Amino acid dipepetide frequency for Saccharomonospora phage PIS 136

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.527AlaAla: 15.527 ± 1.284
1.717AlaCys: 1.717 ± 0.263
6.87AlaAsp: 6.87 ± 0.613
6.659AlaGlu: 6.659 ± 0.624
2.664AlaPhe: 2.664 ± 0.314
8.797AlaGly: 8.797 ± 0.688
2.944AlaHis: 2.944 ± 0.315
3.926AlaIle: 3.926 ± 0.438
3.4AlaLys: 3.4 ± 0.406
9.288AlaLeu: 9.288 ± 0.642
2.524AlaMet: 2.524 ± 0.378
2.629AlaAsn: 2.629 ± 0.322
5.082AlaPro: 5.082 ± 0.44
2.629AlaGln: 2.629 ± 0.284
8.903AlaArg: 8.903 ± 0.835
6.73AlaSer: 6.73 ± 0.692
8.061AlaThr: 8.061 ± 0.592
8.868AlaVal: 8.868 ± 0.771
2.173AlaTrp: 2.173 ± 0.374
1.998AlaTyr: 1.998 ± 0.285
0.0AlaXaa: 0.0 ± 0.0
Cys
1.682CysAla: 1.682 ± 0.315
0.421CysCys: 0.421 ± 0.139
1.192CysAsp: 1.192 ± 0.323
0.876CysGlu: 0.876 ± 0.219
0.421CysPhe: 0.421 ± 0.173
1.893CysGly: 1.893 ± 0.337
0.21CysHis: 0.21 ± 0.079
0.245CysIle: 0.245 ± 0.094
0.315CysLys: 0.315 ± 0.112
1.016CysLeu: 1.016 ± 0.229
0.175CysMet: 0.175 ± 0.074
0.666CysAsn: 0.666 ± 0.16
0.841CysPro: 0.841 ± 0.183
0.491CysGln: 0.491 ± 0.167
1.472CysArg: 1.472 ± 0.209
1.227CysSer: 1.227 ± 0.306
0.701CysThr: 0.701 ± 0.15
1.157CysVal: 1.157 ± 0.168
0.28CysTrp: 0.28 ± 0.098
0.07CysTyr: 0.07 ± 0.043
0.0CysXaa: 0.0 ± 0.0
Asp
6.8AspAla: 6.8 ± 0.485
0.946AspCys: 0.946 ± 0.184
5.047AspAsp: 5.047 ± 0.75
3.996AspGlu: 3.996 ± 0.338
1.682AspPhe: 1.682 ± 0.238
6.519AspGly: 6.519 ± 0.659
1.788AspHis: 1.788 ± 0.248
2.559AspIle: 2.559 ± 0.351
1.227AspLys: 1.227 ± 0.227
5.257AspLeu: 5.257 ± 0.553
1.051AspMet: 1.051 ± 0.215
1.752AspAsn: 1.752 ± 0.265
4.591AspPro: 4.591 ± 0.429
2.453AspGln: 2.453 ± 0.369
5.292AspArg: 5.292 ± 0.677
3.295AspSer: 3.295 ± 0.299
4.031AspThr: 4.031 ± 0.373
4.767AspVal: 4.767 ± 0.644
1.437AspTrp: 1.437 ± 0.265
1.262AspTyr: 1.262 ± 0.237
0.0AspXaa: 0.0 ± 0.0
Glu
5.082GluAla: 5.082 ± 0.484
0.876GluCys: 0.876 ± 0.184
2.629GluAsp: 2.629 ± 0.301
2.734GluGlu: 2.734 ± 0.341
2.103GluPhe: 2.103 ± 0.281
4.031GluGly: 4.031 ± 0.473
2.243GluHis: 2.243 ± 0.308
2.033GluIle: 2.033 ± 0.328
1.682GluLys: 1.682 ± 0.28
6.134GluLeu: 6.134 ± 0.453
1.262GluMet: 1.262 ± 0.201
1.227GluAsn: 1.227 ± 0.291
4.451GluPro: 4.451 ± 0.478
3.225GluGln: 3.225 ± 0.342
5.222GluArg: 5.222 ± 0.498
2.559GluSer: 2.559 ± 0.38
3.61GluThr: 3.61 ± 0.487
4.662GluVal: 4.662 ± 0.406
1.472GluTrp: 1.472 ± 0.22
0.981GluTyr: 0.981 ± 0.202
0.0GluXaa: 0.0 ± 0.0
Phe
2.979PheAla: 2.979 ± 0.393
0.456PheCys: 0.456 ± 0.142
1.788PheAsp: 1.788 ± 0.286
1.472PheGlu: 1.472 ± 0.266
0.771PhePhe: 0.771 ± 0.179
1.858PheGly: 1.858 ± 0.286
0.666PheHis: 0.666 ± 0.181
0.561PheIle: 0.561 ± 0.151
0.631PheLys: 0.631 ± 0.171
1.893PheLeu: 1.893 ± 0.225
0.315PheMet: 0.315 ± 0.113
0.561PheAsn: 0.561 ± 0.138
1.051PhePro: 1.051 ± 0.179
0.806PheGln: 0.806 ± 0.152
1.752PheArg: 1.752 ± 0.231
1.542PheSer: 1.542 ± 0.258
1.858PheThr: 1.858 ± 0.261
1.647PheVal: 1.647 ± 0.258
0.421PheTrp: 0.421 ± 0.13
0.21PheTyr: 0.21 ± 0.077
0.0PheXaa: 0.0 ± 0.0
Gly
7.781GlyAla: 7.781 ± 0.573
1.262GlyCys: 1.262 ± 0.233
5.257GlyAsp: 5.257 ± 0.592
6.239GlyGlu: 6.239 ± 0.659
2.243GlyPhe: 2.243 ± 0.324
7.431GlyGly: 7.431 ± 0.891
2.348GlyHis: 2.348 ± 0.354
2.944GlyIle: 2.944 ± 0.284
2.348GlyLys: 2.348 ± 0.311
6.449GlyLeu: 6.449 ± 0.733
1.823GlyMet: 1.823 ± 0.265
1.858GlyAsn: 1.858 ± 0.299
3.225GlyPro: 3.225 ± 0.402
2.769GlyGln: 2.769 ± 0.383
6.344GlyArg: 6.344 ± 0.575
4.627GlySer: 4.627 ± 0.593
4.662GlyThr: 4.662 ± 0.578
7.536GlyVal: 7.536 ± 0.713
1.893GlyTrp: 1.893 ± 0.251
1.612GlyTyr: 1.612 ± 0.226
0.0GlyXaa: 0.0 ± 0.0
His
3.119HisAla: 3.119 ± 0.37
0.35HisCys: 0.35 ± 0.114
2.068HisAsp: 2.068 ± 0.325
1.472HisGlu: 1.472 ± 0.226
0.28HisPhe: 0.28 ± 0.098
2.068HisGly: 2.068 ± 0.307
1.262HisHis: 1.262 ± 0.225
0.946HisIle: 0.946 ± 0.182
0.526HisLys: 0.526 ± 0.115
2.839HisLeu: 2.839 ± 0.4
0.35HisMet: 0.35 ± 0.093
0.596HisAsn: 0.596 ± 0.124
2.348HisPro: 2.348 ± 0.339
1.402HisGln: 1.402 ± 0.323
2.734HisArg: 2.734 ± 0.482
1.016HisSer: 1.016 ± 0.168
1.928HisThr: 1.928 ± 0.26
2.313HisVal: 2.313 ± 0.361
0.421HisTrp: 0.421 ± 0.135
0.666HisTyr: 0.666 ± 0.165
0.0HisXaa: 0.0 ± 0.0
Ile
4.416IleAla: 4.416 ± 0.449
0.491IleCys: 0.491 ± 0.118
3.084IleAsp: 3.084 ± 0.41
2.769IleGlu: 2.769 ± 0.311
0.666IlePhe: 0.666 ± 0.167
2.699IleGly: 2.699 ± 0.348
1.051IleHis: 1.051 ± 0.186
0.841IleIle: 0.841 ± 0.232
0.701IleLys: 0.701 ± 0.168
2.278IleLeu: 2.278 ± 0.278
0.561IleMet: 0.561 ± 0.142
1.297IleAsn: 1.297 ± 0.257
2.348IlePro: 2.348 ± 0.275
0.981IleGln: 0.981 ± 0.176
2.734IleArg: 2.734 ± 0.29
2.278IleSer: 2.278 ± 0.321
2.383IleThr: 2.383 ± 0.298
2.769IleVal: 2.769 ± 0.312
0.175IleTrp: 0.175 ± 0.067
0.386IleTyr: 0.386 ± 0.117
0.0IleXaa: 0.0 ± 0.0
Lys
3.47LysAla: 3.47 ± 0.433
0.175LysCys: 0.175 ± 0.077
1.227LysAsp: 1.227 ± 0.198
1.297LysGlu: 1.297 ± 0.297
0.315LysPhe: 0.315 ± 0.104
2.068LysGly: 2.068 ± 0.319
0.631LysHis: 0.631 ± 0.15
0.911LysIle: 0.911 ± 0.2
1.367LysLys: 1.367 ± 0.338
2.313LysLeu: 2.313 ± 0.339
0.666LysMet: 0.666 ± 0.137
0.631LysAsn: 0.631 ± 0.139
1.823LysPro: 1.823 ± 0.285
1.402LysGln: 1.402 ± 0.266
2.138LysArg: 2.138 ± 0.316
1.157LysSer: 1.157 ± 0.191
1.577LysThr: 1.577 ± 0.278
1.472LysVal: 1.472 ± 0.297
0.386LysTrp: 0.386 ± 0.108
0.526LysTyr: 0.526 ± 0.147
0.0LysXaa: 0.0 ± 0.0
Leu
9.849LeuAla: 9.849 ± 0.724
1.367LeuCys: 1.367 ± 0.302
6.134LeuAsp: 6.134 ± 0.462
4.101LeuGlu: 4.101 ± 0.404
1.823LeuPhe: 1.823 ± 0.223
6.589LeuGly: 6.589 ± 0.63
2.208LeuHis: 2.208 ± 0.471
2.734LeuIle: 2.734 ± 0.34
1.928LeuLys: 1.928 ± 0.377
7.36LeuLeu: 7.36 ± 0.839
1.577LeuMet: 1.577 ± 0.215
1.963LeuAsn: 1.963 ± 0.222
5.573LeuPro: 5.573 ± 0.417
2.033LeuGln: 2.033 ± 0.444
8.692LeuArg: 8.692 ± 0.87
4.872LeuSer: 4.872 ± 0.357
5.047LeuThr: 5.047 ± 0.413
7.08LeuVal: 7.08 ± 0.527
1.437LeuTrp: 1.437 ± 0.231
1.262LeuTyr: 1.262 ± 0.219
0.0LeuXaa: 0.0 ± 0.0
Met
1.963MetAla: 1.963 ± 0.314
0.421MetCys: 0.421 ± 0.144
1.227MetAsp: 1.227 ± 0.221
0.876MetGlu: 0.876 ± 0.182
0.421MetPhe: 0.421 ± 0.117
1.402MetGly: 1.402 ± 0.266
0.421MetHis: 0.421 ± 0.102
0.666MetIle: 0.666 ± 0.166
0.526MetLys: 0.526 ± 0.129
2.348MetLeu: 2.348 ± 0.321
0.386MetMet: 0.386 ± 0.102
0.701MetAsn: 0.701 ± 0.16
1.647MetPro: 1.647 ± 0.242
0.736MetGln: 0.736 ± 0.217
1.612MetArg: 1.612 ± 0.242
1.507MetSer: 1.507 ± 0.236
2.383MetThr: 2.383 ± 0.302
1.262MetVal: 1.262 ± 0.195
0.35MetTrp: 0.35 ± 0.098
0.245MetTyr: 0.245 ± 0.089
0.0MetXaa: 0.0 ± 0.0
Asn
2.944AsnAla: 2.944 ± 0.328
0.28AsnCys: 0.28 ± 0.118
1.577AsnAsp: 1.577 ± 0.227
1.016AsnGlu: 1.016 ± 0.196
0.526AsnPhe: 0.526 ± 0.141
2.524AsnGly: 2.524 ± 0.323
1.192AsnHis: 1.192 ± 0.281
1.051AsnIle: 1.051 ± 0.17
0.771AsnLys: 0.771 ± 0.18
2.243AsnLeu: 2.243 ± 0.272
0.386AsnMet: 0.386 ± 0.128
0.771AsnAsn: 0.771 ± 0.116
2.418AsnPro: 2.418 ± 0.43
0.771AsnGln: 0.771 ± 0.171
2.138AsnArg: 2.138 ± 0.299
1.402AsnSer: 1.402 ± 0.228
2.208AsnThr: 2.208 ± 0.249
1.682AsnVal: 1.682 ± 0.303
0.35AsnTrp: 0.35 ± 0.107
0.736AsnTyr: 0.736 ± 0.15
0.0AsnXaa: 0.0 ± 0.0
Pro
6.659ProAla: 6.659 ± 0.51
1.297ProCys: 1.297 ± 0.244
3.19ProAsp: 3.19 ± 0.373
4.311ProGlu: 4.311 ± 0.554
0.946ProPhe: 0.946 ± 0.19
4.942ProGly: 4.942 ± 0.43
1.367ProHis: 1.367 ± 0.268
2.138ProIle: 2.138 ± 0.277
1.507ProLys: 1.507 ± 0.278
4.767ProLeu: 4.767 ± 0.479
1.297ProMet: 1.297 ± 0.202
1.472ProAsn: 1.472 ± 0.275
3.715ProPro: 3.715 ± 0.44
2.559ProGln: 2.559 ± 0.309
4.872ProArg: 4.872 ± 0.505
3.54ProSer: 3.54 ± 0.365
4.276ProThr: 4.276 ± 0.452
5.398ProVal: 5.398 ± 0.559
0.981ProTrp: 0.981 ± 0.183
1.227ProTyr: 1.227 ± 0.264
0.0ProXaa: 0.0 ± 0.0
Gln
4.451GlnAla: 4.451 ± 0.431
0.631GlnCys: 0.631 ± 0.141
1.682GlnAsp: 1.682 ± 0.255
1.437GlnGlu: 1.437 ± 0.245
0.736GlnPhe: 0.736 ± 0.192
2.138GlnGly: 2.138 ± 0.283
1.087GlnHis: 1.087 ± 0.267
1.367GlnIle: 1.367 ± 0.22
0.736GlnLys: 0.736 ± 0.191
3.014GlnLeu: 3.014 ± 0.276
0.876GlnMet: 0.876 ± 0.176
0.596GlnAsn: 0.596 ± 0.141
2.524GlnPro: 2.524 ± 0.399
2.559GlnGln: 2.559 ± 0.308
4.276GlnArg: 4.276 ± 0.768
1.402GlnSer: 1.402 ± 0.24
1.788GlnThr: 1.788 ± 0.246
3.154GlnVal: 3.154 ± 0.342
0.666GlnTrp: 0.666 ± 0.146
0.876GlnTyr: 0.876 ± 0.151
0.0GlnXaa: 0.0 ± 0.0
Arg
8.657ArgAla: 8.657 ± 0.754
1.051ArgCys: 1.051 ± 0.223
6.169ArgAsp: 6.169 ± 0.552
5.187ArgGlu: 5.187 ± 0.518
2.313ArgPhe: 2.313 ± 0.295
5.608ArgGly: 5.608 ± 0.672
3.26ArgHis: 3.26 ± 0.537
3.014ArgIle: 3.014 ± 0.359
3.084ArgLys: 3.084 ± 0.331
6.694ArgLeu: 6.694 ± 0.829
2.909ArgMet: 2.909 ± 0.35
3.26ArgAsn: 3.26 ± 0.47
5.117ArgPro: 5.117 ± 0.69
3.435ArgGln: 3.435 ± 0.463
8.797ArgArg: 8.797 ± 0.964
4.451ArgSer: 4.451 ± 0.46
4.732ArgThr: 4.732 ± 0.503
7.36ArgVal: 7.36 ± 0.707
1.542ArgTrp: 1.542 ± 0.217
1.612ArgTyr: 1.612 ± 0.232
0.0ArgXaa: 0.0 ± 0.0
Ser
6.87SerAla: 6.87 ± 0.668
0.911SerCys: 0.911 ± 0.233
3.54SerAsp: 3.54 ± 0.344
3.014SerGlu: 3.014 ± 0.296
1.332SerPhe: 1.332 ± 0.215
5.958SerGly: 5.958 ± 0.538
0.736SerHis: 0.736 ± 0.144
1.963SerIle: 1.963 ± 0.253
1.542SerLys: 1.542 ± 0.288
4.521SerLeu: 4.521 ± 0.445
1.297SerMet: 1.297 ± 0.198
1.542SerAsn: 1.542 ± 0.215
3.47SerPro: 3.47 ± 0.451
1.682SerGln: 1.682 ± 0.301
4.767SerArg: 4.767 ± 0.491
3.961SerSer: 3.961 ± 0.621
3.785SerThr: 3.785 ± 0.487
4.241SerVal: 4.241 ± 0.45
0.701SerTrp: 0.701 ± 0.18
0.771SerTyr: 0.771 ± 0.173
0.0SerXaa: 0.0 ± 0.0
Thr
7.01ThrAla: 7.01 ± 0.68
0.806ThrCys: 0.806 ± 0.18
4.521ThrAsp: 4.521 ± 0.509
3.435ThrGlu: 3.435 ± 0.385
1.367ThrPhe: 1.367 ± 0.236
6.274ThrGly: 6.274 ± 0.595
1.542ThrHis: 1.542 ± 0.261
2.979ThrIle: 2.979 ± 0.319
1.192ThrLys: 1.192 ± 0.19
5.538ThrLeu: 5.538 ± 0.448
1.227ThrMet: 1.227 ± 0.216
2.313ThrAsn: 2.313 ± 0.313
4.486ThrPro: 4.486 ± 0.52
1.682ThrGln: 1.682 ± 0.294
6.029ThrArg: 6.029 ± 0.498
3.961ThrSer: 3.961 ± 0.414
5.047ThrThr: 5.047 ± 0.561
5.222ThrVal: 5.222 ± 0.489
1.016ThrTrp: 1.016 ± 0.256
1.122ThrTyr: 1.122 ± 0.211
0.0ThrXaa: 0.0 ± 0.0
Val
9.043ValAla: 9.043 ± 0.693
1.122ValCys: 1.122 ± 0.202
6.169ValAsp: 6.169 ± 0.557
5.047ValGlu: 5.047 ± 0.409
1.682ValPhe: 1.682 ± 0.201
5.608ValGly: 5.608 ± 0.585
2.769ValHis: 2.769 ± 0.444
2.699ValIle: 2.699 ± 0.332
1.472ValLys: 1.472 ± 0.259
6.274ValLeu: 6.274 ± 0.768
1.682ValMet: 1.682 ± 0.299
2.173ValAsn: 2.173 ± 0.279
4.276ValPro: 4.276 ± 0.461
2.944ValGln: 2.944 ± 0.374
6.975ValArg: 6.975 ± 0.799
4.732ValSer: 4.732 ± 0.375
5.923ValThr: 5.923 ± 0.537
7.431ValVal: 7.431 ± 0.733
1.262ValTrp: 1.262 ± 0.261
1.577ValTyr: 1.577 ± 0.229
0.0ValXaa: 0.0 ± 0.0
Trp
1.577TrpAla: 1.577 ± 0.266
0.596TrpCys: 0.596 ± 0.178
1.157TrpAsp: 1.157 ± 0.189
1.051TrpGlu: 1.051 ± 0.206
0.526TrpPhe: 0.526 ± 0.128
0.981TrpGly: 0.981 ± 0.163
0.561TrpHis: 0.561 ± 0.115
0.736TrpIle: 0.736 ± 0.166
0.315TrpLys: 0.315 ± 0.107
1.823TrpLeu: 1.823 ± 0.317
0.491TrpMet: 0.491 ± 0.117
0.491TrpAsn: 0.491 ± 0.144
0.841TrpPro: 0.841 ± 0.183
0.736TrpGln: 0.736 ± 0.162
1.542TrpArg: 1.542 ± 0.25
1.297TrpSer: 1.297 ± 0.241
0.876TrpThr: 0.876 ± 0.183
1.332TrpVal: 1.332 ± 0.216
0.35TrpTrp: 0.35 ± 0.12
0.421TrpTyr: 0.421 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.507TyrAla: 1.507 ± 0.263
0.175TyrCys: 0.175 ± 0.087
1.227TyrAsp: 1.227 ± 0.222
1.227TyrGlu: 1.227 ± 0.264
0.421TyrPhe: 0.421 ± 0.13
1.262TyrGly: 1.262 ± 0.206
0.421TyrHis: 0.421 ± 0.112
0.596TyrIle: 0.596 ± 0.13
0.28TyrLys: 0.28 ± 0.114
1.577TyrLeu: 1.577 ± 0.19
0.21TyrMet: 0.21 ± 0.071
0.491TyrAsn: 0.491 ± 0.131
0.631TyrPro: 0.631 ± 0.159
0.736TyrGln: 0.736 ± 0.151
1.963TyrArg: 1.963 ± 0.25
1.051TyrSer: 1.051 ± 0.21
1.893TyrThr: 1.893 ± 0.303
1.507TyrVal: 1.507 ± 0.262
0.386TyrTrp: 0.386 ± 0.126
0.315TyrTyr: 0.315 ± 0.14
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 132 proteins (28532 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski