Amino acid dipepetide frequency for Pyrobaculum spherical virus 2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.031AlaAla: 7.031 ± 1.27
0.901AlaCys: 0.901 ± 0.329
2.704AlaAsp: 2.704 ± 0.779
3.425AlaGlu: 3.425 ± 1.175
3.786AlaPhe: 3.786 ± 0.948
2.884AlaGly: 2.884 ± 0.743
1.262AlaHis: 1.262 ± 0.453
9.915AlaIle: 9.915 ± 1.973
4.327AlaLys: 4.327 ± 1.003
13.521AlaLeu: 13.521 ± 2.717
1.622AlaMet: 1.622 ± 0.624
1.983AlaAsn: 1.983 ± 0.546
2.344AlaPro: 2.344 ± 0.663
1.983AlaGln: 1.983 ± 0.595
3.065AlaArg: 3.065 ± 0.891
5.589AlaSer: 5.589 ± 1.043
4.507AlaThr: 4.507 ± 1.149
8.473AlaVal: 8.473 ± 1.334
1.262AlaTrp: 1.262 ± 0.513
3.606AlaTyr: 3.606 ± 0.759
0.0AlaXaa: 0.0 ± 0.0
Cys
0.901CysAla: 0.901 ± 0.382
0.18CysCys: 0.18 ± 0.158
0.901CysAsp: 0.901 ± 0.436
0.721CysGlu: 0.721 ± 0.375
0.18CysPhe: 0.18 ± 0.176
1.262CysGly: 1.262 ± 0.519
0.18CysHis: 0.18 ± 0.181
0.541CysIle: 0.541 ± 0.317
0.721CysLys: 0.721 ± 0.421
0.901CysLeu: 0.901 ± 0.415
0.18CysMet: 0.18 ± 0.168
0.721CysAsn: 0.721 ± 0.391
0.901CysPro: 0.901 ± 0.425
0.18CysGln: 0.18 ± 0.168
1.082CysArg: 1.082 ± 0.523
0.901CysSer: 0.901 ± 0.48
0.18CysThr: 0.18 ± 0.176
1.622CysVal: 1.622 ± 0.572
0.18CysTrp: 0.18 ± 0.168
0.541CysTyr: 0.541 ± 0.278
0.0CysXaa: 0.0 ± 0.0
Asp
3.065AspAla: 3.065 ± 0.74
0.361AspCys: 0.361 ± 0.224
0.541AspAsp: 0.541 ± 0.384
1.082AspGlu: 1.082 ± 0.509
1.983AspPhe: 1.983 ± 0.528
1.803AspGly: 1.803 ± 0.581
0.0AspHis: 0.0 ± 0.0
3.245AspIle: 3.245 ± 0.732
2.524AspLys: 2.524 ± 0.86
1.983AspLeu: 1.983 ± 0.637
1.082AspMet: 1.082 ± 0.409
1.082AspAsn: 1.082 ± 0.469
2.344AspPro: 2.344 ± 0.793
0.541AspGln: 0.541 ± 0.299
1.442AspArg: 1.442 ± 0.498
1.082AspSer: 1.082 ± 0.483
2.704AspThr: 2.704 ± 0.822
4.687AspVal: 4.687 ± 0.965
0.18AspTrp: 0.18 ± 0.188
1.983AspTyr: 1.983 ± 0.503
0.0AspXaa: 0.0 ± 0.0
Glu
5.048GluAla: 5.048 ± 1.563
1.442GluCys: 1.442 ± 0.563
1.803GluAsp: 1.803 ± 0.819
5.408GluGlu: 5.408 ± 1.066
0.721GluPhe: 0.721 ± 0.309
1.983GluGly: 1.983 ± 0.698
0.18GluHis: 0.18 ± 0.193
4.146GluIle: 4.146 ± 0.938
3.425GluLys: 3.425 ± 0.903
3.786GluLeu: 3.786 ± 0.784
1.622GluMet: 1.622 ± 0.525
0.721GluAsn: 0.721 ± 0.536
0.721GluPro: 0.721 ± 0.357
0.721GluGln: 0.721 ± 0.384
1.082GluArg: 1.082 ± 0.583
1.803GluSer: 1.803 ± 0.668
2.524GluThr: 2.524 ± 0.733
3.966GluVal: 3.966 ± 0.705
0.901GluTrp: 0.901 ± 0.52
2.884GluTyr: 2.884 ± 0.735
0.0GluXaa: 0.0 ± 0.0
Phe
2.163PheAla: 2.163 ± 0.467
0.0PheCys: 0.0 ± 0.0
2.163PheAsp: 2.163 ± 0.716
0.721PheGlu: 0.721 ± 0.411
2.704PhePhe: 2.704 ± 0.942
3.245PheGly: 3.245 ± 0.829
0.541PheHis: 0.541 ± 0.29
2.884PheIle: 2.884 ± 0.813
1.983PheLys: 1.983 ± 0.529
4.687PheLeu: 4.687 ± 1.207
1.262PheMet: 1.262 ± 0.401
1.803PheAsn: 1.803 ± 0.417
1.983PhePro: 1.983 ± 0.95
0.901PheGln: 0.901 ± 0.356
2.704PheArg: 2.704 ± 0.593
2.704PheSer: 2.704 ± 0.579
2.344PheThr: 2.344 ± 0.782
3.425PheVal: 3.425 ± 0.731
0.18PheTrp: 0.18 ± 0.165
2.163PheTyr: 2.163 ± 0.59
0.0PheXaa: 0.0 ± 0.0
Gly
3.245GlyAla: 3.245 ± 0.871
0.361GlyCys: 0.361 ± 0.256
3.245GlyAsp: 3.245 ± 0.623
3.425GlyGlu: 3.425 ± 0.768
3.966GlyPhe: 3.966 ± 0.664
6.49GlyGly: 6.49 ± 2.515
0.721GlyHis: 0.721 ± 0.33
6.31GlyIle: 6.31 ± 1.412
2.884GlyLys: 2.884 ± 0.974
6.31GlyLeu: 6.31 ± 1.23
1.082GlyMet: 1.082 ± 0.474
2.344GlyAsn: 2.344 ± 0.58
1.622GlyPro: 1.622 ± 0.422
1.983GlyGln: 1.983 ± 0.651
2.704GlyArg: 2.704 ± 1.06
4.507GlySer: 4.507 ± 1.056
4.327GlyThr: 4.327 ± 1.228
7.211GlyVal: 7.211 ± 1.367
1.262GlyTrp: 1.262 ± 0.577
5.228GlyTyr: 5.228 ± 0.829
0.0GlyXaa: 0.0 ± 0.0
His
1.622HisAla: 1.622 ± 0.451
0.0HisCys: 0.0 ± 0.0
0.721HisAsp: 0.721 ± 0.323
0.541HisGlu: 0.541 ± 0.301
0.901HisPhe: 0.901 ± 0.489
0.361HisGly: 0.361 ± 0.249
0.721HisHis: 0.721 ± 0.335
1.622HisIle: 1.622 ± 0.585
1.262HisLys: 1.262 ± 0.52
1.442HisLeu: 1.442 ± 0.58
0.541HisMet: 0.541 ± 0.323
0.721HisAsn: 0.721 ± 0.402
0.721HisPro: 0.721 ± 0.384
0.18HisGln: 0.18 ± 0.202
0.901HisArg: 0.901 ± 0.512
0.901HisSer: 0.901 ± 0.476
0.541HisThr: 0.541 ± 0.39
1.622HisVal: 1.622 ± 0.595
0.0HisTrp: 0.0 ± 0.0
1.262HisTyr: 1.262 ± 0.37
0.0HisXaa: 0.0 ± 0.0
Ile
8.473IleAla: 8.473 ± 1.429
1.803IleCys: 1.803 ± 0.652
3.065IleAsp: 3.065 ± 0.778
4.146IleGlu: 4.146 ± 0.86
3.786IlePhe: 3.786 ± 0.669
6.67IleGly: 6.67 ± 1.1
1.442IleHis: 1.442 ± 0.393
7.572IleIle: 7.572 ± 1.208
2.704IleLys: 2.704 ± 0.714
6.851IleLeu: 6.851 ± 1.131
1.442IleMet: 1.442 ± 0.474
3.065IleAsn: 3.065 ± 0.736
6.851IlePro: 6.851 ± 1.148
1.442IleGln: 1.442 ± 0.458
3.606IleArg: 3.606 ± 0.99
3.966IleSer: 3.966 ± 0.781
6.31IleThr: 6.31 ± 1.055
7.031IleVal: 7.031 ± 1.365
1.082IleTrp: 1.082 ± 0.374
5.228IleTyr: 5.228 ± 0.709
0.0IleXaa: 0.0 ± 0.0
Lys
4.327LysAla: 4.327 ± 1.004
0.18LysCys: 0.18 ± 0.197
2.344LysAsp: 2.344 ± 0.687
2.704LysGlu: 2.704 ± 1.006
2.163LysPhe: 2.163 ± 0.652
3.606LysGly: 3.606 ± 0.825
1.622LysHis: 1.622 ± 0.542
3.245LysIle: 3.245 ± 0.691
1.803LysLys: 1.803 ± 0.744
4.867LysLeu: 4.867 ± 1.001
1.622LysMet: 1.622 ± 0.55
2.163LysAsn: 2.163 ± 0.757
2.704LysPro: 2.704 ± 0.676
0.901LysGln: 0.901 ± 0.416
3.245LysArg: 3.245 ± 0.695
2.344LysSer: 2.344 ± 0.604
1.803LysThr: 1.803 ± 0.726
4.867LysVal: 4.867 ± 1.233
1.082LysTrp: 1.082 ± 0.4
2.884LysTyr: 2.884 ± 0.605
0.0LysXaa: 0.0 ± 0.0
Leu
9.374LeuAla: 9.374 ± 1.817
1.262LeuCys: 1.262 ± 0.442
3.966LeuAsp: 3.966 ± 0.711
4.146LeuGlu: 4.146 ± 0.983
4.327LeuPhe: 4.327 ± 1.02
8.473LeuGly: 8.473 ± 2.093
1.262LeuHis: 1.262 ± 0.521
9.915LeuIle: 9.915 ± 1.318
5.949LeuLys: 5.949 ± 1.136
14.242LeuLeu: 14.242 ± 2.486
1.803LeuMet: 1.803 ± 0.527
2.524LeuAsn: 2.524 ± 0.663
5.228LeuPro: 5.228 ± 1.146
1.983LeuGln: 1.983 ± 0.542
5.408LeuArg: 5.408 ± 0.804
8.473LeuSer: 8.473 ± 1.312
6.31LeuThr: 6.31 ± 1.036
7.932LeuVal: 7.932 ± 1.065
1.803LeuTrp: 1.803 ± 0.519
5.228LeuTyr: 5.228 ± 0.803
0.0LeuXaa: 0.0 ± 0.0
Met
3.245MetAla: 3.245 ± 0.84
0.18MetCys: 0.18 ± 0.16
0.361MetAsp: 0.361 ± 0.257
0.18MetGlu: 0.18 ± 0.202
0.18MetPhe: 0.18 ± 0.168
1.803MetGly: 1.803 ± 0.526
0.18MetHis: 0.18 ± 0.179
1.262MetIle: 1.262 ± 0.669
1.082MetLys: 1.082 ± 0.454
1.983MetLeu: 1.983 ± 0.699
0.18MetMet: 0.18 ± 0.204
0.721MetAsn: 0.721 ± 0.304
2.344MetPro: 2.344 ± 0.587
0.361MetGln: 0.361 ± 0.255
2.344MetArg: 2.344 ± 0.618
1.983MetSer: 1.983 ± 0.57
0.541MetThr: 0.541 ± 0.312
0.901MetVal: 0.901 ± 0.5
0.901MetTrp: 0.901 ± 0.455
0.721MetTyr: 0.721 ± 0.325
0.0MetXaa: 0.0 ± 0.0
Asn
3.425AsnAla: 3.425 ± 0.601
0.901AsnCys: 0.901 ± 0.416
0.541AsnAsp: 0.541 ± 0.362
1.262AsnGlu: 1.262 ± 0.46
1.442AsnPhe: 1.442 ± 0.521
4.687AsnGly: 4.687 ± 1.317
0.361AsnHis: 0.361 ± 0.246
1.803AsnIle: 1.803 ± 0.431
0.721AsnLys: 0.721 ± 0.306
3.606AsnLeu: 3.606 ± 0.78
0.901AsnMet: 0.901 ± 0.343
1.262AsnAsn: 1.262 ± 0.365
0.901AsnPro: 0.901 ± 0.533
0.541AsnGln: 0.541 ± 0.299
1.622AsnArg: 1.622 ± 0.612
1.442AsnSer: 1.442 ± 0.484
2.524AsnThr: 2.524 ± 0.949
4.327AsnVal: 4.327 ± 1.01
0.541AsnTrp: 0.541 ± 0.263
0.901AsnTyr: 0.901 ± 0.408
0.0AsnXaa: 0.0 ± 0.0
Pro
3.245ProAla: 3.245 ± 0.812
0.541ProCys: 0.541 ± 0.276
1.082ProAsp: 1.082 ± 0.362
1.983ProGlu: 1.983 ± 0.74
2.163ProPhe: 2.163 ± 0.587
2.524ProGly: 2.524 ± 0.579
0.901ProHis: 0.901 ± 0.358
6.129ProIle: 6.129 ± 0.978
2.704ProLys: 2.704 ± 0.744
5.228ProLeu: 5.228 ± 1.189
1.262ProMet: 1.262 ± 0.512
1.803ProAsn: 1.803 ± 0.599
2.884ProPro: 2.884 ± 0.633
2.884ProGln: 2.884 ± 0.603
1.803ProArg: 1.803 ± 0.442
2.884ProSer: 2.884 ± 0.684
1.983ProThr: 1.983 ± 0.656
3.606ProVal: 3.606 ± 0.685
0.361ProTrp: 0.361 ± 0.226
2.704ProTyr: 2.704 ± 0.736
0.0ProXaa: 0.0 ± 0.0
Gln
1.442GlnAla: 1.442 ± 0.553
0.721GlnCys: 0.721 ± 0.356
1.082GlnAsp: 1.082 ± 0.397
0.901GlnGlu: 0.901 ± 0.481
2.344GlnPhe: 2.344 ± 0.609
1.442GlnGly: 1.442 ± 0.578
0.361GlnHis: 0.361 ± 0.255
1.983GlnIle: 1.983 ± 0.524
0.361GlnLys: 0.361 ± 0.229
3.245GlnLeu: 3.245 ± 0.748
0.901GlnMet: 0.901 ± 0.416
0.18GlnAsn: 0.18 ± 0.168
1.442GlnPro: 1.442 ± 0.677
0.541GlnGln: 0.541 ± 0.311
1.262GlnArg: 1.262 ± 0.416
0.361GlnSer: 0.361 ± 0.352
1.082GlnThr: 1.082 ± 0.385
0.721GlnVal: 0.721 ± 0.36
1.262GlnTrp: 1.262 ± 0.487
1.082GlnTyr: 1.082 ± 0.388
0.0GlnXaa: 0.0 ± 0.0
Arg
3.245ArgAla: 3.245 ± 0.798
0.541ArgCys: 0.541 ± 0.276
1.262ArgAsp: 1.262 ± 0.383
3.245ArgGlu: 3.245 ± 0.838
1.442ArgPhe: 1.442 ± 0.566
2.524ArgGly: 2.524 ± 0.593
1.082ArgHis: 1.082 ± 0.519
3.606ArgIle: 3.606 ± 0.875
3.606ArgLys: 3.606 ± 0.809
6.67ArgLeu: 6.67 ± 1.293
0.901ArgMet: 0.901 ± 0.338
1.622ArgAsn: 1.622 ± 0.552
1.803ArgPro: 1.803 ± 0.629
0.901ArgGln: 0.901 ± 0.422
3.966ArgArg: 3.966 ± 0.776
2.344ArgSer: 2.344 ± 0.599
1.262ArgThr: 1.262 ± 0.485
3.606ArgVal: 3.606 ± 1.016
0.721ArgTrp: 0.721 ± 0.322
2.163ArgTyr: 2.163 ± 0.653
0.0ArgXaa: 0.0 ± 0.0
Ser
5.769SerAla: 5.769 ± 0.724
0.901SerCys: 0.901 ± 0.377
1.622SerAsp: 1.622 ± 0.513
3.245SerGlu: 3.245 ± 0.706
1.442SerPhe: 1.442 ± 0.404
6.31SerGly: 6.31 ± 1.189
1.082SerHis: 1.082 ± 0.411
3.966SerIle: 3.966 ± 0.851
1.803SerLys: 1.803 ± 0.542
7.211SerLeu: 7.211 ± 1.204
1.442SerMet: 1.442 ± 0.584
2.344SerAsn: 2.344 ± 0.634
3.245SerPro: 3.245 ± 0.622
1.803SerGln: 1.803 ± 0.471
2.524SerArg: 2.524 ± 0.796
3.065SerSer: 3.065 ± 0.745
4.146SerThr: 4.146 ± 0.891
3.606SerVal: 3.606 ± 0.766
0.721SerTrp: 0.721 ± 0.286
2.344SerTyr: 2.344 ± 0.704
0.0SerXaa: 0.0 ± 0.0
Thr
4.507ThrAla: 4.507 ± 0.759
0.361ThrCys: 0.361 ± 0.242
1.082ThrAsp: 1.082 ± 0.417
2.344ThrGlu: 2.344 ± 0.715
1.082ThrPhe: 1.082 ± 0.413
4.507ThrGly: 4.507 ± 0.966
1.983ThrHis: 1.983 ± 0.69
5.048ThrIle: 5.048 ± 0.893
2.884ThrLys: 2.884 ± 0.753
5.949ThrLeu: 5.949 ± 1.262
0.721ThrMet: 0.721 ± 0.309
1.622ThrAsn: 1.622 ± 0.499
3.425ThrPro: 3.425 ± 0.81
1.082ThrGln: 1.082 ± 0.454
1.983ThrArg: 1.983 ± 0.592
4.687ThrSer: 4.687 ± 0.708
5.408ThrThr: 5.408 ± 1.53
5.228ThrVal: 5.228 ± 1.055
0.901ThrTrp: 0.901 ± 0.279
4.146ThrTyr: 4.146 ± 1.281
0.0ThrXaa: 0.0 ± 0.0
Val
7.932ValAla: 7.932 ± 0.988
1.082ValCys: 1.082 ± 0.526
2.344ValAsp: 2.344 ± 0.647
2.524ValGlu: 2.524 ± 0.768
2.524ValPhe: 2.524 ± 0.657
3.786ValGly: 3.786 ± 0.847
1.803ValHis: 1.803 ± 0.607
7.572ValIle: 7.572 ± 1.095
5.769ValLys: 5.769 ± 1.219
10.096ValLeu: 10.096 ± 1.451
1.442ValMet: 1.442 ± 0.436
3.606ValAsn: 3.606 ± 0.931
4.327ValPro: 4.327 ± 0.782
1.622ValGln: 1.622 ± 0.567
3.065ValArg: 3.065 ± 0.643
6.129ValSer: 6.129 ± 1.006
5.949ValThr: 5.949 ± 1.177
9.555ValVal: 9.555 ± 1.697
0.541ValTrp: 0.541 ± 0.267
6.851ValTyr: 6.851 ± 1.187
0.0ValXaa: 0.0 ± 0.0
Trp
1.622TrpAla: 1.622 ± 0.453
0.361TrpCys: 0.361 ± 0.272
0.721TrpAsp: 0.721 ± 0.304
0.541TrpGlu: 0.541 ± 0.303
0.901TrpPhe: 0.901 ± 0.365
0.901TrpGly: 0.901 ± 0.401
0.18TrpHis: 0.18 ± 0.159
1.442TrpIle: 1.442 ± 0.551
0.541TrpLys: 0.541 ± 0.336
1.983TrpLeu: 1.983 ± 0.703
0.541TrpMet: 0.541 ± 0.254
0.18TrpAsn: 0.18 ± 0.161
0.18TrpPro: 0.18 ± 0.175
0.18TrpGln: 0.18 ± 0.168
0.541TrpArg: 0.541 ± 0.327
0.721TrpSer: 0.721 ± 0.339
1.082TrpThr: 1.082 ± 0.443
0.901TrpVal: 0.901 ± 0.361
0.0TrpTrp: 0.0 ± 0.0
0.901TrpTyr: 0.901 ± 0.345
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.687TyrAla: 4.687 ± 0.893
0.901TyrCys: 0.901 ± 0.439
2.163TyrAsp: 2.163 ± 0.727
2.344TyrGlu: 2.344 ± 0.59
2.524TyrPhe: 2.524 ± 0.58
3.966TyrGly: 3.966 ± 0.803
0.721TyrHis: 0.721 ± 0.357
4.327TyrIle: 4.327 ± 0.737
3.425TyrLys: 3.425 ± 0.714
5.048TyrLeu: 5.048 ± 1.017
0.721TyrMet: 0.721 ± 0.325
3.245TyrAsn: 3.245 ± 0.974
2.704TyrPro: 2.704 ± 0.651
1.983TyrGln: 1.983 ± 0.685
2.163TyrArg: 2.163 ± 0.524
2.704TyrSer: 2.704 ± 0.589
3.425TyrThr: 3.425 ± 0.839
5.228TyrVal: 5.228 ± 0.889
0.541TyrTrp: 0.541 ± 0.233
4.867TyrTyr: 4.867 ± 1.251
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 32 proteins (5548 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski