Rhodotorula graminis (strain WP1)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Microbotryomycetes; Sporidiobolales; Sporidiobolaceae; Rhodotorula; Rhodotorula graminis

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7228 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A194SAA9|A0A194SAA9_RHOGW Uncharacterized protein (Fragment) OS=Rhodotorula graminis (strain WP1) OX=578459 GN=RHOBADRAFT_65998 PE=4 SV=1
MM1 pKa = 7.72LGRR4 pKa = 11.84ATAAALVVLAGVAQAIVVSSPTATSVWSQSADD36 pKa = 2.73GDD38 pKa = 4.36ARR40 pKa = 11.84IEE42 pKa = 4.08WSLLPATSPPPATQYY57 pKa = 11.15FDD59 pKa = 2.92IYY61 pKa = 10.61LRR63 pKa = 11.84NGVGAMYY70 pKa = 9.65TPSLNLLLATKK81 pKa = 10.12VDD83 pKa = 4.3SLASTYY89 pKa = 11.08LDD91 pKa = 3.22VAATDD96 pKa = 3.78KK97 pKa = 10.69FVPGPGYY104 pKa = 10.13QLFFSDD110 pKa = 4.12PANPATVYY118 pKa = 10.61CDD120 pKa = 3.09SDD122 pKa = 3.72VFAIGSYY129 pKa = 9.7EE130 pKa = 3.95INGPSPTSSSSSDD143 pKa = 3.14VDD145 pKa = 3.63SSTQAEE151 pKa = 4.19ASASSTEE158 pKa = 3.92TRR160 pKa = 11.84AAAAPTSTEE169 pKa = 3.89EE170 pKa = 4.23TTVEE174 pKa = 4.01PSSTEE179 pKa = 3.86TLSYY183 pKa = 10.76EE184 pKa = 4.18PASTDD189 pKa = 3.22STSTSSGMITSVTSSSSPSPAPVSSSSSSAPAGGITAAPAPGGPNEE235 pKa = 4.03QGFNLVPSGASGVRR249 pKa = 11.84AGLSAAAGLSLLAGAGAALLLL270 pKa = 4.39

Molecular weight: 26.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A194S7E0|A0A194S7E0_RHOGW Uncharacterized protein OS=Rhodotorula graminis (strain WP1) OX=578459 GN=RHOBADRAFT_43836 PE=4 SV=1
RR1 pKa = 7.23RR2 pKa = 11.84TRR4 pKa = 11.84TCTLRR9 pKa = 11.84IAQSTRR15 pKa = 11.84PTSLLPTHH23 pKa = 7.24PSPTSPSSRR32 pKa = 11.84PSRR35 pKa = 11.84TRR37 pKa = 11.84TPSSRR42 pKa = 11.84RR43 pKa = 11.84PTRR46 pKa = 11.84PLPLPLLPRR55 pKa = 11.84TRR57 pKa = 11.84PPGRR61 pKa = 11.84SRR63 pKa = 11.84SATRR67 pKa = 11.84PSRR70 pKa = 11.84RR71 pKa = 11.84TRR73 pKa = 11.84ARR75 pKa = 11.84PRR77 pKa = 11.84SRR79 pKa = 11.84RR80 pKa = 11.84PTRR83 pKa = 11.84PATAAAPSRR92 pKa = 11.84PRR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84PARR99 pKa = 11.84PRR101 pKa = 11.84PTSRR105 pKa = 11.84RR106 pKa = 11.84RR107 pKa = 11.84SPSPTRR113 pKa = 11.84LPTATPTASLRR124 pKa = 11.84AASRR128 pKa = 11.84SSSPRR133 pKa = 11.84TAASRR138 pKa = 11.84LLSRR142 pKa = 11.84RR143 pKa = 11.84SATSTAPTSRR153 pKa = 11.84AARR156 pKa = 11.84RR157 pKa = 11.84RR158 pKa = 11.84PSAASTSRR166 pKa = 11.84AAVRR170 pKa = 11.84GRR172 pKa = 11.84RR173 pKa = 11.84TAPAGPSGRR182 pKa = 11.84RR183 pKa = 11.84GRR185 pKa = 11.84PTASRR190 pKa = 11.84SRR192 pKa = 11.84RR193 pKa = 11.84RR194 pKa = 11.84LSARR198 pKa = 11.84RR199 pKa = 11.84SPRR202 pKa = 11.84TSRR205 pKa = 11.84AASTRR210 pKa = 11.84ATSATSALLARR221 pKa = 11.84APSRR225 pKa = 11.84LTFSRR230 pKa = 11.84TPRR233 pKa = 11.84RR234 pKa = 11.84SRR236 pKa = 11.84SSAAPATEE244 pKa = 4.91GSPSCPTSAVTAAFVATPSRR264 pKa = 11.84PSKK267 pKa = 9.64SRR269 pKa = 11.84RR270 pKa = 11.84RR271 pKa = 11.84ARR273 pKa = 11.84GRR275 pKa = 11.84LSVRR279 pKa = 11.84HH280 pKa = 5.04RR281 pKa = 11.84AGRR284 pKa = 11.84RR285 pKa = 11.84AAAGLARR292 pKa = 11.84PRR294 pKa = 11.84SCPTRR299 pKa = 11.84VRR301 pKa = 11.84HH302 pKa = 6.55RR303 pKa = 11.84YY304 pKa = 8.82GLSHH308 pKa = 7.64LRR310 pKa = 11.84RR311 pKa = 11.84QGCRR315 pKa = 11.84RR316 pKa = 11.84RR317 pKa = 11.84GTSPHH322 pKa = 6.08FFFPRR327 pKa = 11.84TLPLVVRR334 pKa = 11.84FF335 pKa = 4.2

Molecular weight: 36.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7225

3

7228

3636073

49

5410

503.1

54.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.082 ± 0.035

1.0 ± 0.01

5.906 ± 0.024

5.689 ± 0.025

3.208 ± 0.018

7.62 ± 0.03

2.423 ± 0.013

3.07 ± 0.021

3.774 ± 0.029

9.423 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.531 ± 0.011

2.175 ± 0.016

7.219 ± 0.041

3.424 ± 0.02

7.254 ± 0.038

8.736 ± 0.041

5.396 ± 0.018

6.663 ± 0.025

1.262 ± 0.011

2.146 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski