Amino acid dipepetide frequency for Pseudomonas phage vB_PaM_EPA1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.949AlaAla: 6.949 ± 0.599
1.36AlaCys: 1.36 ± 0.219
4.412AlaAsp: 4.412 ± 0.47
5.736AlaGlu: 5.736 ± 0.421
3.052AlaPhe: 3.052 ± 0.324
5.258AlaGly: 5.258 ± 0.486
1.875AlaHis: 1.875 ± 0.258
5.111AlaIle: 5.111 ± 0.408
4.964AlaLys: 4.964 ± 0.474
6.581AlaLeu: 6.581 ± 0.557
3.015AlaMet: 3.015 ± 0.304
3.346AlaAsn: 3.346 ± 0.469
2.243AlaPro: 2.243 ± 0.303
3.236AlaGln: 3.236 ± 0.429
4.964AlaArg: 4.964 ± 0.371
4.118AlaSer: 4.118 ± 0.387
5.147AlaThr: 5.147 ± 0.499
5.405AlaVal: 5.405 ± 0.445
1.434AlaTrp: 1.434 ± 0.238
2.905AlaTyr: 2.905 ± 0.305
0.0AlaXaa: 0.0 ± 0.0
Cys
0.919CysAla: 0.919 ± 0.219
0.257CysCys: 0.257 ± 0.089
0.846CysAsp: 0.846 ± 0.174
1.213CysGlu: 1.213 ± 0.214
0.441CysPhe: 0.441 ± 0.132
1.103CysGly: 1.103 ± 0.223
0.257CysHis: 0.257 ± 0.091
0.882CysIle: 0.882 ± 0.153
0.919CysLys: 0.919 ± 0.185
0.662CysLeu: 0.662 ± 0.166
0.588CysMet: 0.588 ± 0.14
0.882CysAsn: 0.882 ± 0.185
0.515CysPro: 0.515 ± 0.15
0.552CysGln: 0.552 ± 0.13
0.882CysArg: 0.882 ± 0.17
0.809CysSer: 0.809 ± 0.175
0.368CysThr: 0.368 ± 0.114
0.662CysVal: 0.662 ± 0.138
0.294CysTrp: 0.294 ± 0.1
0.662CysTyr: 0.662 ± 0.159
0.0CysXaa: 0.0 ± 0.0
Asp
4.559AspAla: 4.559 ± 0.403
0.956AspCys: 0.956 ± 0.199
3.383AspAsp: 3.383 ± 0.345
3.787AspGlu: 3.787 ± 0.375
2.758AspPhe: 2.758 ± 0.29
4.339AspGly: 4.339 ± 0.37
1.507AspHis: 1.507 ± 0.249
4.008AspIle: 4.008 ± 0.436
3.493AspLys: 3.493 ± 0.378
5.258AspLeu: 5.258 ± 0.435
1.949AspMet: 1.949 ± 0.279
2.537AspAsn: 2.537 ± 0.314
2.941AspPro: 2.941 ± 0.366
1.838AspGln: 1.838 ± 0.281
3.53AspArg: 3.53 ± 0.381
3.088AspSer: 3.088 ± 0.337
3.236AspThr: 3.236 ± 0.311
3.934AspVal: 3.934 ± 0.406
1.397AspTrp: 1.397 ± 0.233
2.574AspTyr: 2.574 ± 0.335
0.0AspXaa: 0.0 ± 0.0
Glu
7.059GluAla: 7.059 ± 0.556
1.177GluCys: 1.177 ± 0.206
5.0GluAsp: 5.0 ± 0.394
7.537GluGlu: 7.537 ± 0.655
3.456GluPhe: 3.456 ± 0.303
5.442GluGly: 5.442 ± 0.478
1.36GluHis: 1.36 ± 0.248
3.861GluIle: 3.861 ± 0.34
3.787GluLys: 3.787 ± 0.405
6.839GluLeu: 6.839 ± 0.617
2.059GluMet: 2.059 ± 0.284
2.537GluAsn: 2.537 ± 0.255
1.875GluPro: 1.875 ± 0.253
2.721GluGln: 2.721 ± 0.286
4.302GluArg: 4.302 ± 0.377
3.566GluSer: 3.566 ± 0.315
3.456GluThr: 3.456 ± 0.365
5.809GluVal: 5.809 ± 0.494
1.691GluTrp: 1.691 ± 0.208
3.64GluTyr: 3.64 ± 0.372
0.0GluXaa: 0.0 ± 0.0
Phe
3.052PheAla: 3.052 ± 0.302
0.662PheCys: 0.662 ± 0.161
2.794PheAsp: 2.794 ± 0.423
3.309PheGlu: 3.309 ± 0.328
1.912PhePhe: 1.912 ± 0.27
2.794PheGly: 2.794 ± 0.337
0.735PheHis: 0.735 ± 0.165
1.728PheIle: 1.728 ± 0.255
3.309PheLys: 3.309 ± 0.367
2.941PheLeu: 2.941 ± 0.319
1.14PheMet: 1.14 ± 0.191
2.133PheAsn: 2.133 ± 0.313
1.434PhePro: 1.434 ± 0.209
1.471PheGln: 1.471 ± 0.21
1.985PheArg: 1.985 ± 0.239
2.537PheSer: 2.537 ± 0.311
2.794PheThr: 2.794 ± 0.358
2.905PheVal: 2.905 ± 0.304
0.882PheTrp: 0.882 ± 0.175
1.287PheTyr: 1.287 ± 0.22
0.0PheXaa: 0.0 ± 0.0
Gly
4.375GlyAla: 4.375 ± 0.475
1.213GlyCys: 1.213 ± 0.204
4.449GlyAsp: 4.449 ± 0.366
5.258GlyGlu: 5.258 ± 0.512
3.493GlyPhe: 3.493 ± 0.346
5.331GlyGly: 5.331 ± 0.551
1.177GlyHis: 1.177 ± 0.229
3.787GlyIle: 3.787 ± 0.412
4.339GlyLys: 4.339 ± 0.458
4.927GlyLeu: 4.927 ± 0.468
2.022GlyMet: 2.022 ± 0.291
2.978GlyAsn: 2.978 ± 0.367
1.471GlyPro: 1.471 ± 0.224
2.794GlyGln: 2.794 ± 0.331
3.75GlyArg: 3.75 ± 0.4
4.596GlySer: 4.596 ± 0.364
3.493GlyThr: 3.493 ± 0.395
5.442GlyVal: 5.442 ± 0.421
1.471GlyTrp: 1.471 ± 0.204
3.236GlyTyr: 3.236 ± 0.351
0.0GlyXaa: 0.0 ± 0.0
His
1.691HisAla: 1.691 ± 0.235
0.294HisCys: 0.294 ± 0.096
1.14HisAsp: 1.14 ± 0.222
0.662HisGlu: 0.662 ± 0.144
0.919HisPhe: 0.919 ± 0.213
1.507HisGly: 1.507 ± 0.25
0.368HisHis: 0.368 ± 0.142
1.213HisIle: 1.213 ± 0.198
1.25HisLys: 1.25 ± 0.244
1.618HisLeu: 1.618 ± 0.266
0.625HisMet: 0.625 ± 0.133
0.699HisAsn: 0.699 ± 0.171
0.846HisPro: 0.846 ± 0.166
0.478HisGln: 0.478 ± 0.128
1.103HisArg: 1.103 ± 0.221
1.066HisSer: 1.066 ± 0.174
1.434HisThr: 1.434 ± 0.239
1.14HisVal: 1.14 ± 0.195
0.441HisTrp: 0.441 ± 0.126
0.662HisTyr: 0.662 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
4.265IleAla: 4.265 ± 0.374
0.441IleCys: 0.441 ± 0.127
4.302IleAsp: 4.302 ± 0.379
3.971IleGlu: 3.971 ± 0.416
1.618IlePhe: 1.618 ± 0.23
3.419IleGly: 3.419 ± 0.397
1.471IleHis: 1.471 ± 0.241
2.463IleIle: 2.463 ± 0.283
4.008IleLys: 4.008 ± 0.419
4.375IleLeu: 4.375 ± 0.422
1.397IleMet: 1.397 ± 0.239
2.647IleAsn: 2.647 ± 0.389
2.537IlePro: 2.537 ± 0.297
1.912IleGln: 1.912 ± 0.252
3.861IleArg: 3.861 ± 0.424
3.419IleSer: 3.419 ± 0.368
2.721IleThr: 2.721 ± 0.3
3.824IleVal: 3.824 ± 0.417
0.735IleTrp: 0.735 ± 0.167
1.618IleTyr: 1.618 ± 0.229
0.0IleXaa: 0.0 ± 0.0
Lys
5.809LysAla: 5.809 ± 0.645
0.735LysCys: 0.735 ± 0.137
4.081LysAsp: 4.081 ± 0.368
5.662LysGlu: 5.662 ± 0.704
1.949LysPhe: 1.949 ± 0.287
4.265LysGly: 4.265 ± 0.357
1.103LysHis: 1.103 ± 0.189
3.309LysIle: 3.309 ± 0.329
3.75LysLys: 3.75 ± 0.309
5.074LysLeu: 5.074 ± 0.351
2.096LysMet: 2.096 ± 0.253
2.316LysAsn: 2.316 ± 0.305
1.912LysPro: 1.912 ± 0.262
2.169LysGln: 2.169 ± 0.288
3.566LysArg: 3.566 ± 0.448
3.603LysSer: 3.603 ± 0.368
3.125LysThr: 3.125 ± 0.314
4.339LysVal: 4.339 ± 0.372
1.066LysTrp: 1.066 ± 0.191
2.316LysTyr: 2.316 ± 0.301
0.0LysXaa: 0.0 ± 0.0
Leu
6.398LeuAla: 6.398 ± 0.515
0.882LeuCys: 0.882 ± 0.174
5.478LeuAsp: 5.478 ± 0.414
5.883LeuGlu: 5.883 ± 0.485
2.684LeuPhe: 2.684 ± 0.33
5.442LeuGly: 5.442 ± 0.493
1.655LeuHis: 1.655 ± 0.245
4.412LeuIle: 4.412 ± 0.443
5.442LeuLys: 5.442 ± 0.525
5.368LeuLeu: 5.368 ± 0.559
2.463LeuMet: 2.463 ± 0.25
3.088LeuAsn: 3.088 ± 0.445
3.714LeuPro: 3.714 ± 0.37
2.831LeuGln: 2.831 ± 0.261
4.449LeuArg: 4.449 ± 0.413
5.0LeuSer: 5.0 ± 0.491
4.669LeuThr: 4.669 ± 0.478
4.78LeuVal: 4.78 ± 0.423
1.324LeuTrp: 1.324 ± 0.199
3.015LeuTyr: 3.015 ± 0.357
0.0LeuXaa: 0.0 ± 0.0
Met
3.419MetAla: 3.419 ± 0.317
0.515MetCys: 0.515 ± 0.15
1.471MetAsp: 1.471 ± 0.265
2.941MetGlu: 2.941 ± 0.34
0.956MetPhe: 0.956 ± 0.181
1.838MetGly: 1.838 ± 0.274
0.221MetHis: 0.221 ± 0.088
1.324MetIle: 1.324 ± 0.208
2.206MetLys: 2.206 ± 0.273
1.838MetLeu: 1.838 ± 0.248
1.177MetMet: 1.177 ± 0.248
1.25MetAsn: 1.25 ± 0.235
1.103MetPro: 1.103 ± 0.198
1.581MetGln: 1.581 ± 0.238
1.655MetArg: 1.655 ± 0.23
2.096MetSer: 2.096 ± 0.226
2.28MetThr: 2.28 ± 0.259
1.544MetVal: 1.544 ± 0.275
0.404MetTrp: 0.404 ± 0.115
0.956MetTyr: 0.956 ± 0.185
0.0MetXaa: 0.0 ± 0.0
Asn
3.088AsnAla: 3.088 ± 0.439
0.257AsnCys: 0.257 ± 0.087
2.096AsnAsp: 2.096 ± 0.281
2.206AsnGlu: 2.206 ± 0.264
2.059AsnPhe: 2.059 ± 0.24
3.419AsnGly: 3.419 ± 0.374
0.882AsnHis: 0.882 ± 0.196
2.647AsnIle: 2.647 ± 0.281
2.684AsnLys: 2.684 ± 0.338
3.714AsnLeu: 3.714 ± 0.593
1.177AsnMet: 1.177 ± 0.177
1.691AsnAsn: 1.691 ± 0.319
1.949AsnPro: 1.949 ± 0.297
1.287AsnGln: 1.287 ± 0.261
1.765AsnArg: 1.765 ± 0.217
2.758AsnSer: 2.758 ± 0.363
2.169AsnThr: 2.169 ± 0.284
3.199AsnVal: 3.199 ± 0.401
0.735AsnTrp: 0.735 ± 0.179
1.324AsnTyr: 1.324 ± 0.186
0.0AsnXaa: 0.0 ± 0.0
Pro
3.052ProAla: 3.052 ± 0.316
0.515ProCys: 0.515 ± 0.136
2.353ProAsp: 2.353 ± 0.339
4.044ProGlu: 4.044 ± 0.324
1.434ProPhe: 1.434 ± 0.267
2.427ProGly: 2.427 ± 0.322
0.809ProHis: 0.809 ± 0.21
1.802ProIle: 1.802 ± 0.276
1.802ProLys: 1.802 ± 0.272
2.61ProLeu: 2.61 ± 0.318
0.882ProMet: 0.882 ± 0.168
1.14ProAsn: 1.14 ± 0.247
1.177ProPro: 1.177 ± 0.203
0.809ProGln: 0.809 ± 0.194
1.581ProArg: 1.581 ± 0.228
2.206ProSer: 2.206 ± 0.259
2.463ProThr: 2.463 ± 0.3
3.383ProVal: 3.383 ± 0.346
0.368ProTrp: 0.368 ± 0.118
1.287ProTyr: 1.287 ± 0.195
0.0ProXaa: 0.0 ± 0.0
Gln
3.346GlnAla: 3.346 ± 0.332
0.588GlnCys: 0.588 ± 0.143
1.912GlnAsp: 1.912 ± 0.248
2.5GlnGlu: 2.5 ± 0.321
1.838GlnPhe: 1.838 ± 0.277
1.949GlnGly: 1.949 ± 0.334
0.699GlnHis: 0.699 ± 0.163
2.206GlnIle: 2.206 ± 0.269
1.691GlnLys: 1.691 ± 0.274
3.015GlnLeu: 3.015 ± 0.311
1.544GlnMet: 1.544 ± 0.213
1.066GlnAsn: 1.066 ± 0.16
0.882GlnPro: 0.882 ± 0.216
0.956GlnGln: 0.956 ± 0.202
1.949GlnArg: 1.949 ± 0.249
1.949GlnSer: 1.949 ± 0.287
1.912GlnThr: 1.912 ± 0.266
2.684GlnVal: 2.684 ± 0.266
0.772GlnTrp: 0.772 ± 0.175
1.544GlnTyr: 1.544 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
4.302ArgAla: 4.302 ± 0.459
0.699ArgCys: 0.699 ± 0.148
3.236ArgAsp: 3.236 ± 0.31
4.228ArgGlu: 4.228 ± 0.379
2.39ArgPhe: 2.39 ± 0.291
3.897ArgGly: 3.897 ± 0.375
1.103ArgHis: 1.103 ± 0.192
2.905ArgIle: 2.905 ± 0.303
4.339ArgLys: 4.339 ± 0.404
4.89ArgLeu: 4.89 ± 0.469
1.618ArgMet: 1.618 ± 0.247
2.022ArgAsn: 2.022 ± 0.323
2.169ArgPro: 2.169 ± 0.271
2.427ArgGln: 2.427 ± 0.284
3.162ArgArg: 3.162 ± 0.401
2.941ArgSer: 2.941 ± 0.288
2.5ArgThr: 2.5 ± 0.29
4.191ArgVal: 4.191 ± 0.329
1.177ArgTrp: 1.177 ± 0.249
1.949ArgTyr: 1.949 ± 0.278
0.0ArgXaa: 0.0 ± 0.0
Ser
4.486SerAla: 4.486 ± 0.474
0.625SerCys: 0.625 ± 0.168
3.309SerAsp: 3.309 ± 0.354
4.302SerGlu: 4.302 ± 0.367
2.463SerPhe: 2.463 ± 0.298
4.044SerGly: 4.044 ± 0.447
0.772SerHis: 0.772 ± 0.18
2.868SerIle: 2.868 ± 0.376
3.383SerLys: 3.383 ± 0.331
4.706SerLeu: 4.706 ± 0.397
1.728SerMet: 1.728 ± 0.259
2.758SerAsn: 2.758 ± 0.343
2.316SerPro: 2.316 ± 0.306
1.655SerGln: 1.655 ± 0.285
3.714SerArg: 3.714 ± 0.419
3.015SerSer: 3.015 ± 0.38
2.978SerThr: 2.978 ± 0.283
5.111SerVal: 5.111 ± 0.408
0.772SerTrp: 0.772 ± 0.162
2.206SerTyr: 2.206 ± 0.249
0.0SerXaa: 0.0 ± 0.0
Thr
4.412ThrAla: 4.412 ± 0.523
0.772ThrCys: 0.772 ± 0.169
2.905ThrAsp: 2.905 ± 0.35
4.191ThrGlu: 4.191 ± 0.349
2.684ThrPhe: 2.684 ± 0.307
4.817ThrGly: 4.817 ± 0.464
1.029ThrHis: 1.029 ± 0.167
3.162ThrIle: 3.162 ± 0.267
2.574ThrLys: 2.574 ± 0.393
5.111ThrLeu: 5.111 ± 0.402
1.103ThrMet: 1.103 ± 0.195
2.206ThrAsn: 2.206 ± 0.273
2.133ThrPro: 2.133 ± 0.319
1.985ThrGln: 1.985 ± 0.291
2.684ThrArg: 2.684 ± 0.315
2.905ThrSer: 2.905 ± 0.339
2.794ThrThr: 2.794 ± 0.31
4.853ThrVal: 4.853 ± 0.554
0.846ThrTrp: 0.846 ± 0.184
2.133ThrTyr: 2.133 ± 0.3
0.0ThrXaa: 0.0 ± 0.0
Val
6.177ValAla: 6.177 ± 0.55
0.735ValCys: 0.735 ± 0.189
4.486ValAsp: 4.486 ± 0.393
5.846ValGlu: 5.846 ± 0.539
3.236ValPhe: 3.236 ± 0.32
4.522ValGly: 4.522 ± 0.421
1.25ValHis: 1.25 ± 0.198
3.971ValIle: 3.971 ± 0.433
4.633ValLys: 4.633 ± 0.391
5.515ValLeu: 5.515 ± 0.481
2.353ValMet: 2.353 ± 0.297
3.015ValAsn: 3.015 ± 0.336
2.905ValPro: 2.905 ± 0.333
2.28ValGln: 2.28 ± 0.268
3.934ValArg: 3.934 ± 0.364
4.044ValSer: 4.044 ± 0.36
4.302ValThr: 4.302 ± 0.373
5.92ValVal: 5.92 ± 0.547
1.213ValTrp: 1.213 ± 0.237
2.537ValTyr: 2.537 ± 0.368
0.0ValXaa: 0.0 ± 0.0
Trp
1.029TrpAla: 1.029 ± 0.175
0.404TrpCys: 0.404 ± 0.115
1.177TrpAsp: 1.177 ± 0.197
1.434TrpGlu: 1.434 ± 0.203
0.699TrpPhe: 0.699 ± 0.149
0.956TrpGly: 0.956 ± 0.195
0.441TrpHis: 0.441 ± 0.112
0.882TrpIle: 0.882 ± 0.173
1.103TrpLys: 1.103 ± 0.207
1.471TrpLeu: 1.471 ± 0.229
0.699TrpMet: 0.699 ± 0.165
0.809TrpAsn: 0.809 ± 0.164
0.552TrpPro: 0.552 ± 0.141
0.552TrpGln: 0.552 ± 0.139
1.177TrpArg: 1.177 ± 0.218
1.029TrpSer: 1.029 ± 0.207
0.993TrpThr: 0.993 ± 0.206
1.36TrpVal: 1.36 ± 0.248
0.294TrpTrp: 0.294 ± 0.082
0.662TrpTyr: 0.662 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.758TyrAla: 2.758 ± 0.345
0.625TyrCys: 0.625 ± 0.187
2.243TyrAsp: 2.243 ± 0.287
2.463TyrGlu: 2.463 ± 0.357
1.765TyrPhe: 1.765 ± 0.225
2.831TyrGly: 2.831 ± 0.32
0.441TyrHis: 0.441 ± 0.135
2.463TyrIle: 2.463 ± 0.322
2.574TyrLys: 2.574 ± 0.332
2.463TyrLeu: 2.463 ± 0.271
1.103TyrMet: 1.103 ± 0.22
1.985TyrAsn: 1.985 ± 0.264
1.471TyrPro: 1.471 ± 0.211
1.471TyrGln: 1.471 ± 0.218
2.133TyrArg: 2.133 ± 0.276
2.463TyrSer: 2.463 ± 0.357
2.463TyrThr: 2.463 ± 0.322
2.39TyrVal: 2.39 ± 0.298
0.404TyrTrp: 0.404 ± 0.121
1.36TyrTyr: 1.36 ± 0.222
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 171 proteins (27199 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski