Amino acid dipepetide frequency for Ascosphaera apis ARSEF 7405

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.905AlaAla: 9.905 ± 0.107	0.903AlaCys: 0.903 ± 0.02	4.139AlaAsp: 4.139 ± 0.037	5.248AlaGlu: 5.248 ± 0.055	2.786AlaPhe: 2.786 ± 0.03	5.568AlaGly: 5.568 ± 0.056	1.861AlaHis: 1.861 ± 0.027	4.032AlaIle: 4.032 ± 0.043	4.167AlaLys: 4.167 ± 0.04	6.89AlaLeu: 6.89 ± 0.056	1.868AlaMet: 1.868 ± 0.022	3.117AlaAsn: 3.117 ± 0.033	5.018AlaPro: 5.018 ± 0.058	3.612AlaGln: 3.612 ± 0.049	4.934AlaArg: 4.934 ± 0.04	7.493AlaSer: 7.493 ± 0.059	5.309AlaThr: 5.309 ± 0.044	4.996AlaVal: 4.996 ± 0.049	0.933AlaTrp: 0.933 ± 0.017	2.004AlaTyr: 2.004 ± 0.029	0.0AlaXaa: 0.0 ± 0.0
Cys	0.822CysAla: 0.822 ± 0.017	0.226CysCys: 0.226 ± 0.009	0.625CysAsp: 0.625 ± 0.013	0.599CysGlu: 0.599 ± 0.014	0.504CysPhe: 0.504 ± 0.013	0.81CysGly: 0.81 ± 0.017	0.297CysHis: 0.297 ± 0.01	0.628CysIle: 0.628 ± 0.013	0.49CysLys: 0.49 ± 0.012	1.154CysLeu: 1.154 ± 0.019	0.247CysMet: 0.247 ± 0.009	0.407CysAsn: 0.407 ± 0.01	0.624CysPro: 0.624 ± 0.016	0.405CysGln: 0.405 ± 0.012	0.669CysArg: 0.669 ± 0.014	0.847CysSer: 0.847 ± 0.019	0.585CysThr: 0.585 ± 0.015	0.742CysVal: 0.742 ± 0.015	0.155CysTrp: 0.155 ± 0.007	0.322CysTyr: 0.322 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	4.64AspAla: 4.64 ± 0.038	0.603AspCys: 0.603 ± 0.015	5.1AspAsp: 5.1 ± 0.075	5.009AspGlu: 5.009 ± 0.052	2.072AspPhe: 2.072 ± 0.027	3.876AspGly: 3.876 ± 0.038	1.307AspHis: 1.307 ± 0.026	3.383AspIle: 3.383 ± 0.036	2.628AspLys: 2.628 ± 0.035	4.799AspLeu: 4.799 ± 0.042	1.349AspMet: 1.349 ± 0.021	2.173AspAsn: 2.173 ± 0.025	3.187AspPro: 3.187 ± 0.033	1.895AspGln: 1.895 ± 0.021	3.125AspArg: 3.125 ± 0.039	4.287AspSer: 4.287 ± 0.043	3.1AspThr: 3.1 ± 0.031	3.702AspVal: 3.702 ± 0.034	0.764AspTrp: 0.764 ± 0.016	1.617AspTyr: 1.617 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	5.256GluAla: 5.256 ± 0.047	0.611GluCys: 0.611 ± 0.014	4.542GluAsp: 4.542 ± 0.051	6.561GluGlu: 6.561 ± 0.08	1.985GluPhe: 1.985 ± 0.024	3.825GluGly: 3.825 ± 0.041	1.507GluHis: 1.507 ± 0.024	3.238GluIle: 3.238 ± 0.031	4.46GluLys: 4.46 ± 0.052	5.155GluLeu: 5.155 ± 0.043	1.507GluMet: 1.507 ± 0.023	2.68GluAsn: 2.68 ± 0.03	2.709GluPro: 2.709 ± 0.033	2.783GluGln: 2.783 ± 0.035	4.294GluArg: 4.294 ± 0.057	4.406GluSer: 4.406 ± 0.044	3.617GluThr: 3.617 ± 0.034	3.534GluVal: 3.534 ± 0.037	0.778GluTrp: 0.778 ± 0.015	1.711GluTyr: 1.711 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	2.819PheAla: 2.819 ± 0.032	0.501PheCys: 0.501 ± 0.013	2.216PheAsp: 2.216 ± 0.028	2.127PheGlu: 2.127 ± 0.025	1.451PhePhe: 1.451 ± 0.028	2.519PheGly: 2.519 ± 0.033	0.841PheHis: 0.841 ± 0.015	1.72PheIle: 1.72 ± 0.025	1.613PheLys: 1.613 ± 0.023	3.045PheLeu: 3.045 ± 0.038	0.77PheMet: 0.77 ± 0.016	1.476PheAsn: 1.476 ± 0.02	1.799PhePro: 1.799 ± 0.022	1.252PheGln: 1.252 ± 0.02	1.828PheArg: 1.828 ± 0.026	2.881PheSer: 2.881 ± 0.031	2.071PheThr: 2.071 ± 0.024	2.118PheVal: 2.118 ± 0.028	0.507PheTrp: 0.507 ± 0.012	1.046PheTyr: 1.046 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	5.007GlyAla: 5.007 ± 0.055	0.767GlyCys: 0.767 ± 0.017	3.665GlyAsp: 3.665 ± 0.035	3.649GlyGlu: 3.649 ± 0.034	2.439GlyPhe: 2.439 ± 0.03	5.537GlyGly: 5.537 ± 0.078	1.716GlyHis: 1.716 ± 0.027	3.207GlyIle: 3.207 ± 0.032	3.468GlyLys: 3.468 ± 0.039	5.279GlyLeu: 5.279 ± 0.046	1.548GlyMet: 1.548 ± 0.028	2.705GlyAsn: 2.705 ± 0.031	3.111GlyPro: 3.111 ± 0.034	2.501GlyGln: 2.501 ± 0.03	3.878GlyArg: 3.878 ± 0.037	5.491GlySer: 5.491 ± 0.052	3.672GlyThr: 3.672 ± 0.034	4.01GlyVal: 4.01 ± 0.042	0.904GlyTrp: 0.904 ± 0.018	1.915GlyTyr: 1.915 ± 0.027	0.0GlyXaa: 0.0 ± 0.0
His	1.911HisAla: 1.911 ± 0.026	0.294HisCys: 0.294 ± 0.01	1.534HisAsp: 1.534 ± 0.024	1.466HisGlu: 1.466 ± 0.021	0.869HisPhe: 0.869 ± 0.016	1.679HisGly: 1.679 ± 0.023	1.302HisHis: 1.302 ± 0.036	1.317HisIle: 1.317 ± 0.022	0.994HisLys: 0.994 ± 0.02	2.183HisLeu: 2.183 ± 0.027	0.514HisMet: 0.514 ± 0.013	0.936HisAsn: 0.936 ± 0.018	1.778HisPro: 1.778 ± 0.029	1.165HisGln: 1.165 ± 0.024	1.61HisArg: 1.61 ± 0.024	2.006HisSer: 2.006 ± 0.029	1.402HisThr: 1.402 ± 0.021	1.367HisVal: 1.367 ± 0.023	0.29HisTrp: 0.29 ± 0.01	0.688HisTyr: 0.688 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.147IleAla: 4.147 ± 0.038	0.716IleCys: 0.716 ± 0.016	3.185IleAsp: 3.185 ± 0.032	3.161IleGlu: 3.161 ± 0.036	1.828IlePhe: 1.828 ± 0.024	2.955IleGly: 2.955 ± 0.041	1.196IleHis: 1.196 ± 0.02	2.523IleIle: 2.523 ± 0.03	2.36IleLys: 2.36 ± 0.027	4.2IleLeu: 4.2 ± 0.042	1.016IleMet: 1.016 ± 0.018	1.91IleAsn: 1.91 ± 0.024	3.16IlePro: 3.16 ± 0.031	1.846IleGln: 1.846 ± 0.023	2.839IleArg: 2.839 ± 0.029	4.018IleSer: 4.018 ± 0.033	2.863IleThr: 2.863 ± 0.029	3.019IleVal: 3.019 ± 0.033	0.609IleTrp: 0.609 ± 0.014	1.408IleTyr: 1.408 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	4.222LysAla: 4.222 ± 0.046	0.522LysCys: 0.522 ± 0.012	2.966LysAsp: 2.966 ± 0.036	4.096LysGlu: 4.096 ± 0.043	1.565LysPhe: 1.565 ± 0.026	3.053LysGly: 3.053 ± 0.034	1.227LysHis: 1.227 ± 0.018	2.528LysIle: 2.528 ± 0.029	4.14LysLys: 4.14 ± 0.057	4.162LysLeu: 4.162 ± 0.041	1.072LysMet: 1.072 ± 0.017	1.959LysAsn: 1.959 ± 0.027	2.89LysPro: 2.89 ± 0.034	2.139LysGln: 2.139 ± 0.029	3.795LysArg: 3.795 ± 0.042	3.774LysSer: 3.774 ± 0.042	3.067LysThr: 3.067 ± 0.035	2.769LysVal: 2.769 ± 0.027	0.616LysTrp: 0.616 ± 0.016	1.452LysTyr: 1.452 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	6.883LeuAla: 6.883 ± 0.057	1.055LeuCys: 1.055 ± 0.019	4.902LeuAsp: 4.902 ± 0.041	5.239LeuGlu: 5.239 ± 0.048	2.982LeuPhe: 2.982 ± 0.041	4.978LeuGly: 4.978 ± 0.043	2.154LeuHis: 2.154 ± 0.027	3.673LeuIle: 3.673 ± 0.042	4.467LeuLys: 4.467 ± 0.041	7.5LeuLeu: 7.5 ± 0.068	1.744LeuMet: 1.744 ± 0.025	3.368LeuAsn: 3.368 ± 0.031	5.287LeuPro: 5.287 ± 0.046	3.667LeuGln: 3.667 ± 0.04	5.658LeuArg: 5.658 ± 0.053	7.192LeuSer: 7.192 ± 0.047	4.646LeuThr: 4.646 ± 0.04	4.619LeuVal: 4.619 ± 0.041	0.949LeuTrp: 0.949 ± 0.02	2.209LeuTyr: 2.209 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	1.995MetAla: 1.995 ± 0.024	0.234MetCys: 0.234 ± 0.008	1.249MetAsp: 1.249 ± 0.019	1.296MetGlu: 1.296 ± 0.023	0.737MetPhe: 0.737 ± 0.016	1.443MetGly: 1.443 ± 0.024	0.486MetHis: 0.486 ± 0.011	1.026MetIle: 1.026 ± 0.017	1.144MetLys: 1.144 ± 0.019	1.78MetLeu: 1.78 ± 0.025	0.62MetMet: 0.62 ± 0.016	0.963MetAsn: 0.963 ± 0.018	1.336MetPro: 1.336 ± 0.024	0.888MetGln: 0.888 ± 0.018	1.314MetArg: 1.314 ± 0.021	2.074MetSer: 2.074 ± 0.028	1.308MetThr: 1.308 ± 0.019	1.161MetVal: 1.161 ± 0.019	0.236MetTrp: 0.236 ± 0.009	0.525MetTyr: 0.525 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.439AsnAla: 3.439 ± 0.036	0.429AsnCys: 0.429 ± 0.012	2.404AsnAsp: 2.404 ± 0.026	2.363AsnGlu: 2.363 ± 0.03	1.344AsnPhe: 1.344 ± 0.017	3.04AsnGly: 3.04 ± 0.041	0.983AsnHis: 0.983 ± 0.02	2.149AsnIle: 2.149 ± 0.024	1.78AsnLys: 1.78 ± 0.024	3.185AsnLeu: 3.185 ± 0.03	0.997AsnMet: 0.997 ± 0.02	1.92AsnAsn: 1.92 ± 0.029	2.518AsnPro: 2.518 ± 0.032	1.482AsnGln: 1.482 ± 0.024	2.103AsnArg: 2.103 ± 0.024	3.03AsnSer: 3.03 ± 0.032	2.266AsnThr: 2.266 ± 0.028	2.4AsnVal: 2.4 ± 0.028	0.484AsnTrp: 0.484 ± 0.011	1.06AsnTyr: 1.06 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	5.485ProAla: 5.485 ± 0.054	0.478ProCys: 0.478 ± 0.014	3.109ProAsp: 3.109 ± 0.033	3.942ProGlu: 3.942 ± 0.042	2.017ProPhe: 2.017 ± 0.027	3.684ProGly: 3.684 ± 0.042	1.47ProHis: 1.47 ± 0.026	2.685ProIle: 2.685 ± 0.028	2.729ProLys: 2.729 ± 0.033	4.748ProLeu: 4.748 ± 0.038	1.159ProMet: 1.159 ± 0.019	2.204ProAsn: 2.204 ± 0.026	5.793ProPro: 5.793 ± 0.092	2.762ProGln: 2.762 ± 0.043	3.401ProArg: 3.401 ± 0.04	6.567ProSer: 6.567 ± 0.058	4.075ProThr: 4.075 ± 0.036	3.63ProVal: 3.63 ± 0.039	0.597ProTrp: 0.597 ± 0.014	1.519ProTyr: 1.519 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	3.353GlnAla: 3.353 ± 0.044	0.418GlnCys: 0.418 ± 0.012	2.047GlnAsp: 2.047 ± 0.026	2.655GlnGlu: 2.655 ± 0.029	1.298GlnPhe: 1.298 ± 0.02	2.374GlnGly: 2.374 ± 0.03	1.253GlnHis: 1.253 ± 0.025	1.958GlnIle: 1.958 ± 0.025	2.216GlnLys: 2.216 ± 0.03	3.38GlnLeu: 3.38 ± 0.033	0.917GlnMet: 0.917 ± 0.017	1.712GlnAsn: 1.712 ± 0.023	2.851GlnPro: 2.851 ± 0.044	4.205GlnGln: 4.205 ± 0.102	2.858GlnArg: 2.858 ± 0.034	3.689GlnSer: 3.689 ± 0.039	2.417GlnThr: 2.417 ± 0.031	1.995GlnVal: 1.995 ± 0.024	0.47GlnTrp: 0.47 ± 0.012	1.133GlnTyr: 1.133 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	4.624ArgAla: 4.624 ± 0.041	0.712ArgCys: 0.712 ± 0.013	3.454ArgAsp: 3.454 ± 0.037	4.258ArgGlu: 4.258 ± 0.056	2.023ArgPhe: 2.023 ± 0.027	3.727ArgGly: 3.727 ± 0.041	1.707ArgHis: 1.707 ± 0.026	2.849ArgIle: 2.849 ± 0.03	3.783ArgLys: 3.783 ± 0.038	5.225ArgLeu: 5.225 ± 0.045	1.367ArgMet: 1.367 ± 0.02	2.404ArgAsn: 2.404 ± 0.028	3.566ArgPro: 3.566 ± 0.04	2.81ArgGln: 2.81 ± 0.031	5.543ArgArg: 5.543 ± 0.067	5.189ArgSer: 5.189 ± 0.054	3.31ArgThr: 3.31 ± 0.031	3.122ArgVal: 3.122 ± 0.03	0.829ArgTrp: 0.829 ± 0.015	1.696ArgTyr: 1.696 ± 0.025	0.0ArgXaa: 0.0 ± 0.0
Ser	7.166SerAla: 7.166 ± 0.062	0.792SerCys: 0.792 ± 0.018	4.451SerAsp: 4.451 ± 0.046	4.287SerGlu: 4.287 ± 0.041	2.896SerPhe: 2.896 ± 0.034	5.453SerGly: 5.453 ± 0.051	2.183SerHis: 2.183 ± 0.031	4.05SerIle: 4.05 ± 0.038	3.95SerLys: 3.95 ± 0.037	7.122SerLeu: 7.122 ± 0.052	1.793SerMet: 1.793 ± 0.022	3.304SerAsn: 3.304 ± 0.039	6.005SerPro: 6.005 ± 0.062	3.742SerGln: 3.742 ± 0.036	5.442SerArg: 5.442 ± 0.057	10.47SerSer: 10.47 ± 0.113	6.135SerThr: 6.135 ± 0.055	4.551SerVal: 4.551 ± 0.035	0.916SerTrp: 0.916 ± 0.018	1.992SerTyr: 1.992 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	5.502ThrAla: 5.502 ± 0.055	0.644ThrCys: 0.644 ± 0.016	2.914ThrAsp: 2.914 ± 0.029	3.195ThrGlu: 3.195 ± 0.033	2.1ThrPhe: 2.1 ± 0.024	3.978ThrGly: 3.978 ± 0.037	1.379ThrHis: 1.379 ± 0.023	2.998ThrIle: 2.998 ± 0.033	2.687ThrLys: 2.687 ± 0.031	4.908ThrLeu: 4.908 ± 0.041	1.159ThrMet: 1.159 ± 0.019	2.166ThrAsn: 2.166 ± 0.024	4.764ThrPro: 4.764 ± 0.044	2.225ThrGln: 2.225 ± 0.031	3.189ThrArg: 3.189 ± 0.038	5.922ThrSer: 5.922 ± 0.057	5.067ThrThr: 5.067 ± 0.062	3.537ThrVal: 3.537 ± 0.037	0.67ThrTrp: 0.67 ± 0.014	1.526ThrTyr: 1.526 ± 0.023	0.0ThrXaa: 0.0 ± 0.0
Val	4.655ValAla: 4.655 ± 0.042	0.729ValCys: 0.729 ± 0.015	3.649ValAsp: 3.649 ± 0.035	3.692ValGlu: 3.692 ± 0.037	2.175ValPhe: 2.175 ± 0.028	3.391ValGly: 3.391 ± 0.038	1.358ValHis: 1.358 ± 0.02	2.917ValIle: 2.917 ± 0.037	3.062ValLys: 3.062 ± 0.033	4.86ValLeu: 4.86 ± 0.039	1.238ValMet: 1.238 ± 0.02	2.298ValAsn: 2.298 ± 0.028	3.734ValPro: 3.734 ± 0.041	2.312ValGln: 2.312 ± 0.027	3.288ValArg: 3.288 ± 0.03	4.523ValSer: 4.523 ± 0.038	3.334ValThr: 3.334 ± 0.033	3.768ValVal: 3.768 ± 0.036	0.707ValTrp: 0.707 ± 0.014	1.604ValTyr: 1.604 ± 0.021	0.0ValXaa: 0.0 ± 0.0
Trp	0.89TrpAla: 0.89 ± 0.02	0.163TrpCys: 0.163 ± 0.008	0.758TrpAsp: 0.758 ± 0.017	0.749TrpGlu: 0.749 ± 0.016	0.45TrpPhe: 0.45 ± 0.012	0.713TrpGly: 0.713 ± 0.018	0.286TrpHis: 0.286 ± 0.009	0.639TrpIle: 0.639 ± 0.015	0.765TrpLys: 0.765 ± 0.015	1.103TrpLeu: 1.103 ± 0.02	0.305TrpMet: 0.305 ± 0.01	0.543TrpAsn: 0.543 ± 0.013	0.472TrpPro: 0.472 ± 0.012	0.465TrpGln: 0.465 ± 0.013	0.824TrpArg: 0.824 ± 0.017	0.881TrpSer: 0.881 ± 0.017	0.73TrpThr: 0.73 ± 0.015	0.692TrpVal: 0.692 ± 0.015	0.219TrpTrp: 0.219 ± 0.009	0.339TrpTyr: 0.339 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.063TyrAla: 2.063 ± 0.03	0.363TyrCys: 0.363 ± 0.01	1.71TyrAsp: 1.71 ± 0.022	1.569TyrGlu: 1.569 ± 0.026	1.082TyrPhe: 1.082 ± 0.019	1.864TyrGly: 1.864 ± 0.027	0.794TyrHis: 0.794 ± 0.017	1.439TyrIle: 1.439 ± 0.02	1.107TyrLys: 1.107 ± 0.019	2.493TyrLeu: 2.493 ± 0.03	0.622TyrMet: 0.622 ± 0.013	1.128TyrAsn: 1.128 ± 0.021	1.452TyrPro: 1.452 ± 0.02	1.042TyrGln: 1.042 ± 0.017	1.609TyrArg: 1.609 ± 0.025	1.959TyrSer: 1.959 ± 0.025	1.555TyrThr: 1.555 ± 0.024	1.561TyrVal: 1.561 ± 0.022	0.353TyrTrp: 0.353 ± 0.01	0.854TyrTyr: 0.854 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6442 proteins (3438916 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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