Gordonia phage Upyo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5FU02|A0A7D5FU02_9CAUD Uncharacterized protein OS=Gordonia phage Upyo OX=2743989 GN=62 PE=4 SV=1
MM1 pKa = 7.89SIRR4 pKa = 11.84PHH6 pKa = 5.56LTLAAVVVAVIITASACSAIGLGSEE31 pKa = 4.19AGEE34 pKa = 4.42SQFPDD39 pKa = 3.47PRR41 pKa = 11.84PYY43 pKa = 10.28ATVPAEE49 pKa = 3.88TSYY52 pKa = 8.62PTTTTEE58 pKa = 3.72EE59 pKa = 4.29TTVEE63 pKa = 3.75NTYY66 pKa = 10.12PLEE69 pKa = 3.97VQVYY73 pKa = 9.4IEE75 pKa = 4.12TARR78 pKa = 11.84DD79 pKa = 3.58LLDD82 pKa = 3.8SGAVPEE88 pKa = 4.36EE89 pKa = 4.34AVTTTAVQADD99 pKa = 4.84LIDD102 pKa = 3.99NYY104 pKa = 11.02GITVDD109 pKa = 4.47DD110 pKa = 3.77ATAAAICEE118 pKa = 4.26EE119 pKa = 4.2ILRR122 pKa = 4.38

Molecular weight: 12.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5FSX0|A0A7D5FSX0_9CAUD Uncharacterized protein OS=Gordonia phage Upyo OX=2743989 GN=49 PE=4 SV=1
MM1 pKa = 7.87SYY3 pKa = 10.87PRR5 pKa = 11.84MPGEE9 pKa = 3.98EE10 pKa = 3.94PLVRR14 pKa = 11.84RR15 pKa = 11.84LALQSVRR22 pKa = 11.84AEE24 pKa = 3.84TAAKK28 pKa = 9.31IRR30 pKa = 11.84GMVDD34 pKa = 2.76VMGGGVGLVLAMVLGSAVLNDD55 pKa = 3.72WTISWYY61 pKa = 10.34QIQGTVAGLILFGVGRR77 pKa = 11.84IWYY80 pKa = 8.58LRR82 pKa = 11.84HH83 pKa = 6.3RR84 pKa = 11.84LRR86 pKa = 11.84VVEE89 pKa = 4.04RR90 pKa = 11.84RR91 pKa = 11.84VGTGLDD97 pKa = 3.12PHH99 pKa = 6.7RR100 pKa = 11.84AKK102 pKa = 10.68EE103 pKa = 3.88

Molecular weight: 11.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

14796

31

1437

211.4

22.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.706 ± 0.471

0.676 ± 0.121

6.617 ± 0.378

5.711 ± 0.289

2.413 ± 0.159

8.678 ± 0.578

1.994 ± 0.188

4.258 ± 0.191

3.508 ± 0.202

7.739 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.132

2.994 ± 0.193

6.333 ± 0.348

3.373 ± 0.241

6.982 ± 0.356

5.177 ± 0.316

7.198 ± 0.272

7.698 ± 0.225

1.987 ± 0.158

2.494 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski