Amino acid dipepetide frequency for Microbacterium lemovicicum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.554AlaAla: 22.554 ± 0.231
0.665AlaCys: 0.665 ± 0.03
9.243AlaAsp: 9.243 ± 0.114
7.888AlaGlu: 7.888 ± 0.105
3.925AlaPhe: 3.925 ± 0.07
12.89AlaGly: 12.89 ± 0.121
2.484AlaHis: 2.484 ± 0.052
5.517AlaIle: 5.517 ± 0.08
2.27AlaLys: 2.27 ± 0.056
14.237AlaLeu: 14.237 ± 0.128
2.631AlaMet: 2.631 ± 0.048
1.985AlaAsn: 1.985 ± 0.046
7.17AlaPro: 7.17 ± 0.108
3.831AlaGln: 3.831 ± 0.062
9.654AlaArg: 9.654 ± 0.122
7.641AlaSer: 7.641 ± 0.093
7.542AlaThr: 7.542 ± 0.099
12.435AlaVal: 12.435 ± 0.122
1.994AlaTrp: 1.994 ± 0.051
2.421AlaTyr: 2.421 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.648CysAla: 0.648 ± 0.026
0.042CysCys: 0.042 ± 0.007
0.319CysAsp: 0.319 ± 0.017
0.224CysGlu: 0.224 ± 0.015
0.16CysPhe: 0.16 ± 0.014
0.517CysGly: 0.517 ± 0.024
0.093CysHis: 0.093 ± 0.01
0.181CysIle: 0.181 ± 0.014
0.047CysLys: 0.047 ± 0.007
0.41CysLeu: 0.41 ± 0.02
0.065CysMet: 0.065 ± 0.008
0.077CysAsn: 0.077 ± 0.01
0.282CysPro: 0.282 ± 0.017
0.107CysGln: 0.107 ± 0.011
0.329CysArg: 0.329 ± 0.018
0.297CysSer: 0.297 ± 0.018
0.299CysThr: 0.299 ± 0.019
0.372CysVal: 0.372 ± 0.019
0.063CysTrp: 0.063 ± 0.007
0.096CysTyr: 0.096 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
10.267AspAla: 10.267 ± 0.13
0.223AspCys: 0.223 ± 0.015
4.802AspAsp: 4.802 ± 0.087
4.386AspGlu: 4.386 ± 0.072
1.702AspPhe: 1.702 ± 0.042
6.674AspGly: 6.674 ± 0.1
1.162AspHis: 1.162 ± 0.033
2.533AspIle: 2.533 ± 0.05
0.94AspLys: 0.94 ± 0.033
6.39AspLeu: 6.39 ± 0.081
0.868AspMet: 0.868 ± 0.026
0.942AspAsn: 0.942 ± 0.044
4.349AspPro: 4.349 ± 0.065
1.506AspGln: 1.506 ± 0.036
4.816AspArg: 4.816 ± 0.079
2.595AspSer: 2.595 ± 0.058
3.008AspThr: 3.008 ± 0.051
6.068AspVal: 6.068 ± 0.077
0.942AspTrp: 0.942 ± 0.03
1.248AspTyr: 1.248 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
6.945GluAla: 6.945 ± 0.091
0.2GluCys: 0.2 ± 0.015
2.848GluAsp: 2.848 ± 0.051
3.04GluGlu: 3.04 ± 0.061
1.603GluPhe: 1.603 ± 0.041
4.009GluGly: 4.009 ± 0.059
1.279GluHis: 1.279 ± 0.039
2.537GluIle: 2.537 ± 0.048
1.309GluLys: 1.309 ± 0.041
6.101GluLeu: 6.101 ± 0.087
0.845GluMet: 0.845 ± 0.027
1.103GluAsn: 1.103 ± 0.034
3.02GluPro: 3.02 ± 0.063
1.9GluGln: 1.9 ± 0.044
5.038GluArg: 5.038 ± 0.089
2.798GluSer: 2.798 ± 0.06
3.027GluThr: 3.027 ± 0.058
4.596GluVal: 4.596 ± 0.083
0.863GluTrp: 0.863 ± 0.03
1.106GluTyr: 1.106 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
4.392PheAla: 4.392 ± 0.075
0.144PheCys: 0.144 ± 0.013
2.391PheAsp: 2.391 ± 0.055
1.623PheGlu: 1.623 ± 0.046
1.071PhePhe: 1.071 ± 0.039
3.324PheGly: 3.324 ± 0.062
0.54PheHis: 0.54 ± 0.024
1.127PheIle: 1.127 ± 0.037
0.383PheLys: 0.383 ± 0.019
2.944PheLeu: 2.944 ± 0.057
0.406PheMet: 0.406 ± 0.022
0.598PheAsn: 0.598 ± 0.025
1.461PhePro: 1.461 ± 0.039
0.766PheGln: 0.766 ± 0.027
1.861PheArg: 1.861 ± 0.047
1.766PheSer: 1.766 ± 0.039
2.157PheThr: 2.157 ± 0.053
2.796PheVal: 2.796 ± 0.061
0.487PheTrp: 0.487 ± 0.024
0.641PheTyr: 0.641 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
11.183GlyAla: 11.183 ± 0.103
0.518GlyCys: 0.518 ± 0.023
5.57GlyAsp: 5.57 ± 0.082
4.868GlyGlu: 4.868 ± 0.07
3.261GlyPhe: 3.261 ± 0.064
8.163GlyGly: 8.163 ± 0.131
1.752GlyHis: 1.752 ± 0.047
4.769GlyIle: 4.769 ± 0.068
1.833GlyLys: 1.833 ± 0.051
8.607GlyLeu: 8.607 ± 0.095
2.109GlyMet: 2.109 ± 0.044
1.602GlyAsn: 1.602 ± 0.043
4.01GlyPro: 4.01 ± 0.067
2.383GlyGln: 2.383 ± 0.052
6.877GlyArg: 6.877 ± 0.082
5.463GlySer: 5.463 ± 0.08
5.607GlyThr: 5.607 ± 0.092
8.258GlyVal: 8.258 ± 0.093
1.634GlyTrp: 1.634 ± 0.046
2.193GlyTyr: 2.193 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
2.328HisAla: 2.328 ± 0.052
0.103HisCys: 0.103 ± 0.01
1.392HisAsp: 1.392 ± 0.042
1.062HisGlu: 1.062 ± 0.031
0.559HisPhe: 0.559 ± 0.024
1.903HisGly: 1.903 ± 0.049
0.51HisHis: 0.51 ± 0.027
0.733HisIle: 0.733 ± 0.026
0.233HisLys: 0.233 ± 0.016
1.942HisLeu: 1.942 ± 0.045
0.303HisMet: 0.303 ± 0.018
0.304HisAsn: 0.304 ± 0.016
1.504HisPro: 1.504 ± 0.037
0.439HisGln: 0.439 ± 0.023
1.571HisArg: 1.571 ± 0.043
0.909HisSer: 0.909 ± 0.032
0.96HisThr: 0.96 ± 0.032
1.637HisVal: 1.637 ± 0.038
0.272HisTrp: 0.272 ± 0.017
0.389HisTyr: 0.389 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.994IleAla: 6.994 ± 0.091
0.23IleCys: 0.23 ± 0.015
3.431IleAsp: 3.431 ± 0.064
2.623IleGlu: 2.623 ± 0.047
1.193IlePhe: 1.193 ± 0.037
4.636IleGly: 4.636 ± 0.079
0.667IleHis: 0.667 ± 0.028
1.792IleIle: 1.792 ± 0.048
0.656IleLys: 0.656 ± 0.028
3.774IleLeu: 3.774 ± 0.075
0.583IleMet: 0.583 ± 0.026
0.776IleAsn: 0.776 ± 0.028
2.426IlePro: 2.426 ± 0.05
0.994IleGln: 0.994 ± 0.034
2.696IleArg: 2.696 ± 0.053
2.354IleSer: 2.354 ± 0.046
2.64IleThr: 2.64 ± 0.058
4.554IleVal: 4.554 ± 0.078
0.493IleTrp: 0.493 ± 0.021
0.73IleTyr: 0.73 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
2.185LysAla: 2.185 ± 0.055
0.055LysCys: 0.055 ± 0.007
1.091LysAsp: 1.091 ± 0.038
0.813LysGlu: 0.813 ± 0.034
0.406LysPhe: 0.406 ± 0.02
1.365LysGly: 1.365 ± 0.044
0.34LysHis: 0.34 ± 0.02
0.822LysIle: 0.822 ± 0.032
0.68LysLys: 0.68 ± 0.035
1.549LysLeu: 1.549 ± 0.041
0.326LysMet: 0.326 ± 0.019
0.456LysAsn: 0.456 ± 0.023
0.997LysPro: 0.997 ± 0.043
0.535LysGln: 0.535 ± 0.021
1.243LysArg: 1.243 ± 0.041
0.95LysSer: 0.95 ± 0.036
1.206LysThr: 1.206 ± 0.035
1.548LysVal: 1.548 ± 0.045
0.198LysTrp: 0.198 ± 0.013
0.375LysTyr: 0.375 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.277LeuAla: 14.277 ± 0.126
0.489LeuCys: 0.489 ± 0.025
6.594LeuAsp: 6.594 ± 0.097
5.004LeuGlu: 5.004 ± 0.079
2.861LeuPhe: 2.861 ± 0.066
8.922LeuGly: 8.922 ± 0.102
1.809LeuHis: 1.809 ± 0.046
4.444LeuIle: 4.444 ± 0.076
1.502LeuLys: 1.502 ± 0.038
10.035LeuLeu: 10.035 ± 0.141
1.515LeuMet: 1.515 ± 0.041
1.663LeuAsn: 1.663 ± 0.046
5.678LeuPro: 5.678 ± 0.082
2.608LeuGln: 2.608 ± 0.044
7.823LeuArg: 7.823 ± 0.1
5.982LeuSer: 5.982 ± 0.085
6.247LeuThr: 6.247 ± 0.079
9.061LeuVal: 9.061 ± 0.107
1.293LeuTrp: 1.293 ± 0.034
1.629LeuTyr: 1.629 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
2.111MetAla: 2.111 ± 0.048
0.082MetCys: 0.082 ± 0.01
0.849MetAsp: 0.849 ± 0.031
0.624MetGlu: 0.624 ± 0.026
0.483MetPhe: 0.483 ± 0.021
1.297MetGly: 1.297 ± 0.035
0.325MetHis: 0.325 ± 0.018
0.82MetIle: 0.82 ± 0.027
0.384MetLys: 0.384 ± 0.02
1.784MetLeu: 1.784 ± 0.046
0.29MetMet: 0.29 ± 0.02
0.441MetAsn: 0.441 ± 0.021
1.149MetPro: 1.149 ± 0.034
0.465MetGln: 0.465 ± 0.022
1.388MetArg: 1.388 ± 0.039
1.476MetSer: 1.476 ± 0.035
1.814MetThr: 1.814 ± 0.041
1.348MetVal: 1.348 ± 0.04
0.212MetTrp: 0.212 ± 0.014
0.287MetTyr: 0.287 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.299AsnAla: 2.299 ± 0.051
0.11AsnCys: 0.11 ± 0.011
1.074AsnAsp: 1.074 ± 0.038
0.883AsnGlu: 0.883 ± 0.031
0.575AsnPhe: 0.575 ± 0.029
1.707AsnGly: 1.707 ± 0.049
0.331AsnHis: 0.331 ± 0.017
0.77AsnIle: 0.77 ± 0.031
0.331AsnLys: 0.331 ± 0.021
1.698AsnLeu: 1.698 ± 0.038
0.289AsnMet: 0.289 ± 0.018
0.438AsnAsn: 0.438 ± 0.025
1.383AsnPro: 1.383 ± 0.037
0.486AsnGln: 0.486 ± 0.022
1.128AsnArg: 1.128 ± 0.032
0.944AsnSer: 0.944 ± 0.047
1.055AsnThr: 1.055 ± 0.039
1.538AsnVal: 1.538 ± 0.045
0.278AsnTrp: 0.278 ± 0.018
0.407AsnTyr: 0.407 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
8.013ProAla: 8.013 ± 0.104
0.163ProCys: 0.163 ± 0.012
4.169ProAsp: 4.169 ± 0.072
3.632ProGlu: 3.632 ± 0.069
1.783ProPhe: 1.783 ± 0.039
5.126ProGly: 5.126 ± 0.085
1.14ProHis: 1.14 ± 0.034
2.055ProIle: 2.055 ± 0.046
0.863ProLys: 0.863 ± 0.032
5.04ProLeu: 5.04 ± 0.068
0.867ProMet: 0.867 ± 0.029
0.906ProAsn: 0.906 ± 0.032
2.6ProPro: 2.6 ± 0.062
1.526ProGln: 1.526 ± 0.04
3.716ProArg: 3.716 ± 0.061
3.343ProSer: 3.343 ± 0.062
3.436ProThr: 3.436 ± 0.057
5.27ProVal: 5.27 ± 0.078
0.882ProTrp: 0.882 ± 0.031
1.074ProTyr: 1.074 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
3.262GlnAla: 3.262 ± 0.06
0.089GlnCys: 0.089 ± 0.008
1.394GlnAsp: 1.394 ± 0.036
1.251GlnGlu: 1.251 ± 0.035
0.805GlnPhe: 0.805 ± 0.029
2.122GlnGly: 2.122 ± 0.044
0.594GlnHis: 0.594 ± 0.023
1.299GlnIle: 1.299 ± 0.033
0.591GlnLys: 0.591 ± 0.024
2.968GlnLeu: 2.968 ± 0.052
0.473GlnMet: 0.473 ± 0.021
0.642GlnAsn: 0.642 ± 0.024
1.525GlnPro: 1.525 ± 0.042
1.084GlnGln: 1.084 ± 0.034
2.275GlnArg: 2.275 ± 0.049
1.514GlnSer: 1.514 ± 0.035
1.546GlnThr: 1.546 ± 0.037
2.299GlnVal: 2.299 ± 0.041
0.463GlnTrp: 0.463 ± 0.023
0.567GlnTyr: 0.567 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
9.441ArgAla: 9.441 ± 0.134
0.289ArgCys: 0.289 ± 0.019
4.609ArgAsp: 4.609 ± 0.076
4.285ArgGlu: 4.285 ± 0.074
2.455ArgPhe: 2.455 ± 0.051
5.771ArgGly: 5.771 ± 0.08
1.628ArgHis: 1.628 ± 0.042
3.639ArgIle: 3.639 ± 0.053
1.197ArgLys: 1.197 ± 0.035
7.522ArgLeu: 7.522 ± 0.106
1.784ArgMet: 1.784 ± 0.041
1.21ArgAsn: 1.21 ± 0.035
3.949ArgPro: 3.949 ± 0.063
1.96ArgGln: 1.96 ± 0.042
7.02ArgArg: 7.02 ± 0.106
4.315ArgSer: 4.315 ± 0.066
4.662ArgThr: 4.662 ± 0.073
6.413ArgVal: 6.413 ± 0.098
1.199ArgTrp: 1.199 ± 0.039
1.476ArgTyr: 1.476 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
7.584SerAla: 7.584 ± 0.087
0.244SerCys: 0.244 ± 0.017
3.548SerAsp: 3.548 ± 0.066
2.594SerGlu: 2.594 ± 0.052
1.981SerPhe: 1.981 ± 0.046
5.988SerGly: 5.988 ± 0.079
1.01SerHis: 1.01 ± 0.031
2.546SerIle: 2.546 ± 0.051
0.908SerLys: 0.908 ± 0.029
5.315SerLeu: 5.315 ± 0.069
1.171SerMet: 1.171 ± 0.029
0.961SerAsn: 0.961 ± 0.031
3.243SerPro: 3.243 ± 0.062
1.331SerGln: 1.331 ± 0.037
3.947SerArg: 3.947 ± 0.064
3.555SerSer: 3.555 ± 0.074
3.645SerThr: 3.645 ± 0.063
4.937SerVal: 4.937 ± 0.071
0.947SerTrp: 0.947 ± 0.028
1.124SerTyr: 1.124 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
8.244ThrAla: 8.244 ± 0.117
0.272ThrCys: 0.272 ± 0.017
3.754ThrAsp: 3.754 ± 0.062
2.821ThrGlu: 2.821 ± 0.052
1.842ThrPhe: 1.842 ± 0.044
5.851ThrGly: 5.851 ± 0.086
1.101ThrHis: 1.101 ± 0.034
2.742ThrIle: 2.742 ± 0.06
0.975ThrLys: 0.975 ± 0.027
6.05ThrLeu: 6.05 ± 0.078
0.976ThrMet: 0.976 ± 0.028
1.031ThrAsn: 1.031 ± 0.041
4.152ThrPro: 4.152 ± 0.071
1.434ThrGln: 1.434 ± 0.037
3.957ThrArg: 3.957 ± 0.065
3.5ThrSer: 3.5 ± 0.06
3.855ThrThr: 3.855 ± 0.072
6.102ThrVal: 6.102 ± 0.087
0.889ThrTrp: 0.889 ± 0.03
1.223ThrTyr: 1.223 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
12.186ValAla: 12.186 ± 0.125
0.498ValCys: 0.498 ± 0.022
6.148ValAsp: 6.148 ± 0.071
4.836ValGlu: 4.836 ± 0.069
2.936ValPhe: 2.936 ± 0.063
7.269ValGly: 7.269 ± 0.092
1.714ValHis: 1.714 ± 0.042
4.477ValIle: 4.477 ± 0.071
1.471ValLys: 1.471 ± 0.04
9.398ValLeu: 9.398 ± 0.12
1.497ValMet: 1.497 ± 0.05
1.743ValAsn: 1.743 ± 0.045
4.994ValPro: 4.994 ± 0.07
2.359ValGln: 2.359 ± 0.052
6.584ValArg: 6.584 ± 0.087
5.159ValSer: 5.159 ± 0.09
5.968ValThr: 5.968 ± 0.078
9.215ValVal: 9.215 ± 0.119
1.222ValTrp: 1.222 ± 0.042
1.565ValTyr: 1.565 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.614TrpAla: 1.614 ± 0.042
0.088TrpCys: 0.088 ± 0.009
0.812TrpAsp: 0.812 ± 0.029
0.625TrpGlu: 0.625 ± 0.025
0.602TrpPhe: 0.602 ± 0.026
1.201TrpGly: 1.201 ± 0.036
0.32TrpHis: 0.32 ± 0.018
0.714TrpIle: 0.714 ± 0.028
0.265TrpLys: 0.265 ± 0.018
1.66TrpLeu: 1.66 ± 0.045
0.375TrpMet: 0.375 ± 0.019
0.411TrpAsn: 0.411 ± 0.021
0.72TrpPro: 0.72 ± 0.027
0.501TrpGln: 0.501 ± 0.021
1.291TrpArg: 1.291 ± 0.039
0.954TrpSer: 0.954 ± 0.032
0.999TrpThr: 0.999 ± 0.032
1.18TrpVal: 1.18 ± 0.038
0.344TrpTrp: 0.344 ± 0.02
0.29TrpTyr: 0.29 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.449TyrAla: 2.449 ± 0.05
0.125TyrCys: 0.125 ± 0.013
1.385TyrAsp: 1.385 ± 0.041
1.084TyrGlu: 1.084 ± 0.029
0.676TyrPhe: 0.676 ± 0.031
1.923TyrGly: 1.923 ± 0.044
0.257TyrHis: 0.257 ± 0.014
0.724TyrIle: 0.724 ± 0.027
0.301TyrLys: 0.301 ± 0.02
2.012TyrLeu: 2.012 ± 0.038
0.257TyrMet: 0.257 ± 0.017
0.434TyrAsn: 0.434 ± 0.025
0.993TyrPro: 0.993 ± 0.032
0.547TyrGln: 0.547 ± 0.026
1.609TyrArg: 1.609 ± 0.043
1.063TyrSer: 1.063 ± 0.035
1.099TyrThr: 1.099 ± 0.034
1.614TyrVal: 1.614 ± 0.038
0.289TyrTrp: 0.289 ± 0.018
0.452TyrTyr: 0.452 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3276 proteins (1074964 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski