Amino acid dipepetide frequency for Methylomonas methanica (strain MC09)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.222AlaAla: 10.222 ± 0.151	1.138AlaCys: 1.138 ± 0.027	6.022AlaAsp: 6.022 ± 0.069	6.814AlaGlu: 6.814 ± 0.082	3.644AlaPhe: 3.644 ± 0.045	7.424AlaGly: 7.424 ± 0.089	1.905AlaHis: 1.905 ± 0.037	5.816AlaIle: 5.816 ± 0.066	4.377AlaLys: 4.377 ± 0.059	10.896AlaLeu: 10.896 ± 0.125	2.589AlaMet: 2.589 ± 0.046	3.476AlaAsn: 3.476 ± 0.049	3.181AlaPro: 3.181 ± 0.056	3.891AlaGln: 3.891 ± 0.058	4.565AlaArg: 4.565 ± 0.064	5.095AlaSer: 5.095 ± 0.067	4.347AlaThr: 4.347 ± 0.064	6.757AlaVal: 6.757 ± 0.085	1.287AlaTrp: 1.287 ± 0.035	2.656AlaTyr: 2.656 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	0.901CysAla: 0.901 ± 0.027	0.181CysCys: 0.181 ± 0.011	0.581CysAsp: 0.581 ± 0.02	0.544CysGlu: 0.544 ± 0.022	0.431CysPhe: 0.431 ± 0.019	0.939CysGly: 0.939 ± 0.029	0.345CysHis: 0.345 ± 0.018	0.523CysIle: 0.523 ± 0.019	0.413CysLys: 0.413 ± 0.016	1.236CysLeu: 1.236 ± 0.029	0.214CysMet: 0.214 ± 0.012	0.351CysAsn: 0.351 ± 0.016	0.511CysPro: 0.511 ± 0.02	0.41CysGln: 0.41 ± 0.017	0.735CysArg: 0.735 ± 0.024	0.703CysSer: 0.703 ± 0.022	0.454CysThr: 0.454 ± 0.019	0.686CysVal: 0.686 ± 0.023	0.147CysTrp: 0.147 ± 0.01	0.36CysTyr: 0.36 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	5.311AspAla: 5.311 ± 0.076	0.649AspCys: 0.649 ± 0.022	3.127AspAsp: 3.127 ± 0.051	3.369AspGlu: 3.369 ± 0.056	2.596AspPhe: 2.596 ± 0.043	3.888AspGly: 3.888 ± 0.066	1.133AspHis: 1.133 ± 0.025	3.793AspIle: 3.793 ± 0.052	2.879AspLys: 2.879 ± 0.051	5.652AspLeu: 5.652 ± 0.068	1.314AspMet: 1.314 ± 0.032	2.242AspAsn: 2.242 ± 0.042	2.471AspPro: 2.471 ± 0.044	2.284AspGln: 2.284 ± 0.059	2.86AspArg: 2.86 ± 0.042	3.329AspSer: 3.329 ± 0.05	2.772AspThr: 2.772 ± 0.051	3.532AspVal: 3.532 ± 0.05	1.007AspTrp: 1.007 ± 0.026	2.083AspTyr: 2.083 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.48GluAla: 5.48 ± 0.064	0.479GluCys: 0.479 ± 0.018	2.561GluAsp: 2.561 ± 0.047	2.829GluGlu: 2.829 ± 0.052	2.287GluPhe: 2.287 ± 0.041	3.015GluGly: 3.015 ± 0.052	1.521GluHis: 1.521 ± 0.033	3.836GluIle: 3.836 ± 0.056	3.29GluLys: 3.29 ± 0.07	6.498GluLeu: 6.498 ± 0.073	1.471GluMet: 1.471 ± 0.033	2.429GluAsn: 2.429 ± 0.046	2.139GluPro: 2.139 ± 0.037	3.791GluGln: 3.791 ± 0.061	3.454GluArg: 3.454 ± 0.051	3.141GluSer: 3.141 ± 0.043	3.301GluThr: 3.301 ± 0.045	3.551GluVal: 3.551 ± 0.052	0.741GluTrp: 0.741 ± 0.024	1.634GluTyr: 1.634 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	3.792PheAla: 3.792 ± 0.052	0.54PheCys: 0.54 ± 0.02	2.707PheAsp: 2.707 ± 0.041	2.333PheGlu: 2.333 ± 0.045	1.848PhePhe: 1.848 ± 0.038	3.196PheGly: 3.196 ± 0.061	0.789PheHis: 0.789 ± 0.022	2.376PheIle: 2.376 ± 0.042	1.881PheLys: 1.881 ± 0.04	3.819PheLeu: 3.819 ± 0.057	0.953PheMet: 0.953 ± 0.026	1.898PheAsn: 1.898 ± 0.043	1.575PhePro: 1.575 ± 0.039	1.456PheGln: 1.456 ± 0.029	1.92PheArg: 1.92 ± 0.037	3.16PheSer: 3.16 ± 0.054	2.081PheThr: 2.081 ± 0.038	2.715PheVal: 2.715 ± 0.048	0.583PheTrp: 0.583 ± 0.021	1.359PheTyr: 1.359 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	5.295GlyAla: 5.295 ± 0.07	0.933GlyCys: 0.933 ± 0.027	3.692GlyAsp: 3.692 ± 0.057	3.987GlyGlu: 3.987 ± 0.061	3.54GlyPhe: 3.54 ± 0.055	5.104GlyGly: 5.104 ± 0.077	1.611GlyHis: 1.611 ± 0.039	4.525GlyIle: 4.525 ± 0.055	3.855GlyLys: 3.855 ± 0.055	7.744GlyLeu: 7.744 ± 0.076	1.942GlyMet: 1.942 ± 0.046	2.799GlyAsn: 2.799 ± 0.052	1.913GlyPro: 1.913 ± 0.041	3.082GlyGln: 3.082 ± 0.047	3.803GlyArg: 3.803 ± 0.055	4.289GlySer: 4.289 ± 0.064	3.106GlyThr: 3.106 ± 0.057	4.964GlyVal: 4.964 ± 0.057	1.094GlyTrp: 1.094 ± 0.027	2.57GlyTyr: 2.57 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.096HisAla: 2.096 ± 0.039	0.33HisCys: 0.33 ± 0.016	1.266HisAsp: 1.266 ± 0.034	1.174HisGlu: 1.174 ± 0.028	1.086HisPhe: 1.086 ± 0.026	1.681HisGly: 1.681 ± 0.037	0.722HisHis: 0.722 ± 0.027	1.375HisIle: 1.375 ± 0.031	0.961HisLys: 0.961 ± 0.024	2.352HisLeu: 2.352 ± 0.035	0.492HisMet: 0.492 ± 0.019	0.836HisAsn: 0.836 ± 0.026	1.299HisPro: 1.299 ± 0.034	0.998HisGln: 0.998 ± 0.028	1.33HisArg: 1.33 ± 0.032	1.397HisSer: 1.397 ± 0.03	1.099HisThr: 1.099 ± 0.027	1.276HisVal: 1.276 ± 0.028	0.426HisTrp: 0.426 ± 0.019	0.934HisTyr: 0.934 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.283IleAla: 6.283 ± 0.076	0.644IleCys: 0.644 ± 0.024	4.166IleAsp: 4.166 ± 0.057	4.016IleGlu: 4.016 ± 0.064	2.019IlePhe: 2.019 ± 0.036	4.477IleGly: 4.477 ± 0.065	1.321IleHis: 1.321 ± 0.031	3.075IleIle: 3.075 ± 0.055	3.022IleLys: 3.022 ± 0.049	5.403IleLeu: 5.403 ± 0.062	1.13IleMet: 1.13 ± 0.021	2.686IleAsn: 2.686 ± 0.047	2.715IlePro: 2.715 ± 0.043	2.283IleGln: 2.283 ± 0.038	3.171IleArg: 3.171 ± 0.049	3.941IleSer: 3.941 ± 0.053	3.062IleThr: 3.062 ± 0.053	3.966IleVal: 3.966 ± 0.054	0.619IleTrp: 0.619 ± 0.022	1.653IleTyr: 1.653 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	4.517LysAla: 4.517 ± 0.066	0.338LysCys: 0.338 ± 0.014	2.254LysAsp: 2.254 ± 0.049	2.19LysGlu: 2.19 ± 0.044	1.354LysPhe: 1.354 ± 0.034	2.668LysGly: 2.668 ± 0.049	1.198LysHis: 1.198 ± 0.031	3.007LysIle: 3.007 ± 0.048	2.376LysLys: 2.376 ± 0.048	5.154LysLeu: 5.154 ± 0.056	1.073LysMet: 1.073 ± 0.028	1.994LysAsn: 1.994 ± 0.039	2.511LysPro: 2.511 ± 0.046	2.906LysGln: 2.906 ± 0.056	2.587LysArg: 2.587 ± 0.042	2.713LysSer: 2.713 ± 0.044	2.962LysThr: 2.962 ± 0.05	2.919LysVal: 2.919 ± 0.049	0.478LysTrp: 0.478 ± 0.018	1.168LysTyr: 1.168 ± 0.033	0.0LysXaa: 0.0 ± 0.0
Leu	11.508LeuAla: 11.508 ± 0.122	1.125LeuCys: 1.125 ± 0.032	6.009LeuAsp: 6.009 ± 0.073	6.073LeuGlu: 6.073 ± 0.074	4.524LeuPhe: 4.524 ± 0.075	7.254LeuGly: 7.254 ± 0.079	2.439LeuHis: 2.439 ± 0.045	6.283LeuIle: 6.283 ± 0.076	5.309LeuLys: 5.309 ± 0.07	12.566LeuLeu: 12.566 ± 0.151	2.453LeuMet: 2.453 ± 0.047	4.543LeuAsn: 4.543 ± 0.056	5.576LeuPro: 5.576 ± 0.082	5.225LeuGln: 5.225 ± 0.072	5.819LeuArg: 5.819 ± 0.071	7.538LeuSer: 7.538 ± 0.081	5.932LeuThr: 5.932 ± 0.084	6.367LeuVal: 6.367 ± 0.073	1.215LeuTrp: 1.215 ± 0.03	2.744LeuTyr: 2.744 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	2.513MetAla: 2.513 ± 0.044	0.155MetCys: 0.155 ± 0.01	1.189MetAsp: 1.189 ± 0.028	1.157MetGlu: 1.157 ± 0.029	0.662MetPhe: 0.662 ± 0.02	1.531MetGly: 1.531 ± 0.037	0.519MetHis: 0.519 ± 0.022	1.253MetIle: 1.253 ± 0.03	1.152MetLys: 1.152 ± 0.03	2.693MetLeu: 2.693 ± 0.045	0.59MetMet: 0.59 ± 0.023	1.009MetAsn: 1.009 ± 0.028	1.374MetPro: 1.374 ± 0.026	1.194MetGln: 1.194 ± 0.029	1.332MetArg: 1.332 ± 0.029	1.519MetSer: 1.519 ± 0.033	1.421MetThr: 1.421 ± 0.029	1.396MetVal: 1.396 ± 0.03	0.16MetTrp: 0.16 ± 0.01	0.418MetTyr: 0.418 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.712AsnAla: 3.712 ± 0.057	0.437AsnCys: 0.437 ± 0.017	2.137AsnAsp: 2.137 ± 0.041	1.813AsnGlu: 1.813 ± 0.04	1.453AsnPhe: 1.453 ± 0.031	2.938AsnGly: 2.938 ± 0.053	0.939AsnHis: 0.939 ± 0.028	2.43AsnIle: 2.43 ± 0.047	1.661AsnLys: 1.661 ± 0.039	4.248AsnLeu: 4.248 ± 0.053	0.838AsnMet: 0.838 ± 0.025	1.657AsnAsn: 1.657 ± 0.044	2.447AsnPro: 2.447 ± 0.042	2.034AsnGln: 2.034 ± 0.04	2.469AsnArg: 2.469 ± 0.036	2.281AsnSer: 2.281 ± 0.041	1.998AsnThr: 1.998 ± 0.041	2.333AsnVal: 2.333 ± 0.042	0.625AsnTrp: 0.625 ± 0.023	1.198AsnTyr: 1.198 ± 0.033	0.0AsnXaa: 0.0 ± 0.0
Pro	4.409ProAla: 4.409 ± 0.068	0.376ProCys: 0.376 ± 0.017	3.051ProAsp: 3.051 ± 0.054	3.338ProGlu: 3.338 ± 0.05	1.782ProPhe: 1.782 ± 0.037	3.258ProGly: 3.258 ± 0.052	0.949ProHis: 0.949 ± 0.024	2.434ProIle: 2.434 ± 0.041	1.826ProLys: 1.826 ± 0.04	4.626ProLeu: 4.626 ± 0.062	1.009ProMet: 1.009 ± 0.026	1.674ProAsn: 1.674 ± 0.036	1.786ProPro: 1.786 ± 0.045	1.817ProGln: 1.817 ± 0.035	1.905ProArg: 1.905 ± 0.038	2.428ProSer: 2.428 ± 0.044	2.074ProThr: 2.074 ± 0.039	3.483ProVal: 3.483 ± 0.054	0.554ProTrp: 0.554 ± 0.022	1.218ProTyr: 1.218 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	5.421GlnAla: 5.421 ± 0.077	0.407GlnCys: 0.407 ± 0.016	2.146GlnAsp: 2.146 ± 0.039	2.155GlnGlu: 2.155 ± 0.044	1.796GlnPhe: 1.796 ± 0.036	2.869GlnGly: 2.869 ± 0.046	1.288GlnHis: 1.288 ± 0.03	2.774GlnIle: 2.774 ± 0.059	1.975GlnLys: 1.975 ± 0.043	5.337GlnLeu: 5.337 ± 0.079	0.979GlnMet: 0.979 ± 0.026	1.783GlnAsn: 1.783 ± 0.041	2.266GlnPro: 2.266 ± 0.039	3.329GlnGln: 3.329 ± 0.067	3.016GlnArg: 3.016 ± 0.051	2.625GlnSer: 2.625 ± 0.048	2.698GlnThr: 2.698 ± 0.042	3.031GlnVal: 3.031 ± 0.048	0.649GlnTrp: 0.649 ± 0.023	1.323GlnTyr: 1.323 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	4.059ArgAla: 4.059 ± 0.052	0.563ArgCys: 0.563 ± 0.022	2.886ArgAsp: 2.886 ± 0.043	3.228ArgGlu: 3.228 ± 0.051	2.696ArgPhe: 2.696 ± 0.048	3.077ArgGly: 3.077 ± 0.05	1.584ArgHis: 1.584 ± 0.033	3.675ArgIle: 3.675 ± 0.051	2.471ArgLys: 2.471 ± 0.046	6.52ArgLeu: 6.52 ± 0.085	1.365ArgMet: 1.365 ± 0.031	2.236ArgAsn: 2.236 ± 0.037	2.146ArgPro: 2.146 ± 0.042	3.311ArgGln: 3.311 ± 0.051	3.352ArgArg: 3.352 ± 0.053	2.81ArgSer: 2.81 ± 0.039	2.219ArgThr: 2.219 ± 0.036	3.458ArgVal: 3.458 ± 0.053	0.893ArgTrp: 0.893 ± 0.023	2.048ArgTyr: 2.048 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	5.732SerAla: 5.732 ± 0.07	0.63SerCys: 0.63 ± 0.021	3.173SerAsp: 3.173 ± 0.05	3.501SerGlu: 3.501 ± 0.05	2.522SerPhe: 2.522 ± 0.041	5.155SerGly: 5.155 ± 0.076	1.422SerHis: 1.422 ± 0.034	3.594SerIle: 3.594 ± 0.048	2.496SerLys: 2.496 ± 0.043	6.765SerLeu: 6.765 ± 0.077	1.37SerMet: 1.37 ± 0.033	2.256SerAsn: 2.256 ± 0.043	2.548SerPro: 2.548 ± 0.045	2.648SerGln: 2.648 ± 0.046	3.373SerArg: 3.373 ± 0.055	3.692SerSer: 3.692 ± 0.068	2.815SerThr: 2.815 ± 0.053	4.278SerVal: 4.278 ± 0.056	0.805SerTrp: 0.805 ± 0.023	1.736SerTyr: 1.736 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.043ThrAla: 5.043 ± 0.067	0.436ThrCys: 0.436 ± 0.02	3.018ThrAsp: 3.018 ± 0.057	2.893ThrGlu: 2.893 ± 0.048	1.828ThrPhe: 1.828 ± 0.036	4.181ThrGly: 4.181 ± 0.054	1.145ThrHis: 1.145 ± 0.027	2.706ThrIle: 2.706 ± 0.041	1.652ThrLys: 1.652 ± 0.037	6.359ThrLeu: 6.359 ± 0.077	0.939ThrMet: 0.939 ± 0.023	1.565ThrAsn: 1.565 ± 0.036	2.849ThrPro: 2.849 ± 0.046	2.2ThrGln: 2.2 ± 0.044	2.502ThrArg: 2.502 ± 0.044	2.703ThrSer: 2.703 ± 0.051	2.572ThrThr: 2.572 ± 0.072	3.851ThrVal: 3.851 ± 0.05	0.557ThrTrp: 0.557 ± 0.021	1.31ThrTyr: 1.31 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	6.316ValAla: 6.316 ± 0.073	0.756ValCys: 0.756 ± 0.022	3.91ValAsp: 3.91 ± 0.059	4.057ValGlu: 4.057 ± 0.056	2.922ValPhe: 2.922 ± 0.049	4.353ValGly: 4.353 ± 0.061	1.229ValHis: 1.229 ± 0.027	4.112ValIle: 4.112 ± 0.051	3.147ValLys: 3.147 ± 0.054	7.044ValLeu: 7.044 ± 0.078	1.682ValMet: 1.682 ± 0.035	2.687ValAsn: 2.687 ± 0.044	2.654ValPro: 2.654 ± 0.045	2.285ValGln: 2.285 ± 0.043	3.266ValArg: 3.266 ± 0.049	4.483ValSer: 4.483 ± 0.056	3.511ValThr: 3.511 ± 0.051	4.67ValVal: 4.67 ± 0.068	0.86ValTrp: 0.86 ± 0.026	1.873ValTyr: 1.873 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	0.899TrpAla: 0.899 ± 0.028	0.143TrpCys: 0.143 ± 0.011	0.636TrpAsp: 0.636 ± 0.021	0.605TrpGlu: 0.605 ± 0.021	0.585TrpPhe: 0.585 ± 0.02	0.753TrpGly: 0.753 ± 0.024	0.381TrpHis: 0.381 ± 0.014	0.684TrpIle: 0.684 ± 0.021	0.48TrpLys: 0.48 ± 0.019	2.122TrpLeu: 2.122 ± 0.044	0.324TrpMet: 0.324 ± 0.016	0.518TrpAsn: 0.518 ± 0.021	0.587TrpPro: 0.587 ± 0.019	0.986TrpGln: 0.986 ± 0.033	0.945TrpArg: 0.945 ± 0.028	0.768TrpSer: 0.768 ± 0.025	0.542TrpThr: 0.542 ± 0.022	0.886TrpVal: 0.886 ± 0.024	0.193TrpTrp: 0.193 ± 0.012	0.341TrpTyr: 0.341 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.59TyrAla: 2.59 ± 0.044	0.407TyrCys: 0.407 ± 0.02	1.756TyrAsp: 1.756 ± 0.071	1.372TyrGlu: 1.372 ± 0.031	1.406TyrPhe: 1.406 ± 0.035	2.087TyrGly: 2.087 ± 0.041	0.688TyrHis: 0.688 ± 0.023	1.381TyrIle: 1.381 ± 0.032	1.145TyrLys: 1.145 ± 0.03	3.467TyrLeu: 3.467 ± 0.051	0.508TyrMet: 0.508 ± 0.019	1.009TyrAsn: 1.009 ± 0.031	1.45TyrPro: 1.45 ± 0.037	1.738TyrGln: 1.738 ± 0.037	2.195TyrArg: 2.195 ± 0.042	1.898TyrSer: 1.898 ± 0.04	1.343TyrThr: 1.343 ± 0.035	1.699TyrVal: 1.699 ± 0.039	0.486TyrTrp: 0.486 ± 0.019	1.02TyrTyr: 1.02 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4435 proteins (1455403 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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