Amino acid dipepetide frequency for Pedobacter sp. RP-1-13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.555AlaAla: 5.555 ± 0.072	0.594AlaCys: 0.594 ± 0.02	4.152AlaAsp: 4.152 ± 0.049	4.287AlaGlu: 4.287 ± 0.053	3.427AlaPhe: 3.427 ± 0.053	5.067AlaGly: 5.067 ± 0.07	1.102AlaHis: 1.102 ± 0.024	5.733AlaIle: 5.733 ± 0.065	5.542AlaLys: 5.542 ± 0.063	6.586AlaLeu: 6.586 ± 0.066	1.628AlaMet: 1.628 ± 0.037	4.471AlaAsn: 4.471 ± 0.057	2.214AlaPro: 2.214 ± 0.045	2.905AlaGln: 2.905 ± 0.043	2.158AlaArg: 2.158 ± 0.034	4.442AlaSer: 4.442 ± 0.067	4.346AlaThr: 4.346 ± 0.077	4.473AlaVal: 4.473 ± 0.061	0.746AlaTrp: 0.746 ± 0.023	2.967AlaTyr: 2.967 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.498CysAla: 0.498 ± 0.021	0.128CysCys: 0.128 ± 0.009	0.379CysAsp: 0.379 ± 0.015	0.389CysGlu: 0.389 ± 0.015	0.421CysPhe: 0.421 ± 0.017	0.55CysGly: 0.55 ± 0.024	0.16CysHis: 0.16 ± 0.01	0.579CysIle: 0.579 ± 0.019	0.53CysLys: 0.53 ± 0.016	0.748CysLeu: 0.748 ± 0.023	0.15CysMet: 0.15 ± 0.01	0.4CysAsn: 0.4 ± 0.015	0.28CysPro: 0.28 ± 0.013	0.201CysGln: 0.201 ± 0.011	0.239CysArg: 0.239 ± 0.012	0.498CysSer: 0.498 ± 0.02	0.402CysThr: 0.402 ± 0.015	0.419CysVal: 0.419 ± 0.016	0.081CysTrp: 0.081 ± 0.007	0.284CysTyr: 0.284 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.785AspAla: 3.785 ± 0.052	0.404AspCys: 0.404 ± 0.017	2.527AspAsp: 2.527 ± 0.041	3.483AspGlu: 3.483 ± 0.05	3.233AspPhe: 3.233 ± 0.044	3.935AspGly: 3.935 ± 0.054	0.855AspHis: 0.855 ± 0.021	3.845AspIle: 3.845 ± 0.052	4.216AspLys: 4.216 ± 0.065	5.129AspLeu: 5.129 ± 0.048	1.075AspMet: 1.075 ± 0.028	2.765AspAsn: 2.765 ± 0.037	1.929AspPro: 1.929 ± 0.039	1.773AspGln: 1.773 ± 0.035	1.901AspArg: 1.901 ± 0.034	2.789AspSer: 2.789 ± 0.047	2.447AspThr: 2.447 ± 0.041	3.443AspVal: 3.443 ± 0.042	0.814AspTrp: 0.814 ± 0.024	2.552AspTyr: 2.552 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	3.865GluAla: 3.865 ± 0.057	0.291GluCys: 0.291 ± 0.012	2.731GluAsp: 2.731 ± 0.044	3.578GluGlu: 3.578 ± 0.063	2.482GluPhe: 2.482 ± 0.046	3.223GluGly: 3.223 ± 0.039	0.931GluHis: 0.931 ± 0.023	4.651GluIle: 4.651 ± 0.054	5.118GluLys: 5.118 ± 0.059	5.719GluLeu: 5.719 ± 0.076	1.464GluMet: 1.464 ± 0.033	3.756GluAsn: 3.756 ± 0.05	1.601GluPro: 1.601 ± 0.029	2.287GluGln: 2.287 ± 0.044	2.273GluArg: 2.273 ± 0.041	2.729GluSer: 2.729 ± 0.032	3.11GluThr: 3.11 ± 0.045	3.745GluVal: 3.745 ± 0.052	0.675GluTrp: 0.675 ± 0.02	1.954GluTyr: 1.954 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	3.547PheAla: 3.547 ± 0.051	0.469PheCys: 0.469 ± 0.016	3.013PheAsp: 3.013 ± 0.042	2.862PheGlu: 2.862 ± 0.045	2.444PhePhe: 2.444 ± 0.047	3.557PheGly: 3.557 ± 0.053	0.699PheHis: 0.699 ± 0.022	3.714PheIle: 3.714 ± 0.059	3.797PheLys: 3.797 ± 0.054	4.492PheLeu: 4.492 ± 0.063	1.061PheMet: 1.061 ± 0.028	3.395PheAsn: 3.395 ± 0.056	1.664PhePro: 1.664 ± 0.032	1.324PheGln: 1.324 ± 0.027	1.639PheArg: 1.639 ± 0.032	3.848PheSer: 3.848 ± 0.05	3.221PheThr: 3.221 ± 0.046	2.975PheVal: 2.975 ± 0.044	0.654PheTrp: 0.654 ± 0.023	2.255PheTyr: 2.255 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	4.521GlyAla: 4.521 ± 0.063	0.561GlyCys: 0.561 ± 0.022	3.347GlyAsp: 3.347 ± 0.048	3.311GlyGlu: 3.311 ± 0.047	3.752GlyPhe: 3.752 ± 0.042	4.859GlyGly: 4.859 ± 0.084	1.047GlyHis: 1.047 ± 0.026	5.403GlyIle: 5.403 ± 0.059	5.738GlyLys: 5.738 ± 0.072	6.34GlyLeu: 6.34 ± 0.061	1.574GlyMet: 1.574 ± 0.03	4.082GlyAsn: 4.082 ± 0.06	1.498GlyPro: 1.498 ± 0.036	2.095GlyGln: 2.095 ± 0.038	2.278GlyArg: 2.278 ± 0.039	4.297GlySer: 4.297 ± 0.059	4.252GlyThr: 4.252 ± 0.065	4.311GlyVal: 4.311 ± 0.052	0.917GlyTrp: 0.917 ± 0.026	3.068GlyTyr: 3.068 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	0.89HisAla: 0.89 ± 0.025	0.164HisCys: 0.164 ± 0.011	0.782HisAsp: 0.782 ± 0.022	0.803HisGlu: 0.803 ± 0.025	1.068HisPhe: 1.068 ± 0.025	0.971HisGly: 0.971 ± 0.025	0.438HisHis: 0.438 ± 0.017	1.314HisIle: 1.314 ± 0.026	1.003HisLys: 1.003 ± 0.024	1.702HisLeu: 1.702 ± 0.031	0.269HisMet: 0.269 ± 0.014	0.842HisAsn: 0.842 ± 0.022	0.858HisPro: 0.858 ± 0.022	0.8HisGln: 0.8 ± 0.026	0.631HisArg: 0.631 ± 0.02	0.94HisSer: 0.94 ± 0.021	0.894HisThr: 0.894 ± 0.023	0.813HisVal: 0.813 ± 0.02	0.222HisTrp: 0.222 ± 0.012	0.758HisTyr: 0.758 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.036IleAla: 6.036 ± 0.07	0.691IleCys: 0.691 ± 0.021	4.485IleAsp: 4.485 ± 0.062	4.538IleGlu: 4.538 ± 0.057	3.362IlePhe: 3.362 ± 0.06	5.052IleGly: 5.052 ± 0.061	1.181IleHis: 1.181 ± 0.029	5.541IleIle: 5.541 ± 0.076	5.935IleLys: 5.935 ± 0.066	6.569IleLeu: 6.569 ± 0.071	1.338IleMet: 1.338 ± 0.029	4.95IleAsn: 4.95 ± 0.059	3.081IlePro: 3.081 ± 0.04	2.203IleGln: 2.203 ± 0.036	2.56IleArg: 2.56 ± 0.042	5.422IleSer: 5.422 ± 0.059	4.799IleThr: 4.799 ± 0.062	4.354IleVal: 4.354 ± 0.053	0.768IleTrp: 0.768 ± 0.023	2.832IleTyr: 2.832 ± 0.043	0.002IleXaa: 0.002 ± 0.001
Lys	5.668LysAla: 5.668 ± 0.063	0.324LysCys: 0.324 ± 0.015	4.563LysAsp: 4.563 ± 0.062	4.985LysGlu: 4.985 ± 0.067	3.117LysPhe: 3.117 ± 0.044	4.962LysGly: 4.962 ± 0.055	1.283LysHis: 1.283 ± 0.031	5.979LysIle: 5.979 ± 0.06	6.259LysLys: 6.259 ± 0.073	6.868LysLeu: 6.868 ± 0.071	2.024LysMet: 2.024 ± 0.035	5.046LysAsn: 5.046 ± 0.058	2.938LysPro: 2.938 ± 0.043	2.921LysGln: 2.921 ± 0.046	2.686LysArg: 2.686 ± 0.043	4.586LysSer: 4.586 ± 0.061	4.824LysThr: 4.824 ± 0.057	4.932LysVal: 4.932 ± 0.053	0.906LysTrp: 0.906 ± 0.022	3.15LysTyr: 3.15 ± 0.039	0.0LysXaa: 0.0 ± 0.0
Leu	7.01LeuAla: 7.01 ± 0.079	0.759LeuCys: 0.759 ± 0.023	4.693LeuAsp: 4.693 ± 0.059	4.772LeuGlu: 4.772 ± 0.063	4.71LeuPhe: 4.71 ± 0.072	5.75LeuGly: 5.75 ± 0.063	1.472LeuHis: 1.472 ± 0.033	6.902LeuIle: 6.902 ± 0.083	7.931LeuLys: 7.931 ± 0.073	9.103LeuLeu: 9.103 ± 0.109	2.094LeuMet: 2.094 ± 0.039	6.171LeuAsn: 6.171 ± 0.071	3.89LeuPro: 3.89 ± 0.055	3.303LeuGln: 3.303 ± 0.047	3.288LeuArg: 3.288 ± 0.046	6.893LeuSer: 6.893 ± 0.07	5.804LeuThr: 5.804 ± 0.059	5.408LeuVal: 5.408 ± 0.065	0.93LeuTrp: 0.93 ± 0.027	3.244LeuTyr: 3.244 ± 0.052	0.001LeuXaa: 0.001 ± 0.0
Met	1.716MetAla: 1.716 ± 0.037	0.133MetCys: 0.133 ± 0.009	1.101MetAsp: 1.101 ± 0.023	1.292MetGlu: 1.292 ± 0.027	0.89MetPhe: 0.89 ± 0.022	1.484MetGly: 1.484 ± 0.027	0.364MetHis: 0.364 ± 0.014	1.493MetIle: 1.493 ± 0.031	2.057MetLys: 2.057 ± 0.036	2.01MetLeu: 2.01 ± 0.038	0.599MetMet: 0.599 ± 0.018	1.281MetAsn: 1.281 ± 0.026	0.983MetPro: 0.983 ± 0.027	0.851MetGln: 0.851 ± 0.022	0.86MetArg: 0.86 ± 0.024	1.293MetSer: 1.293 ± 0.026	1.049MetThr: 1.049 ± 0.026	1.42MetVal: 1.42 ± 0.031	0.195MetTrp: 0.195 ± 0.01	0.685MetTyr: 0.685 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	4.443AsnAla: 4.443 ± 0.056	0.426AsnCys: 0.426 ± 0.018	3.065AsnAsp: 3.065 ± 0.048	3.364AsnGlu: 3.364 ± 0.055	3.124AsnPhe: 3.124 ± 0.051	4.695AsnGly: 4.695 ± 0.071	1.04AsnHis: 1.04 ± 0.026	4.606AsnIle: 4.606 ± 0.064	4.387AsnLys: 4.387 ± 0.056	5.767AsnLeu: 5.767 ± 0.071	1.237AsnMet: 1.237 ± 0.026	3.841AsnAsn: 3.841 ± 0.069	2.889AsnPro: 2.889 ± 0.047	2.382AsnGln: 2.382 ± 0.035	2.247AsnArg: 2.247 ± 0.037	3.758AsnSer: 3.758 ± 0.055	3.542AsnThr: 3.542 ± 0.056	3.697AsnVal: 3.697 ± 0.049	0.875AsnTrp: 0.875 ± 0.026	3.057AsnTyr: 3.057 ± 0.052	0.0AsnXaa: 0.0 ± 0.0
Pro	2.725ProAla: 2.725 ± 0.04	0.21ProCys: 0.21 ± 0.013	2.142ProAsp: 2.142 ± 0.038	2.453ProGlu: 2.453 ± 0.039	1.95ProPhe: 1.95 ± 0.034	2.352ProGly: 2.352 ± 0.038	0.572ProHis: 0.572 ± 0.021	2.775ProIle: 2.775 ± 0.04	2.57ProLys: 2.57 ± 0.042	3.217ProLeu: 3.217 ± 0.039	0.785ProMet: 0.785 ± 0.023	2.296ProAsn: 2.296 ± 0.037	0.899ProPro: 0.899 ± 0.027	1.295ProGln: 1.295 ± 0.029	1.01ProArg: 1.01 ± 0.026	2.42ProSer: 2.42 ± 0.041	2.278ProThr: 2.278 ± 0.043	2.555ProVal: 2.555 ± 0.041	0.388ProTrp: 0.388 ± 0.014	1.45ProTyr: 1.45 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	2.456GlnAla: 2.456 ± 0.041	0.167GlnCys: 0.167 ± 0.011	1.57GlnAsp: 1.57 ± 0.031	1.932GlnGlu: 1.932 ± 0.038	1.726GlnPhe: 1.726 ± 0.029	1.976GlnGly: 1.976 ± 0.034	0.616GlnHis: 0.616 ± 0.019	2.626GlnIle: 2.626 ± 0.04	2.837GlnLys: 2.837 ± 0.038	3.701GlnLeu: 3.701 ± 0.046	0.862GlnMet: 0.862 ± 0.023	2.263GlnAsn: 2.263 ± 0.042	1.25GlnPro: 1.25 ± 0.027	1.766GlnGln: 1.766 ± 0.046	1.348GlnArg: 1.348 ± 0.027	2.121GlnSer: 2.121 ± 0.037	2.203GlnThr: 2.203 ± 0.038	2.191GlnVal: 2.191 ± 0.035	0.4GlnTrp: 0.4 ± 0.015	1.415GlnTyr: 1.415 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.22ArgAla: 2.22 ± 0.04	0.207ArgCys: 0.207 ± 0.009	1.753ArgAsp: 1.753 ± 0.036	1.984ArgGlu: 1.984 ± 0.04	1.99ArgPhe: 1.99 ± 0.037	2.052ArgGly: 2.052 ± 0.04	0.531ArgHis: 0.531 ± 0.02	2.764ArgIle: 2.764 ± 0.038	2.691ArgLys: 2.691 ± 0.046	3.47ArgLeu: 3.47 ± 0.048	0.906ArgMet: 0.906 ± 0.024	2.211ArgAsn: 2.211 ± 0.037	1.213ArgPro: 1.213 ± 0.026	1.13ArgGln: 1.13 ± 0.025	1.306ArgArg: 1.306 ± 0.037	1.966ArgSer: 1.966 ± 0.038	1.95ArgThr: 1.95 ± 0.034	2.108ArgVal: 2.108 ± 0.041	0.443ArgTrp: 0.443 ± 0.016	1.616ArgTyr: 1.616 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	4.558SerAla: 4.558 ± 0.058	0.542SerCys: 0.542 ± 0.019	3.011SerAsp: 3.011 ± 0.039	3.009SerGlu: 3.009 ± 0.045	3.853SerPhe: 3.853 ± 0.054	4.649SerGly: 4.649 ± 0.063	1.022SerHis: 1.022 ± 0.026	5.168SerIle: 5.168 ± 0.065	4.441SerLys: 4.441 ± 0.048	6.384SerLeu: 6.384 ± 0.07	1.223SerMet: 1.223 ± 0.024	3.629SerAsn: 3.629 ± 0.056	2.279SerPro: 2.279 ± 0.037	1.986SerGln: 1.986 ± 0.035	2.145SerArg: 2.145 ± 0.033	4.375SerSer: 4.375 ± 0.058	3.839SerThr: 3.839 ± 0.058	3.939SerVal: 3.939 ± 0.059	0.77SerTrp: 0.77 ± 0.022	2.971SerTyr: 2.971 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	4.683ThrAla: 4.683 ± 0.067	0.331ThrCys: 0.331 ± 0.015	3.302ThrAsp: 3.302 ± 0.039	3.146ThrGlu: 3.146 ± 0.046	2.948ThrPhe: 2.948 ± 0.044	4.549ThrGly: 4.549 ± 0.06	0.897ThrHis: 0.897 ± 0.022	4.645ThrIle: 4.645 ± 0.06	4.055ThrLys: 4.055 ± 0.047	5.551ThrLeu: 5.551 ± 0.067	1.013ThrMet: 1.013 ± 0.024	3.426ThrAsn: 3.426 ± 0.056	2.572ThrPro: 2.572 ± 0.042	1.977ThrGln: 1.977 ± 0.035	1.707ThrArg: 1.707 ± 0.029	3.8ThrSer: 3.8 ± 0.064	3.842ThrThr: 3.842 ± 0.072	3.832ThrVal: 3.832 ± 0.064	0.713ThrTrp: 0.713 ± 0.023	2.627ThrTyr: 2.627 ± 0.047	0.001ThrXaa: 0.001 ± 0.0
Val	4.508ValAla: 4.508 ± 0.062	0.491ValCys: 0.491 ± 0.017	3.348ValAsp: 3.348 ± 0.05	3.349ValGlu: 3.349 ± 0.041	3.133ValPhe: 3.133 ± 0.044	3.904ValGly: 3.904 ± 0.053	0.881ValHis: 0.881 ± 0.021	4.598ValIle: 4.598 ± 0.056	5.025ValLys: 5.025 ± 0.06	5.641ValLeu: 5.641 ± 0.06	1.358ValMet: 1.358 ± 0.03	4.117ValAsn: 4.117 ± 0.052	2.227ValPro: 2.227 ± 0.036	1.892ValGln: 1.892 ± 0.036	2.049ValArg: 2.049 ± 0.04	4.282ValSer: 4.282 ± 0.058	3.604ValThr: 3.604 ± 0.071	4.124ValVal: 4.124 ± 0.059	0.71ValTrp: 0.71 ± 0.024	2.477ValTyr: 2.477 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.801TrpAla: 0.801 ± 0.021	0.116TrpCys: 0.116 ± 0.009	0.708TrpAsp: 0.708 ± 0.024	0.682TrpGlu: 0.682 ± 0.02	0.602TrpPhe: 0.602 ± 0.018	0.83TrpGly: 0.83 ± 0.027	0.225TrpHis: 0.225 ± 0.012	0.771TrpIle: 0.771 ± 0.025	0.9TrpLys: 0.9 ± 0.023	1.123TrpLeu: 1.123 ± 0.027	0.335TrpMet: 0.335 ± 0.016	0.749TrpAsn: 0.749 ± 0.021	0.368TrpPro: 0.368 ± 0.017	0.498TrpGln: 0.498 ± 0.018	0.47TrpArg: 0.47 ± 0.015	0.713TrpSer: 0.713 ± 0.021	0.648TrpThr: 0.648 ± 0.021	0.721TrpVal: 0.721 ± 0.02	0.209TrpTrp: 0.209 ± 0.012	0.491TrpTyr: 0.491 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.906TyrAla: 2.906 ± 0.043	0.325TyrCys: 0.325 ± 0.015	2.226TyrAsp: 2.226 ± 0.036	1.966TyrGlu: 1.966 ± 0.039	2.4TyrPhe: 2.4 ± 0.04	2.836TyrGly: 2.836 ± 0.048	0.845TyrHis: 0.845 ± 0.023	2.591TyrIle: 2.591 ± 0.039	2.993TyrLys: 2.993 ± 0.044	4.011TyrLeu: 4.011 ± 0.052	0.76TyrMet: 0.76 ± 0.023	2.754TyrAsn: 2.754 ± 0.055	1.709TyrPro: 1.709 ± 0.031	1.832TyrGln: 1.832 ± 0.029	1.716TyrArg: 1.716 ± 0.031	2.621TyrSer: 2.621 ± 0.046	2.562TyrThr: 2.562 ± 0.044	2.258TyrVal: 2.258 ± 0.043	0.544TyrTrp: 0.544 ± 0.02	1.874TyrTyr: 1.874 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 4698 proteins (1709408 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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