Amino acid dipepetide frequency for Gordonia phage Upyo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.802AlaAla: 14.802 ± 1.576
0.338AlaCys: 0.338 ± 0.138
9.125AlaAsp: 9.125 ± 1.078
7.435AlaGlu: 7.435 ± 0.722
2.23AlaPhe: 2.23 ± 0.426
9.125AlaGly: 9.125 ± 1.013
2.23AlaHis: 2.23 ± 0.456
4.055AlaIle: 4.055 ± 0.504
5.204AlaLys: 5.204 ± 0.458
8.787AlaLeu: 8.787 ± 0.775
3.042AlaMet: 3.042 ± 0.427
4.123AlaAsn: 4.123 ± 0.535
5.88AlaPro: 5.88 ± 0.796
4.393AlaGln: 4.393 ± 0.637
6.691AlaArg: 6.691 ± 0.752
6.353AlaSer: 6.353 ± 0.895
7.773AlaThr: 7.773 ± 0.759
9.868AlaVal: 9.868 ± 1.037
2.704AlaTrp: 2.704 ± 0.374
2.906AlaTyr: 2.906 ± 0.383
0.0AlaXaa: 0.0 ± 0.0
Cys
0.338CysAla: 0.338 ± 0.172
0.068CysCys: 0.068 ± 0.068
0.406CysAsp: 0.406 ± 0.163
0.473CysGlu: 0.473 ± 0.183
0.135CysPhe: 0.135 ± 0.089
0.811CysGly: 0.811 ± 0.231
0.203CysHis: 0.203 ± 0.116
0.27CysIle: 0.27 ± 0.151
0.203CysLys: 0.203 ± 0.114
0.27CysLeu: 0.27 ± 0.133
0.0CysMet: 0.0 ± 0.0
0.27CysAsn: 0.27 ± 0.141
0.473CysPro: 0.473 ± 0.182
0.406CysGln: 0.406 ± 0.143
0.541CysArg: 0.541 ± 0.191
0.811CysSer: 0.811 ± 0.259
0.541CysThr: 0.541 ± 0.188
0.27CysVal: 0.27 ± 0.119
0.203CysTrp: 0.203 ± 0.131
0.068CysTyr: 0.068 ± 0.065
0.0CysXaa: 0.0 ± 0.0
Asp
7.367AspAla: 7.367 ± 0.746
0.27AspCys: 0.27 ± 0.151
4.461AspAsp: 4.461 ± 0.93
3.582AspGlu: 3.582 ± 0.537
0.946AspPhe: 0.946 ± 0.193
6.083AspGly: 6.083 ± 0.623
1.487AspHis: 1.487 ± 0.314
2.839AspIle: 2.839 ± 0.465
1.757AspLys: 1.757 ± 0.387
5.745AspLeu: 5.745 ± 0.693
1.757AspMet: 1.757 ± 0.372
2.028AspAsn: 2.028 ± 0.387
5.813AspPro: 5.813 ± 0.865
1.96AspGln: 1.96 ± 0.375
4.461AspArg: 4.461 ± 0.519
2.095AspSer: 2.095 ± 0.317
4.867AspThr: 4.867 ± 0.542
4.529AspVal: 4.529 ± 0.537
2.095AspTrp: 2.095 ± 0.336
2.028AspTyr: 2.028 ± 0.387
0.0AspXaa: 0.0 ± 0.0
Glu
6.759GluAla: 6.759 ± 0.771
0.541GluCys: 0.541 ± 0.212
4.596GluAsp: 4.596 ± 0.717
3.853GluGlu: 3.853 ± 0.497
1.893GluPhe: 1.893 ± 0.382
3.244GluGly: 3.244 ± 0.514
1.081GluHis: 1.081 ± 0.263
2.974GluIle: 2.974 ± 0.382
0.811GluLys: 0.811 ± 0.189
5.204GluLeu: 5.204 ± 0.592
1.352GluMet: 1.352 ± 0.323
1.555GluAsn: 1.555 ± 0.309
3.177GluPro: 3.177 ± 0.51
1.757GluGln: 1.757 ± 0.371
3.717GluArg: 3.717 ± 0.492
2.974GluSer: 2.974 ± 0.468
3.785GluThr: 3.785 ± 0.545
5.069GluVal: 5.069 ± 0.719
1.352GluTrp: 1.352 ± 0.306
1.419GluTyr: 1.419 ± 0.28
0.0GluXaa: 0.0 ± 0.0
Phe
2.095PheAla: 2.095 ± 0.304
0.27PheCys: 0.27 ± 0.176
2.095PheAsp: 2.095 ± 0.363
1.69PheGlu: 1.69 ± 0.388
0.135PhePhe: 0.135 ± 0.096
2.568PheGly: 2.568 ± 0.461
0.879PheHis: 0.879 ± 0.221
1.014PheIle: 1.014 ± 0.279
1.081PheLys: 1.081 ± 0.223
1.96PheLeu: 1.96 ± 0.469
0.608PheMet: 0.608 ± 0.187
0.608PheAsn: 0.608 ± 0.222
1.487PhePro: 1.487 ± 0.333
0.879PheGln: 0.879 ± 0.243
1.96PheArg: 1.96 ± 0.452
0.676PheSer: 0.676 ± 0.187
1.622PheThr: 1.622 ± 0.378
1.622PheVal: 1.622 ± 0.331
0.068PheTrp: 0.068 ± 0.065
0.811PheTyr: 0.811 ± 0.262
0.0PheXaa: 0.0 ± 0.0
Gly
7.84GlyAla: 7.84 ± 1.468
0.338GlyCys: 0.338 ± 0.184
4.529GlyAsp: 4.529 ± 0.514
4.258GlyGlu: 4.258 ± 0.499
2.771GlyPhe: 2.771 ± 0.49
7.57GlyGly: 7.57 ± 0.966
1.69GlyHis: 1.69 ± 0.241
3.785GlyIle: 3.785 ± 0.53
3.717GlyLys: 3.717 ± 0.447
7.097GlyLeu: 7.097 ± 0.739
1.487GlyMet: 1.487 ± 0.338
3.109GlyAsn: 3.109 ± 0.56
4.191GlyPro: 4.191 ± 0.5
4.191GlyGln: 4.191 ± 0.759
6.962GlyArg: 6.962 ± 0.602
5.204GlySer: 5.204 ± 0.624
6.489GlyThr: 6.489 ± 0.761
6.894GlyVal: 6.894 ± 0.906
2.028GlyTrp: 2.028 ± 0.378
2.636GlyTyr: 2.636 ± 0.467
0.0GlyXaa: 0.0 ± 0.0
His
2.163HisAla: 2.163 ± 0.447
0.0HisCys: 0.0 ± 0.0
1.149HisAsp: 1.149 ± 0.251
0.946HisGlu: 0.946 ± 0.27
0.676HisPhe: 0.676 ± 0.217
2.23HisGly: 2.23 ± 0.488
1.081HisHis: 1.081 ± 0.274
0.676HisIle: 0.676 ± 0.209
0.608HisLys: 0.608 ± 0.165
2.095HisLeu: 2.095 ± 0.393
0.473HisMet: 0.473 ± 0.186
0.338HisAsn: 0.338 ± 0.163
2.23HisPro: 2.23 ± 0.468
0.946HisGln: 0.946 ± 0.22
1.352HisArg: 1.352 ± 0.369
0.473HisSer: 0.473 ± 0.195
0.946HisThr: 0.946 ± 0.206
1.081HisVal: 1.081 ± 0.317
0.27HisTrp: 0.27 ± 0.141
0.203HisTyr: 0.203 ± 0.114
0.0HisXaa: 0.0 ± 0.0
Ile
4.191IleAla: 4.191 ± 0.682
0.541IleCys: 0.541 ± 0.219
3.042IleAsp: 3.042 ± 0.445
1.014IleGlu: 1.014 ± 0.201
0.338IlePhe: 0.338 ± 0.153
4.258IleGly: 4.258 ± 0.625
1.149IleHis: 1.149 ± 0.261
1.419IleIle: 1.419 ± 0.276
1.96IleLys: 1.96 ± 0.43
3.177IleLeu: 3.177 ± 0.371
1.217IleMet: 1.217 ± 0.355
1.014IleAsn: 1.014 ± 0.27
3.717IlePro: 3.717 ± 0.508
1.487IleGln: 1.487 ± 0.359
3.92IleArg: 3.92 ± 0.568
2.298IleSer: 2.298 ± 0.456
2.771IleThr: 2.771 ± 0.425
3.109IleVal: 3.109 ± 0.341
1.081IleTrp: 1.081 ± 0.198
0.879IleTyr: 0.879 ± 0.225
0.0IleXaa: 0.0 ± 0.0
Lys
5.34LysAla: 5.34 ± 0.683
0.135LysCys: 0.135 ± 0.103
2.298LysAsp: 2.298 ± 0.385
2.298LysGlu: 2.298 ± 0.391
0.473LysPhe: 0.473 ± 0.193
2.501LysGly: 2.501 ± 0.502
0.676LysHis: 0.676 ± 0.228
1.825LysIle: 1.825 ± 0.333
0.406LysLys: 0.406 ± 0.182
2.906LysLeu: 2.906 ± 0.535
1.014LysMet: 1.014 ± 0.25
0.676LysAsn: 0.676 ± 0.287
2.095LysPro: 2.095 ± 0.367
0.406LysGln: 0.406 ± 0.133
2.163LysArg: 2.163 ± 0.357
2.433LysSer: 2.433 ± 0.417
2.163LysThr: 2.163 ± 0.46
3.785LysVal: 3.785 ± 0.692
0.946LysTrp: 0.946 ± 0.251
0.541LysTyr: 0.541 ± 0.18
0.0LysXaa: 0.0 ± 0.0
Leu
8.922LeuAla: 8.922 ± 0.878
0.743LeuCys: 0.743 ± 0.247
5.88LeuAsp: 5.88 ± 0.654
4.934LeuGlu: 4.934 ± 0.612
1.757LeuPhe: 1.757 ± 0.336
7.84LeuGly: 7.84 ± 0.732
1.555LeuHis: 1.555 ± 0.373
2.974LeuIle: 2.974 ± 0.51
3.785LeuLys: 3.785 ± 0.612
5.204LeuLeu: 5.204 ± 0.664
0.879LeuMet: 0.879 ± 0.23
2.163LeuAsn: 2.163 ± 0.39
4.055LeuPro: 4.055 ± 0.719
3.042LeuGln: 3.042 ± 0.507
6.016LeuArg: 6.016 ± 0.766
3.042LeuSer: 3.042 ± 0.43
6.016LeuThr: 6.016 ± 0.648
5.204LeuVal: 5.204 ± 0.532
1.284LeuTrp: 1.284 ± 0.29
2.095LeuTyr: 2.095 ± 0.332
0.0LeuXaa: 0.0 ± 0.0
Met
3.244MetAla: 3.244 ± 0.397
0.338MetCys: 0.338 ± 0.157
1.96MetAsp: 1.96 ± 0.345
1.757MetGlu: 1.757 ± 0.299
0.541MetPhe: 0.541 ± 0.171
2.298MetGly: 2.298 ± 0.399
0.135MetHis: 0.135 ± 0.133
1.014MetIle: 1.014 ± 0.251
0.473MetLys: 0.473 ± 0.204
1.555MetLeu: 1.555 ± 0.342
0.473MetMet: 0.473 ± 0.174
0.406MetAsn: 0.406 ± 0.183
1.217MetPro: 1.217 ± 0.337
0.473MetGln: 0.473 ± 0.178
1.284MetArg: 1.284 ± 0.306
1.757MetSer: 1.757 ± 0.366
3.92MetThr: 3.92 ± 0.535
1.284MetVal: 1.284 ± 0.311
0.135MetTrp: 0.135 ± 0.101
0.406MetTyr: 0.406 ± 0.202
0.0MetXaa: 0.0 ± 0.0
Asn
3.785AsnAla: 3.785 ± 0.562
0.338AsnCys: 0.338 ± 0.127
1.217AsnAsp: 1.217 ± 0.369
1.149AsnGlu: 1.149 ± 0.235
0.608AsnPhe: 0.608 ± 0.26
2.974AsnGly: 2.974 ± 0.504
0.473AsnHis: 0.473 ± 0.195
1.014AsnIle: 1.014 ± 0.257
1.217AsnLys: 1.217 ± 0.342
2.433AsnLeu: 2.433 ± 0.292
0.946AsnMet: 0.946 ± 0.271
1.081AsnAsn: 1.081 ± 0.27
3.244AsnPro: 3.244 ± 0.545
0.541AsnGln: 0.541 ± 0.197
1.757AsnArg: 1.757 ± 0.309
1.217AsnSer: 1.217 ± 0.251
2.636AsnThr: 2.636 ± 0.426
2.095AsnVal: 2.095 ± 0.355
0.338AsnTrp: 0.338 ± 0.145
0.879AsnTyr: 0.879 ± 0.184
0.0AsnXaa: 0.0 ± 0.0
Pro
8.314ProAla: 8.314 ± 0.757
0.541ProCys: 0.541 ± 0.202
5.475ProAsp: 5.475 ± 0.863
4.596ProGlu: 4.596 ± 0.768
1.96ProPhe: 1.96 ± 0.396
4.799ProGly: 4.799 ± 0.502
1.555ProHis: 1.555 ± 0.296
3.312ProIle: 3.312 ± 0.457
1.757ProLys: 1.757 ± 0.387
3.244ProLeu: 3.244 ± 0.602
1.014ProMet: 1.014 ± 0.254
2.163ProAsn: 2.163 ± 0.408
3.65ProPro: 3.65 ± 0.528
1.757ProGln: 1.757 ± 0.658
3.785ProArg: 3.785 ± 0.681
3.988ProSer: 3.988 ± 0.65
4.664ProThr: 4.664 ± 0.718
4.055ProVal: 4.055 ± 0.494
1.487ProTrp: 1.487 ± 0.341
1.217ProTyr: 1.217 ± 0.307
0.0ProXaa: 0.0 ± 0.0
Gln
4.461GlnAla: 4.461 ± 0.795
0.0GlnCys: 0.0 ± 0.0
1.487GlnAsp: 1.487 ± 0.282
1.96GlnGlu: 1.96 ± 0.338
1.352GlnPhe: 1.352 ± 0.256
3.109GlnGly: 3.109 ± 0.632
0.541GlnHis: 0.541 ± 0.212
2.568GlnIle: 2.568 ± 0.376
0.541GlnLys: 0.541 ± 0.18
2.366GlnLeu: 2.366 ± 0.394
1.284GlnMet: 1.284 ± 0.29
1.217GlnAsn: 1.217 ± 0.341
1.555GlnPro: 1.555 ± 0.51
1.69GlnGln: 1.69 ± 0.859
2.501GlnArg: 2.501 ± 0.349
1.487GlnSer: 1.487 ± 0.325
1.487GlnThr: 1.487 ± 0.341
2.568GlnVal: 2.568 ± 0.4
0.811GlnTrp: 0.811 ± 0.291
0.743GlnTyr: 0.743 ± 0.265
0.0GlnXaa: 0.0 ± 0.0
Arg
8.246ArgAla: 8.246 ± 0.849
0.608ArgCys: 0.608 ± 0.204
4.596ArgAsp: 4.596 ± 0.601
3.582ArgGlu: 3.582 ± 0.621
1.487ArgPhe: 1.487 ± 0.274
5.948ArgGly: 5.948 ± 0.597
0.879ArgHis: 0.879 ± 0.249
2.568ArgIle: 2.568 ± 0.371
2.433ArgLys: 2.433 ± 0.399
4.799ArgLeu: 4.799 ± 0.513
2.906ArgMet: 2.906 ± 0.549
2.501ArgAsn: 2.501 ± 0.474
4.799ArgPro: 4.799 ± 0.573
1.825ArgGln: 1.825 ± 0.343
6.016ArgArg: 6.016 ± 1.035
3.447ArgSer: 3.447 ± 0.481
4.258ArgThr: 4.258 ± 0.673
5.542ArgVal: 5.542 ± 0.569
1.352ArgTrp: 1.352 ± 0.296
2.028ArgTyr: 2.028 ± 0.365
0.0ArgXaa: 0.0 ± 0.0
Ser
6.421SerAla: 6.421 ± 0.748
0.338SerCys: 0.338 ± 0.156
3.38SerAsp: 3.38 ± 0.44
2.704SerGlu: 2.704 ± 0.499
1.014SerPhe: 1.014 ± 0.226
5.137SerGly: 5.137 ± 0.792
0.879SerHis: 0.879 ± 0.24
2.298SerIle: 2.298 ± 0.355
2.028SerLys: 2.028 ± 0.411
3.785SerLeu: 3.785 ± 0.49
1.352SerMet: 1.352 ± 0.328
1.217SerAsn: 1.217 ± 0.261
2.974SerPro: 2.974 ± 0.415
1.69SerGln: 1.69 ± 0.264
3.853SerArg: 3.853 ± 0.485
3.042SerSer: 3.042 ± 0.502
3.177SerThr: 3.177 ± 0.351
3.38SerVal: 3.38 ± 0.579
0.879SerTrp: 0.879 ± 0.219
2.163SerTyr: 2.163 ± 0.321
0.0SerXaa: 0.0 ± 0.0
Thr
10.003ThrAla: 10.003 ± 0.81
0.541ThrCys: 0.541 ± 0.183
3.65ThrAsp: 3.65 ± 0.479
3.447ThrGlu: 3.447 ± 0.479
1.757ThrPhe: 1.757 ± 0.307
6.218ThrGly: 6.218 ± 0.937
1.352ThrHis: 1.352 ± 0.299
2.839ThrIle: 2.839 ± 0.496
2.501ThrLys: 2.501 ± 0.318
6.489ThrLeu: 6.489 ± 0.816
1.622ThrMet: 1.622 ± 0.376
2.298ThrAsn: 2.298 ± 0.417
4.461ThrPro: 4.461 ± 0.72
1.555ThrGln: 1.555 ± 0.391
3.92ThrArg: 3.92 ± 0.426
4.258ThrSer: 4.258 ± 0.609
6.353ThrThr: 6.353 ± 0.75
6.151ThrVal: 6.151 ± 0.689
0.811ThrTrp: 0.811 ± 0.251
1.757ThrTyr: 1.757 ± 0.387
0.0ThrXaa: 0.0 ± 0.0
Val
8.381ValAla: 8.381 ± 0.689
0.608ValCys: 0.608 ± 0.24
4.326ValAsp: 4.326 ± 0.526
3.853ValGlu: 3.853 ± 0.526
2.704ValPhe: 2.704 ± 0.34
6.151ValGly: 6.151 ± 0.835
1.622ValHis: 1.622 ± 0.389
3.177ValIle: 3.177 ± 0.468
3.042ValLys: 3.042 ± 0.414
6.286ValLeu: 6.286 ± 0.655
2.163ValMet: 2.163 ± 0.366
1.419ValAsn: 1.419 ± 0.3
5.002ValPro: 5.002 ± 0.535
3.177ValGln: 3.177 ± 0.427
5.34ValArg: 5.34 ± 0.643
4.461ValSer: 4.461 ± 0.539
5.678ValThr: 5.678 ± 0.602
6.827ValVal: 6.827 ± 0.745
1.149ValTrp: 1.149 ± 0.277
1.622ValTyr: 1.622 ± 0.41
0.0ValXaa: 0.0 ± 0.0
Trp
1.555TrpAla: 1.555 ± 0.366
0.135TrpCys: 0.135 ± 0.088
1.284TrpAsp: 1.284 ± 0.274
1.622TrpGlu: 1.622 ± 0.301
0.676TrpPhe: 0.676 ± 0.299
1.352TrpGly: 1.352 ± 0.321
0.27TrpHis: 0.27 ± 0.139
0.879TrpIle: 0.879 ± 0.285
0.406TrpLys: 0.406 ± 0.194
1.555TrpLeu: 1.555 ± 0.287
0.676TrpMet: 0.676 ± 0.241
1.014TrpAsn: 1.014 ± 0.26
1.419TrpPro: 1.419 ± 0.31
0.811TrpGln: 0.811 ± 0.195
1.014TrpArg: 1.014 ± 0.252
0.946TrpSer: 0.946 ± 0.207
1.419TrpThr: 1.419 ± 0.301
1.893TrpVal: 1.893 ± 0.428
0.608TrpTrp: 0.608 ± 0.243
0.338TrpTyr: 0.338 ± 0.179
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.839TyrAla: 2.839 ± 0.406
0.068TyrCys: 0.068 ± 0.073
1.217TyrAsp: 1.217 ± 0.307
1.96TyrGlu: 1.96 ± 0.473
0.676TyrPhe: 0.676 ± 0.173
2.568TyrGly: 2.568 ± 0.422
0.203TyrHis: 0.203 ± 0.113
1.081TyrIle: 1.081 ± 0.286
1.149TyrLys: 1.149 ± 0.349
2.433TyrLeu: 2.433 ± 0.433
0.338TyrMet: 0.338 ± 0.159
0.743TyrAsn: 0.743 ± 0.286
1.893TyrPro: 1.893 ± 0.378
0.743TyrGln: 0.743 ± 0.204
2.568TyrArg: 2.568 ± 0.376
0.811TyrSer: 0.811 ± 0.22
1.419TyrThr: 1.419 ± 0.313
1.757TyrVal: 1.757 ± 0.314
0.27TyrTrp: 0.27 ± 0.133
0.203TyrTyr: 0.203 ± 0.112
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (14796 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski