Mycobacterium phage Phatniss

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1Y709|A0A0K1Y709_9CAUD Uncharacterized protein OS=Mycobacterium phage Phatniss OX=1698356 GN=PHATNISS_52 PE=4 SV=1
MM1 pKa = 7.92SGEE4 pKa = 4.04INPEE8 pKa = 3.43GFTRR12 pKa = 11.84YY13 pKa = 10.28GGDD16 pKa = 3.72CTCGPIYY23 pKa = 10.21TYY25 pKa = 10.79GGHH28 pKa = 7.13AEE30 pKa = 4.48PGQFDD35 pKa = 4.88PFCPDD40 pKa = 2.8HH41 pKa = 6.95GNPEE45 pKa = 4.29YY46 pKa = 10.83VASLEE51 pKa = 4.27EE52 pKa = 4.01AA53 pKa = 3.67

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1Y756|A0A0K1Y756_9CAUD Uncharacterized protein OS=Mycobacterium phage Phatniss OX=1698356 GN=PHATNISS_97 PE=4 SV=1
MM1 pKa = 7.8AGTAVLTPDD10 pKa = 5.37GIDD13 pKa = 3.45TLVTAAEE20 pKa = 4.08AASLCGVTTSTIYY33 pKa = 10.15VWVNRR38 pKa = 11.84GTLAPSGKK46 pKa = 9.45NRR48 pKa = 11.84LGHH51 pKa = 5.16NVYY54 pKa = 10.31RR55 pKa = 11.84VLDD58 pKa = 3.67VAKK61 pKa = 10.59AEE63 pKa = 4.07HH64 pKa = 6.08ATRR67 pKa = 11.84AKK69 pKa = 10.42ARR71 pKa = 11.84RR72 pKa = 11.84HH73 pKa = 4.63RR74 pKa = 3.93

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

17925

30

1163

177.5

19.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.628 ± 0.475

1.266 ± 0.159

6.656 ± 0.228

5.886 ± 0.324

3.079 ± 0.19

8.87 ± 0.534

2.377 ± 0.193

4.279 ± 0.208

3.381 ± 0.17

7.091 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.14

3.392 ± 0.182

6.103 ± 0.214

3.358 ± 0.207

6.84 ± 0.378

5.729 ± 0.265

6.762 ± 0.265

7.141 ± 0.263

2.371 ± 0.15

2.577 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski