Akanthomyces lecanii RCEF 1005

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Cordycipitaceae; Akanthomyces; Akanthomyces lecanii

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11019 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A168H3Q9|A0A168H3Q9_CORDF Uncharacterized protein OS=Akanthomyces lecanii RCEF 1005 OX=1081108 GN=LEL_04094 PE=4 SV=1
MM1 pKa = 7.46FLQTILAATAFGALASAAPTQSRR24 pKa = 11.84STGGKK29 pKa = 9.24LAVYY33 pKa = 9.01WGAEE37 pKa = 4.13DD38 pKa = 5.16SSTTLDD44 pKa = 4.07DD45 pKa = 3.66VCGDD49 pKa = 3.4SSYY52 pKa = 11.43DD53 pKa = 3.44IVNLAFLSYY62 pKa = 9.85FFKK65 pKa = 10.99DD66 pKa = 2.77GGYY69 pKa = 9.51PEE71 pKa = 5.71LSISTLGGPSDD82 pKa = 3.79AQQAAGATSLQDD94 pKa = 3.36GSEE97 pKa = 4.23LVPAIQKK104 pKa = 8.28CQKK107 pKa = 9.17NGKK110 pKa = 9.24RR111 pKa = 11.84VILSMGGAQGYY122 pKa = 11.19ADD124 pKa = 3.64VTLQSDD130 pKa = 3.66AQGQQIAQTIWDD142 pKa = 4.43LFLGGTNNPSLRR154 pKa = 11.84PFGDD158 pKa = 3.28VKK160 pKa = 11.22LDD162 pKa = 4.15GVDD165 pKa = 4.26LDD167 pKa = 4.54NEE169 pKa = 4.72SGNPTGYY176 pKa = 11.11VAMTKK181 pKa = 7.8QFRR184 pKa = 11.84SLMNSDD190 pKa = 3.38SSKK193 pKa = 10.95QYY195 pKa = 11.27VLTAAPQCPFPDD207 pKa = 3.44ASEE210 pKa = 4.26PLDD213 pKa = 3.71VVQLLDD219 pKa = 3.79YY220 pKa = 11.14VWVQFYY226 pKa = 11.57NNGDD230 pKa = 3.84CNIAQSGFNDD240 pKa = 4.13AVRR243 pKa = 11.84TWSQGIGNATLFIGALASGADD264 pKa = 3.63GDD266 pKa = 3.91QGYY269 pKa = 10.73VDD271 pKa = 4.81ASTFTSALQGVEE283 pKa = 4.45AMNLPNYY290 pKa = 8.91GGAMLWEE297 pKa = 4.42AQLAVNNGNYY307 pKa = 7.98QQQIRR312 pKa = 11.84GSLL315 pKa = 3.3

Molecular weight: 33.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A168CSM9|A0A168CSM9_CORDF Ferredoxin reductase-type FAD-binding domain protein OS=Akanthomyces lecanii RCEF 1005 OX=1081108 GN=LEL_08976 PE=4 SV=1
MM1 pKa = 7.1QPAKK5 pKa = 10.22RR6 pKa = 11.84GRR8 pKa = 11.84SAKK11 pKa = 10.03RR12 pKa = 11.84GQPGKK17 pKa = 10.2RR18 pKa = 11.84GQSGKK23 pKa = 10.22RR24 pKa = 11.84GQPGKK29 pKa = 10.31RR30 pKa = 11.84GQPGKK35 pKa = 10.31RR36 pKa = 11.84GQPGKK41 pKa = 10.31RR42 pKa = 11.84GQPGKK47 pKa = 10.31RR48 pKa = 11.84GQPGKK53 pKa = 10.31RR54 pKa = 11.84GQPGKK59 pKa = 10.31RR60 pKa = 11.84GQPGKK65 pKa = 10.31RR66 pKa = 11.84GQPGKK71 pKa = 10.31RR72 pKa = 11.84GQPGKK77 pKa = 10.31RR78 pKa = 11.84GQPGKK83 pKa = 10.31RR84 pKa = 11.84GQPGKK89 pKa = 10.31RR90 pKa = 11.84GQPGKK95 pKa = 10.31RR96 pKa = 11.84GQPGKK101 pKa = 10.31RR102 pKa = 11.84GQPGKK107 pKa = 10.31RR108 pKa = 11.84GQPGKK113 pKa = 10.31RR114 pKa = 11.84GQPGKK119 pKa = 10.31RR120 pKa = 11.84GQPGKK125 pKa = 10.31RR126 pKa = 11.84GQPGKK131 pKa = 10.31RR132 pKa = 11.84GQPGKK137 pKa = 10.31RR138 pKa = 11.84GQPGKK143 pKa = 10.31RR144 pKa = 11.84GQPGKK149 pKa = 10.31RR150 pKa = 11.84GQPGKK155 pKa = 10.31RR156 pKa = 11.84GQPGKK161 pKa = 10.31RR162 pKa = 11.84GQPGKK167 pKa = 10.31RR168 pKa = 11.84GQPGKK173 pKa = 10.31RR174 pKa = 11.84GQPGKK179 pKa = 10.31RR180 pKa = 11.84GQPGKK185 pKa = 10.31RR186 pKa = 11.84GQPGKK191 pKa = 10.27RR192 pKa = 11.84GRR194 pKa = 11.84PGKK197 pKa = 10.16RR198 pKa = 11.84GQPGKK203 pKa = 10.31RR204 pKa = 11.84GQPGKK209 pKa = 10.32RR210 pKa = 11.84GQPTEE215 pKa = 4.15EE216 pKa = 4.15NSTGQLSRR224 pKa = 11.84RR225 pKa = 11.84QWAATVIRR233 pKa = 4.59

Molecular weight: 24.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11019

0

11019

5350041

49

6480

485.5

53.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.71 ± 0.025

1.241 ± 0.008

5.891 ± 0.017

5.876 ± 0.026

3.674 ± 0.013

7.101 ± 0.022

2.378 ± 0.01

4.632 ± 0.017

4.715 ± 0.021

8.826 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.296 ± 0.01

3.508 ± 0.013

5.81 ± 0.023

4.029 ± 0.016

6.053 ± 0.02

7.96 ± 0.026

5.899 ± 0.017

6.252 ± 0.016

1.461 ± 0.009

2.687 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski