Actinobaculum massiliense ACS-171-V-Col2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinobaculum; Actinobaculum massiliense

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1696 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9ED07|K9ED07_9ACTO Site-specific DNA-methyltransferase (adenine-specific) OS=Actinobaculum massiliense ACS-171-V-Col2 OX=883066 GN=HMPREF9233_00905 PE=4 SV=1
MM1 pKa = 7.99GDD3 pKa = 3.38TFLEE7 pKa = 4.29SLGYY11 pKa = 10.68LPDD14 pKa = 3.29WEE16 pKa = 4.83KK17 pKa = 11.17NVEE20 pKa = 3.95AFPWYY25 pKa = 10.1EE26 pKa = 4.06MPSLPNYY33 pKa = 9.76RR34 pKa = 11.84QFDD37 pKa = 4.31HH38 pKa = 6.87ISVYY42 pKa = 10.53RR43 pKa = 11.84DD44 pKa = 3.12PSGSNICLYY53 pKa = 11.2GMVGGGLGEE62 pKa = 4.19SVVVFGEE69 pKa = 4.11SCVNVSAWQVTPGLAEE85 pKa = 3.95VSILDD90 pKa = 3.91AGGEE94 pKa = 4.31VMNKK98 pKa = 9.42LLAMVDD104 pKa = 4.06DD105 pKa = 3.98PHH107 pKa = 7.84LYY109 pKa = 9.95PMYY112 pKa = 9.54PASEE116 pKa = 4.02VGEE119 pKa = 4.28PVFHH123 pKa = 6.59TNYY126 pKa = 10.48RR127 pKa = 11.84MGAIAVDD134 pKa = 3.66VEE136 pKa = 4.43VFDD139 pKa = 6.71DD140 pKa = 3.77VALWKK145 pKa = 10.28AQQKK149 pKa = 9.43PLQIDD154 pKa = 4.1GKK156 pKa = 10.32RR157 pKa = 11.84AADD160 pKa = 3.49ADD162 pKa = 3.97KK163 pKa = 11.12LRR165 pKa = 11.84EE166 pKa = 3.98QTDD169 pKa = 3.53DD170 pKa = 3.69SVTGGSGEE178 pKa = 4.12EE179 pKa = 4.85LYY181 pKa = 10.91LGPQFATSPWLFEE194 pKa = 4.26FYY196 pKa = 10.61AGEE199 pKa = 4.02AAAEE203 pKa = 4.1DD204 pKa = 4.27LSAISAVCAVCYY216 pKa = 10.1QVDD219 pKa = 3.64IEE221 pKa = 4.57TNALTGRR228 pKa = 11.84PWYY231 pKa = 10.19KK232 pKa = 10.09IEE234 pKa = 4.55ADD236 pKa = 3.75CGFPITIGLPINSHH250 pKa = 6.52PAPMPGSVIDD260 pKa = 3.83GKK262 pKa = 10.0MFITGTTGFWLPEE275 pKa = 4.08NLDD278 pKa = 3.63SS279 pKa = 5.34

Molecular weight: 30.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9EEK8|K9EEK8_9ACTO Uncharacterized protein OS=Actinobaculum massiliense ACS-171-V-Col2 OX=883066 GN=HMPREF9233_00461 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.31LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1696

0

1696

593390

29

3008

349.9

37.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.023 ± 0.076

0.684 ± 0.016

5.686 ± 0.054

6.86 ± 0.071

3.297 ± 0.038

8.649 ± 0.051

1.88 ± 0.022

5.195 ± 0.05

3.34 ± 0.041

9.224 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.074 ± 0.028

2.936 ± 0.033

4.968 ± 0.044

3.288 ± 0.037

6.617 ± 0.061

6.096 ± 0.042

5.599 ± 0.047

7.845 ± 0.049

1.365 ± 0.021

2.376 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski