Amino acid dipepetide frequency for Mycobacterium phage Phatniss

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.227AlaAla: 14.227 ± 1.583
1.06AlaCys: 1.06 ± 0.212
6.862AlaAsp: 6.862 ± 0.661
7.085AlaGlu: 7.085 ± 0.679
2.455AlaPhe: 2.455 ± 0.409
10.266AlaGly: 10.266 ± 1.21
2.901AlaHis: 2.901 ± 0.476
4.296AlaIle: 4.296 ± 0.54
4.352AlaLys: 4.352 ± 0.455
7.141AlaLeu: 7.141 ± 0.662
2.622AlaMet: 2.622 ± 0.413
2.901AlaAsn: 2.901 ± 0.425
5.412AlaPro: 5.412 ± 0.628
3.794AlaGln: 3.794 ± 0.477
7.643AlaArg: 7.643 ± 0.751
4.575AlaSer: 4.575 ± 0.652
6.807AlaThr: 6.807 ± 0.57
7.03AlaVal: 7.03 ± 0.588
2.566AlaTrp: 2.566 ± 0.382
2.287AlaTyr: 2.287 ± 0.3
0.0AlaXaa: 0.0 ± 0.0
Cys
1.172CysAla: 1.172 ± 0.287
0.112CysCys: 0.112 ± 0.071
1.339CysAsp: 1.339 ± 0.331
0.893CysGlu: 0.893 ± 0.243
0.335CysPhe: 0.335 ± 0.134
1.73CysGly: 1.73 ± 0.331
0.279CysHis: 0.279 ± 0.133
0.112CysIle: 0.112 ± 0.075
0.446CysLys: 0.446 ± 0.146
0.502CysLeu: 0.502 ± 0.164
0.112CysMet: 0.112 ± 0.075
0.391CysAsn: 0.391 ± 0.142
1.004CysPro: 1.004 ± 0.254
0.335CysGln: 0.335 ± 0.168
0.669CysArg: 0.669 ± 0.197
0.781CysSer: 0.781 ± 0.222
0.893CysThr: 0.893 ± 0.23
0.837CysVal: 0.837 ± 0.207
0.446CysTrp: 0.446 ± 0.132
0.279CysTyr: 0.279 ± 0.121
0.0CysXaa: 0.0 ± 0.0
Asp
6.639AspAla: 6.639 ± 0.66
0.948AspCys: 0.948 ± 0.201
4.519AspAsp: 4.519 ± 0.476
3.347AspGlu: 3.347 ± 0.523
1.785AspPhe: 1.785 ± 0.258
6.695AspGly: 6.695 ± 0.568
1.506AspHis: 1.506 ± 0.264
2.064AspIle: 2.064 ± 0.293
1.785AspLys: 1.785 ± 0.267
5.244AspLeu: 5.244 ± 0.474
1.004AspMet: 1.004 ± 0.244
1.785AspAsn: 1.785 ± 0.361
5.468AspPro: 5.468 ± 0.613
1.953AspGln: 1.953 ± 0.263
5.133AspArg: 5.133 ± 0.799
3.459AspSer: 3.459 ± 0.529
4.798AspThr: 4.798 ± 0.567
4.519AspVal: 4.519 ± 0.647
1.73AspTrp: 1.73 ± 0.26
2.176AspTyr: 2.176 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
6.249GluAla: 6.249 ± 0.704
0.948GluCys: 0.948 ± 0.232
3.124GluAsp: 3.124 ± 0.373
2.79GluGlu: 2.79 ± 0.601
2.232GluPhe: 2.232 ± 0.342
3.571GluGly: 3.571 ± 0.4
1.841GluHis: 1.841 ± 0.425
2.232GluIle: 2.232 ± 0.412
1.953GluLys: 1.953 ± 0.31
5.97GluLeu: 5.97 ± 0.652
1.451GluMet: 1.451 ± 0.309
2.12GluAsn: 2.12 ± 0.277
3.124GluPro: 3.124 ± 0.448
2.622GluGln: 2.622 ± 0.4
4.91GluArg: 4.91 ± 0.659
3.18GluSer: 3.18 ± 0.471
4.184GluThr: 4.184 ± 0.492
3.459GluVal: 3.459 ± 0.489
1.227GluTrp: 1.227 ± 0.245
1.674GluTyr: 1.674 ± 0.286
0.0GluXaa: 0.0 ± 0.0
Phe
3.18PheAla: 3.18 ± 0.386
0.223PheCys: 0.223 ± 0.095
2.455PheAsp: 2.455 ± 0.456
1.674PheGlu: 1.674 ± 0.324
0.893PhePhe: 0.893 ± 0.281
2.957PheGly: 2.957 ± 0.579
0.391PheHis: 0.391 ± 0.125
1.618PheIle: 1.618 ± 0.338
1.172PheLys: 1.172 ± 0.236
1.785PheLeu: 1.785 ± 0.263
0.614PheMet: 0.614 ± 0.197
1.506PheAsn: 1.506 ± 0.321
1.618PhePro: 1.618 ± 0.321
1.116PheGln: 1.116 ± 0.311
1.506PheArg: 1.506 ± 0.245
1.562PheSer: 1.562 ± 0.287
2.79PheThr: 2.79 ± 0.346
2.12PheVal: 2.12 ± 0.274
0.725PheTrp: 0.725 ± 0.16
0.893PheTyr: 0.893 ± 0.233
0.0PheXaa: 0.0 ± 0.0
Gly
8.424GlyAla: 8.424 ± 1.038
1.506GlyCys: 1.506 ± 0.339
5.97GlyAsp: 5.97 ± 0.566
4.184GlyGlu: 4.184 ± 0.505
2.845GlyPhe: 2.845 ± 0.437
10.545GlyGly: 10.545 ± 2.032
2.399GlyHis: 2.399 ± 0.328
3.682GlyIle: 3.682 ± 0.578
2.566GlyLys: 2.566 ± 0.382
5.802GlyLeu: 5.802 ± 0.573
2.566GlyMet: 2.566 ± 0.447
3.069GlyAsn: 3.069 ± 0.412
3.961GlyPro: 3.961 ± 0.472
1.953GlyGln: 1.953 ± 0.479
5.133GlyArg: 5.133 ± 0.545
5.97GlySer: 5.97 ± 0.811
6.528GlyThr: 6.528 ± 0.715
6.583GlyVal: 6.583 ± 0.536
2.566GlyTrp: 2.566 ± 0.357
2.455GlyTyr: 2.455 ± 0.484
0.0GlyXaa: 0.0 ± 0.0
His
2.176HisAla: 2.176 ± 0.385
0.446HisCys: 0.446 ± 0.171
1.395HisAsp: 1.395 ± 0.278
1.116HisGlu: 1.116 ± 0.343
0.391HisPhe: 0.391 ± 0.147
2.008HisGly: 2.008 ± 0.357
0.893HisHis: 0.893 ± 0.232
1.73HisIle: 1.73 ± 0.31
0.893HisLys: 0.893 ± 0.253
1.841HisLeu: 1.841 ± 0.342
0.502HisMet: 0.502 ± 0.172
1.004HisAsn: 1.004 ± 0.224
1.562HisPro: 1.562 ± 0.243
0.502HisGln: 0.502 ± 0.169
2.287HisArg: 2.287 ± 0.408
0.893HisSer: 0.893 ± 0.199
1.283HisThr: 1.283 ± 0.295
1.562HisVal: 1.562 ± 0.339
0.391HisTrp: 0.391 ± 0.133
0.837HisTyr: 0.837 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
5.3IleAla: 5.3 ± 0.604
0.669IleCys: 0.669 ± 0.228
3.85IleAsp: 3.85 ± 0.488
3.236IleGlu: 3.236 ± 0.338
0.781IlePhe: 0.781 ± 0.216
3.682IleGly: 3.682 ± 0.552
1.339IleHis: 1.339 ± 0.271
1.562IleIle: 1.562 ± 0.289
1.227IleLys: 1.227 ± 0.287
2.008IleLeu: 2.008 ± 0.343
0.391IleMet: 0.391 ± 0.139
1.897IleAsn: 1.897 ± 0.318
2.678IlePro: 2.678 ± 0.344
1.451IleGln: 1.451 ± 0.204
2.287IleArg: 2.287 ± 0.379
2.232IleSer: 2.232 ± 0.363
3.18IleThr: 3.18 ± 0.374
2.901IleVal: 2.901 ± 0.379
1.116IleTrp: 1.116 ± 0.277
1.004IleTyr: 1.004 ± 0.265
0.0IleXaa: 0.0 ± 0.0
Lys
3.961LysAla: 3.961 ± 0.573
0.391LysCys: 0.391 ± 0.145
1.674LysAsp: 1.674 ± 0.298
1.506LysGlu: 1.506 ± 0.272
1.339LysPhe: 1.339 ± 0.225
2.79LysGly: 2.79 ± 0.352
1.116LysHis: 1.116 ± 0.221
0.837LysIle: 0.837 ± 0.245
1.506LysLys: 1.506 ± 0.438
2.566LysLeu: 2.566 ± 0.544
0.725LysMet: 0.725 ± 0.204
0.948LysAsn: 0.948 ± 0.259
2.343LysPro: 2.343 ± 0.414
1.841LysGln: 1.841 ± 0.284
2.343LysArg: 2.343 ± 0.356
1.785LysSer: 1.785 ± 0.319
1.897LysThr: 1.897 ± 0.356
2.622LysVal: 2.622 ± 0.424
0.669LysTrp: 0.669 ± 0.229
0.948LysTyr: 0.948 ± 0.277
0.0LysXaa: 0.0 ± 0.0
Leu
7.532LeuAla: 7.532 ± 0.798
0.781LeuCys: 0.781 ± 0.233
4.686LeuAsp: 4.686 ± 0.601
3.738LeuGlu: 3.738 ± 0.471
2.566LeuPhe: 2.566 ± 0.294
4.91LeuGly: 4.91 ± 0.523
1.06LeuHis: 1.06 ± 0.267
2.901LeuIle: 2.901 ± 0.339
2.287LeuLys: 2.287 ± 0.314
4.742LeuLeu: 4.742 ± 0.501
1.506LeuMet: 1.506 ± 0.293
2.511LeuAsn: 2.511 ± 0.338
5.021LeuPro: 5.021 ± 0.705
2.678LeuGln: 2.678 ± 0.442
5.189LeuArg: 5.189 ± 0.564
5.021LeuSer: 5.021 ± 0.479
5.579LeuThr: 5.579 ± 0.447
4.742LeuVal: 4.742 ± 0.572
1.283LeuTrp: 1.283 ± 0.347
2.176LeuTyr: 2.176 ± 0.378
0.0LeuXaa: 0.0 ± 0.0
Met
2.455MetAla: 2.455 ± 0.326
0.335MetCys: 0.335 ± 0.171
1.116MetAsp: 1.116 ± 0.258
1.227MetGlu: 1.227 ± 0.229
0.781MetPhe: 0.781 ± 0.23
1.897MetGly: 1.897 ± 0.325
0.112MetHis: 0.112 ± 0.087
0.837MetIle: 0.837 ± 0.221
0.669MetLys: 0.669 ± 0.246
1.897MetLeu: 1.897 ± 0.248
0.446MetMet: 0.446 ± 0.225
0.725MetAsn: 0.725 ± 0.168
1.227MetPro: 1.227 ± 0.24
0.502MetGln: 0.502 ± 0.155
1.339MetArg: 1.339 ± 0.272
2.511MetSer: 2.511 ± 0.373
2.176MetThr: 2.176 ± 0.306
1.172MetVal: 1.172 ± 0.323
0.335MetTrp: 0.335 ± 0.132
0.391MetTyr: 0.391 ± 0.128
0.0MetXaa: 0.0 ± 0.0
Asn
3.347AsnAla: 3.347 ± 0.417
0.279AsnCys: 0.279 ± 0.139
1.841AsnAsp: 1.841 ± 0.307
1.897AsnGlu: 1.897 ± 0.385
0.893AsnPhe: 0.893 ± 0.268
4.24AsnGly: 4.24 ± 0.581
0.948AsnHis: 0.948 ± 0.172
1.618AsnIle: 1.618 ± 0.473
1.004AsnLys: 1.004 ± 0.282
2.343AsnLeu: 2.343 ± 0.327
0.614AsnMet: 0.614 ± 0.161
1.785AsnAsn: 1.785 ± 0.346
2.957AsnPro: 2.957 ± 0.354
1.172AsnGln: 1.172 ± 0.326
2.064AsnArg: 2.064 ± 0.451
1.618AsnSer: 1.618 ± 0.332
1.841AsnThr: 1.841 ± 0.295
1.785AsnVal: 1.785 ± 0.329
0.893AsnTrp: 0.893 ± 0.177
0.781AsnTyr: 0.781 ± 0.172
0.0AsnXaa: 0.0 ± 0.0
Pro
5.691ProAla: 5.691 ± 0.686
0.558ProCys: 0.558 ± 0.168
4.407ProAsp: 4.407 ± 0.47
4.798ProGlu: 4.798 ± 0.46
1.953ProPhe: 1.953 ± 0.379
6.528ProGly: 6.528 ± 0.626
1.283ProHis: 1.283 ± 0.283
2.064ProIle: 2.064 ± 0.398
2.343ProLys: 2.343 ± 0.459
4.129ProLeu: 4.129 ± 0.57
1.339ProMet: 1.339 ± 0.301
2.176ProAsn: 2.176 ± 0.37
4.686ProPro: 4.686 ± 0.638
2.008ProGln: 2.008 ± 0.316
3.18ProArg: 3.18 ± 0.476
3.292ProSer: 3.292 ± 0.427
3.292ProThr: 3.292 ± 0.446
4.686ProVal: 4.686 ± 0.593
1.116ProTrp: 1.116 ± 0.219
1.506ProTyr: 1.506 ± 0.313
0.0ProXaa: 0.0 ± 0.0
Gln
4.24GlnAla: 4.24 ± 0.6
0.167GlnCys: 0.167 ± 0.124
1.618GlnAsp: 1.618 ± 0.241
1.785GlnGlu: 1.785 ± 0.342
1.06GlnPhe: 1.06 ± 0.25
2.232GlnGly: 2.232 ± 0.394
0.893GlnHis: 0.893 ± 0.225
1.785GlnIle: 1.785 ± 0.282
1.339GlnLys: 1.339 ± 0.23
2.845GlnLeu: 2.845 ± 0.407
0.614GlnMet: 0.614 ± 0.177
0.837GlnAsn: 0.837 ± 0.204
2.064GlnPro: 2.064 ± 0.336
1.06GlnGln: 1.06 ± 0.226
2.566GlnArg: 2.566 ± 0.317
2.455GlnSer: 2.455 ± 0.339
1.841GlnThr: 1.841 ± 0.381
2.566GlnVal: 2.566 ± 0.398
0.781GlnTrp: 0.781 ± 0.223
0.837GlnTyr: 0.837 ± 0.229
0.0GlnXaa: 0.0 ± 0.0
Arg
7.253ArgAla: 7.253 ± 0.668
1.395ArgCys: 1.395 ± 0.368
4.519ArgAsp: 4.519 ± 0.625
5.021ArgGlu: 5.021 ± 0.76
2.455ArgPhe: 2.455 ± 0.419
3.85ArgGly: 3.85 ± 0.443
1.451ArgHis: 1.451 ± 0.355
4.129ArgIle: 4.129 ± 0.47
2.455ArgLys: 2.455 ± 0.377
4.017ArgLeu: 4.017 ± 0.494
2.511ArgMet: 2.511 ± 0.371
2.566ArgAsn: 2.566 ± 0.451
3.236ArgPro: 3.236 ± 0.402
2.064ArgGln: 2.064 ± 0.3
5.133ArgArg: 5.133 ± 0.668
3.794ArgSer: 3.794 ± 0.373
3.403ArgThr: 3.403 ± 0.441
5.133ArgVal: 5.133 ± 0.6
2.008ArgTrp: 2.008 ± 0.346
2.008ArgTyr: 2.008 ± 0.283
0.0ArgXaa: 0.0 ± 0.0
Ser
5.635SerAla: 5.635 ± 0.77
0.502SerCys: 0.502 ± 0.183
4.073SerAsp: 4.073 ± 0.544
3.236SerGlu: 3.236 ± 0.435
2.064SerPhe: 2.064 ± 0.452
5.97SerGly: 5.97 ± 0.9
1.116SerHis: 1.116 ± 0.181
2.455SerIle: 2.455 ± 0.404
2.455SerLys: 2.455 ± 0.358
3.626SerLeu: 3.626 ± 0.489
1.172SerMet: 1.172 ± 0.235
2.176SerAsn: 2.176 ± 0.514
3.236SerPro: 3.236 ± 0.317
1.674SerGln: 1.674 ± 0.307
3.794SerArg: 3.794 ± 0.439
3.515SerSer: 3.515 ± 0.547
3.682SerThr: 3.682 ± 0.423
4.129SerVal: 4.129 ± 0.539
1.339SerTrp: 1.339 ± 0.253
1.451SerTyr: 1.451 ± 0.289
0.0SerXaa: 0.0 ± 0.0
Thr
6.695ThrAla: 6.695 ± 0.573
0.781ThrCys: 0.781 ± 0.218
4.519ThrAsp: 4.519 ± 0.561
3.682ThrGlu: 3.682 ± 0.383
1.953ThrPhe: 1.953 ± 0.312
6.137ThrGly: 6.137 ± 0.565
1.953ThrHis: 1.953 ± 0.32
3.515ThrIle: 3.515 ± 0.415
1.841ThrLys: 1.841 ± 0.347
5.021ThrLeu: 5.021 ± 0.549
1.395ThrMet: 1.395 ± 0.272
2.12ThrAsn: 2.12 ± 0.34
4.742ThrPro: 4.742 ± 0.628
1.841ThrGln: 1.841 ± 0.3
4.24ThrArg: 4.24 ± 0.501
3.905ThrSer: 3.905 ± 0.424
4.91ThrThr: 4.91 ± 0.609
5.579ThrVal: 5.579 ± 0.613
1.06ThrTrp: 1.06 ± 0.295
1.73ThrTyr: 1.73 ± 0.261
0.0ThrXaa: 0.0 ± 0.0
Val
7.364ValAla: 7.364 ± 0.535
1.116ValCys: 1.116 ± 0.267
5.021ValAsp: 5.021 ± 0.498
4.519ValGlu: 4.519 ± 0.513
2.511ValPhe: 2.511 ± 0.371
5.468ValGly: 5.468 ± 0.647
1.283ValHis: 1.283 ± 0.255
2.845ValIle: 2.845 ± 0.385
2.232ValLys: 2.232 ± 0.369
5.021ValLeu: 5.021 ± 0.56
1.339ValMet: 1.339 ± 0.206
2.287ValAsn: 2.287 ± 0.351
4.24ValPro: 4.24 ± 0.429
3.18ValGln: 3.18 ± 0.4
4.296ValArg: 4.296 ± 0.493
4.24ValSer: 4.24 ± 0.592
5.077ValThr: 5.077 ± 0.593
6.081ValVal: 6.081 ± 0.763
1.897ValTrp: 1.897 ± 0.329
1.395ValTyr: 1.395 ± 0.289
0.0ValXaa: 0.0 ± 0.0
Trp
2.176TrpAla: 2.176 ± 0.286
0.223TrpCys: 0.223 ± 0.112
1.618TrpAsp: 1.618 ± 0.328
1.227TrpGlu: 1.227 ± 0.325
0.558TrpPhe: 0.558 ± 0.15
1.116TrpGly: 1.116 ± 0.234
0.614TrpHis: 0.614 ± 0.158
1.283TrpIle: 1.283 ± 0.245
0.614TrpLys: 0.614 ± 0.174
2.064TrpLeu: 2.064 ± 0.365
0.893TrpMet: 0.893 ± 0.236
0.558TrpAsn: 0.558 ± 0.205
1.283TrpPro: 1.283 ± 0.268
1.116TrpGln: 1.116 ± 0.242
2.455TrpArg: 2.455 ± 0.437
1.339TrpSer: 1.339 ± 0.301
1.674TrpThr: 1.674 ± 0.291
1.562TrpVal: 1.562 ± 0.417
1.004TrpTrp: 1.004 ± 0.215
0.335TrpTyr: 0.335 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.566TyrAla: 2.566 ± 0.372
0.223TyrCys: 0.223 ± 0.107
1.953TyrAsp: 1.953 ± 0.337
1.897TyrGlu: 1.897 ± 0.267
0.948TyrPhe: 0.948 ± 0.24
2.12TyrGly: 2.12 ± 0.332
0.391TyrHis: 0.391 ± 0.134
1.227TyrIle: 1.227 ± 0.235
0.669TyrLys: 0.669 ± 0.207
2.343TyrLeu: 2.343 ± 0.366
0.279TyrMet: 0.279 ± 0.122
0.558TyrAsn: 0.558 ± 0.157
1.172TyrPro: 1.172 ± 0.208
0.725TyrGln: 0.725 ± 0.208
2.232TyrArg: 2.232 ± 0.402
1.172TyrSer: 1.172 ± 0.233
1.785TyrThr: 1.785 ± 0.353
2.343TyrVal: 2.343 ± 0.314
0.558TyrTrp: 0.558 ± 0.16
0.614TyrTyr: 0.614 ± 0.148
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (17925 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski