Caenispirillum salinarum AK4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Caenispirillum; Caenispirillum salinarum

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4574 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9GRX7|K9GRX7_9PROT Alpha-1 4 glucan phosphorylase OS=Caenispirillum salinarum AK4 OX=1238182 GN=C882_0927 PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.22KK3 pKa = 10.34VLLGTSALIAAAAISAPAMAQEE25 pKa = 4.21QIQLGIGGKK34 pKa = 7.61MEE36 pKa = 4.03QYY38 pKa = 10.72FGVTSIDD45 pKa = 3.43DD46 pKa = 3.85DD47 pKa = 4.12AAGFDD52 pKa = 3.94PTTTGINTDD61 pKa = 3.17AEE63 pKa = 5.03VYY65 pKa = 8.13FTGATTLDD73 pKa = 3.25NGLTVGAVIQLEE85 pKa = 4.33AQTNANVTFNADD97 pKa = 2.86EE98 pKa = 3.99QYY100 pKa = 11.54AYY102 pKa = 9.92IEE104 pKa = 4.36GAFGQLRR111 pKa = 11.84AGQKK115 pKa = 10.4NGILQDD121 pKa = 4.03LAHH124 pKa = 6.91TAPQIGISFLDD135 pKa = 3.6AASFVPSAGLAVGTSQSKK153 pKa = 10.49LVDD156 pKa = 3.47TTVSDD161 pKa = 4.34DD162 pKa = 3.82SASVSYY168 pKa = 8.87ITPTLAGFSAGVTFNPNPGGALQANQEE195 pKa = 4.47DD196 pKa = 4.82GAGTHH201 pKa = 6.33NKK203 pKa = 9.14FQVGLAYY210 pKa = 10.57NGEE213 pKa = 4.06FSGVGIGVDD222 pKa = 3.55GSYY225 pKa = 8.18QTEE228 pKa = 4.11EE229 pKa = 4.17ADD231 pKa = 3.93DD232 pKa = 5.41AIVGADD238 pKa = 4.59DD239 pKa = 3.59PTVWRR244 pKa = 11.84AGLNLAYY251 pKa = 10.21AGFQAGGSYY260 pKa = 10.31LRR262 pKa = 11.84SEE264 pKa = 4.39DD265 pKa = 3.72TADD268 pKa = 3.67TEE270 pKa = 4.6TTTWDD275 pKa = 2.94AGVAYY280 pKa = 9.84GVGPYY285 pKa = 9.45QVGVGYY291 pKa = 10.47VQTEE295 pKa = 4.01VDD297 pKa = 4.71DD298 pKa = 4.2GTVANDD304 pKa = 2.75VDD306 pKa = 4.59EE307 pKa = 4.31YY308 pKa = 11.33RR309 pKa = 11.84QVQLSGSYY317 pKa = 10.03QMGPGIKK324 pKa = 10.02AVGGLFWYY332 pKa = 10.0EE333 pKa = 3.98SEE335 pKa = 4.69SNVGLGTTTTNEE347 pKa = 3.71RR348 pKa = 11.84DD349 pKa = 3.41GGGGIVGLALTFF361 pKa = 4.17

Molecular weight: 37.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9GT92|K9GT92_9PROT Tryptophan-rich sensory protein OS=Caenispirillum salinarum AK4 OX=1238182 GN=C882_0981 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84SATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLANRR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.48GRR39 pKa = 11.84KK40 pKa = 8.72RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4574

0

4574

1438899

37

5007

314.6

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.233 ± 0.067

0.875 ± 0.011

6.299 ± 0.036

6.064 ± 0.036

3.28 ± 0.023

8.844 ± 0.036

2.145 ± 0.019

4.211 ± 0.025

2.807 ± 0.03

10.069 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.635 ± 0.018

2.183 ± 0.022

5.652 ± 0.032

2.807 ± 0.022

7.514 ± 0.042

4.454 ± 0.023

5.537 ± 0.034

7.992 ± 0.033

1.363 ± 0.017

2.035 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski