Pyrobaculum spherical virus 2

Taxonomy: Viruses; Globuloviridae; Alphaglobulovirus; Alphaglobulovirus PSV2

Average proteome isoelectric point is 7.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3VY50|A0A6M3VY50_9VIRU Virus coat protein VP2 OS=Pyrobaculum spherical virus 2 OX=2730632 GN=PSV2_gp14 PE=4 SV=1
MM1 pKa = 7.6SKK3 pKa = 10.5YY4 pKa = 10.33LEE6 pKa = 4.17KK7 pKa = 10.74ALYY10 pKa = 10.19VLALVAALSGIFGFVSQASAALFAALLLAIGEE42 pKa = 4.61LIEE45 pKa = 4.97DD46 pKa = 3.86

Molecular weight: 4.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3VX77|A0A6M3VX77_9VIRU Uncharacterized protein OS=Pyrobaculum spherical virus 2 OX=2730632 GN=PSV2_gp16 PE=4 SV=1
MM1 pKa = 7.85PKK3 pKa = 10.46NLTNRR8 pKa = 11.84VKK10 pKa = 10.39QWIIVLARR18 pKa = 11.84LDD20 pKa = 3.81RR21 pKa = 11.84LITLTGVARR30 pKa = 11.84QHH32 pKa = 6.38NIPHH36 pKa = 5.7STAHH40 pKa = 6.15HH41 pKa = 6.55LVRR44 pKa = 11.84LLKK47 pKa = 10.83GIIEE51 pKa = 3.95ARR53 pKa = 11.84KK54 pKa = 8.65IGGVIYY60 pKa = 10.11IAPYY64 pKa = 10.83GKK66 pKa = 9.72FDD68 pKa = 3.77EE69 pKa = 5.52YY70 pKa = 11.04AAQWGKK76 pKa = 8.99WFYY79 pKa = 11.42EE80 pKa = 3.94MAVRR84 pKa = 11.84LLNGCRR90 pKa = 11.84SQCCRR95 pKa = 11.84VPISTVYY102 pKa = 10.11KK103 pKa = 9.76YY104 pKa = 11.24YY105 pKa = 8.72RR106 pKa = 11.84TKK108 pKa = 10.15IIARR112 pKa = 11.84ARR114 pKa = 11.84EE115 pKa = 4.02EE116 pKa = 4.36LGLLRR121 pKa = 11.84PIGITAFIYY130 pKa = 10.76YY131 pKa = 9.5SVKK134 pKa = 10.4AYY136 pKa = 9.94KK137 pKa = 9.99EE138 pKa = 3.78DD139 pKa = 3.33AAEE142 pKa = 4.63IISEE146 pKa = 4.14KK147 pKa = 10.68PFIKK151 pKa = 10.04VCRR154 pKa = 11.84GKK156 pKa = 10.5AHH158 pKa = 6.03NN159 pKa = 4.16

Molecular weight: 18.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32

0

32

5548

46

525

173.4

19.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.435 ± 0.566

1.298 ± 0.214

3.371 ± 0.365

4.488 ± 0.67

4.056 ± 0.364

6.921 ± 0.599

1.658 ± 0.265

8.652 ± 0.359

5.047 ± 0.544

10.959 ± 0.99

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.145 ± 0.223

3.371 ± 0.39

4.867 ± 0.264

2.271 ± 0.236

4.65 ± 0.449

5.858 ± 0.391

5.894 ± 0.536

8.85 ± 0.452

1.442 ± 0.156

5.768 ± 0.367
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski