Podoviridae sp. ctviO18

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W4G5|A0A5Q2W4G5_9CAUD Uncharacterized protein OS=Podoviridae sp. ctviO18 OX=2656716 PE=4 SV=1
MM1 pKa = 7.4MKK3 pKa = 9.76MKK5 pKa = 10.54NRR7 pKa = 11.84TFLIFPIISVGLIVIALLGIQIVYY31 pKa = 10.81APVQTVKK38 pKa = 10.95NLTAGDD44 pKa = 4.1TIGTLPAIIASGSRR58 pKa = 11.84NLLVPRR64 pKa = 11.84DD65 pKa = 3.6RR66 pKa = 11.84DD67 pKa = 3.21ADD69 pKa = 3.97FVFGGQSSVSAQGKK83 pKa = 8.84FVLDD87 pKa = 4.01DD88 pKa = 3.62VGSTMNLEE96 pKa = 4.28LDD98 pKa = 4.85GIASISKK105 pKa = 10.19FSLIDD110 pKa = 3.34SSTGITMVDD119 pKa = 3.54CDD121 pKa = 4.88GDD123 pKa = 3.96AQALGWDD130 pKa = 3.75LTNQRR135 pKa = 11.84FFCGDD140 pKa = 3.63DD141 pKa = 3.48DD142 pKa = 4.83SGGGGGGTLIEE153 pKa = 4.32VQNSFVDD160 pKa = 3.84LGSFSSISFDD170 pKa = 3.3AGHH173 pKa = 6.1FTATDD178 pKa = 3.5TTGEE182 pKa = 4.04ATIKK186 pKa = 10.52LDD188 pKa = 3.22WGAGGPASLSQAEE201 pKa = 4.73TITGNWVNTDD211 pKa = 3.76NPWDD215 pKa = 3.53ISTEE219 pKa = 4.21TNLTAGTDD227 pKa = 3.62LTLTNDD233 pKa = 4.18DD234 pKa = 4.18LTLDD238 pKa = 3.63STLTQNFTFNAAGTAVIVTNNARR261 pKa = 11.84FGTASVSTGFEE272 pKa = 4.49AITYY276 pKa = 9.84ASASLFQGSAFAGIPGAEE294 pKa = 4.5CSDD297 pKa = 4.6AGDD300 pKa = 3.61TLAWANGQFTCGSDD314 pKa = 3.12ASGSGGASVEE324 pKa = 4.26FQEE327 pKa = 5.18SGTDD331 pKa = 3.04KK332 pKa = 11.35GSFSSVSFDD341 pKa = 2.95AGKK344 pKa = 10.77FNITDD349 pKa = 3.41TTGEE353 pKa = 3.88VDD355 pKa = 5.49IDD357 pKa = 3.78IDD359 pKa = 3.72WTSGGGPASKK369 pKa = 10.25SQANTWSQLQTFTLGASSTLNFEE392 pKa = 4.36AVGYY396 pKa = 10.49ASASALLGSAFTSVGDD412 pKa = 4.26CNDD415 pKa = 3.08ATEE418 pKa = 5.02AIGWTTTGIFNCRR431 pKa = 11.84SVQDD435 pKa = 4.02LDD437 pKa = 3.76STLTALAAYY446 pKa = 7.33NTNGLLTQTAADD458 pKa = 3.96TFTGRR463 pKa = 11.84TITGTSNQITVANGDD478 pKa = 3.98GVSGNPTLSIPSIFIAPGTASVTTDD503 pKa = 3.61FEE505 pKa = 6.01AIGFASASAFRR516 pKa = 11.84GSAFSGGDD524 pKa = 3.58CDD526 pKa = 5.48GATQKK531 pKa = 9.47LTWDD535 pKa = 3.65TTNGTFGCATDD546 pKa = 4.51DD547 pKa = 4.13DD548 pKa = 4.58VPEE551 pKa = 4.43VGDD554 pKa = 4.29FGALVGGLAIDD565 pKa = 4.2NNSGTLDD572 pKa = 4.21LDD574 pKa = 3.42PTEE577 pKa = 5.32LINNRR582 pKa = 11.84TWSDD586 pKa = 3.44GTSDD590 pKa = 5.0SSIVWTWNLSAGTDD604 pKa = 3.51PTLTFGDD611 pKa = 4.39GVITFGQGATISTNFEE627 pKa = 4.16VKK629 pKa = 9.08GTASISAFTLPDD641 pKa = 3.51KK642 pKa = 11.36DD643 pKa = 3.63GGTLGDD649 pKa = 4.65CEE651 pKa = 4.55ASTEE655 pKa = 4.14KK656 pKa = 10.71VVYY659 pKa = 10.59DD660 pKa = 4.12LATKK664 pKa = 10.61KK665 pKa = 10.27FDD667 pKa = 4.21CGTDD671 pKa = 3.36QSGSGGSGGNVSDD684 pKa = 4.89GLDD687 pKa = 3.12IANSGGTYY695 pKa = 10.31LAIASLSFDD704 pKa = 3.21ASHH707 pKa = 6.48FTFTNTASDD716 pKa = 4.26GYY718 pKa = 10.67IRR720 pKa = 11.84LDD722 pKa = 3.31WGSGGPASLSEE733 pKa = 4.49AEE735 pKa = 4.47TISGNWVNTASPWADD750 pKa = 3.06NEE752 pKa = 4.39VVDD755 pKa = 5.09TISIIGGIIGANSISGTQTTTGTLTIGDD783 pKa = 4.29NGDD786 pKa = 4.05SIIIDD791 pKa = 3.52ASNWDD796 pKa = 3.51VSTLGKK802 pKa = 10.5ADD804 pKa = 3.93FLSASVSTNFEE815 pKa = 3.79ATGYY819 pKa = 10.88ASASKK824 pKa = 10.88YY825 pKa = 10.43FGAGLGSMSGTDD837 pKa = 3.46GCSAAGDD844 pKa = 3.78TLNYY848 pKa = 8.98TFSTGVFSCGSDD860 pKa = 2.91ASGGGGVSSNSLNFDD875 pKa = 3.43EE876 pKa = 5.08FQNPLVLDD884 pKa = 3.63VMASVQFGVFNWEE897 pKa = 3.47FDD899 pKa = 4.09LNSTGDD905 pKa = 4.48FIISDD910 pKa = 3.89NNSNLVTFWDD920 pKa = 3.45TGGASFSTPVTGTIDD935 pKa = 3.34EE936 pKa = 4.99LLTFGATSAIPPSTSFATFDD956 pKa = 3.34TRR958 pKa = 11.84NDD960 pKa = 3.32FTVLDD965 pKa = 4.28FGDD968 pKa = 3.76GAVASKK974 pKa = 10.82SIFVGVMPRR983 pKa = 11.84DD984 pKa = 3.69YY985 pKa = 11.49DD986 pKa = 4.12DD987 pKa = 3.44GTMTIVIHH995 pKa = 6.23FAATSATSGNAVWDD1009 pKa = 3.84VEE1011 pKa = 4.0FDD1013 pKa = 3.8RR1014 pKa = 11.84VGTTLDD1020 pKa = 3.31TDD1022 pKa = 3.89SASWATARR1030 pKa = 11.84TATCAVSGTAGRR1042 pKa = 11.84LSTCLITFTKK1052 pKa = 10.37AQADD1056 pKa = 3.99GVLKK1060 pKa = 11.12GEE1062 pKa = 4.66LFRR1065 pKa = 11.84MRR1067 pKa = 11.84VSRR1070 pKa = 11.84DD1071 pKa = 3.12TTDD1074 pKa = 3.35AADD1077 pKa = 3.74TVTGTDD1083 pKa = 3.22LEE1085 pKa = 4.5LYY1087 pKa = 9.89YY1088 pKa = 10.11IEE1090 pKa = 5.8IIQQ1093 pKa = 4.02

Molecular weight: 112.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WAV3|A0A5Q2WAV3_9CAUD Uncharacterized protein OS=Podoviridae sp. ctviO18 OX=2656716 PE=4 SV=1
MM1 pKa = 7.31PQAFEE6 pKa = 3.74QCRR9 pKa = 11.84ANGGKK14 pKa = 9.24IRR16 pKa = 11.84TKK18 pKa = 9.47TLPGGKK24 pKa = 8.98YY25 pKa = 7.11VHH27 pKa = 6.79FCILNGKK34 pKa = 7.69SYY36 pKa = 10.96RR37 pKa = 11.84GYY39 pKa = 9.14TKK41 pKa = 9.09TARR44 pKa = 11.84TSRR47 pKa = 11.84YY48 pKa = 8.61KK49 pKa = 10.87GRR51 pKa = 11.84SSEE54 pKa = 4.33SKK56 pKa = 10.97SNNQYY61 pKa = 11.3

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11718

30

1238

202.0

22.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.075 ± 0.418

1.135 ± 0.191

6.298 ± 0.381

6.281 ± 0.559

3.951 ± 0.241

8.679 ± 0.752

1.434 ± 0.196

6.494 ± 0.241

7.937 ± 0.799

7.518 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.005 ± 0.198

5.069 ± 0.227

3.849 ± 0.29

3.354 ± 0.216

4.642 ± 0.395

6.93 ± 0.518

6.4 ± 0.649

5.863 ± 0.256

1.63 ± 0.185

3.456 ± 0.367
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski