: 1.475 ± 0.088
0.0GlnXaa: 0.0 ± 0.0
Arg
2.006ArgAla: 2.006 ± 0.097
0.147ArgCys: 0.147 ± 0.028
1.796ArgAsp: 1.796 ± 0.1
2.316ArgGlu: 2.316 ± 0.13
1.665ArgPhe: 1.665 ± 0.077
1.499ArgGly: 1.499 ± 0.082
0.492ArgHis: 0.492 ± 0.049
2.459ArgIle: 2.459 ± 0.122
3.014ArgLys: 3.014 ± 0.128
3.129ArgLeu: 3.129 ± 0.127
0.63ArgMet: 0.63 ± 0.051
2.133ArgAsn: 2.133 ± 0.085
0.991ArgPro: 0.991 ± 0.073
1.344ArgGln: 1.344 ± 0.069
1.297ArgArg: 1.297 ± 0.092
1.697ArgSer: 1.697 ± 0.088
1.872ArgThr: 1.872 ± 0.08
2.082ArgVal: 2.082 ± 0.089
0.297ArgTrp: 0.297 ± 0.034
1.348ArgTyr: 1.348 ± 0.077
0.0ArgXaa: 0.0 ± 0.0
Ser
4.429SerAla: 4.429 ± 0.193
0.369SerCys: 0.369 ± 0.037
3.394SerAsp: 3.394 ± 0.119
3.997SerGlu: 3.997 ± 0.123
3.676SerPhe: 3.676 ± 0.148
3.216SerGly: 3.216 ± 0.111
0.86SerHis: 0.86 ± 0.061
3.934SerIle: 3.934 ± 0.157
4.961SerLys: 4.961 ± 0.131
6.079SerLeu: 6.079 ± 0.184
0.757SerMet: 0.757 ± 0.049
3.716SerAsn: 3.716 ± 0.127
1.951SerPro: 1.951 ± 0.118
2.855SerGln: 2.855 ± 0.136
1.773SerArg: 1.773 ± 0.085
3.779SerSer: 3.779 ± 0.148
3.486SerThr: 3.486 ± 0.133
3.763SerVal: 3.763 ± 0.164
0.682SerTrp: 0.682 ± 0.056
2.526SerTyr: 2.526 ± 0.108
0.0SerXaa: 0.0 ± 0.0
Thr
4.021ThrAla: 4.021 ± 0.175
0.254ThrCys: 0.254 ± 0.037
3.148ThrAsp: 3.148 ± 0.135
2.982ThrGlu: 2.982 ± 0.116
3.854ThrPhe: 3.854 ± 0.138
3.148ThrGly: 3.148 ± 0.135
1.051ThrHis: 1.051 ± 0.065
4.846ThrIle: 4.846 ± 0.15
5.956ThrLys: 5.956 ± 0.169
6.412ThrLeu: 6.412 ± 0.204
0.757ThrMet: 0.757 ± 0.054
5.107ThrAsn: 5.107 ± 0.203
2.657ThrPro: 2.657 ± 0.125
2.792ThrGln: 2.792 ± 0.142
1.983ThrArg: 1.983 ± 0.095
4.128ThrSer: 4.128 ± 0.178
4.322ThrThr: 4.322 ± 0.186
3.692ThrVal: 3.692 ± 0.145
0.698ThrTrp: 0.698 ± 0.049
2.645ThrTyr: 2.645 ± 0.105
0.0ThrXaa: 0.0 ± 0.0
Val
4.945ValAla: 4.945 ± 0.165
0.389ValCys: 0.389 ± 0.045
3.624ValAsp: 3.624 ± 0.12
4.179ValGlu: 4.179 ± 0.171
3.196ValPhe: 3.196 ± 0.107
3.061ValGly: 3.061 ± 0.138
0.864ValHis: 0.864 ± 0.06
4.513ValIle: 4.513 ± 0.165
5.151ValLys: 5.151 ± 0.179
6.46ValLeu: 6.46 ± 0.165
0.833ValMet: 0.833 ± 0.054
4.088ValAsn: 4.088 ± 0.152
2.324ValPro: 2.324 ± 0.167
2.435ValGln: 2.435 ± 0.094
2.078ValArg: 2.078 ± 0.1
4.175ValSer: 4.175 ± 0.14
4.477ValThr: 4.477 ± 0.261
4.195ValVal: 4.195 ± 0.162
0.559ValTrp: 0.559 ± 0.048
2.145ValTyr: 2.145 ± 0.091
0.0ValXaa: 0.0 ± 0.0
Trp
0.69TrpAla: 0.69 ± 0.053
0.044TrpCys: 0.044 ± 0.016
0.504TrpAsp: 0.504 ± 0.046
0.65TrpGlu: 0.65 ± 0.056
0.611TrpPhe: 0.611 ± 0.047
0.333TrpGly: 0.333 ± 0.038
0.115TrpHis: 0.115 ± 0.027
0.825TrpIle: 0.825 ± 0.059
0.864TrpLys: 0.864 ± 0.057
0.983TrpLeu: 0.983 ± 0.061
0.186TrpMet: 0.186 ± 0.026
0.777TrpAsn: 0.777 ± 0.05
0.353TrpPro: 0.353 ± 0.086
0.381TrpGln: 0.381 ± 0.033
0.408TrpArg: 0.408 ± 0.04
0.595TrpSer: 0.595 ± 0.052
0.73TrpThr: 0.73 ± 0.05
0.583TrpVal: 0.583 ± 0.044
0.111TrpTrp: 0.111 ± 0.02
0.373TrpTyr: 0.373 ± 0.044
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.653TyrAla: 2.653 ± 0.108
0.135TyrCys: 0.135 ± 0.025
2.336TyrAsp: 2.336 ± 0.103
2.47TyrGlu: 2.47 ± 0.114
2.439TyrPhe: 2.439 ± 0.104
1.991TyrGly: 1.991 ± 0.097
0.595TyrHis: 0.595 ± 0.043
2.375TyrIle: 2.375 ± 0.104
2.986TyrLys: 2.986 ± 0.116
4.35TyrLeu: 4.35 ± 0.135
0.567TyrMet: 0.567 ± 0.047
2.597TyrAsn: 2.597 ± 0.092
0.96TyrPro: 0.96 ± 0.086
1.84TyrGln: 1.84 ± 0.095
1.562TyrArg: 1.562 ± 0.082
2.562TyrSer: 2.562 ± 0.093
1.975TyrThr: 1.975 ± 0.098
2.387TyrVal: 2.387 ± 0.086
0.519TyrTrp: 0.519 ± 0.044
1.515TyrTyr: 1.515 ± 0.082
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 621 proteins (252187 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski