Ascosphaera apis ARSEF 7405

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Ascosphaeraceae; Ascosphaera; Ascosphaera apis

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6442 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A167UZJ9|A0A167UZJ9_9EURO Acyl-CoA thioesterase OS=Ascosphaera apis ARSEF 7405 OX=392613 GN=AAP_06221 PE=3 SV=1
MM1 pKa = 7.07EE2 pKa = 4.91QDD4 pKa = 3.13NRR6 pKa = 11.84MITSDD11 pKa = 3.81VDD13 pKa = 3.43HH14 pKa = 7.5ADD16 pKa = 3.62DD17 pKa = 6.27DD18 pKa = 4.28IEE20 pKa = 5.36IDD22 pKa = 3.97IDD24 pKa = 4.16APQQDD29 pKa = 3.81GQGSYY34 pKa = 10.12NQDD37 pKa = 2.72VMIDD41 pKa = 4.25DD42 pKa = 4.63ISITSQPLEE51 pKa = 4.81DD52 pKa = 4.75ADD54 pKa = 4.1MMDD57 pKa = 3.68GDD59 pKa = 4.36EE60 pKa = 5.69QYY62 pKa = 10.04MEE64 pKa = 5.77DD65 pKa = 5.22DD66 pKa = 4.2INDD69 pKa = 3.66EE70 pKa = 3.98PMDD73 pKa = 3.97YY74 pKa = 11.13YY75 pKa = 11.11EE76 pKa = 4.94EE77 pKa = 6.1DD78 pKa = 3.31GDD80 pKa = 5.16AAIEE84 pKa = 4.19DD85 pKa = 3.92APEE88 pKa = 3.71EE89 pKa = 4.12HH90 pKa = 6.72NEE92 pKa = 3.98YY93 pKa = 10.15PEE95 pKa = 4.15EE96 pKa = 4.04EE97 pKa = 4.04MTIAEE102 pKa = 4.52DD103 pKa = 3.66MVEE106 pKa = 3.81EE107 pKa = 4.31PMQHH111 pKa = 6.07EE112 pKa = 4.6NVQEE116 pKa = 4.1VVPQSQPQSTPSTQQAAEE134 pKa = 4.25VAQPASSQPAAVEE147 pKa = 4.08PSVSATVNEE156 pKa = 4.33ISLEE160 pKa = 4.08QAPEE164 pKa = 4.14TPVAPDD170 pKa = 3.39LAEE173 pKa = 4.23KK174 pKa = 10.54SVEE177 pKa = 4.09NMSEE181 pKa = 4.07EE182 pKa = 4.37QVTSAVDD189 pKa = 3.16AQQPHH194 pKa = 7.12DD195 pKa = 3.98AQEE198 pKa = 4.13EE199 pKa = 4.96VPAPTEE205 pKa = 4.17NPSHH209 pKa = 6.75GEE211 pKa = 3.52ASVPVQDD218 pKa = 5.58DD219 pKa = 3.31AHH221 pKa = 7.2QEE223 pKa = 4.24GAQEE227 pKa = 4.03EE228 pKa = 5.07PEE230 pKa = 4.23TGVTAEE236 pKa = 3.69QHH238 pKa = 4.4TAEE241 pKa = 4.81NEE243 pKa = 4.13TEE245 pKa = 4.52GIQEE249 pKa = 4.25KK250 pKa = 9.62TEE252 pKa = 4.1HH253 pKa = 6.43TDD255 pKa = 3.07VANDD259 pKa = 3.33NKK261 pKa = 10.34QPEE264 pKa = 4.67SHH266 pKa = 6.97HH267 pKa = 6.43SPQAEE272 pKa = 3.82EE273 pKa = 4.0EE274 pKa = 4.39QPVEE278 pKa = 4.02GDD280 pKa = 3.61PKK282 pKa = 11.24NEE284 pKa = 3.72ATEE287 pKa = 4.38TQGHH291 pKa = 4.91QEE293 pKa = 4.07EE294 pKa = 4.7NPIVDD299 pKa = 3.76ASLALHH305 pKa = 5.5SVKK308 pKa = 10.52VIYY311 pKa = 9.9QDD313 pKa = 3.25NEE315 pKa = 3.88IYY317 pKa = 10.7LFPPPEE323 pKa = 5.11SDD325 pKa = 3.16ATDD328 pKa = 3.26TFFLHH333 pKa = 7.43DD334 pKa = 4.63EE335 pKa = 4.36SLAGQPICNLLEE347 pKa = 4.02ACRR350 pKa = 11.84GVLGDD355 pKa = 5.45HH356 pKa = 5.87ITEE359 pKa = 4.36DD360 pKa = 3.6EE361 pKa = 4.23EE362 pKa = 5.78LYY364 pKa = 11.0FDD366 pKa = 4.49IDD368 pKa = 3.51RR369 pKa = 11.84LGLSFIEE376 pKa = 5.02HH377 pKa = 6.87PSKK380 pKa = 10.63PYY382 pKa = 10.45TMSLYY387 pKa = 10.39QVVEE391 pKa = 4.33LYY393 pKa = 10.97LRR395 pKa = 11.84LCANDD400 pKa = 4.05EE401 pKa = 4.46DD402 pKa = 4.4NHH404 pKa = 6.47PEE406 pKa = 3.61PMYY409 pKa = 9.99ITLHH413 pKa = 5.8TKK415 pKa = 10.73LNTNAEE421 pKa = 4.2FANLVEE427 pKa = 4.6AARR430 pKa = 11.84SGKK433 pKa = 9.7GLSYY437 pKa = 10.91FEE439 pKa = 5.52AYY441 pKa = 10.08EE442 pKa = 4.28EE443 pKa = 4.14DD444 pKa = 3.61TGDD447 pKa = 3.96QEE449 pKa = 4.34SDD451 pKa = 2.98EE452 pKa = 4.39AAVEE456 pKa = 4.08QEE458 pKa = 3.92EE459 pKa = 4.23EE460 pKa = 4.56SYY462 pKa = 10.81IEE464 pKa = 3.97QGEE467 pKa = 4.24EE468 pKa = 3.59SAEE471 pKa = 3.75IYY473 pKa = 9.95EE474 pKa = 4.37AEE476 pKa = 4.18EE477 pKa = 4.41HH478 pKa = 6.53EE479 pKa = 4.66EE480 pKa = 3.91SHH482 pKa = 7.28DD483 pKa = 4.12AQGHH487 pKa = 6.62DD488 pKa = 4.19IGDD491 pKa = 3.4QEE493 pKa = 4.49TAQEE497 pKa = 4.11EE498 pKa = 4.38NTAQNADD505 pKa = 3.62PTTAPEE511 pKa = 4.26SDD513 pKa = 3.46QQQPAQDD520 pKa = 3.86ANQDD524 pKa = 3.92DD525 pKa = 5.02LEE527 pKa = 4.69HH528 pKa = 6.36ATHH531 pKa = 6.96EE532 pKa = 4.28NTADD536 pKa = 3.79VNDD539 pKa = 4.1KK540 pKa = 10.67PSAEE544 pKa = 4.25SEE546 pKa = 4.2FGSTTEE552 pKa = 4.25TYY554 pKa = 8.95EE555 pKa = 4.11HH556 pKa = 6.7EE557 pKa = 4.43ASEE560 pKa = 4.34EE561 pKa = 4.25LYY563 pKa = 11.1SDD565 pKa = 5.41LGNQTIAEE573 pKa = 4.18YY574 pKa = 10.47EE575 pKa = 4.61GYY577 pKa = 10.19NSQYY581 pKa = 11.32NEE583 pKa = 4.25MLDD586 pKa = 3.56THH588 pKa = 6.84HH589 pKa = 7.65DD590 pKa = 3.74KK591 pKa = 11.21PNEE594 pKa = 4.06DD595 pKa = 5.08DD596 pKa = 3.31ISAAAEE602 pKa = 4.04EE603 pKa = 4.75PVEE606 pKa = 4.1QTAVDD611 pKa = 4.25DD612 pKa = 4.64DD613 pKa = 5.05SNAAGHH619 pKa = 6.93DD620 pKa = 3.53EE621 pKa = 4.2HH622 pKa = 8.19ASLEE626 pKa = 4.49SVQRR630 pKa = 11.84EE631 pKa = 3.93PDD633 pKa = 3.3VTDD636 pKa = 3.68AQLEE640 pKa = 4.32PVAQGQEE647 pKa = 4.05PVPPAIHH654 pKa = 6.55TEE656 pKa = 4.08GLDD659 pKa = 3.66TRR661 pKa = 11.84SVDD664 pKa = 3.93ADD666 pKa = 3.65ANADD670 pKa = 3.63NYY672 pKa = 10.88EE673 pKa = 4.57DD674 pKa = 4.72APHH677 pKa = 6.95AYY679 pKa = 9.28EE680 pKa = 5.19AEE682 pKa = 4.28EE683 pKa = 3.98YY684 pKa = 10.94DD685 pKa = 5.25EE686 pKa = 5.96NGDD689 pKa = 4.61DD690 pKa = 4.61NDD692 pKa = 4.46EE693 pKa = 4.02LTEE696 pKa = 3.95AQRR699 pKa = 11.84IEE701 pKa = 3.95ARR703 pKa = 11.84EE704 pKa = 3.99SDD706 pKa = 3.79EE707 pKa = 3.86EE708 pKa = 4.11AQIGEE713 pKa = 4.1FDD715 pKa = 4.73DD716 pKa = 4.61EE717 pKa = 4.54YY718 pKa = 11.73KK719 pKa = 10.92EE720 pKa = 4.38ADD722 pKa = 3.36SDD724 pKa = 4.33NEE726 pKa = 4.09LEE728 pKa = 4.42EE729 pKa = 4.38PLEE732 pKa = 4.75GIDD735 pKa = 6.17DD736 pKa = 3.85VDD738 pKa = 3.47QAYY741 pKa = 9.9PMNDD745 pKa = 3.48EE746 pKa = 4.33EE747 pKa = 4.66TTATDD752 pKa = 3.29DD753 pKa = 4.04RR754 pKa = 11.84VFGEE758 pKa = 4.28AALHH762 pKa = 6.38GDD764 pKa = 3.44ADD766 pKa = 4.22EE767 pKa = 4.6EE768 pKa = 4.63LEE770 pKa = 4.57SGDD773 pKa = 4.02GVPAIDD779 pKa = 4.67DD780 pKa = 3.96GEE782 pKa = 4.95AEE784 pKa = 4.41SEE786 pKa = 4.36HH787 pKa = 7.18QFDD790 pKa = 4.67EE791 pKa = 4.46YY792 pKa = 11.43LGGDD796 pKa = 3.71TAAPAHH802 pKa = 6.2YY803 pKa = 9.7EE804 pKa = 4.09DD805 pKa = 5.42FVDD808 pKa = 5.94AVAQGTSGEE817 pKa = 4.28GTHH820 pKa = 6.92AFTEE824 pKa = 5.2EE825 pKa = 4.2YY826 pKa = 10.86AGGEE830 pKa = 4.34GEE832 pKa = 5.12AEE834 pKa = 4.08AEE836 pKa = 3.94ADD838 pKa = 3.93EE839 pKa = 5.0VDD841 pKa = 4.89DD842 pKa = 7.0DD843 pKa = 4.86EE844 pKa = 5.19FAQNEE849 pKa = 4.35LDD851 pKa = 4.01GDD853 pKa = 4.02DD854 pKa = 3.69AASVKK859 pKa = 10.28RR860 pKa = 11.84SRR862 pKa = 11.84DD863 pKa = 2.97EE864 pKa = 5.05DD865 pKa = 3.61EE866 pKa = 5.44ADD868 pKa = 3.13EE869 pKa = 4.87SEE871 pKa = 4.21AKK873 pKa = 9.86RR874 pKa = 11.84RR875 pKa = 11.84RR876 pKa = 11.84SS877 pKa = 3.19

Molecular weight: 96.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166PMV8|A0A166PMV8_9EURO Hydantoinase OS=Ascosphaera apis ARSEF 7405 OX=392613 GN=AAP_00151 PE=4 SV=1
MM1 pKa = 6.79EE2 pKa = 4.16TAGQKK7 pKa = 10.48SILPSIGKK15 pKa = 9.83ISRR18 pKa = 11.84LDD20 pKa = 3.14ACRR23 pKa = 11.84EE24 pKa = 4.18VTDD27 pKa = 5.11HH28 pKa = 7.06LDD30 pKa = 3.43KK31 pKa = 11.17YY32 pKa = 10.65PPIVWRR38 pKa = 11.84LEE40 pKa = 3.63RR41 pKa = 11.84RR42 pKa = 11.84TTDD45 pKa = 3.75LSEE48 pKa = 4.96DD49 pKa = 3.78YY50 pKa = 10.8QGGAGRR56 pKa = 11.84HH57 pKa = 4.21QRR59 pKa = 11.84PAKK62 pKa = 10.22RR63 pKa = 11.84PEE65 pKa = 3.93RR66 pKa = 11.84QNGQDD71 pKa = 3.19QSSNGGQQPQPGNNEE86 pKa = 3.49RR87 pKa = 11.84GYY89 pKa = 8.71YY90 pKa = 9.11QPSSGSFRR98 pKa = 11.84SRR100 pKa = 11.84PTRR103 pKa = 11.84RR104 pKa = 11.84EE105 pKa = 3.35GRR107 pKa = 11.84SRR109 pKa = 11.84EE110 pKa = 3.89RR111 pKa = 11.84RR112 pKa = 11.84ASRR115 pKa = 11.84AGAPLPMVIEE125 pKa = 4.3NSPVHH130 pKa = 6.26QYY132 pKa = 10.9DD133 pKa = 3.71SKK135 pKa = 11.06PIQPMSNSEE144 pKa = 3.85QGHH147 pKa = 6.25HH148 pKa = 5.81YY149 pKa = 10.08KK150 pKa = 10.03EE151 pKa = 4.67HH152 pKa = 5.83KK153 pKa = 9.19QQLIHH158 pKa = 5.35EE159 pKa = 4.76TEE161 pKa = 4.29PEE163 pKa = 4.19SRR165 pKa = 11.84GHH167 pKa = 5.25KK168 pKa = 9.17HH169 pKa = 6.53CSGKK173 pKa = 10.45SYY175 pKa = 10.79EE176 pKa = 4.27SAPEE180 pKa = 3.66KK181 pKa = 10.35VAPEE185 pKa = 4.0RR186 pKa = 11.84PVPTIRR192 pKa = 11.84EE193 pKa = 4.35YY194 pKa = 11.2YY195 pKa = 10.35GKK197 pKa = 8.3TPYY200 pKa = 10.09TPTVPGPCSQPQPRR214 pKa = 11.84FYY216 pKa = 10.77EE217 pKa = 4.15SHH219 pKa = 7.5DD220 pKa = 3.91EE221 pKa = 4.07NSFIPPVGLNTYY233 pKa = 10.87ADD235 pKa = 3.99RR236 pKa = 11.84QVPAPQPGLSAVIGGSEE253 pKa = 4.11VSSWPEE259 pKa = 3.74SYY261 pKa = 10.38EE262 pKa = 4.13GQHH265 pKa = 6.71PPDD268 pKa = 4.78LDD270 pKa = 3.9LEE272 pKa = 4.35IEE274 pKa = 4.27RR275 pKa = 11.84GRR277 pKa = 11.84DD278 pKa = 3.39HH279 pKa = 8.25QSMQRR284 pKa = 11.84NRR286 pKa = 11.84QQRR289 pKa = 11.84GRR291 pKa = 11.84DD292 pKa = 3.55GTGEE296 pKa = 4.11AGRR299 pKa = 11.84QTILPLRR306 pKa = 11.84PAEE309 pKa = 4.2PLPAQSIEE317 pKa = 4.32SPIEE321 pKa = 4.1RR322 pKa = 11.84PAHH325 pKa = 5.19RR326 pKa = 11.84PARR329 pKa = 11.84RR330 pKa = 11.84SFPRR334 pKa = 11.84HH335 pKa = 5.39EE336 pKa = 4.52GEE338 pKa = 4.18EE339 pKa = 4.24SRR341 pKa = 11.84EE342 pKa = 3.88QPAEE346 pKa = 4.07HH347 pKa = 6.52PAEE350 pKa = 4.03RR351 pKa = 11.84SSRR354 pKa = 11.84NKK356 pKa = 10.61DD357 pKa = 2.79KK358 pKa = 11.53AKK360 pKa = 10.21DD361 pKa = 3.2HH362 pKa = 6.31RR363 pKa = 11.84RR364 pKa = 11.84SHH366 pKa = 6.88RR367 pKa = 11.84EE368 pKa = 3.61VNEE371 pKa = 3.42SRR373 pKa = 11.84MRR375 pKa = 11.84RR376 pKa = 11.84RR377 pKa = 11.84EE378 pKa = 3.73RR379 pKa = 11.84NRR381 pKa = 11.84RR382 pKa = 11.84RR383 pKa = 11.84SLSRR387 pKa = 11.84LLGSHH392 pKa = 5.93SRR394 pKa = 11.84TRR396 pKa = 11.84DD397 pKa = 3.02RR398 pKa = 11.84ARR400 pKa = 11.84SQGRR404 pKa = 11.84KK405 pKa = 5.6QRR407 pKa = 11.84NRR409 pKa = 11.84RR410 pKa = 11.84SSGFFDD416 pKa = 4.05FMRR419 pKa = 11.84RR420 pKa = 11.84PSRR423 pKa = 11.84SGRR426 pKa = 11.84GSQSTSSSSSPNRR439 pKa = 11.84KK440 pKa = 9.69GKK442 pKa = 10.43DD443 pKa = 3.07SDD445 pKa = 4.5DD446 pKa = 3.73RR447 pKa = 11.84PSRR450 pKa = 11.84QGRR453 pKa = 11.84SRR455 pKa = 11.84SHH457 pKa = 5.07GRR459 pKa = 11.84GRR461 pKa = 11.84SRR463 pKa = 11.84SKK465 pKa = 9.37TRR467 pKa = 11.84SMPRR471 pKa = 11.84WSRR474 pKa = 11.84HH475 pKa = 3.91RR476 pKa = 11.84RR477 pKa = 11.84RR478 pKa = 11.84TSDD481 pKa = 3.99QEE483 pKa = 4.26EE484 pKa = 4.27EE485 pKa = 4.31WVDD488 pKa = 3.27VDD490 pKa = 5.02RR491 pKa = 11.84GDD493 pKa = 3.18GHH495 pKa = 6.1TSHH498 pKa = 6.89RR499 pKa = 11.84HH500 pKa = 3.98QRR502 pKa = 11.84RR503 pKa = 11.84TLSKK507 pKa = 7.52TQRR510 pKa = 11.84SSSRR514 pKa = 11.84CSRR517 pKa = 11.84HH518 pKa = 3.52QHH520 pKa = 4.85TGRR523 pKa = 11.84SRR525 pKa = 11.84SRR527 pKa = 11.84GQSASGDD534 pKa = 3.76CHH536 pKa = 7.2EE537 pKa = 4.8GDD539 pKa = 3.36RR540 pKa = 11.84WSDD543 pKa = 3.16SDD545 pKa = 4.02SQSHH549 pKa = 6.34FLPPKK554 pKa = 9.99EE555 pKa = 4.05VDD557 pKa = 3.25EE558 pKa = 4.48NAHH561 pKa = 6.22FKK563 pKa = 10.54ALKK566 pKa = 8.81KK567 pKa = 10.69LNVGRR572 pKa = 11.84HH573 pKa = 4.24RR574 pKa = 11.84RR575 pKa = 11.84KK576 pKa = 8.49EE577 pKa = 3.77QKK579 pKa = 9.87KK580 pKa = 9.65RR581 pKa = 11.84EE582 pKa = 4.06EE583 pKa = 3.9QCVICAATKK592 pKa = 10.51KK593 pKa = 10.36EE594 pKa = 4.07GFFEE598 pKa = 4.8GLFNHH603 pKa = 6.65TKK605 pKa = 10.81DD606 pKa = 3.45KK607 pKa = 10.9LASKK611 pKa = 10.3KK612 pKa = 10.22PRR614 pKa = 11.84DD615 pKa = 3.83GQQ617 pKa = 3.53

Molecular weight: 70.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6442

0

6442

3438916

50

4697

533.8

59.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.478 ± 0.028

1.116 ± 0.011

5.862 ± 0.023

6.516 ± 0.033

3.46 ± 0.019

6.447 ± 0.028

2.531 ± 0.015

4.896 ± 0.02

5.201 ± 0.029

8.317 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.181 ± 0.011

3.937 ± 0.017

6.205 ± 0.031

4.32 ± 0.028

6.22 ± 0.026

8.67 ± 0.042

6.053 ± 0.02

5.729 ± 0.021

1.199 ± 0.011

2.662 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski