Amino acid dipepetide frequency for Gordonia phage Boneham

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.051AlaAla: 13.051 ± 3.109
0.782AlaCys: 0.782 ± 0.216
5.187AlaAsp: 5.187 ± 0.488
6.093AlaGlu: 6.093 ± 0.561
2.758AlaPhe: 2.758 ± 0.433
7.493AlaGly: 7.493 ± 0.676
1.194AlaHis: 1.194 ± 0.207
5.064AlaIle: 5.064 ± 0.632
4.405AlaLys: 4.405 ± 0.56
7.287AlaLeu: 7.287 ± 0.912
2.758AlaMet: 2.758 ± 0.469
3.417AlaAsn: 3.417 ± 0.502
4.693AlaPro: 4.693 ± 0.603
4.282AlaGln: 4.282 ± 0.857
4.899AlaArg: 4.899 ± 0.601
6.34AlaSer: 6.34 ± 0.709
5.723AlaThr: 5.723 ± 0.509
5.97AlaVal: 5.97 ± 0.618
1.894AlaTrp: 1.894 ± 0.465
2.882AlaTyr: 2.882 ± 0.309
0.0AlaXaa: 0.0 ± 0.0
Cys
0.7CysAla: 0.7 ± 0.186
0.165CysCys: 0.165 ± 0.087
0.823CysAsp: 0.823 ± 0.252
0.782CysGlu: 0.782 ± 0.192
0.206CysPhe: 0.206 ± 0.099
0.906CysGly: 0.906 ± 0.208
0.206CysHis: 0.206 ± 0.112
0.576CysIle: 0.576 ± 0.146
0.618CysLys: 0.618 ± 0.21
0.659CysLeu: 0.659 ± 0.19
0.082CysMet: 0.082 ± 0.062
0.535CysAsn: 0.535 ± 0.16
0.576CysPro: 0.576 ± 0.183
0.371CysGln: 0.371 ± 0.109
0.576CysArg: 0.576 ± 0.205
0.7CysSer: 0.7 ± 0.213
0.782CysThr: 0.782 ± 0.22
0.576CysVal: 0.576 ± 0.232
0.124CysTrp: 0.124 ± 0.076
0.329CysTyr: 0.329 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
4.94AspAla: 4.94 ± 0.447
0.659AspCys: 0.659 ± 0.17
4.24AspAsp: 4.24 ± 0.593
5.064AspGlu: 5.064 ± 0.557
2.141AspPhe: 2.141 ± 0.304
4.364AspGly: 4.364 ± 0.534
1.276AspHis: 1.276 ± 0.214
3.129AspIle: 3.129 ± 0.393
2.594AspLys: 2.594 ± 0.304
4.282AspLeu: 4.282 ± 0.487
1.935AspMet: 1.935 ± 0.249
2.511AspAsn: 2.511 ± 0.325
4.487AspPro: 4.487 ± 0.585
2.305AspGln: 2.305 ± 0.263
2.8AspArg: 2.8 ± 0.417
3.499AspSer: 3.499 ± 0.354
3.458AspThr: 3.458 ± 0.419
3.829AspVal: 3.829 ± 0.439
1.482AspTrp: 1.482 ± 0.263
2.635AspTyr: 2.635 ± 0.394
0.0AspXaa: 0.0 ± 0.0
Glu
6.093GluAla: 6.093 ± 0.697
1.153GluCys: 1.153 ± 0.313
4.117GluAsp: 4.117 ± 0.528
5.97GluGlu: 5.97 ± 0.669
2.841GluPhe: 2.841 ± 0.467
4.24GluGly: 4.24 ± 0.537
1.029GluHis: 1.029 ± 0.218
3.664GluIle: 3.664 ± 0.496
3.047GluLys: 3.047 ± 0.362
5.146GluLeu: 5.146 ± 0.566
2.017GluMet: 2.017 ± 0.365
1.811GluAsn: 1.811 ± 0.288
2.923GluPro: 2.923 ± 0.61
3.129GluGln: 3.129 ± 0.449
4.405GluArg: 4.405 ± 0.667
3.952GluSer: 3.952 ± 0.321
3.541GluThr: 3.541 ± 0.426
5.146GluVal: 5.146 ± 0.443
1.112GluTrp: 1.112 ± 0.229
2.1GluTyr: 2.1 ± 0.376
0.0GluXaa: 0.0 ± 0.0
Phe
3.088PheAla: 3.088 ± 0.408
0.576PheCys: 0.576 ± 0.144
2.882PheAsp: 2.882 ± 0.319
2.717PheGlu: 2.717 ± 0.43
1.029PhePhe: 1.029 ± 0.237
3.129PheGly: 3.129 ± 0.454
0.535PheHis: 0.535 ± 0.182
1.729PheIle: 1.729 ± 0.271
2.635PheLys: 2.635 ± 0.299
1.976PheLeu: 1.976 ± 0.33
1.153PheMet: 1.153 ± 0.201
1.523PheAsn: 1.523 ± 0.235
1.4PhePro: 1.4 ± 0.286
0.988PheGln: 0.988 ± 0.231
1.647PheArg: 1.647 ± 0.268
2.223PheSer: 2.223 ± 0.348
2.388PheThr: 2.388 ± 0.331
1.894PheVal: 1.894 ± 0.276
0.535PheTrp: 0.535 ± 0.172
0.741PheTyr: 0.741 ± 0.173
0.0PheXaa: 0.0 ± 0.0
Gly
7.122GlyAla: 7.122 ± 0.768
0.865GlyCys: 0.865 ± 0.202
4.652GlyAsp: 4.652 ± 0.456
4.364GlyGlu: 4.364 ± 0.423
2.964GlyPhe: 2.964 ± 0.337
7.781GlyGly: 7.781 ± 0.769
1.482GlyHis: 1.482 ± 0.281
4.24GlyIle: 4.24 ± 0.424
4.858GlyLys: 4.858 ± 0.382
5.599GlyLeu: 5.599 ± 0.559
2.305GlyMet: 2.305 ± 0.31
3.458GlyAsn: 3.458 ± 0.469
3.582GlyPro: 3.582 ± 0.307
3.17GlyGln: 3.17 ± 0.39
3.952GlyArg: 3.952 ± 0.475
5.311GlySer: 5.311 ± 0.642
5.352GlyThr: 5.352 ± 0.464
4.981GlyVal: 4.981 ± 0.408
1.523GlyTrp: 1.523 ± 0.282
2.594GlyTyr: 2.594 ± 0.46
0.0GlyXaa: 0.0 ± 0.0
His
1.359HisAla: 1.359 ± 0.284
0.124HisCys: 0.124 ± 0.068
1.029HisAsp: 1.029 ± 0.289
1.153HisGlu: 1.153 ± 0.199
0.453HisPhe: 0.453 ± 0.151
1.153HisGly: 1.153 ± 0.214
0.371HisHis: 0.371 ± 0.168
0.906HisIle: 0.906 ± 0.234
0.659HisLys: 0.659 ± 0.2
1.523HisLeu: 1.523 ± 0.291
0.618HisMet: 0.618 ± 0.153
0.576HisAsn: 0.576 ± 0.177
1.317HisPro: 1.317 ± 0.254
0.535HisGln: 0.535 ± 0.14
1.317HisArg: 1.317 ± 0.262
0.947HisSer: 0.947 ± 0.205
1.112HisThr: 1.112 ± 0.227
1.112HisVal: 1.112 ± 0.208
0.206HisTrp: 0.206 ± 0.094
0.741HisTyr: 0.741 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
5.146IleAla: 5.146 ± 0.514
0.329IleCys: 0.329 ± 0.109
3.746IleAsp: 3.746 ± 0.433
4.364IleGlu: 4.364 ± 0.538
1.894IlePhe: 1.894 ± 0.321
5.517IleGly: 5.517 ± 0.554
0.659IleHis: 0.659 ± 0.165
2.8IleIle: 2.8 ± 0.429
2.8IleLys: 2.8 ± 0.34
2.882IleLeu: 2.882 ± 0.296
1.441IleMet: 1.441 ± 0.251
2.141IleAsn: 2.141 ± 0.293
2.182IlePro: 2.182 ± 0.304
2.429IleGln: 2.429 ± 0.335
2.594IleArg: 2.594 ± 0.393
3.17IleSer: 3.17 ± 0.392
3.252IleThr: 3.252 ± 0.314
3.911IleVal: 3.911 ± 0.444
0.865IleTrp: 0.865 ± 0.244
1.359IleTyr: 1.359 ± 0.241
0.0IleXaa: 0.0 ± 0.0
Lys
4.734LysAla: 4.734 ± 0.501
0.535LysCys: 0.535 ± 0.178
2.388LysAsp: 2.388 ± 0.474
3.458LysGlu: 3.458 ± 0.384
2.264LysPhe: 2.264 ± 0.339
3.993LysGly: 3.993 ± 0.391
0.7LysHis: 0.7 ± 0.222
2.758LysIle: 2.758 ± 0.31
3.252LysLys: 3.252 ± 0.486
4.94LysLeu: 4.94 ± 0.47
1.564LysMet: 1.564 ± 0.242
2.429LysAsn: 2.429 ± 0.326
2.594LysPro: 2.594 ± 0.389
2.388LysGln: 2.388 ± 0.23
3.582LysArg: 3.582 ± 0.459
2.758LysSer: 2.758 ± 0.391
2.305LysThr: 2.305 ± 0.32
3.87LysVal: 3.87 ± 0.417
0.741LysTrp: 0.741 ± 0.163
1.606LysTyr: 1.606 ± 0.277
0.0LysXaa: 0.0 ± 0.0
Leu
6.711LeuAla: 6.711 ± 0.564
0.741LeuCys: 0.741 ± 0.193
4.94LeuAsp: 4.94 ± 0.446
5.681LeuGlu: 5.681 ± 0.485
2.676LeuPhe: 2.676 ± 0.323
5.558LeuGly: 5.558 ± 0.441
1.235LeuHis: 1.235 ± 0.215
3.829LeuIle: 3.829 ± 0.369
3.788LeuLys: 3.788 ± 0.482
5.146LeuLeu: 5.146 ± 0.427
2.429LeuMet: 2.429 ± 0.295
2.758LeuAsn: 2.758 ± 0.379
3.746LeuPro: 3.746 ± 0.427
2.635LeuGln: 2.635 ± 0.255
4.57LeuArg: 4.57 ± 0.506
4.652LeuSer: 4.652 ± 0.476
4.611LeuThr: 4.611 ± 0.417
4.529LeuVal: 4.529 ± 0.425
0.988LeuTrp: 0.988 ± 0.195
1.606LeuTyr: 1.606 ± 0.294
0.0LeuXaa: 0.0 ± 0.0
Met
2.388MetAla: 2.388 ± 0.383
0.288MetCys: 0.288 ± 0.09
1.276MetAsp: 1.276 ± 0.276
1.482MetGlu: 1.482 ± 0.252
0.988MetPhe: 0.988 ± 0.207
1.894MetGly: 1.894 ± 0.274
0.453MetHis: 0.453 ± 0.167
1.029MetIle: 1.029 ± 0.18
1.688MetLys: 1.688 ± 0.269
2.223MetLeu: 2.223 ± 0.28
0.453MetMet: 0.453 ± 0.153
1.194MetAsn: 1.194 ± 0.19
2.1MetPro: 2.1 ± 0.256
0.865MetGln: 0.865 ± 0.205
1.482MetArg: 1.482 ± 0.26
2.305MetSer: 2.305 ± 0.299
2.264MetThr: 2.264 ± 0.277
1.894MetVal: 1.894 ± 0.37
0.329MetTrp: 0.329 ± 0.122
0.906MetTyr: 0.906 ± 0.185
0.0MetXaa: 0.0 ± 0.0
Asn
4.446AsnAla: 4.446 ± 0.589
0.124AsnCys: 0.124 ± 0.07
2.676AsnAsp: 2.676 ± 0.359
2.058AsnGlu: 2.058 ± 0.266
1.112AsnPhe: 1.112 ± 0.263
3.829AsnGly: 3.829 ± 0.362
0.741AsnHis: 0.741 ± 0.186
2.264AsnIle: 2.264 ± 0.299
2.305AsnLys: 2.305 ± 0.339
3.335AsnLeu: 3.335 ± 0.391
0.947AsnMet: 0.947 ± 0.209
2.017AsnAsn: 2.017 ± 0.348
2.923AsnPro: 2.923 ± 0.326
1.77AsnGln: 1.77 ± 0.257
1.811AsnArg: 1.811 ± 0.276
2.305AsnSer: 2.305 ± 0.31
1.729AsnThr: 1.729 ± 0.264
2.964AsnVal: 2.964 ± 0.313
0.618AsnTrp: 0.618 ± 0.188
1.029AsnTyr: 1.029 ± 0.178
0.0AsnXaa: 0.0 ± 0.0
Pro
5.558ProAla: 5.558 ± 0.511
0.288ProCys: 0.288 ± 0.126
4.076ProAsp: 4.076 ± 0.508
4.117ProGlu: 4.117 ± 0.641
1.564ProPhe: 1.564 ± 0.237
4.282ProGly: 4.282 ± 0.452
0.618ProHis: 0.618 ± 0.178
2.47ProIle: 2.47 ± 0.327
2.347ProLys: 2.347 ± 0.408
3.005ProLeu: 3.005 ± 0.459
1.194ProMet: 1.194 ± 0.214
2.347ProAsn: 2.347 ± 0.35
3.417ProPro: 3.417 ± 0.543
2.058ProGln: 2.058 ± 0.353
1.976ProArg: 1.976 ± 0.381
3.211ProSer: 3.211 ± 0.445
3.87ProThr: 3.87 ± 0.588
3.952ProVal: 3.952 ± 0.423
1.153ProTrp: 1.153 ± 0.203
2.017ProTyr: 2.017 ± 0.252
0.0ProXaa: 0.0 ± 0.0
Gln
3.993GlnAla: 3.993 ± 0.816
0.288GlnCys: 0.288 ± 0.117
1.523GlnAsp: 1.523 ± 0.289
2.1GlnGlu: 2.1 ± 0.317
1.482GlnPhe: 1.482 ± 0.248
2.717GlnGly: 2.717 ± 0.338
0.823GlnHis: 0.823 ± 0.23
2.305GlnIle: 2.305 ± 0.417
2.1GlnLys: 2.1 ± 0.293
3.047GlnLeu: 3.047 ± 0.382
1.029GlnMet: 1.029 ± 0.186
2.017GlnAsn: 2.017 ± 0.291
1.976GlnPro: 1.976 ± 0.393
1.853GlnGln: 1.853 ± 0.286
2.594GlnArg: 2.594 ± 0.331
1.935GlnSer: 1.935 ± 0.315
2.305GlnThr: 2.305 ± 0.334
2.758GlnVal: 2.758 ± 0.35
0.741GlnTrp: 0.741 ± 0.2
1.153GlnTyr: 1.153 ± 0.248
0.0GlnXaa: 0.0 ± 0.0
Arg
4.734ArgAla: 4.734 ± 0.473
0.7ArgCys: 0.7 ± 0.179
2.758ArgAsp: 2.758 ± 0.402
4.364ArgGlu: 4.364 ± 0.486
1.811ArgPhe: 1.811 ± 0.252
3.664ArgGly: 3.664 ± 0.403
1.235ArgHis: 1.235 ± 0.287
2.882ArgIle: 2.882 ± 0.364
3.499ArgLys: 3.499 ± 0.404
4.529ArgLeu: 4.529 ± 0.524
1.688ArgMet: 1.688 ± 0.269
2.676ArgAsn: 2.676 ± 0.362
2.594ArgPro: 2.594 ± 0.418
1.935ArgGln: 1.935 ± 0.304
3.705ArgArg: 3.705 ± 0.595
2.223ArgSer: 2.223 ± 0.323
2.717ArgThr: 2.717 ± 0.414
3.582ArgVal: 3.582 ± 0.494
0.823ArgTrp: 0.823 ± 0.194
1.935ArgTyr: 1.935 ± 0.29
0.0ArgXaa: 0.0 ± 0.0
Ser
6.258SerAla: 6.258 ± 0.603
0.494SerCys: 0.494 ± 0.168
3.17SerAsp: 3.17 ± 0.323
2.964SerGlu: 2.964 ± 0.464
2.182SerPhe: 2.182 ± 0.313
5.558SerGly: 5.558 ± 0.564
0.906SerHis: 0.906 ± 0.185
3.788SerIle: 3.788 ± 0.354
3.129SerLys: 3.129 ± 0.415
4.611SerLeu: 4.611 ± 0.476
1.359SerMet: 1.359 ± 0.268
2.388SerAsn: 2.388 ± 0.288
3.005SerPro: 3.005 ± 0.355
1.935SerGln: 1.935 ± 0.199
3.499SerArg: 3.499 ± 0.391
4.693SerSer: 4.693 ± 0.406
3.294SerThr: 3.294 ± 0.334
4.405SerVal: 4.405 ± 0.474
0.823SerTrp: 0.823 ± 0.208
1.976SerTyr: 1.976 ± 0.285
0.0SerXaa: 0.0 ± 0.0
Thr
5.723ThrAla: 5.723 ± 0.791
0.7ThrCys: 0.7 ± 0.221
4.24ThrAsp: 4.24 ± 0.455
3.088ThrGlu: 3.088 ± 0.409
2.305ThrPhe: 2.305 ± 0.282
5.023ThrGly: 5.023 ± 0.497
1.029ThrHis: 1.029 ± 0.223
3.458ThrIle: 3.458 ± 0.387
2.841ThrLys: 2.841 ± 0.367
4.405ThrLeu: 4.405 ± 0.383
1.153ThrMet: 1.153 ± 0.23
2.347ThrAsn: 2.347 ± 0.316
4.24ThrPro: 4.24 ± 0.67
2.017ThrGln: 2.017 ± 0.278
2.388ThrArg: 2.388 ± 0.323
3.623ThrSer: 3.623 ± 0.408
4.405ThrThr: 4.405 ± 0.488
4.693ThrVal: 4.693 ± 0.48
1.482ThrTrp: 1.482 ± 0.252
1.976ThrTyr: 1.976 ± 0.384
0.0ThrXaa: 0.0 ± 0.0
Val
6.052ValAla: 6.052 ± 0.698
0.618ValCys: 0.618 ± 0.195
4.57ValAsp: 4.57 ± 0.444
4.652ValGlu: 4.652 ± 0.419
2.47ValPhe: 2.47 ± 0.345
4.94ValGly: 4.94 ± 0.47
1.647ValHis: 1.647 ± 0.277
3.993ValIle: 3.993 ± 0.468
3.623ValLys: 3.623 ± 0.345
4.282ValLeu: 4.282 ± 0.362
2.141ValMet: 2.141 ± 0.311
2.8ValAsn: 2.8 ± 0.362
3.499ValPro: 3.499 ± 0.422
2.182ValGln: 2.182 ± 0.33
3.499ValArg: 3.499 ± 0.456
4.076ValSer: 4.076 ± 0.569
5.105ValThr: 5.105 ± 0.366
4.734ValVal: 4.734 ± 0.388
1.441ValTrp: 1.441 ± 0.238
1.729ValTyr: 1.729 ± 0.24
0.0ValXaa: 0.0 ± 0.0
Trp
1.4TrpAla: 1.4 ± 0.422
0.329TrpCys: 0.329 ± 0.115
1.441TrpAsp: 1.441 ± 0.267
1.276TrpGlu: 1.276 ± 0.22
0.535TrpPhe: 0.535 ± 0.152
1.235TrpGly: 1.235 ± 0.214
0.329TrpHis: 0.329 ± 0.15
0.988TrpIle: 0.988 ± 0.203
1.029TrpLys: 1.029 ± 0.199
1.482TrpLeu: 1.482 ± 0.25
0.288TrpMet: 0.288 ± 0.098
0.741TrpAsn: 0.741 ± 0.216
0.865TrpPro: 0.865 ± 0.198
0.535TrpGln: 0.535 ± 0.127
1.029TrpArg: 1.029 ± 0.238
1.112TrpSer: 1.112 ± 0.225
0.947TrpThr: 0.947 ± 0.218
1.07TrpVal: 1.07 ± 0.239
0.247TrpTrp: 0.247 ± 0.105
0.782TrpTyr: 0.782 ± 0.185
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.676TyrAla: 2.676 ± 0.356
0.535TyrCys: 0.535 ± 0.182
1.894TyrAsp: 1.894 ± 0.288
1.688TyrGlu: 1.688 ± 0.29
1.112TyrPhe: 1.112 ± 0.214
2.882TyrGly: 2.882 ± 0.374
0.741TyrHis: 0.741 ± 0.168
1.606TyrIle: 1.606 ± 0.287
1.647TyrLys: 1.647 ± 0.249
2.47TyrLeu: 2.47 ± 0.361
0.906TyrMet: 0.906 ± 0.21
1.317TyrAsn: 1.317 ± 0.229
1.4TyrPro: 1.4 ± 0.267
1.359TyrGln: 1.359 ± 0.271
1.77TyrArg: 1.77 ± 0.323
1.4TyrSer: 1.4 ± 0.236
1.976TyrThr: 1.976 ± 0.324
2.182TyrVal: 2.182 ± 0.349
0.535TyrTrp: 0.535 ± 0.14
0.947TyrTyr: 0.947 ± 0.207
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 109 proteins (24291 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski