Amino acid dipepetide frequency for Lactobacillus phage phiLdb

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.106AlaAla: 2.106 ± 1.086
0.67AlaCys: 0.67 ± 0.238
4.787AlaAsp: 4.787 ± 0.647
4.213AlaGlu: 4.213 ± 0.51
3.16AlaPhe: 3.16 ± 0.485
5.17AlaGly: 5.17 ± 1.326
0.766AlaHis: 0.766 ± 0.285
4.883AlaIle: 4.883 ± 0.778
6.032AlaLys: 6.032 ± 0.826
4.883AlaLeu: 4.883 ± 0.879
2.011AlaMet: 2.011 ± 0.554
4.5AlaAsn: 4.5 ± 0.819
1.149AlaPro: 1.149 ± 0.311
2.394AlaGln: 2.394 ± 0.922
2.681AlaArg: 2.681 ± 0.726
4.787AlaSer: 4.787 ± 1.063
2.872AlaThr: 2.872 ± 0.691
4.787AlaVal: 4.787 ± 0.807
1.053AlaTrp: 1.053 ± 0.27
3.351AlaTyr: 3.351 ± 0.739
0.0AlaXaa: 0.0 ± 0.0
Cys
0.287CysAla: 0.287 ± 0.173
0.287CysCys: 0.287 ± 0.173
0.191CysAsp: 0.191 ± 0.133
1.245CysGlu: 1.245 ± 0.396
0.0CysPhe: 0.0 ± 0.0
0.957CysGly: 0.957 ± 0.274
0.0CysHis: 0.0 ± 0.0
0.766CysIle: 0.766 ± 0.322
1.149CysLys: 1.149 ± 0.351
0.766CysLeu: 0.766 ± 0.342
0.287CysMet: 0.287 ± 0.146
0.479CysAsn: 0.479 ± 0.217
0.191CysPro: 0.191 ± 0.126
0.479CysGln: 0.479 ± 0.212
0.67CysArg: 0.67 ± 0.243
0.479CysSer: 0.479 ± 0.279
0.67CysThr: 0.67 ± 0.253
0.479CysVal: 0.479 ± 0.189
0.191CysTrp: 0.191 ± 0.15
0.287CysTyr: 0.287 ± 0.183
0.0CysXaa: 0.0 ± 0.0
Asp
2.777AspAla: 2.777 ± 0.43
0.862AspCys: 0.862 ± 0.309
5.649AspAsp: 5.649 ± 1.204
6.224AspGlu: 6.224 ± 0.969
2.681AspPhe: 2.681 ± 0.464
5.362AspGly: 5.362 ± 0.804
0.479AspHis: 0.479 ± 0.2
4.5AspIle: 4.5 ± 0.881
4.596AspLys: 4.596 ± 0.53
6.511AspLeu: 6.511 ± 0.946
2.968AspMet: 2.968 ± 0.56
4.979AspAsn: 4.979 ± 0.872
1.053AspPro: 1.053 ± 0.333
2.394AspGln: 2.394 ± 0.328
1.723AspArg: 1.723 ± 0.432
4.5AspSer: 4.5 ± 0.563
3.734AspThr: 3.734 ± 0.677
4.404AspVal: 4.404 ± 0.474
0.67AspTrp: 0.67 ± 0.262
3.255AspTyr: 3.255 ± 0.581
0.0AspXaa: 0.0 ± 0.0
Glu
5.17GluAla: 5.17 ± 0.642
0.479GluCys: 0.479 ± 0.209
6.128GluAsp: 6.128 ± 0.662
6.415GluGlu: 6.415 ± 0.975
2.298GluPhe: 2.298 ± 0.38
2.968GluGly: 2.968 ± 0.512
0.862GluHis: 0.862 ± 0.296
6.607GluIle: 6.607 ± 1.099
7.085GluLys: 7.085 ± 1.069
5.649GluLeu: 5.649 ± 0.846
3.064GluMet: 3.064 ± 0.623
4.213GluAsn: 4.213 ± 0.847
1.436GluPro: 1.436 ± 0.319
2.202GluGln: 2.202 ± 0.465
2.681GluArg: 2.681 ± 0.632
2.394GluSer: 2.394 ± 0.424
3.926GluThr: 3.926 ± 0.742
4.979GluVal: 4.979 ± 0.728
0.766GluTrp: 0.766 ± 0.228
3.064GluTyr: 3.064 ± 0.54
0.0GluXaa: 0.0 ± 0.0
Phe
2.106PheAla: 2.106 ± 0.479
0.287PheCys: 0.287 ± 0.165
2.298PheAsp: 2.298 ± 0.405
2.777PheGlu: 2.777 ± 0.485
1.245PhePhe: 1.245 ± 0.417
3.255PheGly: 3.255 ± 0.642
0.574PheHis: 0.574 ± 0.211
3.255PheIle: 3.255 ± 0.537
3.351PheLys: 3.351 ± 0.537
3.16PheLeu: 3.16 ± 0.689
0.766PheMet: 0.766 ± 0.291
3.064PheAsn: 3.064 ± 0.547
0.67PhePro: 0.67 ± 0.258
1.053PheGln: 1.053 ± 0.325
1.532PheArg: 1.532 ± 0.388
2.298PheSer: 2.298 ± 0.482
3.064PheThr: 3.064 ± 0.586
2.202PheVal: 2.202 ± 0.39
0.191PheTrp: 0.191 ± 0.127
0.766PheTyr: 0.766 ± 0.291
0.0PheXaa: 0.0 ± 0.0
Gly
4.117GlyAla: 4.117 ± 0.951
0.287GlyCys: 0.287 ± 0.174
4.692GlyAsp: 4.692 ± 0.804
2.681GlyGlu: 2.681 ± 0.509
3.447GlyPhe: 3.447 ± 0.588
3.83GlyGly: 3.83 ± 0.87
1.053GlyHis: 1.053 ± 0.33
5.553GlyIle: 5.553 ± 0.946
5.745GlyLys: 5.745 ± 0.762
5.649GlyLeu: 5.649 ± 0.794
1.34GlyMet: 1.34 ± 0.502
4.404GlyAsn: 4.404 ± 0.827
0.0GlyPro: 0.0 ± 0.0
1.532GlyGln: 1.532 ± 0.354
2.777GlyArg: 2.777 ± 0.548
4.404GlySer: 4.404 ± 0.932
3.543GlyThr: 3.543 ± 0.532
4.596GlyVal: 4.596 ± 0.65
0.862GlyTrp: 0.862 ± 0.237
3.638GlyTyr: 3.638 ± 0.647
0.0GlyXaa: 0.0 ± 0.0
His
0.766HisAla: 0.766 ± 0.305
0.096HisCys: 0.096 ± 0.088
0.957HisAsp: 0.957 ± 0.288
1.149HisGlu: 1.149 ± 0.391
0.287HisPhe: 0.287 ± 0.165
0.862HisGly: 0.862 ± 0.314
0.479HisHis: 0.479 ± 0.265
0.957HisIle: 0.957 ± 0.31
1.149HisLys: 1.149 ± 0.344
1.34HisLeu: 1.34 ± 0.495
0.191HisMet: 0.191 ± 0.113
0.383HisAsn: 0.383 ± 0.175
0.191HisPro: 0.191 ± 0.126
0.479HisGln: 0.479 ± 0.199
0.479HisArg: 0.479 ± 0.218
0.862HisSer: 0.862 ± 0.264
0.67HisThr: 0.67 ± 0.254
1.34HisVal: 1.34 ± 0.356
0.096HisTrp: 0.096 ± 0.109
1.053HisTyr: 1.053 ± 0.293
0.0HisXaa: 0.0 ± 0.0
Ile
4.692IleAla: 4.692 ± 0.576
0.479IleCys: 0.479 ± 0.219
5.458IleAsp: 5.458 ± 0.773
5.745IleGlu: 5.745 ± 0.756
1.34IlePhe: 1.34 ± 0.368
3.543IleGly: 3.543 ± 0.714
0.766IleHis: 0.766 ± 0.253
3.734IleIle: 3.734 ± 0.662
7.373IleLys: 7.373 ± 1.041
4.787IleLeu: 4.787 ± 1.004
2.011IleMet: 2.011 ± 0.43
5.553IleAsn: 5.553 ± 0.558
2.968IlePro: 2.968 ± 0.603
2.011IleGln: 2.011 ± 0.412
2.394IleArg: 2.394 ± 0.457
4.596IleSer: 4.596 ± 0.73
5.075IleThr: 5.075 ± 0.725
3.926IleVal: 3.926 ± 0.691
0.862IleTrp: 0.862 ± 0.305
3.16IleTyr: 3.16 ± 0.419
0.0IleXaa: 0.0 ± 0.0
Lys
6.511LysAla: 6.511 ± 0.873
0.287LysCys: 0.287 ± 0.171
4.213LysAsp: 4.213 ± 0.606
8.139LysGlu: 8.139 ± 1.02
2.777LysPhe: 2.777 ± 0.606
5.841LysGly: 5.841 ± 0.697
1.245LysHis: 1.245 ± 0.478
6.415LysIle: 6.415 ± 0.778
6.894LysLys: 6.894 ± 1.1
7.277LysLeu: 7.277 ± 0.772
2.202LysMet: 2.202 ± 0.491
6.607LysAsn: 6.607 ± 0.939
1.628LysPro: 1.628 ± 0.314
3.255LysGln: 3.255 ± 0.545
4.021LysArg: 4.021 ± 0.638
4.404LysSer: 4.404 ± 0.659
5.553LysThr: 5.553 ± 0.615
6.032LysVal: 6.032 ± 0.968
0.574LysTrp: 0.574 ± 0.255
3.351LysTyr: 3.351 ± 0.536
0.0LysXaa: 0.0 ± 0.0
Leu
4.883LeuAla: 4.883 ± 0.838
0.957LeuCys: 0.957 ± 0.324
6.224LeuAsp: 6.224 ± 1.018
5.17LeuGlu: 5.17 ± 0.755
2.777LeuPhe: 2.777 ± 0.599
4.979LeuGly: 4.979 ± 0.974
1.436LeuHis: 1.436 ± 0.364
5.553LeuIle: 5.553 ± 0.731
6.99LeuLys: 6.99 ± 0.943
6.319LeuLeu: 6.319 ± 1.114
1.628LeuMet: 1.628 ± 0.352
4.309LeuAsn: 4.309 ± 0.577
2.681LeuPro: 2.681 ± 0.597
1.723LeuGln: 1.723 ± 0.319
3.734LeuArg: 3.734 ± 0.567
4.5LeuSer: 4.5 ± 0.773
4.692LeuThr: 4.692 ± 0.62
4.596LeuVal: 4.596 ± 0.676
0.479LeuTrp: 0.479 ± 0.203
2.968LeuTyr: 2.968 ± 0.656
0.0LeuXaa: 0.0 ± 0.0
Met
2.968MetAla: 2.968 ± 0.574
0.287MetCys: 0.287 ± 0.179
1.723MetAsp: 1.723 ± 0.497
1.915MetGlu: 1.915 ± 0.451
0.479MetPhe: 0.479 ± 0.228
1.053MetGly: 1.053 ± 0.259
0.191MetHis: 0.191 ± 0.137
2.011MetIle: 2.011 ± 0.418
3.16MetLys: 3.16 ± 0.696
1.34MetLeu: 1.34 ± 0.405
0.766MetMet: 0.766 ± 0.239
1.532MetAsn: 1.532 ± 0.338
0.479MetPro: 0.479 ± 0.22
0.766MetGln: 0.766 ± 0.247
1.628MetArg: 1.628 ± 0.437
1.436MetSer: 1.436 ± 0.419
2.106MetThr: 2.106 ± 0.442
1.915MetVal: 1.915 ± 0.447
0.287MetTrp: 0.287 ± 0.136
0.096MetTyr: 0.096 ± 0.106
0.0MetXaa: 0.0 ± 0.0
Asn
5.266AsnAla: 5.266 ± 1.006
0.766AsnCys: 0.766 ± 0.251
3.351AsnAsp: 3.351 ± 0.721
4.883AsnGlu: 4.883 ± 0.748
2.872AsnPhe: 2.872 ± 0.603
7.468AsnGly: 7.468 ± 0.856
1.053AsnHis: 1.053 ± 0.419
3.734AsnIle: 3.734 ± 0.571
4.979AsnLys: 4.979 ± 0.799
4.309AsnLeu: 4.309 ± 0.752
1.34AsnMet: 1.34 ± 0.334
4.213AsnAsn: 4.213 ± 0.587
1.915AsnPro: 1.915 ± 0.517
2.106AsnGln: 2.106 ± 0.432
2.298AsnArg: 2.298 ± 0.46
5.075AsnSer: 5.075 ± 0.728
2.681AsnThr: 2.681 ± 0.606
3.926AsnVal: 3.926 ± 0.499
0.766AsnTrp: 0.766 ± 0.284
2.968AsnTyr: 2.968 ± 0.494
0.0AsnXaa: 0.0 ± 0.0
Pro
1.34ProAla: 1.34 ± 0.399
0.191ProCys: 0.191 ± 0.126
1.245ProAsp: 1.245 ± 0.406
0.862ProGlu: 0.862 ± 0.314
0.957ProPhe: 0.957 ± 0.273
1.149ProGly: 1.149 ± 0.494
0.191ProHis: 0.191 ± 0.159
1.34ProIle: 1.34 ± 0.336
2.585ProLys: 2.585 ± 0.501
1.245ProLeu: 1.245 ± 0.348
0.287ProMet: 0.287 ± 0.167
1.628ProAsn: 1.628 ± 0.405
0.479ProPro: 0.479 ± 0.162
0.67ProGln: 0.67 ± 0.265
1.053ProArg: 1.053 ± 0.257
2.011ProSer: 2.011 ± 0.404
1.628ProThr: 1.628 ± 0.303
2.394ProVal: 2.394 ± 0.504
0.479ProTrp: 0.479 ± 0.214
1.245ProTyr: 1.245 ± 0.389
0.0ProXaa: 0.0 ± 0.0
Gln
2.872GlnAla: 2.872 ± 0.653
0.383GlnCys: 0.383 ± 0.254
2.202GlnAsp: 2.202 ± 0.352
1.436GlnGlu: 1.436 ± 0.347
1.053GlnPhe: 1.053 ± 0.24
1.532GlnGly: 1.532 ± 0.251
0.67GlnHis: 0.67 ± 0.225
2.968GlnIle: 2.968 ± 0.425
2.202GlnLys: 2.202 ± 0.602
3.255GlnLeu: 3.255 ± 0.87
1.053GlnMet: 1.053 ± 0.35
1.819GlnAsn: 1.819 ± 0.495
1.053GlnPro: 1.053 ± 0.328
1.245GlnGln: 1.245 ± 0.383
1.245GlnArg: 1.245 ± 0.306
1.915GlnSer: 1.915 ± 0.52
1.723GlnThr: 1.723 ± 0.459
2.394GlnVal: 2.394 ± 0.413
0.096GlnTrp: 0.096 ± 0.088
1.819GlnTyr: 1.819 ± 0.356
0.0GlnXaa: 0.0 ± 0.0
Arg
3.638ArgAla: 3.638 ± 0.48
0.766ArgCys: 0.766 ± 0.289
2.872ArgAsp: 2.872 ± 0.475
2.968ArgGlu: 2.968 ± 0.583
2.106ArgPhe: 2.106 ± 0.374
1.532ArgGly: 1.532 ± 0.389
0.383ArgHis: 0.383 ± 0.21
2.872ArgIle: 2.872 ± 0.447
4.213ArgLys: 4.213 ± 0.533
3.255ArgLeu: 3.255 ± 0.486
1.245ArgMet: 1.245 ± 0.286
2.489ArgAsn: 2.489 ± 0.593
0.67ArgPro: 0.67 ± 0.253
1.436ArgGln: 1.436 ± 0.304
1.723ArgArg: 1.723 ± 0.397
1.819ArgSer: 1.819 ± 0.408
2.202ArgThr: 2.202 ± 0.422
1.723ArgVal: 1.723 ± 0.31
0.862ArgTrp: 0.862 ± 0.278
2.106ArgTyr: 2.106 ± 0.39
0.0ArgXaa: 0.0 ± 0.0
Ser
4.404SerAla: 4.404 ± 0.736
0.766SerCys: 0.766 ± 0.259
3.734SerAsp: 3.734 ± 0.524
4.213SerGlu: 4.213 ± 0.551
3.543SerPhe: 3.543 ± 0.652
4.021SerGly: 4.021 ± 0.639
0.862SerHis: 0.862 ± 0.25
2.872SerIle: 2.872 ± 0.443
5.553SerLys: 5.553 ± 0.617
3.734SerLeu: 3.734 ± 0.743
1.628SerMet: 1.628 ± 0.498
4.787SerAsn: 4.787 ± 0.733
1.819SerPro: 1.819 ± 0.368
2.394SerGln: 2.394 ± 0.605
2.202SerArg: 2.202 ± 0.642
4.021SerSer: 4.021 ± 0.66
3.351SerThr: 3.351 ± 0.692
4.979SerVal: 4.979 ± 0.576
0.67SerTrp: 0.67 ± 0.26
2.777SerTyr: 2.777 ± 0.595
0.0SerXaa: 0.0 ± 0.0
Thr
4.117ThrAla: 4.117 ± 0.631
0.383ThrCys: 0.383 ± 0.188
3.926ThrAsp: 3.926 ± 0.551
4.213ThrGlu: 4.213 ± 0.621
2.585ThrPhe: 2.585 ± 0.459
3.734ThrGly: 3.734 ± 0.629
0.766ThrHis: 0.766 ± 0.267
4.117ThrIle: 4.117 ± 0.671
5.362ThrLys: 5.362 ± 0.798
4.213ThrLeu: 4.213 ± 0.795
0.287ThrMet: 0.287 ± 0.162
2.968ThrAsn: 2.968 ± 0.917
1.532ThrPro: 1.532 ± 0.324
2.011ThrGln: 2.011 ± 0.496
2.298ThrArg: 2.298 ± 0.541
3.638ThrSer: 3.638 ± 0.697
3.351ThrThr: 3.351 ± 0.628
5.17ThrVal: 5.17 ± 0.843
0.766ThrTrp: 0.766 ± 0.233
2.968ThrTyr: 2.968 ± 0.549
0.0ThrXaa: 0.0 ± 0.0
Val
5.841ValAla: 5.841 ± 0.859
0.862ValCys: 0.862 ± 0.341
4.787ValAsp: 4.787 ± 0.566
4.883ValGlu: 4.883 ± 0.738
2.011ValPhe: 2.011 ± 0.515
3.926ValGly: 3.926 ± 0.656
1.245ValHis: 1.245 ± 0.294
4.883ValIle: 4.883 ± 0.556
5.458ValLys: 5.458 ± 0.814
4.596ValLeu: 4.596 ± 0.632
1.532ValMet: 1.532 ± 0.354
4.692ValAsn: 4.692 ± 0.732
1.723ValPro: 1.723 ± 0.535
2.106ValGln: 2.106 ± 0.429
2.394ValArg: 2.394 ± 0.419
5.17ValSer: 5.17 ± 0.578
4.117ValThr: 4.117 ± 0.623
4.021ValVal: 4.021 ± 0.62
0.479ValTrp: 0.479 ± 0.227
3.064ValTyr: 3.064 ± 0.48
0.0ValXaa: 0.0 ± 0.0
Trp
0.766TrpAla: 0.766 ± 0.305
0.191TrpCys: 0.191 ± 0.131
0.479TrpAsp: 0.479 ± 0.238
0.766TrpGlu: 0.766 ± 0.313
0.862TrpPhe: 0.862 ± 0.335
0.67TrpGly: 0.67 ± 0.233
0.383TrpHis: 0.383 ± 0.189
0.67TrpIle: 0.67 ± 0.229
0.766TrpLys: 0.766 ± 0.246
1.34TrpLeu: 1.34 ± 0.325
0.0TrpMet: 0.0 ± 0.0
0.766TrpAsn: 0.766 ± 0.409
0.0TrpPro: 0.0 ± 0.0
0.287TrpGln: 0.287 ± 0.152
0.479TrpArg: 0.479 ± 0.228
0.479TrpSer: 0.479 ± 0.232
0.67TrpThr: 0.67 ± 0.211
0.574TrpVal: 0.574 ± 0.255
0.0TrpTrp: 0.0 ± 0.0
0.383TrpTyr: 0.383 ± 0.19
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.628TyrAla: 1.628 ± 0.441
0.574TyrCys: 0.574 ± 0.301
4.404TyrAsp: 4.404 ± 0.517
2.489TyrGlu: 2.489 ± 0.549
1.436TyrPhe: 1.436 ± 0.367
2.298TyrGly: 2.298 ± 0.488
0.383TyrHis: 0.383 ± 0.173
2.777TyrIle: 2.777 ± 0.511
2.585TyrLys: 2.585 ± 0.581
2.968TyrLeu: 2.968 ± 0.615
1.436TyrMet: 1.436 ± 0.339
2.585TyrAsn: 2.585 ± 0.424
1.34TyrPro: 1.34 ± 0.332
2.394TyrGln: 2.394 ± 0.507
2.872TyrArg: 2.872 ± 0.606
3.638TyrSer: 3.638 ± 0.671
2.777TyrThr: 2.777 ± 0.493
3.351TyrVal: 3.351 ± 0.585
0.383TyrTrp: 0.383 ± 0.195
1.723TyrTyr: 1.723 ± 0.457
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (10445 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski