Amino acid dipepetide frequency for Rhizobium sp. ACO-34A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.192AlaAla: 15.192 ± 0.146
0.959AlaCys: 0.959 ± 0.025
6.633AlaAsp: 6.633 ± 0.065
7.573AlaGlu: 7.573 ± 0.083
4.594AlaPhe: 4.594 ± 0.055
10.628AlaGly: 10.628 ± 0.086
2.001AlaHis: 2.001 ± 0.032
6.905AlaIle: 6.905 ± 0.058
3.938AlaLys: 3.938 ± 0.053
12.461AlaLeu: 12.461 ± 0.122
3.468AlaMet: 3.468 ± 0.045
2.966AlaAsn: 2.966 ± 0.046
4.631AlaPro: 4.631 ± 0.069
3.141AlaGln: 3.141 ± 0.048
7.766AlaArg: 7.766 ± 0.072
6.687AlaSer: 6.687 ± 0.067
5.948AlaThr: 5.948 ± 0.067
8.427AlaVal: 8.427 ± 0.081
1.27AlaTrp: 1.27 ± 0.029
2.555AlaTyr: 2.555 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
0.873CysAla: 0.873 ± 0.027
0.119CysCys: 0.119 ± 0.01
0.495CysAsp: 0.495 ± 0.017
0.438CysGlu: 0.438 ± 0.015
0.319CysPhe: 0.319 ± 0.015
0.884CysGly: 0.884 ± 0.025
0.243CysHis: 0.243 ± 0.011
0.401CysIle: 0.401 ± 0.015
0.205CysLys: 0.205 ± 0.012
0.795CysLeu: 0.795 ± 0.021
0.174CysMet: 0.174 ± 0.008
0.222CysAsn: 0.222 ± 0.011
0.402CysPro: 0.402 ± 0.017
0.215CysGln: 0.215 ± 0.011
0.62CysArg: 0.62 ± 0.02
0.473CysSer: 0.473 ± 0.018
0.394CysThr: 0.394 ± 0.015
0.596CysVal: 0.596 ± 0.02
0.117CysTrp: 0.117 ± 0.008
0.204CysTyr: 0.204 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.328AspAla: 6.328 ± 0.062
0.491AspCys: 0.491 ± 0.019
3.103AspAsp: 3.103 ± 0.052
3.604AspGlu: 3.604 ± 0.05
2.435AspPhe: 2.435 ± 0.042
5.021AspGly: 5.021 ± 0.06
1.24AspHis: 1.24 ± 0.028
3.494AspIle: 3.494 ± 0.049
1.824AspLys: 1.824 ± 0.034
5.796AspLeu: 5.796 ± 0.064
1.465AspMet: 1.465 ± 0.028
1.454AspAsn: 1.454 ± 0.027
3.336AspPro: 3.336 ± 0.043
1.569AspGln: 1.569 ± 0.037
4.147AspArg: 4.147 ± 0.05
2.168AspSer: 2.168 ± 0.035
2.632AspThr: 2.632 ± 0.04
4.12AspVal: 4.12 ± 0.054
0.955AspTrp: 0.955 ± 0.023
1.556AspTyr: 1.556 ± 0.032
0.0AspXaa: 0.0 ± 0.0
Glu
7.491GluAla: 7.491 ± 0.066
0.379GluCys: 0.379 ± 0.015
2.945GluAsp: 2.945 ± 0.041
3.633GluGlu: 3.633 ± 0.049
1.896GluPhe: 1.896 ± 0.03
4.466GluGly: 4.466 ± 0.052
1.192GluHis: 1.192 ± 0.026
3.927GluIle: 3.927 ± 0.056
2.965GluLys: 2.965 ± 0.04
5.397GluLeu: 5.397 ± 0.056
1.575GluMet: 1.575 ± 0.029
1.98GluAsn: 1.98 ± 0.033
2.661GluPro: 2.661 ± 0.043
2.119GluGln: 2.119 ± 0.043
4.687GluArg: 4.687 ± 0.059
2.409GluSer: 2.409 ± 0.034
3.848GluThr: 3.848 ± 0.051
3.827GluVal: 3.827 ± 0.043
0.758GluTrp: 0.758 ± 0.019
1.056GluTyr: 1.056 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
4.516PheAla: 4.516 ± 0.051
0.438PheCys: 0.438 ± 0.017
2.782PheAsp: 2.782 ± 0.045
2.283PheGlu: 2.283 ± 0.034
1.617PhePhe: 1.617 ± 0.037
3.831PheGly: 3.831 ± 0.052
0.809PheHis: 0.809 ± 0.021
1.987PheIle: 1.987 ± 0.034
1.069PheLys: 1.069 ± 0.027
3.643PheLeu: 3.643 ± 0.05
0.932PheMet: 0.932 ± 0.024
1.123PheAsn: 1.123 ± 0.027
1.664PhePro: 1.664 ± 0.032
1.091PheGln: 1.091 ± 0.024
2.366PheArg: 2.366 ± 0.037
2.771PheSer: 2.771 ± 0.041
2.0PheThr: 2.0 ± 0.03
2.962PheVal: 2.962 ± 0.045
0.529PheTrp: 0.529 ± 0.017
0.959PheTyr: 0.959 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
8.506GlyAla: 8.506 ± 0.076
0.823GlyCys: 0.823 ± 0.025
4.332GlyAsp: 4.332 ± 0.056
4.94GlyGlu: 4.94 ± 0.065
3.781GlyPhe: 3.781 ± 0.045
7.053GlyGly: 7.053 ± 0.084
1.865GlyHis: 1.865 ± 0.036
5.146GlyIle: 5.146 ± 0.049
3.729GlyLys: 3.729 ± 0.053
8.835GlyLeu: 8.835 ± 0.093
2.374GlyMet: 2.374 ± 0.038
2.435GlyAsn: 2.435 ± 0.053
3.183GlyPro: 3.183 ± 0.047
2.655GlyGln: 2.655 ± 0.037
5.799GlyArg: 5.799 ± 0.057
4.973GlySer: 4.973 ± 0.061
4.512GlyThr: 4.512 ± 0.067
5.883GlyVal: 5.883 ± 0.049
1.283GlyTrp: 1.283 ± 0.03
2.438GlyTyr: 2.438 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
2.154HisAla: 2.154 ± 0.037
0.206HisCys: 0.206 ± 0.012
1.269HisAsp: 1.269 ± 0.027
1.087HisGlu: 1.087 ± 0.026
0.931HisPhe: 0.931 ± 0.022
1.867HisGly: 1.867 ± 0.03
0.569HisHis: 0.569 ± 0.024
1.033HisIle: 1.033 ± 0.027
0.522HisLys: 0.522 ± 0.016
2.007HisLeu: 2.007 ± 0.034
0.545HisMet: 0.545 ± 0.018
0.471HisAsn: 0.471 ± 0.016
1.285HisPro: 1.285 ± 0.029
0.581HisGln: 0.581 ± 0.017
1.317HisArg: 1.317 ± 0.025
1.014HisSer: 1.014 ± 0.027
0.793HisThr: 0.793 ± 0.023
1.481HisVal: 1.481 ± 0.027
0.313HisTrp: 0.313 ± 0.014
0.53HisTyr: 0.53 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
7.752IleAla: 7.752 ± 0.095
0.57IleCys: 0.57 ± 0.018
3.924IleAsp: 3.924 ± 0.051
3.822IleGlu: 3.822 ± 0.052
1.999IlePhe: 1.999 ± 0.036
5.427IleGly: 5.427 ± 0.063
0.98IleHis: 0.98 ± 0.023
2.658IleIle: 2.658 ± 0.039
1.557IleLys: 1.557 ± 0.034
4.901IleLeu: 4.901 ± 0.052
1.165IleMet: 1.165 ± 0.029
1.57IleAsn: 1.57 ± 0.031
2.534IlePro: 2.534 ± 0.033
1.209IleGln: 1.209 ± 0.029
3.657IleArg: 3.657 ± 0.043
3.523IleSer: 3.523 ± 0.054
2.882IleThr: 2.882 ± 0.037
4.55IleVal: 4.55 ± 0.047
0.646IleTrp: 0.646 ± 0.018
1.243IleTyr: 1.243 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.663LysAla: 4.663 ± 0.055
0.156LysCys: 0.156 ± 0.009
1.876LysAsp: 1.876 ± 0.034
1.897LysGlu: 1.897 ± 0.035
0.995LysPhe: 0.995 ± 0.024
2.868LysGly: 2.868 ± 0.041
0.583LysHis: 0.583 ± 0.018
2.02LysIle: 2.02 ± 0.033
1.462LysLys: 1.462 ± 0.031
3.567LysLeu: 3.567 ± 0.052
0.866LysMet: 0.866 ± 0.02
1.0LysAsn: 1.0 ± 0.021
2.209LysPro: 2.209 ± 0.039
1.053LysGln: 1.053 ± 0.026
2.422LysArg: 2.422 ± 0.041
2.153LysSer: 2.153 ± 0.038
2.2LysThr: 2.2 ± 0.036
2.724LysVal: 2.724 ± 0.043
0.405LysTrp: 0.405 ± 0.018
0.651LysTyr: 0.651 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
12.813LeuAla: 12.813 ± 0.105
0.868LeuCys: 0.868 ± 0.021
5.914LeuAsp: 5.914 ± 0.061
5.335LeuGlu: 5.335 ± 0.055
3.808LeuPhe: 3.808 ± 0.051
7.995LeuGly: 7.995 ± 0.082
1.751LeuHis: 1.751 ± 0.033
5.148LeuIle: 5.148 ± 0.057
4.099LeuLys: 4.099 ± 0.05
9.534LeuLeu: 9.534 ± 0.102
2.436LeuMet: 2.436 ± 0.038
2.653LeuAsn: 2.653 ± 0.038
5.43LeuPro: 5.43 ± 0.057
2.759LeuGln: 2.759 ± 0.043
6.09LeuArg: 6.09 ± 0.06
7.235LeuSer: 7.235 ± 0.091
5.626LeuThr: 5.626 ± 0.065
7.412LeuVal: 7.412 ± 0.069
1.071LeuTrp: 1.071 ± 0.029
2.098LeuTyr: 2.098 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
3.12MetAla: 3.12 ± 0.042
0.155MetCys: 0.155 ± 0.009
1.184MetAsp: 1.184 ± 0.024
1.35MetGlu: 1.35 ± 0.025
0.749MetPhe: 0.749 ± 0.018
1.706MetGly: 1.706 ± 0.031
0.438MetHis: 0.438 ± 0.016
1.511MetIle: 1.511 ± 0.032
1.106MetLys: 1.106 ± 0.028
2.573MetLeu: 2.573 ± 0.04
0.727MetMet: 0.727 ± 0.022
0.884MetAsn: 0.884 ± 0.022
1.565MetPro: 1.565 ± 0.03
0.885MetGln: 0.885 ± 0.023
1.795MetArg: 1.795 ± 0.033
1.758MetSer: 1.758 ± 0.032
2.048MetThr: 2.048 ± 0.036
1.861MetVal: 1.861 ± 0.033
0.207MetTrp: 0.207 ± 0.01
0.32MetTyr: 0.32 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.234AsnAla: 3.234 ± 0.042
0.226AsnCys: 0.226 ± 0.011
1.594AsnAsp: 1.594 ± 0.041
1.397AsnGlu: 1.397 ± 0.027
1.079AsnPhe: 1.079 ± 0.024
2.637AsnGly: 2.637 ± 0.043
0.544AsnHis: 0.544 ± 0.016
1.556AsnIle: 1.556 ± 0.031
0.74AsnLys: 0.74 ± 0.018
2.714AsnLeu: 2.714 ± 0.043
0.703AsnMet: 0.703 ± 0.017
0.761AsnAsn: 0.761 ± 0.024
1.867AsnPro: 1.867 ± 0.035
0.773AsnGln: 0.773 ± 0.018
2.016AsnArg: 2.016 ± 0.038
1.451AsnSer: 1.451 ± 0.03
1.347AsnThr: 1.347 ± 0.03
2.021AsnVal: 2.021 ± 0.036
0.477AsnTrp: 0.477 ± 0.018
0.697AsnTyr: 0.697 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
5.767ProAla: 5.767 ± 0.072
0.274ProCys: 0.274 ± 0.011
3.436ProAsp: 3.436 ± 0.042
3.773ProGlu: 3.773 ± 0.052
2.006ProPhe: 2.006 ± 0.034
4.036ProGly: 4.036 ± 0.053
1.051ProHis: 1.051 ± 0.025
2.263ProIle: 2.263 ± 0.036
1.796ProLys: 1.796 ± 0.032
4.703ProLeu: 4.703 ± 0.058
1.171ProMet: 1.171 ± 0.03
1.234ProAsn: 1.234 ± 0.028
2.106ProPro: 2.106 ± 0.044
1.561ProGln: 1.561 ± 0.033
2.533ProArg: 2.533 ± 0.039
2.795ProSer: 2.795 ± 0.04
2.347ProThr: 2.347 ± 0.035
4.358ProVal: 4.358 ± 0.052
0.612ProTrp: 0.612 ± 0.017
1.24ProTyr: 1.24 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.597GlnAla: 3.597 ± 0.049
0.184GlnCys: 0.184 ± 0.011
1.344GlnAsp: 1.344 ± 0.031
1.588GlnGlu: 1.588 ± 0.03
1.068GlnPhe: 1.068 ± 0.025
2.108GlnGly: 2.108 ± 0.031
0.6GlnHis: 0.6 ± 0.018
1.892GlnIle: 1.892 ± 0.051
1.205GlnLys: 1.205 ± 0.029
2.712GlnLeu: 2.712 ± 0.044
0.868GlnMet: 0.868 ± 0.022
0.893GlnAsn: 0.893 ± 0.022
1.675GlnPro: 1.675 ± 0.036
1.241GlnGln: 1.241 ± 0.032
2.112GlnArg: 2.112 ± 0.04
1.857GlnSer: 1.857 ± 0.029
1.635GlnThr: 1.635 ± 0.027
2.071GlnVal: 2.071 ± 0.04
0.386GlnTrp: 0.386 ± 0.016
0.6GlnTyr: 0.6 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
6.72ArgAla: 6.72 ± 0.056
0.441ArgCys: 0.441 ± 0.017
3.749ArgAsp: 3.749 ± 0.042
4.062ArgGlu: 4.062 ± 0.052
2.951ArgPhe: 2.951 ± 0.042
4.38ArgGly: 4.38 ± 0.05
1.706ArgHis: 1.706 ± 0.031
4.166ArgIle: 4.166 ± 0.056
2.578ArgLys: 2.578 ± 0.035
7.338ArgLeu: 7.338 ± 0.078
1.924ArgMet: 1.924 ± 0.033
2.09ArgAsn: 2.09 ± 0.038
3.228ArgPro: 3.228 ± 0.049
2.553ArgGln: 2.553 ± 0.033
5.168ArgArg: 5.168 ± 0.069
3.801ArgSer: 3.801 ± 0.051
3.161ArgThr: 3.161 ± 0.048
4.302ArgVal: 4.302 ± 0.051
0.864ArgTrp: 0.864 ± 0.02
1.744ArgTyr: 1.744 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.533SerAla: 6.533 ± 0.071
0.424SerCys: 0.424 ± 0.014
3.201SerAsp: 3.201 ± 0.048
3.19SerGlu: 3.19 ± 0.045
2.557SerPhe: 2.557 ± 0.037
6.059SerGly: 6.059 ± 0.067
1.202SerHis: 1.202 ± 0.026
3.261SerIle: 3.261 ± 0.04
1.796SerLys: 1.796 ± 0.034
6.09SerLeu: 6.09 ± 0.067
1.489SerMet: 1.489 ± 0.03
1.537SerAsn: 1.537 ± 0.028
2.832SerPro: 2.832 ± 0.041
1.657SerGln: 1.657 ± 0.037
3.894SerArg: 3.894 ± 0.059
3.558SerSer: 3.558 ± 0.063
3.075SerThr: 3.075 ± 0.083
4.347SerVal: 4.347 ± 0.059
0.738SerTrp: 0.738 ± 0.022
1.482SerTyr: 1.482 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
6.175ThrAla: 6.175 ± 0.077
0.436ThrCys: 0.436 ± 0.018
2.913ThrAsp: 2.913 ± 0.048
2.758ThrGlu: 2.758 ± 0.037
2.094ThrPhe: 2.094 ± 0.035
5.174ThrGly: 5.174 ± 0.063
0.995ThrHis: 0.995 ± 0.022
3.204ThrIle: 3.204 ± 0.046
1.508ThrLys: 1.508 ± 0.028
5.717ThrLeu: 5.717 ± 0.067
1.286ThrMet: 1.286 ± 0.027
1.401ThrAsn: 1.401 ± 0.032
3.057ThrPro: 3.057 ± 0.043
1.391ThrGln: 1.391 ± 0.03
3.224ThrArg: 3.224 ± 0.049
3.109ThrSer: 3.109 ± 0.05
2.919ThrThr: 2.919 ± 0.05
4.583ThrVal: 4.583 ± 0.056
0.621ThrTrp: 0.621 ± 0.02
1.254ThrTyr: 1.254 ± 0.03
0.0ThrXaa: 0.0 ± 0.0
Val
8.765ValAla: 8.765 ± 0.071
0.666ValCys: 0.666 ± 0.02
3.996ValAsp: 3.996 ± 0.049
4.595ValGlu: 4.595 ± 0.055
2.971ValPhe: 2.971 ± 0.045
5.437ValGly: 5.437 ± 0.063
1.393ValHis: 1.393 ± 0.03
4.286ValIle: 4.286 ± 0.053
2.461ValLys: 2.461 ± 0.048
7.362ValLeu: 7.362 ± 0.08
1.92ValMet: 1.92 ± 0.035
2.031ValAsn: 2.031 ± 0.037
3.606ValPro: 3.606 ± 0.044
1.897ValGln: 1.897 ± 0.034
4.509ValArg: 4.509 ± 0.051
4.964ValSer: 4.964 ± 0.054
4.605ValThr: 4.605 ± 0.057
5.914ValVal: 5.914 ± 0.064
0.873ValTrp: 0.873 ± 0.023
1.585ValTyr: 1.585 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.117TrpAla: 1.117 ± 0.028
0.117TrpCys: 0.117 ± 0.007
0.574TrpAsp: 0.574 ± 0.019
0.586TrpGlu: 0.586 ± 0.016
0.54TrpPhe: 0.54 ± 0.016
0.801TrpGly: 0.801 ± 0.021
0.311TrpHis: 0.311 ± 0.013
0.691TrpIle: 0.691 ± 0.022
0.536TrpLys: 0.536 ± 0.016
1.577TrpLeu: 1.577 ± 0.035
0.354TrpMet: 0.354 ± 0.014
0.479TrpAsn: 0.479 ± 0.015
0.657TrpPro: 0.657 ± 0.02
0.554TrpGln: 0.554 ± 0.018
1.02TrpArg: 1.02 ± 0.024
0.848TrpSer: 0.848 ± 0.025
0.716TrpThr: 0.716 ± 0.021
0.734TrpVal: 0.734 ± 0.018
0.216TrpTrp: 0.216 ± 0.011
0.28TrpTyr: 0.28 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.428TyrAla: 2.428 ± 0.04
0.257TyrCys: 0.257 ± 0.013
1.473TyrAsp: 1.473 ± 0.027
1.3TyrGlu: 1.3 ± 0.029
0.984TyrPhe: 0.984 ± 0.027
2.166TyrGly: 2.166 ± 0.038
0.499TyrHis: 0.499 ± 0.017
1.02TyrIle: 1.02 ± 0.025
0.671TyrLys: 0.671 ± 0.021
2.353TyrLeu: 2.353 ± 0.036
0.5TyrMet: 0.5 ± 0.016
0.648TyrAsn: 0.648 ± 0.023
1.135TyrPro: 1.135 ± 0.026
0.735TyrGln: 0.735 ± 0.021
1.787TyrArg: 1.787 ± 0.034
1.375TyrSer: 1.375 ± 0.029
1.129TyrThr: 1.129 ± 0.031
1.664TyrVal: 1.664 ± 0.034
0.367TyrTrp: 0.367 ± 0.015
0.644TyrTyr: 0.644 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5675 proteins (1804250 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski