Amino acid dipepetide frequency for Phreatobacter cathodiphilus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.586AlaAla: 21.586 ± 0.227	1.141AlaCys: 1.141 ± 0.034	7.113AlaAsp: 7.113 ± 0.082	8.505AlaGlu: 8.505 ± 0.097	4.96AlaPhe: 4.96 ± 0.065	12.61AlaGly: 12.61 ± 0.116	2.313AlaHis: 2.313 ± 0.038	6.573AlaIle: 6.573 ± 0.077	3.846AlaLys: 3.846 ± 0.075	14.513AlaLeu: 14.513 ± 0.133	3.96AlaMet: 3.96 ± 0.059	2.616AlaAsn: 2.616 ± 0.046	6.753AlaPro: 6.753 ± 0.108	3.741AlaGln: 3.741 ± 0.06	10.322AlaArg: 10.322 ± 0.098	6.293AlaSer: 6.293 ± 0.07	6.79AlaThr: 6.79 ± 0.067	10.848AlaVal: 10.848 ± 0.12	1.674AlaTrp: 1.674 ± 0.037	2.596AlaTyr: 2.596 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.923CysAla: 0.923 ± 0.029	0.092CysCys: 0.092 ± 0.008	0.477CysAsp: 0.477 ± 0.018	0.396CysGlu: 0.396 ± 0.018	0.316CysPhe: 0.316 ± 0.015	0.892CysGly: 0.892 ± 0.03	0.21CysHis: 0.21 ± 0.012	0.36CysIle: 0.36 ± 0.015	0.135CysLys: 0.135 ± 0.01	0.732CysLeu: 0.732 ± 0.025	0.149CysMet: 0.149 ± 0.012	0.182CysAsn: 0.182 ± 0.012	0.441CysPro: 0.441 ± 0.02	0.192CysGln: 0.192 ± 0.012	0.671CysArg: 0.671 ± 0.026	0.369CysSer: 0.369 ± 0.017	0.411CysThr: 0.411 ± 0.022	0.558CysVal: 0.558 ± 0.022	0.092CysTrp: 0.092 ± 0.008	0.167CysTyr: 0.167 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	6.443AspAla: 6.443 ± 0.073	0.412AspCys: 0.412 ± 0.016	2.696AspAsp: 2.696 ± 0.053	3.135AspGlu: 3.135 ± 0.056	1.928AspPhe: 1.928 ± 0.037	5.1AspGly: 5.1 ± 0.071	1.183AspHis: 1.183 ± 0.034	2.802AspIle: 2.802 ± 0.053	1.376AspLys: 1.376 ± 0.036	6.004AspLeu: 6.004 ± 0.071	1.363AspMet: 1.363 ± 0.031	0.965AspAsn: 0.965 ± 0.027	3.702AspPro: 3.702 ± 0.055	1.546AspGln: 1.546 ± 0.032	4.761AspArg: 4.761 ± 0.065	1.676AspSer: 1.676 ± 0.035	2.4AspThr: 2.4 ± 0.048	4.054AspVal: 4.054 ± 0.053	0.858AspTrp: 0.858 ± 0.026	1.292AspTyr: 1.292 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	8.705GluAla: 8.705 ± 0.114	0.278GluCys: 0.278 ± 0.016	2.496GluAsp: 2.496 ± 0.052	3.052GluGlu: 3.052 ± 0.058	1.481GluPhe: 1.481 ± 0.032	4.533GluGly: 4.533 ± 0.067	0.986GluHis: 0.986 ± 0.025	3.031GluIle: 3.031 ± 0.048	1.934GluLys: 1.934 ± 0.047	4.354GluLeu: 4.354 ± 0.059	1.421GluMet: 1.421 ± 0.035	1.195GluAsn: 1.195 ± 0.03	2.774GluPro: 2.774 ± 0.052	1.656GluGln: 1.656 ± 0.042	5.067GluArg: 5.067 ± 0.067	1.983GluSer: 1.983 ± 0.037	3.452GluThr: 3.452 ± 0.053	4.005GluVal: 4.005 ± 0.062	0.586GluTrp: 0.586 ± 0.021	0.744GluTyr: 0.744 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.926PheAla: 4.926 ± 0.069	0.387PheCys: 0.387 ± 0.02	2.485PheAsp: 2.485 ± 0.039	1.896PheGlu: 1.896 ± 0.034	1.369PhePhe: 1.369 ± 0.036	3.881PheGly: 3.881 ± 0.06	0.734PheHis: 0.734 ± 0.026	1.548PheIle: 1.548 ± 0.038	0.899PheLys: 0.899 ± 0.024	3.25PheLeu: 3.25 ± 0.061	0.82PheMet: 0.82 ± 0.024	0.937PheAsn: 0.937 ± 0.026	1.624PhePro: 1.624 ± 0.033	0.962PheGln: 0.962 ± 0.026	2.517PheArg: 2.517 ± 0.05	1.96PheSer: 1.96 ± 0.041	1.984PheThr: 1.984 ± 0.041	3.106PheVal: 3.106 ± 0.047	0.53PheTrp: 0.53 ± 0.018	0.802PheTyr: 0.802 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	10.529GlyAla: 10.529 ± 0.098	0.793GlyCys: 0.793 ± 0.024	4.246GlyAsp: 4.246 ± 0.062	5.041GlyGlu: 5.041 ± 0.072	3.863GlyPhe: 3.863 ± 0.063	8.224GlyGly: 8.224 ± 0.092	1.958GlyHis: 1.958 ± 0.04	4.69GlyIle: 4.69 ± 0.063	2.795GlyLys: 2.795 ± 0.051	9.733GlyLeu: 9.733 ± 0.092	2.451GlyMet: 2.451 ± 0.046	1.822GlyAsn: 1.822 ± 0.039	4.272GlyPro: 4.272 ± 0.056	2.861GlyGln: 2.861 ± 0.059	7.528GlyArg: 7.528 ± 0.083	4.346GlySer: 4.346 ± 0.053	5.027GlyThr: 5.027 ± 0.066	6.291GlyVal: 6.291 ± 0.067	1.476GlyTrp: 1.476 ± 0.036	2.172GlyTyr: 2.172 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.48HisAla: 2.48 ± 0.049	0.196HisCys: 0.196 ± 0.013	1.125HisAsp: 1.125 ± 0.03	0.93HisGlu: 0.93 ± 0.033	0.752HisPhe: 0.752 ± 0.023	1.982HisGly: 1.982 ± 0.041	0.547HisHis: 0.547 ± 0.024	0.794HisIle: 0.794 ± 0.024	0.381HisLys: 0.381 ± 0.015	1.963HisLeu: 1.963 ± 0.042	0.428HisMet: 0.428 ± 0.017	0.386HisAsn: 0.386 ± 0.017	1.375HisPro: 1.375 ± 0.032	0.511HisGln: 0.511 ± 0.022	1.461HisArg: 1.461 ± 0.033	0.757HisSer: 0.757 ± 0.026	0.761HisThr: 0.761 ± 0.023	1.612HisVal: 1.612 ± 0.036	0.336HisTrp: 0.336 ± 0.016	0.462HisTyr: 0.462 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	7.574IleAla: 7.574 ± 0.089	0.447IleCys: 0.447 ± 0.018	3.203IleAsp: 3.203 ± 0.052	2.96IleGlu: 2.96 ± 0.051	1.491IlePhe: 1.491 ± 0.038	5.056IleGly: 5.056 ± 0.069	0.897IleHis: 0.897 ± 0.025	1.933IleIle: 1.933 ± 0.042	1.119IleLys: 1.119 ± 0.027	4.43IleLeu: 4.43 ± 0.066	0.922IleMet: 0.922 ± 0.026	1.149IleAsn: 1.149 ± 0.028	2.282IlePro: 2.282 ± 0.039	1.217IleGln: 1.217 ± 0.033	3.437IleArg: 3.437 ± 0.053	2.094IleSer: 2.094 ± 0.043	2.554IleThr: 2.554 ± 0.048	4.468IleVal: 4.468 ± 0.061	0.521IleTrp: 0.521 ± 0.021	0.929IleTyr: 0.929 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.219LysAla: 4.219 ± 0.072	0.118LysCys: 0.118 ± 0.01	1.442LysAsp: 1.442 ± 0.033	1.306LysGlu: 1.306 ± 0.031	0.735LysPhe: 0.735 ± 0.022	2.459LysGly: 2.459 ± 0.047	0.46LysHis: 0.46 ± 0.02	1.359LysIle: 1.359 ± 0.038	0.991LysLys: 0.991 ± 0.036	2.75LysLeu: 2.75 ± 0.05	0.585LysMet: 0.585 ± 0.021	0.613LysAsn: 0.613 ± 0.024	1.904LysPro: 1.904 ± 0.044	0.709LysGln: 0.709 ± 0.025	2.107LysArg: 2.107 ± 0.042	1.366LysSer: 1.366 ± 0.038	1.74LysThr: 1.74 ± 0.042	2.37LysVal: 2.37 ± 0.049	0.27LysTrp: 0.27 ± 0.015	0.455LysTyr: 0.455 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	16.032LeuAla: 16.032 ± 0.13	0.808LeuCys: 0.808 ± 0.027	5.813LeuAsp: 5.813 ± 0.08	4.365LeuGlu: 4.365 ± 0.064	3.614LeuPhe: 3.614 ± 0.067	8.66LeuGly: 8.66 ± 0.094	1.629LeuHis: 1.629 ± 0.034	4.424LeuIle: 4.424 ± 0.063	3.384LeuLys: 3.384 ± 0.055	8.523LeuLeu: 8.523 ± 0.115	2.472LeuMet: 2.472 ± 0.047	2.069LeuAsn: 2.069 ± 0.036	5.545LeuPro: 5.545 ± 0.074	2.298LeuGln: 2.298 ± 0.04	6.532LeuArg: 6.532 ± 0.071	5.684LeuSer: 5.684 ± 0.066	5.678LeuThr: 5.678 ± 0.062	8.629LeuVal: 8.629 ± 0.105	1.18LeuTrp: 1.18 ± 0.032	1.82LeuTyr: 1.82 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	3.561MetAla: 3.561 ± 0.052	0.144MetCys: 0.144 ± 0.011	1.147MetAsp: 1.147 ± 0.031	1.094MetGlu: 1.094 ± 0.031	0.698MetPhe: 0.698 ± 0.023	1.91MetGly: 1.91 ± 0.042	0.394MetHis: 0.394 ± 0.017	1.327MetIle: 1.327 ± 0.03	0.861MetLys: 0.861 ± 0.028	2.355MetLeu: 2.355 ± 0.046	0.7MetMet: 0.7 ± 0.026	0.672MetAsn: 0.672 ± 0.024	1.644MetPro: 1.644 ± 0.035	0.782MetGln: 0.782 ± 0.025	1.954MetArg: 1.954 ± 0.038	1.546MetSer: 1.546 ± 0.032	2.068MetThr: 2.068 ± 0.038	1.861MetVal: 1.861 ± 0.041	0.186MetTrp: 0.186 ± 0.012	0.291MetTyr: 0.291 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.87AsnAla: 2.87 ± 0.049	0.199AsnCys: 0.199 ± 0.012	1.082AsnAsp: 1.082 ± 0.029	0.982AsnGlu: 0.982 ± 0.029	0.762AsnPhe: 0.762 ± 0.025	1.965AsnGly: 1.965 ± 0.04	0.416AsnHis: 0.416 ± 0.022	1.065AsnIle: 1.065 ± 0.027	0.508AsnLys: 0.508 ± 0.02	2.247AsnLeu: 2.247 ± 0.044	0.492AsnMet: 0.492 ± 0.021	0.511AsnAsn: 0.511 ± 0.019	1.653AsnPro: 1.653 ± 0.034	0.62AsnGln: 0.62 ± 0.025	1.752AsnArg: 1.752 ± 0.033	0.876AsnSer: 0.876 ± 0.031	1.05AsnThr: 1.05 ± 0.031	1.746AsnVal: 1.746 ± 0.035	0.36AsnTrp: 0.36 ± 0.016	0.511AsnTyr: 0.511 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	8.214ProAla: 8.214 ± 0.109	0.312ProCys: 0.312 ± 0.015	3.533ProAsp: 3.533 ± 0.064	3.412ProGlu: 3.412 ± 0.058	2.153ProPhe: 2.153 ± 0.043	5.216ProGly: 5.216 ± 0.078	1.138ProHis: 1.138 ± 0.028	2.294ProIle: 2.294 ± 0.045	1.573ProLys: 1.573 ± 0.038	4.937ProLeu: 4.937 ± 0.065	1.302ProMet: 1.302 ± 0.029	1.234ProAsn: 1.234 ± 0.029	3.344ProPro: 3.344 ± 0.073	1.655ProGln: 1.655 ± 0.042	3.7ProArg: 3.7 ± 0.056	2.668ProSer: 2.668 ± 0.044	2.719ProThr: 2.719 ± 0.042	4.834ProVal: 4.834 ± 0.059	0.765ProTrp: 0.765 ± 0.024	1.145ProTyr: 1.145 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.15GlnAla: 4.15 ± 0.074	0.176GlnCys: 0.176 ± 0.011	1.243GlnAsp: 1.243 ± 0.032	1.243GlnGlu: 1.243 ± 0.033	0.924GlnPhe: 0.924 ± 0.028	2.323GlnGly: 2.323 ± 0.041	0.478GlnHis: 0.478 ± 0.019	1.684GlnIle: 1.684 ± 0.036	0.826GlnLys: 0.826 ± 0.027	2.382GlnLeu: 2.382 ± 0.046	0.79GlnMet: 0.79 ± 0.025	0.723GlnAsn: 0.723 ± 0.024	1.688GlnPro: 1.688 ± 0.04	1.122GlnGln: 1.122 ± 0.037	2.409GlnArg: 2.409 ± 0.047	1.438GlnSer: 1.438 ± 0.039	1.638GlnThr: 1.638 ± 0.037	2.362GlnVal: 2.362 ± 0.045	0.33GlnTrp: 0.33 ± 0.017	0.462GlnTyr: 0.462 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	9.168ArgAla: 9.168 ± 0.094	0.506ArgCys: 0.506 ± 0.02	4.289ArgAsp: 4.289 ± 0.068	4.412ArgGlu: 4.412 ± 0.061	3.229ArgPhe: 3.229 ± 0.052	5.593ArgGly: 5.593 ± 0.083	1.785ArgHis: 1.785 ± 0.04	4.222ArgIle: 4.222 ± 0.058	1.82ArgLys: 1.82 ± 0.04	8.488ArgLeu: 8.488 ± 0.093	2.051ArgMet: 2.051 ± 0.036	1.686ArgAsn: 1.686 ± 0.039	4.634ArgPro: 4.634 ± 0.073	2.652ArgGln: 2.652 ± 0.047	6.952ArgArg: 6.952 ± 0.087	3.843ArgSer: 3.843 ± 0.06	3.746ArgThr: 3.746 ± 0.055	5.308ArgVal: 5.308 ± 0.07	1.092ArgTrp: 1.092 ± 0.029	1.633ArgTyr: 1.633 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	5.698SerAla: 5.698 ± 0.083	0.357SerCys: 0.357 ± 0.019	2.405SerAsp: 2.405 ± 0.044	2.225SerGlu: 2.225 ± 0.041	2.071SerPhe: 2.071 ± 0.039	5.047SerGly: 5.047 ± 0.064	0.951SerHis: 0.951 ± 0.024	2.374SerIle: 2.374 ± 0.043	1.148SerLys: 1.148 ± 0.032	5.153SerLeu: 5.153 ± 0.067	1.194SerMet: 1.194 ± 0.03	1.077SerAsn: 1.077 ± 0.031	2.818SerPro: 2.818 ± 0.051	1.403SerGln: 1.403 ± 0.035	3.694SerArg: 3.694 ± 0.056	2.458SerSer: 2.458 ± 0.059	2.492SerThr: 2.492 ± 0.054	3.635SerVal: 3.635 ± 0.052	0.724SerTrp: 0.724 ± 0.02	1.111SerTyr: 1.111 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	7.013ThrAla: 7.013 ± 0.082	0.445ThrCys: 0.445 ± 0.019	2.722ThrAsp: 2.722 ± 0.046	2.478ThrGlu: 2.478 ± 0.057	2.046ThrPhe: 2.046 ± 0.044	5.423ThrGly: 5.423 ± 0.079	1.0ThrHis: 1.0 ± 0.028	2.909ThrIle: 2.909 ± 0.05	1.191ThrLys: 1.191 ± 0.036	5.893ThrLeu: 5.893 ± 0.065	1.207ThrMet: 1.207 ± 0.031	1.151ThrAsn: 1.151 ± 0.029	3.491ThrPro: 3.491 ± 0.054	1.32ThrGln: 1.32 ± 0.036	3.802ThrArg: 3.802 ± 0.059	2.693ThrSer: 2.693 ± 0.054	2.973ThrThr: 2.973 ± 0.061	4.852ThrVal: 4.852 ± 0.06	0.716ThrTrp: 0.716 ± 0.026	1.168ThrTyr: 1.168 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	10.998ValAla: 10.998 ± 0.103	0.59ValCys: 0.59 ± 0.02	4.322ValAsp: 4.322 ± 0.052	4.693ValGlu: 4.693 ± 0.06	2.937ValPhe: 2.937 ± 0.056	6.14ValGly: 6.14 ± 0.078	1.467ValHis: 1.467 ± 0.036	3.946ValIle: 3.946 ± 0.061	2.305ValLys: 2.305 ± 0.043	7.904ValLeu: 7.904 ± 0.095	2.127ValMet: 2.127 ± 0.037	1.835ValAsn: 1.835 ± 0.042	4.396ValPro: 4.396 ± 0.063	2.092ValGln: 2.092 ± 0.039	5.404ValArg: 5.404 ± 0.064	4.228ValSer: 4.228 ± 0.062	5.176ValThr: 5.176 ± 0.064	7.17ValVal: 7.17 ± 0.086	0.942ValTrp: 0.942 ± 0.027	1.423ValTyr: 1.423 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.246TrpAla: 1.246 ± 0.035	0.134TrpCys: 0.134 ± 0.009	0.589TrpAsp: 0.589 ± 0.023	0.524TrpGlu: 0.524 ± 0.018	0.529TrpPhe: 0.529 ± 0.018	0.994TrpGly: 0.994 ± 0.027	0.303TrpHis: 0.303 ± 0.016	0.67TrpIle: 0.67 ± 0.023	0.387TrpLys: 0.387 ± 0.019	1.519TrpLeu: 1.519 ± 0.033	0.354TrpMet: 0.354 ± 0.018	0.407TrpAsn: 0.407 ± 0.019	0.797TrpPro: 0.797 ± 0.024	0.484TrpGln: 0.484 ± 0.02	1.267TrpArg: 1.267 ± 0.03	0.793TrpSer: 0.793 ± 0.027	0.86TrpThr: 0.86 ± 0.028	0.845TrpVal: 0.845 ± 0.026	0.258TrpTrp: 0.258 ± 0.016	0.271TrpTyr: 0.271 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.418TyrAla: 2.418 ± 0.049	0.231TyrCys: 0.231 ± 0.012	1.27TyrAsp: 1.27 ± 0.035	1.083TyrGlu: 1.083 ± 0.033	0.761TyrPhe: 0.761 ± 0.025	2.109TyrGly: 2.109 ± 0.042	0.388TyrHis: 0.388 ± 0.017	0.679TyrIle: 0.679 ± 0.025	0.48TyrLys: 0.48 ± 0.019	2.031TyrLeu: 2.031 ± 0.046	0.405TyrMet: 0.405 ± 0.019	0.439TyrAsn: 0.439 ± 0.021	1.011TyrPro: 1.011 ± 0.027	0.568TyrGln: 0.568 ± 0.021	1.77TyrArg: 1.77 ± 0.035	0.962TyrSer: 0.962 ± 0.029	0.972TyrThr: 0.972 ± 0.026	1.542TyrVal: 1.542 ± 0.033	0.334TyrTrp: 0.334 ± 0.019	0.47TyrTyr: 0.47 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4219 proteins (1331878 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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