Streptomyces alboflavus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9243 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z1WBM9|A0A1Z1WBM9_9ACTN Uncharacterized protein OS=Streptomyces alboflavus OX=67267 GN=SMD44_03284 PE=4 SV=1
MM1 pKa = 7.92TDD3 pKa = 3.1GQRR6 pKa = 11.84NGAGGWQPHH15 pKa = 5.66PQGEE19 pKa = 4.29YY20 pKa = 10.71DD21 pKa = 3.58ADD23 pKa = 3.31ATAFVQLPEE32 pKa = 4.24GAADD36 pKa = 3.71AVSTPLAAPGHH47 pKa = 6.44GYY49 pKa = 9.98VPPQITVTPTAPDD62 pKa = 3.39ATDD65 pKa = 3.66PAATGAWVLPPEE77 pKa = 4.51VTGVAHH83 pKa = 6.76TGGATQAVSWPDD95 pKa = 3.34PGTQLGTHH103 pKa = 5.83QEE105 pKa = 4.13QQFPAEE111 pKa = 4.08PQVPDD116 pKa = 4.13AYY118 pKa = 10.68DD119 pKa = 3.48PRR121 pKa = 11.84ATGQWTFPDD130 pKa = 3.67AVTPPEE136 pKa = 4.06QNGGQGGVQNGHH148 pKa = 6.71APAAHH153 pKa = 7.08DD154 pKa = 3.96VTGQWSIPVADD165 pKa = 4.18GDD167 pKa = 4.0LPEE170 pKa = 4.58EE171 pKa = 4.29SGEE174 pKa = 4.06YY175 pKa = 9.79SASSLAAQWGGAPPPATLPGGAPAPWATSWPTQAPEE211 pKa = 4.07APQVPQAPQAPQVPQAPEE229 pKa = 4.03LPHH232 pKa = 8.43APEE235 pKa = 5.03APQASPYY242 pKa = 9.66PAAQAFVEE250 pKa = 4.31QAPLGPPAPAQEE262 pKa = 4.41SAPVPPARR270 pKa = 11.84AAEE273 pKa = 4.02EE274 pKa = 4.54TPFEE278 pKa = 4.09RR279 pKa = 11.84TAKK282 pKa = 10.5LFVVKK287 pKa = 9.74QRR289 pKa = 11.84PDD291 pKa = 3.02GSLAPAGSPGDD302 pKa = 3.68VTDD305 pKa = 5.36APDD308 pKa = 3.65APGAPDD314 pKa = 3.71VPGMPDD320 pKa = 2.97ASGEE324 pKa = 4.46TVNPVDD330 pKa = 3.66PAALEE335 pKa = 4.55GDD337 pKa = 4.85FGPQSAASTHH347 pKa = 6.53LAPAADD353 pKa = 3.9PHH355 pKa = 7.3YY356 pKa = 10.44GADD359 pKa = 3.44PAYY362 pKa = 10.57DD363 pKa = 3.44EE364 pKa = 4.32NAYY367 pKa = 10.53DD368 pKa = 3.58EE369 pKa = 4.41SAPYY373 pKa = 10.2EE374 pKa = 4.27EE375 pKa = 4.32NTPYY379 pKa = 11.39DD380 pKa = 3.86MPPVQSADD388 pKa = 3.33LAQSVGLAPAPGFPHH403 pKa = 6.48TVDD406 pKa = 3.05AVYY409 pKa = 9.16PADD412 pKa = 4.15PAHH415 pKa = 6.35ATDD418 pKa = 4.58PSYY421 pKa = 11.72DD422 pKa = 4.08PDD424 pKa = 4.36PALAPPPAHH433 pKa = 6.61PAEE436 pKa = 4.54PAADD440 pKa = 4.0PAHH443 pKa = 6.77PAAVAPAVPPHH454 pKa = 6.33EE455 pKa = 5.33ADD457 pKa = 3.74PAHH460 pKa = 6.37VPAPAQAADD469 pKa = 4.07PAPAGNPAPAPEE481 pKa = 3.93QAHH484 pKa = 5.81AAEE487 pKa = 5.93AIPAADD493 pKa = 4.24PAPAPVPEE501 pKa = 5.33PDD503 pKa = 3.55PAPDD507 pKa = 4.38AGHH510 pKa = 6.36TPDD513 pKa = 4.36PEE515 pKa = 4.07EE516 pKa = 4.16PAAADD521 pKa = 4.08DD522 pKa = 4.9APEE525 pKa = 5.26ADD527 pKa = 5.1DD528 pKa = 4.97PAEE531 pKa = 4.46ADD533 pKa = 3.08QDD535 pKa = 4.19PEE537 pKa = 4.25VEE539 pKa = 4.4HH540 pKa = 7.81APEE543 pKa = 4.8ADD545 pKa = 3.52HH546 pKa = 7.52ASEE549 pKa = 5.4ADD551 pKa = 3.67DD552 pKa = 5.57AEE554 pKa = 5.84ADD556 pKa = 3.94LAADD560 pKa = 4.04SAPVEE565 pKa = 4.12ADD567 pKa = 3.42APVADD572 pKa = 4.97APDD575 pKa = 3.88NAPEE579 pKa = 4.63PDD581 pKa = 3.64APAHH585 pKa = 6.61DD586 pKa = 4.05EE587 pKa = 4.45HH588 pKa = 7.64PLVSYY593 pKa = 8.37VLRR596 pKa = 11.84VNGADD601 pKa = 4.66RR602 pKa = 11.84PVTDD606 pKa = 3.02AWIGEE611 pKa = 4.15SLLYY615 pKa = 10.18VLRR618 pKa = 11.84EE619 pKa = 3.94RR620 pKa = 11.84LGLAGAKK627 pKa = 9.72DD628 pKa = 3.75GCSQGEE634 pKa = 4.12CGACNVQVDD643 pKa = 3.94GRR645 pKa = 11.84LVASCLVPAATAAGSEE661 pKa = 4.08VRR663 pKa = 11.84TVEE666 pKa = 4.04GLAADD671 pKa = 4.46GRR673 pKa = 11.84PSDD676 pKa = 3.9VQRR679 pKa = 11.84ALANCGAVQCGFCVPGMAMTLHH701 pKa = 7.21DD702 pKa = 5.03LLEE705 pKa = 4.8GNPAPSDD712 pKa = 3.69LEE714 pKa = 4.15TRR716 pKa = 11.84RR717 pKa = 11.84ALCGNLCRR725 pKa = 11.84CSGYY729 pKa = 10.39RR730 pKa = 11.84GVLDD734 pKa = 3.66AVRR737 pKa = 11.84DD738 pKa = 4.07VVAEE742 pKa = 4.26RR743 pKa = 11.84EE744 pKa = 4.49AKK746 pKa = 10.02GAAAEE751 pKa = 4.1QRR753 pKa = 11.84DD754 pKa = 4.18DD755 pKa = 4.86ADD757 pKa = 3.46TGTPRR762 pKa = 11.84VPHH765 pKa = 5.37QSGPYY770 pKa = 8.94GQDD773 pKa = 2.85GAQAA777 pKa = 3.71

Molecular weight: 78.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z1WET9|A0A1Z1WET9_9ACTN RNA polymerase sigma factor SigM OS=Streptomyces alboflavus OX=67267 GN=SMD44_04317 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.74GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9243

0

9243

2826290

26

4855

305.8

32.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.962 ± 0.039

0.832 ± 0.008

6.135 ± 0.023

5.545 ± 0.024

2.652 ± 0.014

9.657 ± 0.032

2.331 ± 0.013

2.895 ± 0.017

2.296 ± 0.024

10.106 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.702 ± 0.011

1.638 ± 0.012

6.265 ± 0.027

2.682 ± 0.017

8.227 ± 0.029

5.092 ± 0.02

6.085 ± 0.023

8.374 ± 0.027

1.524 ± 0.011

1.999 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski