Rhizobium sp. ACO-34A

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5675 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A292E582|A0A292E582_9RHIZ Methyltransferase OS=Rhizobium sp. ACO-34A OX=1571470 GN=ACO34A_08335 PE=3 SV=1
MM1 pKa = 7.56SVLKK5 pKa = 10.62YY6 pKa = 10.33AALSLCLVATPALSDD21 pKa = 3.99DD22 pKa = 4.09LVALSTTATAITGDD36 pKa = 3.02ISFDD40 pKa = 3.31DD41 pKa = 4.08FEE43 pKa = 5.17IVFEE47 pKa = 4.24NGKK50 pKa = 9.9KK51 pKa = 10.25LVFEE55 pKa = 5.39DD56 pKa = 5.58LIADD60 pKa = 3.75NFIVGGKK67 pKa = 7.98EE68 pKa = 3.86VPASVYY74 pKa = 9.74SVKK77 pKa = 10.03GTTNPKK83 pKa = 9.8LLNGNRR89 pKa = 11.84LCGASPVTYY98 pKa = 10.04LASWLDD104 pKa = 3.7DD105 pKa = 4.24DD106 pKa = 4.26VTMIAVFEE114 pKa = 4.31TQDD117 pKa = 3.44APEE120 pKa = 4.64SDD122 pKa = 4.26EE123 pKa = 5.59DD124 pKa = 3.59MCALYY129 pKa = 9.68TYY131 pKa = 9.79VYY133 pKa = 10.06PP134 pKa = 5.13

Molecular weight: 14.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A292E2B1|A0A292E2B1_9RHIZ TIGR02300 family protein OS=Rhizobium sp. ACO-34A OX=1571470 GN=ACO34A_00975 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.64GFRR19 pKa = 11.84ARR21 pKa = 11.84IATKK25 pKa = 10.29GGRR28 pKa = 11.84KK29 pKa = 9.26VIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5675

0

5675

1804250

29

2830

317.9

34.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.774 ± 0.036

0.819 ± 0.01

5.674 ± 0.028

5.921 ± 0.029

3.937 ± 0.021

8.454 ± 0.037

1.998 ± 0.016

5.657 ± 0.023

3.577 ± 0.027

10.007 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.594 ± 0.015

2.783 ± 0.018

4.906 ± 0.022

2.964 ± 0.02

6.693 ± 0.037

5.892 ± 0.027

5.382 ± 0.027

7.384 ± 0.024

1.271 ± 0.014

2.313 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski