Amino acid dipepetide frequency for Microbacterium sp. C448

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.347AlaAla: 19.347 ± 0.2	0.693AlaCys: 0.693 ± 0.031	8.129AlaAsp: 8.129 ± 0.098	8.188AlaGlu: 8.188 ± 0.099	3.902AlaPhe: 3.902 ± 0.066	11.475AlaGly: 11.475 ± 0.11	2.478AlaHis: 2.478 ± 0.055	6.328AlaIle: 6.328 ± 0.097	2.471AlaLys: 2.471 ± 0.059	14.124AlaLeu: 14.124 ± 0.148	2.587AlaMet: 2.587 ± 0.049	2.297AlaAsn: 2.297 ± 0.05	6.487AlaPro: 6.487 ± 0.108	3.664AlaGln: 3.664 ± 0.068	9.75AlaArg: 9.75 ± 0.114	7.93AlaSer: 7.93 ± 0.093	7.271AlaThr: 7.271 ± 0.097	10.917AlaVal: 10.917 ± 0.129	1.971AlaTrp: 1.971 ± 0.044	2.476AlaTyr: 2.476 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.64CysAla: 0.64 ± 0.028	0.046CysCys: 0.046 ± 0.007	0.32CysAsp: 0.32 ± 0.018	0.269CysGlu: 0.269 ± 0.019	0.171CysPhe: 0.171 ± 0.013	0.512CysGly: 0.512 ± 0.025	0.129CysHis: 0.129 ± 0.013	0.209CysIle: 0.209 ± 0.017	0.065CysLys: 0.065 ± 0.008	0.423CysLeu: 0.423 ± 0.023	0.083CysMet: 0.083 ± 0.009	0.1CysAsn: 0.1 ± 0.011	0.303CysPro: 0.303 ± 0.019	0.118CysGln: 0.118 ± 0.012	0.359CysArg: 0.359 ± 0.022	0.332CysSer: 0.332 ± 0.018	0.293CysThr: 0.293 ± 0.018	0.404CysVal: 0.404 ± 0.02	0.067CysTrp: 0.067 ± 0.009	0.092CysTyr: 0.092 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	9.075AspAla: 9.075 ± 0.11	0.23AspCys: 0.23 ± 0.014	4.318AspAsp: 4.318 ± 0.07	4.628AspGlu: 4.628 ± 0.074	1.784AspPhe: 1.784 ± 0.046	5.836AspGly: 5.836 ± 0.096	1.201AspHis: 1.201 ± 0.037	2.784AspIle: 2.784 ± 0.052	0.978AspLys: 0.978 ± 0.038	6.131AspLeu: 6.131 ± 0.084	0.964AspMet: 0.964 ± 0.03	1.001AspAsn: 1.001 ± 0.032	4.268AspPro: 4.268 ± 0.075	1.491AspGln: 1.491 ± 0.042	4.556AspArg: 4.556 ± 0.077	2.838AspSer: 2.838 ± 0.059	3.022AspThr: 3.022 ± 0.061	5.776AspVal: 5.776 ± 0.088	0.929AspTrp: 0.929 ± 0.032	1.3AspTyr: 1.3 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	7.282GluAla: 7.282 ± 0.086	0.224GluCys: 0.224 ± 0.016	3.024GluAsp: 3.024 ± 0.06	2.821GluGlu: 2.821 ± 0.076	1.746GluPhe: 1.746 ± 0.043	4.359GluGly: 4.359 ± 0.071	1.516GluHis: 1.516 ± 0.047	2.878GluIle: 2.878 ± 0.062	1.323GluLys: 1.323 ± 0.042	6.668GluLeu: 6.668 ± 0.096	0.945GluMet: 0.945 ± 0.032	1.299GluAsn: 1.299 ± 0.039	3.063GluPro: 3.063 ± 0.07	2.083GluGln: 2.083 ± 0.042	5.502GluArg: 5.502 ± 0.085	3.15GluSer: 3.15 ± 0.057	3.135GluThr: 3.135 ± 0.065	4.526GluVal: 4.526 ± 0.075	0.949GluTrp: 0.949 ± 0.033	1.182GluTyr: 1.182 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	4.438PheAla: 4.438 ± 0.074	0.151PheCys: 0.151 ± 0.014	2.423PheAsp: 2.423 ± 0.05	1.74PheGlu: 1.74 ± 0.043	1.101PhePhe: 1.101 ± 0.037	3.339PheGly: 3.339 ± 0.062	0.551PheHis: 0.551 ± 0.024	1.273PheIle: 1.273 ± 0.041	0.376PheLys: 0.376 ± 0.022	2.795PheLeu: 2.795 ± 0.056	0.43PheMet: 0.43 ± 0.021	0.602PheAsn: 0.602 ± 0.023	1.429PhePro: 1.429 ± 0.041	0.762PheGln: 0.762 ± 0.031	1.871PheArg: 1.871 ± 0.044	1.737PheSer: 1.737 ± 0.037	1.985PheThr: 1.985 ± 0.054	2.895PheVal: 2.895 ± 0.055	0.486PheTrp: 0.486 ± 0.024	0.633PheTyr: 0.633 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	10.49GlyAla: 10.49 ± 0.117	0.608GlyCys: 0.608 ± 0.027	4.992GlyAsp: 4.992 ± 0.073	4.992GlyGlu: 4.992 ± 0.07	3.224GlyPhe: 3.224 ± 0.055	7.409GlyGly: 7.409 ± 0.105	1.848GlyHis: 1.848 ± 0.047	4.91GlyIle: 4.91 ± 0.067	2.055GlyLys: 2.055 ± 0.057	8.63GlyLeu: 8.63 ± 0.095	2.116GlyMet: 2.116 ± 0.05	1.648GlyAsn: 1.648 ± 0.042	3.847GlyPro: 3.847 ± 0.063	2.361GlyGln: 2.361 ± 0.049	6.437GlyArg: 6.437 ± 0.087	5.307GlySer: 5.307 ± 0.077	5.406GlyThr: 5.406 ± 0.079	7.838GlyVal: 7.838 ± 0.094	1.644GlyTrp: 1.644 ± 0.047	2.065GlyTyr: 2.065 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	2.375HisAla: 2.375 ± 0.058	0.113HisCys: 0.113 ± 0.012	1.375HisAsp: 1.375 ± 0.04	1.18HisGlu: 1.18 ± 0.04	0.532HisPhe: 0.532 ± 0.022	1.961HisGly: 1.961 ± 0.052	0.572HisHis: 0.572 ± 0.025	0.761HisIle: 0.761 ± 0.026	0.328HisLys: 0.328 ± 0.019	1.96HisLeu: 1.96 ± 0.048	0.325HisMet: 0.325 ± 0.017	0.363HisAsn: 0.363 ± 0.017	1.572HisPro: 1.572 ± 0.046	0.501HisGln: 0.501 ± 0.021	1.604HisArg: 1.604 ± 0.043	0.971HisSer: 0.971 ± 0.029	1.051HisThr: 1.051 ± 0.035	1.713HisVal: 1.713 ± 0.042	0.264HisTrp: 0.264 ± 0.018	0.412HisTyr: 0.412 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.306IleAla: 7.306 ± 0.097	0.253IleCys: 0.253 ± 0.019	3.744IleAsp: 3.744 ± 0.064	3.175IleGlu: 3.175 ± 0.061	1.249IlePhe: 1.249 ± 0.042	4.737IleGly: 4.737 ± 0.076	0.749IleHis: 0.749 ± 0.029	1.952IleIle: 1.952 ± 0.059	0.774IleLys: 0.774 ± 0.034	3.967IleLeu: 3.967 ± 0.074	0.68IleMet: 0.68 ± 0.025	0.915IleAsn: 0.915 ± 0.034	2.511IlePro: 2.511 ± 0.047	1.087IleGln: 1.087 ± 0.031	2.958IleArg: 2.958 ± 0.057	2.443IleSer: 2.443 ± 0.058	2.862IleThr: 2.862 ± 0.06	5.158IleVal: 5.158 ± 0.08	0.542IleTrp: 0.542 ± 0.025	0.814IleTyr: 0.814 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	2.307LysAla: 2.307 ± 0.055	0.075LysCys: 0.075 ± 0.01	1.021LysAsp: 1.021 ± 0.038	0.803LysGlu: 0.803 ± 0.028	0.47LysPhe: 0.47 ± 0.022	1.441LysGly: 1.441 ± 0.044	0.473LysHis: 0.473 ± 0.02	0.94LysIle: 0.94 ± 0.038	0.766LysLys: 0.766 ± 0.036	1.742LysLeu: 1.742 ± 0.05	0.362LysMet: 0.362 ± 0.021	0.476LysAsn: 0.476 ± 0.027	1.132LysPro: 1.132 ± 0.041	0.619LysGln: 0.619 ± 0.03	1.653LysArg: 1.653 ± 0.044	1.103LysSer: 1.103 ± 0.036	1.271LysThr: 1.271 ± 0.038	1.545LysVal: 1.545 ± 0.042	0.252LysTrp: 0.252 ± 0.016	0.423LysTyr: 0.423 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	14.452LeuAla: 14.452 ± 0.154	0.5LeuCys: 0.5 ± 0.022	6.771LeuAsp: 6.771 ± 0.086	5.44LeuGlu: 5.44 ± 0.082	2.907LeuPhe: 2.907 ± 0.06	8.966LeuGly: 8.966 ± 0.116	1.757LeuHis: 1.757 ± 0.051	4.8LeuIle: 4.8 ± 0.089	1.603LeuLys: 1.603 ± 0.048	9.737LeuLeu: 9.737 ± 0.128	1.638LeuMet: 1.638 ± 0.045	1.742LeuAsn: 1.742 ± 0.04	5.522LeuPro: 5.522 ± 0.087	2.45LeuGln: 2.45 ± 0.05	7.83LeuArg: 7.83 ± 0.086	5.846LeuSer: 5.846 ± 0.083	6.179LeuThr: 6.179 ± 0.085	9.081LeuVal: 9.081 ± 0.123	1.348LeuTrp: 1.348 ± 0.044	1.621LeuTyr: 1.621 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	2.065MetAla: 2.065 ± 0.045	0.086MetCys: 0.086 ± 0.01	0.874MetAsp: 0.874 ± 0.031	0.636MetGlu: 0.636 ± 0.029	0.503MetPhe: 0.503 ± 0.023	1.325MetGly: 1.325 ± 0.04	0.361MetHis: 0.361 ± 0.021	0.904MetIle: 0.904 ± 0.033	0.454MetLys: 0.454 ± 0.023	1.921MetLeu: 1.921 ± 0.046	0.32MetMet: 0.32 ± 0.017	0.535MetAsn: 0.535 ± 0.022	1.278MetPro: 1.278 ± 0.04	0.528MetGln: 0.528 ± 0.021	1.551MetArg: 1.551 ± 0.038	1.654MetSer: 1.654 ± 0.045	1.681MetThr: 1.681 ± 0.042	1.305MetVal: 1.305 ± 0.037	0.204MetTrp: 0.204 ± 0.017	0.279MetTyr: 0.279 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.506AsnAla: 2.506 ± 0.053	0.098AsnCys: 0.098 ± 0.011	1.156AsnAsp: 1.156 ± 0.036	1.06AsnGlu: 1.06 ± 0.035	0.614AsnPhe: 0.614 ± 0.028	1.814AsnGly: 1.814 ± 0.043	0.366AsnHis: 0.366 ± 0.018	0.864AsnIle: 0.864 ± 0.029	0.394AsnLys: 0.394 ± 0.022	1.942AsnLeu: 1.942 ± 0.044	0.347AsnMet: 0.347 ± 0.018	0.447AsnAsn: 0.447 ± 0.023	1.608AsnPro: 1.608 ± 0.034	0.521AsnGln: 0.521 ± 0.025	1.314AsnArg: 1.314 ± 0.044	0.947AsnSer: 0.947 ± 0.036	1.127AsnThr: 1.127 ± 0.036	1.697AsnVal: 1.697 ± 0.039	0.274AsnTrp: 0.274 ± 0.017	0.451AsnTyr: 0.451 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	6.901ProAla: 6.901 ± 0.109	0.187ProCys: 0.187 ± 0.013	4.096ProAsp: 4.096 ± 0.076	4.043ProGlu: 4.043 ± 0.065	1.778ProPhe: 1.778 ± 0.047	5.024ProGly: 5.024 ± 0.079	1.192ProHis: 1.192 ± 0.035	2.294ProIle: 2.294 ± 0.049	0.997ProLys: 0.997 ± 0.035	5.051ProLeu: 5.051 ± 0.077	0.904ProMet: 0.904 ± 0.029	1.038ProAsn: 1.038 ± 0.032	2.589ProPro: 2.589 ± 0.074	1.436ProGln: 1.436 ± 0.035	3.877ProArg: 3.877 ± 0.064	3.234ProSer: 3.234 ± 0.066	3.371ProThr: 3.371 ± 0.064	4.813ProVal: 4.813 ± 0.084	0.934ProTrp: 0.934 ± 0.033	1.016ProTyr: 1.016 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	3.328GlnAla: 3.328 ± 0.069	0.104GlnCys: 0.104 ± 0.012	1.311GlnAsp: 1.311 ± 0.036	1.168GlnGlu: 1.168 ± 0.035	0.794GlnPhe: 0.794 ± 0.024	2.032GlnGly: 2.032 ± 0.048	0.603GlnHis: 0.603 ± 0.025	1.452GlnIle: 1.452 ± 0.039	0.659GlnLys: 0.659 ± 0.028	3.025GlnLeu: 3.025 ± 0.061	0.535GlnMet: 0.535 ± 0.023	0.658GlnAsn: 0.658 ± 0.035	1.5GlnPro: 1.5 ± 0.044	1.062GlnGln: 1.062 ± 0.035	2.415GlnArg: 2.415 ± 0.054	1.458GlnSer: 1.458 ± 0.038	1.588GlnThr: 1.588 ± 0.038	2.223GlnVal: 2.223 ± 0.047	0.411GlnTrp: 0.411 ± 0.02	0.543GlnTyr: 0.543 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	9.547ArgAla: 9.547 ± 0.115	0.306ArgCys: 0.306 ± 0.021	4.65ArgAsp: 4.65 ± 0.071	4.717ArgGlu: 4.717 ± 0.079	2.366ArgPhe: 2.366 ± 0.048	5.828ArgGly: 5.828 ± 0.078	1.639ArgHis: 1.639 ± 0.043	3.73ArgIle: 3.73 ± 0.06	1.44ArgLys: 1.44 ± 0.038	7.579ArgLeu: 7.579 ± 0.098	1.873ArgMet: 1.873 ± 0.045	1.461ArgAsn: 1.461 ± 0.035	3.895ArgPro: 3.895 ± 0.077	2.063ArgGln: 2.063 ± 0.044	7.052ArgArg: 7.052 ± 0.099	4.353ArgSer: 4.353 ± 0.08	4.441ArgThr: 4.441 ± 0.068	6.468ArgVal: 6.468 ± 0.089	1.195ArgTrp: 1.195 ± 0.041	1.648ArgTyr: 1.648 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	7.396SerAla: 7.396 ± 0.079	0.299SerCys: 0.299 ± 0.023	3.448SerAsp: 3.448 ± 0.061	2.952SerGlu: 2.952 ± 0.061	1.899SerPhe: 1.899 ± 0.044	5.901SerGly: 5.901 ± 0.096	1.064SerHis: 1.064 ± 0.037	2.691SerIle: 2.691 ± 0.053	1.056SerLys: 1.056 ± 0.039	5.433SerLeu: 5.433 ± 0.073	1.188SerMet: 1.188 ± 0.036	1.149SerAsn: 1.149 ± 0.034	3.231SerPro: 3.231 ± 0.062	1.368SerGln: 1.368 ± 0.042	4.106SerArg: 4.106 ± 0.068	3.563SerSer: 3.563 ± 0.063	3.669SerThr: 3.669 ± 0.066	5.019SerVal: 5.019 ± 0.077	0.94SerTrp: 0.94 ± 0.028	1.131SerTyr: 1.131 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	7.24ThrAla: 7.24 ± 0.095	0.273ThrCys: 0.273 ± 0.017	3.73ThrAsp: 3.73 ± 0.069	3.004ThrGlu: 3.004 ± 0.059	1.918ThrPhe: 1.918 ± 0.046	5.648ThrGly: 5.648 ± 0.082	1.186ThrHis: 1.186 ± 0.037	3.057ThrIle: 3.057 ± 0.054	1.131ThrLys: 1.131 ± 0.035	6.111ThrLeu: 6.111 ± 0.086	1.088ThrMet: 1.088 ± 0.036	1.178ThrAsn: 1.178 ± 0.039	3.917ThrPro: 3.917 ± 0.077	1.477ThrGln: 1.477 ± 0.041	4.034ThrArg: 4.034 ± 0.069	3.476ThrSer: 3.476 ± 0.057	3.673ThrThr: 3.673 ± 0.075	5.41ThrVal: 5.41 ± 0.079	0.862ThrTrp: 0.862 ± 0.033	1.173ThrTyr: 1.173 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	11.715ValAla: 11.715 ± 0.122	0.474ValCys: 0.474 ± 0.026	5.654ValAsp: 5.654 ± 0.077	5.029ValGlu: 5.029 ± 0.074	2.789ValPhe: 2.789 ± 0.058	7.243ValGly: 7.243 ± 0.111	1.658ValHis: 1.658 ± 0.044	4.533ValIle: 4.533 ± 0.071	1.415ValLys: 1.415 ± 0.042	9.093ValLeu: 9.093 ± 0.117	1.458ValMet: 1.458 ± 0.043	1.772ValAsn: 1.772 ± 0.04	4.779ValPro: 4.779 ± 0.075	2.15ValGln: 2.15 ± 0.047	6.464ValArg: 6.464 ± 0.084	5.149ValSer: 5.149 ± 0.069	5.542ValThr: 5.542 ± 0.082	8.717ValVal: 8.717 ± 0.134	1.18ValTrp: 1.18 ± 0.035	1.546ValTyr: 1.546 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.628TrpAla: 1.628 ± 0.042	0.111TrpCys: 0.111 ± 0.011	0.787TrpAsp: 0.787 ± 0.032	0.663TrpGlu: 0.663 ± 0.029	0.609TrpPhe: 0.609 ± 0.028	1.095TrpGly: 1.095 ± 0.034	0.317TrpHis: 0.317 ± 0.018	0.803TrpIle: 0.803 ± 0.033	0.27TrpLys: 0.27 ± 0.015	1.747TrpLeu: 1.747 ± 0.05	0.353TrpMet: 0.353 ± 0.019	0.411TrpAsn: 0.411 ± 0.021	0.739TrpPro: 0.739 ± 0.028	0.571TrpGln: 0.571 ± 0.024	1.293TrpArg: 1.293 ± 0.037	0.931TrpSer: 0.931 ± 0.033	0.911TrpThr: 0.911 ± 0.03	1.22TrpVal: 1.22 ± 0.036	0.379TrpTrp: 0.379 ± 0.021	0.297TrpTyr: 0.297 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.448TyrAla: 2.448 ± 0.055	0.102TyrCys: 0.102 ± 0.01	1.286TyrAsp: 1.286 ± 0.038	1.169TyrGlu: 1.169 ± 0.037	0.662TyrPhe: 0.662 ± 0.028	1.884TyrGly: 1.884 ± 0.045	0.275TyrHis: 0.275 ± 0.018	0.713TyrIle: 0.713 ± 0.029	0.317TyrLys: 0.317 ± 0.017	2.112TyrLeu: 2.112 ± 0.053	0.265TyrMet: 0.265 ± 0.017	0.455TyrAsn: 0.455 ± 0.023	1.103TyrPro: 1.103 ± 0.036	0.558TyrGln: 0.558 ± 0.024	1.629TyrArg: 1.629 ± 0.042	1.082TyrSer: 1.082 ± 0.029	1.105TyrThr: 1.105 ± 0.034	1.632TyrVal: 1.632 ± 0.045	0.304TyrTrp: 0.304 ± 0.018	0.483TyrTyr: 0.483 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3165 proteins (972437 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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