Amino acid dipepetide frequency for Podoviridae sp. ctviO18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.353AlaAla: 4.353 ± 0.7
0.427AlaCys: 0.427 ± 0.175
3.328AlaAsp: 3.328 ± 0.486
3.585AlaGlu: 3.585 ± 0.606
2.987AlaPhe: 2.987 ± 0.499
6.998AlaGly: 6.998 ± 1.18
0.597AlaHis: 0.597 ± 0.241
4.523AlaIle: 4.523 ± 0.602
4.95AlaLys: 4.95 ± 0.881
5.974AlaLeu: 5.974 ± 0.618
1.28AlaMet: 1.28 ± 0.378
3.755AlaAsn: 3.755 ± 0.471
2.134AlaPro: 2.134 ± 0.522
2.987AlaGln: 2.987 ± 0.508
3.158AlaArg: 3.158 ± 0.633
7.937AlaSer: 7.937 ± 1.778
4.865AlaThr: 4.865 ± 0.889
4.438AlaVal: 4.438 ± 0.459
1.28AlaTrp: 1.28 ± 0.334
1.195AlaTyr: 1.195 ± 0.42
0.0AlaXaa: 0.0 ± 0.0
Cys
0.341CysAla: 0.341 ± 0.194
0.085CysCys: 0.085 ± 0.097
0.939CysAsp: 0.939 ± 0.269
0.768CysGlu: 0.768 ± 0.226
0.256CysPhe: 0.256 ± 0.159
1.878CysGly: 1.878 ± 0.524
0.427CysHis: 0.427 ± 0.189
0.683CysIle: 0.683 ± 0.237
0.085CysLys: 0.085 ± 0.09
1.28CysLeu: 1.28 ± 0.293
0.0CysMet: 0.0 ± 0.0
0.427CysAsn: 0.427 ± 0.174
0.768CysPro: 0.768 ± 0.262
0.256CysGln: 0.256 ± 0.191
1.024CysArg: 1.024 ± 0.324
0.853CysSer: 0.853 ± 0.267
0.427CysThr: 0.427 ± 0.208
0.341CysVal: 0.341 ± 0.158
0.085CysTrp: 0.085 ± 0.097
0.427CysTyr: 0.427 ± 0.196
0.0CysXaa: 0.0 ± 0.0
Asp
3.755AspAla: 3.755 ± 0.603
0.853AspCys: 0.853 ± 0.355
4.523AspAsp: 4.523 ± 0.673
4.267AspGlu: 4.267 ± 0.973
2.902AspPhe: 2.902 ± 0.566
5.804AspGly: 5.804 ± 0.993
0.683AspHis: 0.683 ± 0.232
5.035AspIle: 5.035 ± 0.678
4.438AspLys: 4.438 ± 0.747
4.097AspLeu: 4.097 ± 0.584
1.451AspMet: 1.451 ± 0.377
2.56AspAsn: 2.56 ± 0.461
2.048AspPro: 2.048 ± 0.434
2.475AspGln: 2.475 ± 0.475
2.134AspArg: 2.134 ± 0.354
4.011AspSer: 4.011 ± 0.524
3.926AspThr: 3.926 ± 0.672
4.097AspVal: 4.097 ± 0.595
1.366AspTrp: 1.366 ± 0.344
2.56AspTyr: 2.56 ± 0.538
0.0AspXaa: 0.0 ± 0.0
Glu
4.353GluAla: 4.353 ± 0.699
0.597GluCys: 0.597 ± 0.212
4.182GluAsp: 4.182 ± 0.88
5.718GluGlu: 5.718 ± 1.132
3.414GluPhe: 3.414 ± 0.545
4.011GluGly: 4.011 ± 0.743
1.109GluHis: 1.109 ± 0.373
4.694GluIle: 4.694 ± 0.625
5.121GluLys: 5.121 ± 0.851
5.206GluLeu: 5.206 ± 0.612
1.109GluMet: 1.109 ± 0.319
2.731GluAsn: 2.731 ± 0.463
1.963GluPro: 1.963 ± 0.521
2.219GluGln: 2.219 ± 0.581
2.475GluArg: 2.475 ± 0.505
3.414GluSer: 3.414 ± 0.626
3.243GluThr: 3.243 ± 0.563
3.755GluVal: 3.755 ± 0.473
0.853GluTrp: 0.853 ± 0.292
2.646GluTyr: 2.646 ± 0.603
0.0GluXaa: 0.0 ± 0.0
Phe
2.731PheAla: 2.731 ± 0.519
0.683PheCys: 0.683 ± 0.257
3.328PheAsp: 3.328 ± 0.546
2.902PheGlu: 2.902 ± 0.484
1.109PhePhe: 1.109 ± 0.307
3.072PheGly: 3.072 ± 0.725
0.853PheHis: 0.853 ± 0.253
2.646PheIle: 2.646 ± 0.469
2.39PheLys: 2.39 ± 0.556
2.219PheLeu: 2.219 ± 0.369
0.683PheMet: 0.683 ± 0.246
2.219PheAsn: 2.219 ± 0.389
1.195PhePro: 1.195 ± 0.288
0.939PheGln: 0.939 ± 0.284
1.963PheArg: 1.963 ± 0.394
2.987PheSer: 2.987 ± 0.542
2.902PheThr: 2.902 ± 0.68
2.816PheVal: 2.816 ± 0.468
0.597PheTrp: 0.597 ± 0.192
1.28PheTyr: 1.28 ± 0.372
0.0PheXaa: 0.0 ± 0.0
Gly
6.23GlyAla: 6.23 ± 1.563
0.939GlyCys: 0.939 ± 0.356
5.633GlyAsp: 5.633 ± 0.961
4.779GlyGlu: 4.779 ± 0.775
2.816GlyPhe: 2.816 ± 0.413
11.607GlyGly: 11.607 ± 3.259
1.28GlyHis: 1.28 ± 0.362
5.718GlyIle: 5.718 ± 0.567
5.889GlyLys: 5.889 ± 0.868
6.145GlyLeu: 6.145 ± 0.699
0.853GlyMet: 0.853 ± 0.32
3.841GlyAsn: 3.841 ± 0.689
1.195GlyPro: 1.195 ± 0.268
2.731GlyGln: 2.731 ± 0.365
4.097GlyArg: 4.097 ± 0.574
5.547GlySer: 5.547 ± 0.973
8.535GlyThr: 8.535 ± 1.759
4.353GlyVal: 4.353 ± 0.52
1.28GlyTrp: 1.28 ± 0.311
3.328GlyTyr: 3.328 ± 0.662
0.0GlyXaa: 0.0 ± 0.0
His
0.853HisAla: 0.853 ± 0.249
0.085HisCys: 0.085 ± 0.084
0.768HisAsp: 0.768 ± 0.262
0.768HisGlu: 0.768 ± 0.303
1.451HisPhe: 1.451 ± 0.331
0.853HisGly: 0.853 ± 0.228
0.256HisHis: 0.256 ± 0.154
0.768HisIle: 0.768 ± 0.306
1.109HisLys: 1.109 ± 0.299
1.28HisLeu: 1.28 ± 0.34
0.171HisMet: 0.171 ± 0.101
0.853HisAsn: 0.853 ± 0.306
0.597HisPro: 0.597 ± 0.238
0.512HisGln: 0.512 ± 0.253
1.109HisArg: 1.109 ± 0.331
0.683HisSer: 0.683 ± 0.214
0.341HisThr: 0.341 ± 0.16
0.341HisVal: 0.341 ± 0.153
0.341HisTrp: 0.341 ± 0.162
1.195HisTyr: 1.195 ± 0.358
0.0HisXaa: 0.0 ± 0.0
Ile
4.097IleAla: 4.097 ± 0.601
0.427IleCys: 0.427 ± 0.208
4.95IleAsp: 4.95 ± 0.641
3.755IleGlu: 3.755 ± 0.596
2.134IlePhe: 2.134 ± 0.397
4.609IleGly: 4.609 ± 0.541
1.366IleHis: 1.366 ± 0.319
4.011IleIle: 4.011 ± 0.686
6.145IleLys: 6.145 ± 0.915
4.267IleLeu: 4.267 ± 0.588
1.451IleMet: 1.451 ± 0.432
3.328IleAsn: 3.328 ± 0.571
3.499IlePro: 3.499 ± 0.546
2.39IleGln: 2.39 ± 0.5
3.414IleArg: 3.414 ± 0.618
3.926IleSer: 3.926 ± 0.642
4.694IleThr: 4.694 ± 0.619
3.499IleVal: 3.499 ± 0.441
0.939IleTrp: 0.939 ± 0.29
2.048IleTyr: 2.048 ± 0.48
0.0IleXaa: 0.0 ± 0.0
Lys
4.011LysAla: 4.011 ± 0.774
1.024LysCys: 1.024 ± 0.398
4.523LysAsp: 4.523 ± 0.764
5.974LysGlu: 5.974 ± 1.09
2.304LysPhe: 2.304 ± 0.445
5.718LysGly: 5.718 ± 0.858
1.024LysHis: 1.024 ± 0.301
5.974LysIle: 5.974 ± 1.012
6.828LysLys: 6.828 ± 1.295
6.145LysLeu: 6.145 ± 0.856
2.475LysMet: 2.475 ± 0.441
4.523LysAsn: 4.523 ± 0.552
3.499LysPro: 3.499 ± 0.506
3.072LysGln: 3.072 ± 0.477
3.072LysArg: 3.072 ± 0.588
4.865LysSer: 4.865 ± 0.755
5.035LysThr: 5.035 ± 0.788
5.377LysVal: 5.377 ± 0.834
1.024LysTrp: 1.024 ± 0.295
2.902LysTyr: 2.902 ± 0.465
0.0LysXaa: 0.0 ± 0.0
Leu
5.121LeuAla: 5.121 ± 0.785
0.597LeuCys: 0.597 ± 0.252
4.267LeuAsp: 4.267 ± 0.788
4.779LeuGlu: 4.779 ± 0.742
2.816LeuPhe: 2.816 ± 0.473
5.633LeuGly: 5.633 ± 0.612
0.768LeuHis: 0.768 ± 0.275
4.097LeuIle: 4.097 ± 0.52
7.51LeuLys: 7.51 ± 0.97
5.633LeuLeu: 5.633 ± 0.795
2.219LeuMet: 2.219 ± 0.476
4.011LeuAsn: 4.011 ± 0.491
3.926LeuPro: 3.926 ± 0.581
1.792LeuGln: 1.792 ± 0.462
2.646LeuArg: 2.646 ± 0.511
5.121LeuSer: 5.121 ± 0.813
5.804LeuThr: 5.804 ± 0.811
5.121LeuVal: 5.121 ± 0.619
1.195LeuTrp: 1.195 ± 0.36
2.134LeuTyr: 2.134 ± 0.433
0.0LeuXaa: 0.0 ± 0.0
Met
1.878MetAla: 1.878 ± 0.356
0.171MetCys: 0.171 ± 0.117
1.366MetAsp: 1.366 ± 0.401
1.195MetGlu: 1.195 ± 0.375
0.597MetPhe: 0.597 ± 0.26
1.451MetGly: 1.451 ± 0.342
0.171MetHis: 0.171 ± 0.113
1.28MetIle: 1.28 ± 0.303
2.731MetLys: 2.731 ± 0.472
0.683MetLeu: 0.683 ± 0.236
0.256MetMet: 0.256 ± 0.115
1.024MetAsn: 1.024 ± 0.251
1.366MetPro: 1.366 ± 0.304
0.512MetGln: 0.512 ± 0.189
1.536MetArg: 1.536 ± 0.353
1.109MetSer: 1.109 ± 0.324
1.109MetThr: 1.109 ± 0.31
0.768MetVal: 0.768 ± 0.202
0.427MetTrp: 0.427 ± 0.189
0.427MetTyr: 0.427 ± 0.351
0.0MetXaa: 0.0 ± 0.0
Asn
3.328AsnAla: 3.328 ± 0.471
0.853AsnCys: 0.853 ± 0.262
1.451AsnAsp: 1.451 ± 0.284
2.304AsnGlu: 2.304 ± 0.443
2.475AsnPhe: 2.475 ± 0.388
5.377AsnGly: 5.377 ± 0.693
0.683AsnHis: 0.683 ± 0.209
3.585AsnIle: 3.585 ± 0.642
4.95AsnLys: 4.95 ± 0.808
3.414AsnLeu: 3.414 ± 0.475
0.853AsnMet: 0.853 ± 0.262
3.328AsnAsn: 3.328 ± 0.574
2.56AsnPro: 2.56 ± 0.383
1.28AsnGln: 1.28 ± 0.313
3.243AsnArg: 3.243 ± 0.58
2.816AsnSer: 2.816 ± 0.626
2.475AsnThr: 2.475 ± 0.519
2.56AsnVal: 2.56 ± 0.482
1.536AsnTrp: 1.536 ± 0.418
1.622AsnTyr: 1.622 ± 0.358
0.0AsnXaa: 0.0 ± 0.0
Pro
2.475ProAla: 2.475 ± 0.37
0.0ProCys: 0.0 ± 0.0
3.072ProAsp: 3.072 ± 0.629
3.414ProGlu: 3.414 ± 0.686
1.195ProPhe: 1.195 ± 0.288
1.963ProGly: 1.963 ± 0.343
0.341ProHis: 0.341 ± 0.149
1.536ProIle: 1.536 ± 0.313
3.499ProLys: 3.499 ± 0.638
2.987ProLeu: 2.987 ± 0.585
0.683ProMet: 0.683 ± 0.245
2.475ProAsn: 2.475 ± 0.476
1.195ProPro: 1.195 ± 0.313
1.195ProGln: 1.195 ± 0.319
2.475ProArg: 2.475 ± 0.474
2.56ProSer: 2.56 ± 0.468
2.902ProThr: 2.902 ± 0.549
2.475ProVal: 2.475 ± 0.399
0.171ProTrp: 0.171 ± 0.134
1.878ProTyr: 1.878 ± 0.424
0.0ProXaa: 0.0 ± 0.0
Gln
3.072GlnAla: 3.072 ± 0.402
0.512GlnCys: 0.512 ± 0.22
1.622GlnAsp: 1.622 ± 0.315
2.56GlnGlu: 2.56 ± 0.445
1.622GlnPhe: 1.622 ± 0.393
2.56GlnGly: 2.56 ± 0.445
0.512GlnHis: 0.512 ± 0.244
2.134GlnIle: 2.134 ± 0.33
2.304GlnLys: 2.304 ± 0.479
2.987GlnLeu: 2.987 ± 0.464
0.768GlnMet: 0.768 ± 0.223
1.878GlnAsn: 1.878 ± 0.432
1.109GlnPro: 1.109 ± 0.289
1.024GlnGln: 1.024 ± 0.286
1.878GlnArg: 1.878 ± 0.333
2.219GlnSer: 2.219 ± 0.436
1.622GlnThr: 1.622 ± 0.345
1.792GlnVal: 1.792 ± 0.313
0.512GlnTrp: 0.512 ± 0.203
0.853GlnTyr: 0.853 ± 0.29
0.0GlnXaa: 0.0 ± 0.0
Arg
3.158ArgAla: 3.158 ± 0.655
0.939ArgCys: 0.939 ± 0.341
3.585ArgAsp: 3.585 ± 0.74
2.304ArgGlu: 2.304 ± 0.452
1.707ArgPhe: 1.707 ± 0.56
2.902ArgGly: 2.902 ± 0.47
0.683ArgHis: 0.683 ± 0.198
3.499ArgIle: 3.499 ± 0.619
3.072ArgLys: 3.072 ± 0.609
3.243ArgLeu: 3.243 ± 0.552
2.304ArgMet: 2.304 ± 0.363
2.987ArgAsn: 2.987 ± 0.567
1.963ArgPro: 1.963 ± 0.415
1.622ArgGln: 1.622 ± 0.42
1.878ArgArg: 1.878 ± 0.46
2.816ArgSer: 2.816 ± 0.468
2.731ArgThr: 2.731 ± 0.509
2.646ArgVal: 2.646 ± 0.422
0.683ArgTrp: 0.683 ± 0.3
1.707ArgTyr: 1.707 ± 0.433
0.0ArgXaa: 0.0 ± 0.0
Ser
5.889SerAla: 5.889 ± 1.064
1.109SerCys: 1.109 ± 0.261
3.499SerAsp: 3.499 ± 0.593
3.414SerGlu: 3.414 ± 0.495
2.134SerPhe: 2.134 ± 0.5
8.023SerGly: 8.023 ± 1.331
0.939SerHis: 0.939 ± 0.257
4.523SerIle: 4.523 ± 0.749
5.547SerLys: 5.547 ± 0.553
5.035SerLeu: 5.035 ± 0.636
1.109SerMet: 1.109 ± 0.239
2.902SerAsn: 2.902 ± 0.505
3.158SerPro: 3.158 ± 0.537
2.56SerGln: 2.56 ± 0.512
2.987SerArg: 2.987 ± 0.511
4.865SerSer: 4.865 ± 0.722
4.182SerThr: 4.182 ± 1.107
3.926SerVal: 3.926 ± 0.597
1.536SerTrp: 1.536 ± 0.344
1.963SerTyr: 1.963 ± 0.497
0.0SerXaa: 0.0 ± 0.0
Thr
6.316ThrAla: 6.316 ± 1.058
0.683ThrCys: 0.683 ± 0.235
4.694ThrAsp: 4.694 ± 0.701
3.328ThrGlu: 3.328 ± 0.463
3.072ThrPhe: 3.072 ± 0.818
6.913ThrGly: 6.913 ± 1.2
1.109ThrHis: 1.109 ± 0.329
3.585ThrIle: 3.585 ± 0.728
4.182ThrLys: 4.182 ± 0.637
5.121ThrLeu: 5.121 ± 0.997
0.768ThrMet: 0.768 ± 0.258
3.328ThrAsn: 3.328 ± 0.599
2.39ThrPro: 2.39 ± 0.406
1.878ThrGln: 1.878 ± 0.328
1.707ThrArg: 1.707 ± 0.436
4.097ThrSer: 4.097 ± 0.782
4.353ThrThr: 4.353 ± 0.647
3.328ThrVal: 3.328 ± 0.564
0.683ThrTrp: 0.683 ± 0.271
2.475ThrTyr: 2.475 ± 0.371
0.0ThrXaa: 0.0 ± 0.0
Val
5.121ValAla: 5.121 ± 0.827
0.768ValCys: 0.768 ± 0.25
4.097ValAsp: 4.097 ± 0.556
3.328ValGlu: 3.328 ± 0.485
2.475ValPhe: 2.475 ± 0.427
3.926ValGly: 3.926 ± 0.518
0.768ValHis: 0.768 ± 0.275
3.585ValIle: 3.585 ± 0.453
4.95ValLys: 4.95 ± 0.639
4.694ValLeu: 4.694 ± 0.536
1.28ValMet: 1.28 ± 0.346
2.304ValAsn: 2.304 ± 0.48
2.56ValPro: 2.56 ± 0.362
1.536ValGln: 1.536 ± 0.315
2.56ValArg: 2.56 ± 0.638
5.291ValSer: 5.291 ± 0.706
1.792ValThr: 1.792 ± 0.447
4.523ValVal: 4.523 ± 0.857
0.768ValTrp: 0.768 ± 0.24
2.304ValTyr: 2.304 ± 0.373
0.0ValXaa: 0.0 ± 0.0
Trp
1.451TrpAla: 1.451 ± 0.341
0.0TrpCys: 0.0 ± 0.0
1.28TrpAsp: 1.28 ± 0.376
1.109TrpGlu: 1.109 ± 0.286
0.597TrpPhe: 0.597 ± 0.281
1.28TrpGly: 1.28 ± 0.27
0.085TrpHis: 0.085 ± 0.064
1.792TrpIle: 1.792 ± 0.395
1.024TrpLys: 1.024 ± 0.273
1.622TrpLeu: 1.622 ± 0.391
0.0TrpMet: 0.0 ± 0.0
0.768TrpAsn: 0.768 ± 0.262
0.427TrpPro: 0.427 ± 0.224
0.683TrpGln: 0.683 ± 0.25
0.853TrpArg: 0.853 ± 0.349
1.366TrpSer: 1.366 ± 0.264
0.768TrpThr: 0.768 ± 0.244
0.597TrpVal: 0.597 ± 0.21
0.256TrpTrp: 0.256 ± 0.148
0.341TrpTyr: 0.341 ± 0.209
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.219TyrAla: 2.219 ± 0.359
0.597TyrCys: 0.597 ± 0.245
1.878TyrAsp: 1.878 ± 0.421
2.56TyrGlu: 2.56 ± 0.669
1.451TyrPhe: 1.451 ± 0.369
2.219TyrGly: 2.219 ± 0.456
0.683TyrHis: 0.683 ± 0.314
1.28TyrIle: 1.28 ± 0.314
2.646TyrLys: 2.646 ± 0.502
3.158TyrLeu: 3.158 ± 0.584
0.256TyrMet: 0.256 ± 0.137
1.451TyrAsn: 1.451 ± 0.396
0.939TyrPro: 0.939 ± 0.303
1.878TyrGln: 1.878 ± 0.385
2.219TyrArg: 2.219 ± 0.516
2.816TyrSer: 2.816 ± 0.491
2.304TyrThr: 2.304 ± 0.389
1.878TyrVal: 1.878 ± 0.374
0.768TyrTrp: 0.768 ± 0.301
1.28TyrTyr: 1.28 ± 0.269
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (11718 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski