Amino acid dipepetide frequency for Escherichia phage Rac

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.644AlaAla: 8.644 ± 1.689
0.455AlaCys: 0.455 ± 0.441
5.005AlaAsp: 5.005 ± 1.28
5.914AlaGlu: 5.914 ± 1.202
3.64AlaPhe: 3.64 ± 1.433
5.005AlaGly: 5.005 ± 1.303
2.275AlaHis: 2.275 ± 1.033
8.644AlaIle: 8.644 ± 2.157
4.55AlaLys: 4.55 ± 2.163
13.194AlaLeu: 13.194 ± 3.011
3.64AlaMet: 3.64 ± 1.231
2.73AlaAsn: 2.73 ± 1.15
2.73AlaPro: 2.73 ± 0.98
3.64AlaGln: 3.64 ± 1.146
4.095AlaArg: 4.095 ± 1.881
5.914AlaSer: 5.914 ± 1.907
3.64AlaThr: 3.64 ± 1.17
3.185AlaVal: 3.185 ± 1.286
1.365AlaTrp: 1.365 ± 0.729
0.91AlaTyr: 0.91 ± 0.651
0.0AlaXaa: 0.0 ± 0.0
Cys
0.91CysAla: 0.91 ± 0.698
0.0CysCys: 0.0 ± 0.0
1.82CysAsp: 1.82 ± 1.037
2.275CysGlu: 2.275 ± 1.012
0.0CysPhe: 0.0 ± 0.0
0.91CysGly: 0.91 ± 0.709
0.455CysHis: 0.455 ± 0.441
0.91CysIle: 0.91 ± 0.596
0.0CysLys: 0.0 ± 0.0
1.365CysLeu: 1.365 ± 0.662
0.91CysMet: 0.91 ± 0.59
1.365CysAsn: 1.365 ± 0.951
0.0CysPro: 0.0 ± 0.0
0.455CysGln: 0.455 ± 0.464
1.365CysArg: 1.365 ± 1.074
2.275CysSer: 2.275 ± 0.831
0.91CysThr: 0.91 ± 0.574
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.455CysTyr: 0.455 ± 0.423
0.0CysXaa: 0.0 ± 0.0
Asp
6.369AspAla: 6.369 ± 1.666
0.0AspCys: 0.0 ± 0.0
3.185AspAsp: 3.185 ± 1.22
6.369AspGlu: 6.369 ± 1.848
1.82AspPhe: 1.82 ± 0.838
6.369AspGly: 6.369 ± 1.453
0.0AspHis: 0.0 ± 0.0
1.365AspIle: 1.365 ± 0.884
1.365AspLys: 1.365 ± 0.643
3.64AspLeu: 3.64 ± 1.302
0.91AspMet: 0.91 ± 0.605
0.455AspAsn: 0.455 ± 0.406
1.365AspPro: 1.365 ± 0.666
0.91AspGln: 0.91 ± 0.771
2.275AspArg: 2.275 ± 0.931
1.82AspSer: 1.82 ± 0.708
2.73AspThr: 2.73 ± 0.887
1.82AspVal: 1.82 ± 0.969
1.365AspTrp: 1.365 ± 0.653
2.73AspTyr: 2.73 ± 1.205
0.0AspXaa: 0.0 ± 0.0
Glu
9.554GluAla: 9.554 ± 1.828
0.91GluCys: 0.91 ± 0.539
2.275GluAsp: 2.275 ± 1.104
6.824GluGlu: 6.824 ± 1.715
2.275GluPhe: 2.275 ± 1.252
2.275GluGly: 2.275 ± 1.105
2.275GluHis: 2.275 ± 0.901
7.279GluIle: 7.279 ± 1.773
5.005GluLys: 5.005 ± 1.499
10.919GluLeu: 10.919 ± 2.657
1.82GluMet: 1.82 ± 1.243
2.73GluAsn: 2.73 ± 1.07
1.82GluPro: 1.82 ± 0.731
4.55GluGln: 4.55 ± 1.117
4.55GluArg: 4.55 ± 0.761
3.185GluSer: 3.185 ± 0.889
4.55GluThr: 4.55 ± 0.945
4.55GluVal: 4.55 ± 1.24
4.095GluTrp: 4.095 ± 1.331
1.82GluTyr: 1.82 ± 0.854
0.0GluXaa: 0.0 ± 0.0
Phe
3.64PheAla: 3.64 ± 1.145
0.0PheCys: 0.0 ± 0.0
2.275PheAsp: 2.275 ± 0.948
3.64PheGlu: 3.64 ± 1.374
1.365PhePhe: 1.365 ± 0.72
1.365PheGly: 1.365 ± 0.782
0.455PheHis: 0.455 ± 0.407
0.0PheIle: 0.0 ± 0.0
2.275PheLys: 2.275 ± 0.998
1.365PheLeu: 1.365 ± 0.613
0.91PheMet: 0.91 ± 0.595
3.185PheAsn: 3.185 ± 1.181
0.91PhePro: 0.91 ± 0.601
0.455PheGln: 0.455 ± 0.407
5.005PheArg: 5.005 ± 1.581
2.73PheSer: 2.73 ± 0.995
1.365PheThr: 1.365 ± 0.715
2.275PheVal: 2.275 ± 1.296
0.455PheTrp: 0.455 ± 0.386
1.82PheTyr: 1.82 ± 0.991
0.0PheXaa: 0.0 ± 0.0
Gly
2.73GlyAla: 2.73 ± 1.002
0.91GlyCys: 0.91 ± 0.527
4.095GlyAsp: 4.095 ± 1.325
6.824GlyGlu: 6.824 ± 1.619
4.095GlyPhe: 4.095 ± 1.808
5.914GlyGly: 5.914 ± 1.963
1.82GlyHis: 1.82 ± 0.763
2.73GlyIle: 2.73 ± 1.461
6.824GlyLys: 6.824 ± 1.711
2.73GlyLeu: 2.73 ± 1.076
2.275GlyMet: 2.275 ± 0.664
3.185GlyAsn: 3.185 ± 0.795
0.91GlyPro: 0.91 ± 0.812
4.55GlyGln: 4.55 ± 2.18
5.005GlyArg: 5.005 ± 1.257
3.185GlySer: 3.185 ± 1.201
1.82GlyThr: 1.82 ± 1.057
4.55GlyVal: 4.55 ± 1.398
0.455GlyTrp: 0.455 ± 0.386
1.82GlyTyr: 1.82 ± 1.173
0.0GlyXaa: 0.0 ± 0.0
His
1.82HisAla: 1.82 ± 0.75
0.91HisCys: 0.91 ± 0.564
1.82HisAsp: 1.82 ± 1.084
1.365HisGlu: 1.365 ± 0.779
0.91HisPhe: 0.91 ± 0.529
1.365HisGly: 1.365 ± 0.684
0.0HisHis: 0.0 ± 0.0
0.91HisIle: 0.91 ± 0.529
0.91HisLys: 0.91 ± 0.643
2.275HisLeu: 2.275 ± 0.947
1.365HisMet: 1.365 ± 0.77
1.365HisAsn: 1.365 ± 0.742
0.455HisPro: 0.455 ± 0.397
1.365HisGln: 1.365 ± 0.71
1.365HisArg: 1.365 ± 0.906
0.91HisSer: 0.91 ± 0.515
1.365HisThr: 1.365 ± 0.835
2.275HisVal: 2.275 ± 0.996
0.0HisTrp: 0.0 ± 0.0
0.455HisTyr: 0.455 ± 0.441
0.0HisXaa: 0.0 ± 0.0
Ile
5.005IleAla: 5.005 ± 1.318
1.365IleCys: 1.365 ± 1.011
3.185IleAsp: 3.185 ± 1.312
3.64IleGlu: 3.64 ± 0.906
2.73IlePhe: 2.73 ± 0.952
3.64IleGly: 3.64 ± 1.763
1.82IleHis: 1.82 ± 0.769
1.82IleIle: 1.82 ± 0.796
3.64IleLys: 3.64 ± 1.108
4.095IleLeu: 4.095 ± 1.011
0.455IleMet: 0.455 ± 0.426
2.275IleAsn: 2.275 ± 0.914
1.82IlePro: 1.82 ± 0.835
2.73IleGln: 2.73 ± 0.829
6.824IleArg: 6.824 ± 1.576
2.275IleSer: 2.275 ± 0.724
4.095IleThr: 4.095 ± 1.287
4.095IleVal: 4.095 ± 1.739
0.455IleTrp: 0.455 ± 0.441
2.73IleTyr: 2.73 ± 1.321
0.0IleXaa: 0.0 ± 0.0
Lys
5.914LysAla: 5.914 ± 1.444
0.91LysCys: 0.91 ± 0.599
2.275LysAsp: 2.275 ± 0.862
3.185LysGlu: 3.185 ± 1.502
2.275LysPhe: 2.275 ± 0.655
6.824LysGly: 6.824 ± 1.81
1.365LysHis: 1.365 ± 0.702
4.55LysIle: 4.55 ± 0.957
1.82LysLys: 1.82 ± 0.765
4.55LysLeu: 4.55 ± 0.984
2.275LysMet: 2.275 ± 0.798
2.275LysAsn: 2.275 ± 1.358
1.82LysPro: 1.82 ± 0.823
0.91LysGln: 0.91 ± 0.617
5.005LysArg: 5.005 ± 1.234
5.914LysSer: 5.914 ± 1.699
4.095LysThr: 4.095 ± 1.118
3.185LysVal: 3.185 ± 1.174
0.91LysTrp: 0.91 ± 0.585
1.365LysTyr: 1.365 ± 0.844
0.0LysXaa: 0.0 ± 0.0
Leu
10.464LeuAla: 10.464 ± 2.774
2.73LeuCys: 2.73 ± 0.991
0.91LeuAsp: 0.91 ± 0.649
4.095LeuGlu: 4.095 ± 1.283
3.64LeuPhe: 3.64 ± 1.6
2.73LeuGly: 2.73 ± 0.615
3.185LeuHis: 3.185 ± 1.381
7.279LeuIle: 7.279 ± 1.724
5.005LeuLys: 5.005 ± 1.327
8.644LeuLeu: 8.644 ± 2.035
2.73LeuMet: 2.73 ± 1.177
5.005LeuAsn: 5.005 ± 0.933
7.279LeuPro: 7.279 ± 2.764
2.275LeuGln: 2.275 ± 0.983
11.374LeuArg: 11.374 ± 2.012
2.73LeuSer: 2.73 ± 0.694
3.185LeuThr: 3.185 ± 1.182
5.005LeuVal: 5.005 ± 1.101
1.365LeuTrp: 1.365 ± 0.631
0.91LeuTyr: 0.91 ± 0.564
0.0LeuXaa: 0.0 ± 0.0
Met
4.095MetAla: 4.095 ± 1.159
0.455MetCys: 0.455 ± 0.441
1.82MetAsp: 1.82 ± 0.79
0.91MetGlu: 0.91 ± 0.57
1.365MetPhe: 1.365 ± 0.692
0.455MetGly: 0.455 ± 0.406
0.455MetHis: 0.455 ± 0.441
0.91MetIle: 0.91 ± 0.535
2.275MetLys: 2.275 ± 1.043
5.005MetLeu: 5.005 ± 1.649
0.0MetMet: 0.0 ± 0.0
0.91MetAsn: 0.91 ± 0.649
0.455MetPro: 0.455 ± 0.503
0.91MetGln: 0.91 ± 0.709
1.365MetArg: 1.365 ± 0.692
1.82MetSer: 1.82 ± 1.028
1.365MetThr: 1.365 ± 0.644
1.365MetVal: 1.365 ± 0.78
0.91MetTrp: 0.91 ± 0.535
0.455MetTyr: 0.455 ± 0.563
0.0MetXaa: 0.0 ± 0.0
Asn
3.185AsnAla: 3.185 ± 1.238
0.0AsnCys: 0.0 ± 0.0
0.91AsnAsp: 0.91 ± 0.539
4.55AsnGlu: 4.55 ± 1.168
2.73AsnPhe: 2.73 ± 0.8
4.095AsnGly: 4.095 ± 1.463
0.91AsnHis: 0.91 ± 0.574
3.185AsnIle: 3.185 ± 0.852
2.275AsnLys: 2.275 ± 1.061
0.91AsnLeu: 0.91 ± 0.539
1.365AsnMet: 1.365 ± 0.675
0.455AsnAsn: 0.455 ± 0.435
1.365AsnPro: 1.365 ± 0.677
1.82AsnGln: 1.82 ± 0.848
4.55AsnArg: 4.55 ± 2.049
1.365AsnSer: 1.365 ± 0.75
1.82AsnThr: 1.82 ± 0.914
1.82AsnVal: 1.82 ± 0.953
1.82AsnTrp: 1.82 ± 0.605
1.82AsnTyr: 1.82 ± 0.985
0.0AsnXaa: 0.0 ± 0.0
Pro
3.185ProAla: 3.185 ± 1.166
0.0ProCys: 0.0 ± 0.0
5.005ProAsp: 5.005 ± 1.635
5.914ProGlu: 5.914 ± 2.338
0.455ProPhe: 0.455 ± 0.426
3.185ProGly: 3.185 ± 0.881
0.455ProHis: 0.455 ± 0.441
0.455ProIle: 0.455 ± 0.386
2.275ProLys: 2.275 ± 0.924
2.275ProLeu: 2.275 ± 0.866
0.91ProMet: 0.91 ± 0.535
1.365ProAsn: 1.365 ± 0.581
0.91ProPro: 0.91 ± 0.771
0.455ProGln: 0.455 ± 0.406
0.91ProArg: 0.91 ± 0.586
3.64ProSer: 3.64 ± 1.144
0.91ProThr: 0.91 ± 0.563
2.275ProVal: 2.275 ± 0.831
0.0ProTrp: 0.0 ± 0.0
0.0ProTyr: 0.0 ± 0.0
0.0ProXaa: 0.0 ± 0.0
Gln
4.095GlnAla: 4.095 ± 1.097
0.0GlnCys: 0.0 ± 0.0
0.455GlnAsp: 0.455 ± 0.406
5.005GlnGlu: 5.005 ± 1.633
1.365GlnPhe: 1.365 ± 0.843
3.185GlnGly: 3.185 ± 1.229
1.365GlnHis: 1.365 ± 0.712
1.82GlnIle: 1.82 ± 0.73
3.64GlnLys: 3.64 ± 1.02
3.64GlnLeu: 3.64 ± 1.373
1.365GlnMet: 1.365 ± 0.884
3.185GlnAsn: 3.185 ± 1.249
2.275GlnPro: 2.275 ± 0.955
2.73GlnGln: 2.73 ± 1.137
3.185GlnArg: 3.185 ± 1.181
1.365GlnSer: 1.365 ± 0.677
1.82GlnThr: 1.82 ± 0.711
2.73GlnVal: 2.73 ± 1.158
0.455GlnTrp: 0.455 ± 0.474
0.91GlnTyr: 0.91 ± 0.569
0.0GlnXaa: 0.0 ± 0.0
Arg
5.46ArgAla: 5.46 ± 1.303
1.365ArgCys: 1.365 ± 0.974
1.365ArgAsp: 1.365 ± 0.656
9.099ArgGlu: 9.099 ± 1.52
0.91ArgPhe: 0.91 ± 0.539
3.185ArgGly: 3.185 ± 1.026
2.275ArgHis: 2.275 ± 1.228
4.55ArgIle: 4.55 ± 1.069
8.189ArgLys: 8.189 ± 1.863
7.734ArgLeu: 7.734 ± 1.934
1.365ArgMet: 1.365 ± 0.761
1.365ArgAsn: 1.365 ± 0.633
2.73ArgPro: 2.73 ± 1.726
5.914ArgGln: 5.914 ± 1.78
5.914ArgArg: 5.914 ± 1.725
2.73ArgSer: 2.73 ± 0.961
3.64ArgThr: 3.64 ± 1.055
4.55ArgVal: 4.55 ± 1.09
1.365ArgTrp: 1.365 ± 0.654
3.185ArgTyr: 3.185 ± 1.207
0.0ArgXaa: 0.0 ± 0.0
Ser
3.185SerAla: 3.185 ± 1.048
1.365SerCys: 1.365 ± 0.703
1.82SerAsp: 1.82 ± 0.702
4.095SerGlu: 4.095 ± 1.47
2.275SerPhe: 2.275 ± 0.857
6.369SerGly: 6.369 ± 1.939
2.275SerHis: 2.275 ± 0.807
3.185SerIle: 3.185 ± 0.92
3.64SerLys: 3.64 ± 1.074
4.095SerLeu: 4.095 ± 1.38
0.91SerMet: 0.91 ± 0.794
1.82SerAsn: 1.82 ± 0.646
0.91SerPro: 0.91 ± 0.643
1.82SerGln: 1.82 ± 0.831
3.185SerArg: 3.185 ± 1.144
5.46SerSer: 5.46 ± 1.862
1.365SerThr: 1.365 ± 0.826
5.46SerVal: 5.46 ± 1.308
0.91SerTrp: 0.91 ± 0.584
1.365SerTyr: 1.365 ± 0.724
0.0SerXaa: 0.0 ± 0.0
Thr
3.185ThrAla: 3.185 ± 1.283
1.365ThrCys: 1.365 ± 0.633
2.275ThrAsp: 2.275 ± 1.157
3.185ThrGlu: 3.185 ± 0.999
0.455ThrPhe: 0.455 ± 0.386
3.64ThrGly: 3.64 ± 1.687
0.0ThrHis: 0.0 ± 0.0
1.82ThrIle: 1.82 ± 1.102
4.55ThrLys: 4.55 ± 1.147
3.64ThrLeu: 3.64 ± 0.914
1.365ThrMet: 1.365 ± 0.711
2.275ThrAsn: 2.275 ± 0.951
4.55ThrPro: 4.55 ± 1.689
3.185ThrGln: 3.185 ± 1.091
2.73ThrArg: 2.73 ± 1.055
3.64ThrSer: 3.64 ± 1.532
0.0ThrThr: 0.0 ± 0.0
4.095ThrVal: 4.095 ± 1.291
0.455ThrTrp: 0.455 ± 0.474
0.0ThrTyr: 0.0 ± 0.0
0.0ThrXaa: 0.0 ± 0.0
Val
4.55ValAla: 4.55 ± 1.473
1.365ValCys: 1.365 ± 0.831
4.55ValAsp: 4.55 ± 1.386
2.73ValGlu: 2.73 ± 1.136
2.73ValPhe: 2.73 ± 1.035
4.095ValGly: 4.095 ± 1.307
0.91ValHis: 0.91 ± 0.549
3.64ValIle: 3.64 ± 1.187
1.365ValLys: 1.365 ± 0.772
5.005ValLeu: 5.005 ± 1.458
1.82ValMet: 1.82 ± 1.085
2.73ValAsn: 2.73 ± 0.868
0.455ValPro: 0.455 ± 0.46
2.73ValGln: 2.73 ± 1.259
4.095ValArg: 4.095 ± 1.014
3.64ValSer: 3.64 ± 1.272
5.914ValThr: 5.914 ± 1.937
4.55ValVal: 4.55 ± 1.758
0.91ValTrp: 0.91 ± 0.595
1.82ValTyr: 1.82 ± 0.771
0.0ValXaa: 0.0 ± 0.0
Trp
0.91TrpAla: 0.91 ± 0.771
0.455TrpCys: 0.455 ± 0.397
0.0TrpAsp: 0.0 ± 0.0
1.365TrpGlu: 1.365 ± 0.6
0.0TrpPhe: 0.0 ± 0.0
1.365TrpGly: 1.365 ± 0.727
0.455TrpHis: 0.455 ± 0.467
1.82TrpIle: 1.82 ± 0.881
1.365TrpLys: 1.365 ± 0.831
3.185TrpLeu: 3.185 ± 1.096
0.0TrpMet: 0.0 ± 0.0
1.365TrpAsn: 1.365 ± 0.639
0.0TrpPro: 0.0 ± 0.0
0.91TrpGln: 0.91 ± 0.623
1.82TrpArg: 1.82 ± 1.016
0.455TrpSer: 0.455 ± 0.397
0.455TrpThr: 0.455 ± 0.407
1.82TrpVal: 1.82 ± 0.591
0.455TrpTrp: 0.455 ± 0.406
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.275TyrAla: 2.275 ± 0.818
1.82TyrCys: 1.82 ± 0.774
1.82TyrAsp: 1.82 ± 1.066
2.275TyrGlu: 2.275 ± 0.913
0.455TyrPhe: 0.455 ± 0.563
0.455TyrGly: 0.455 ± 0.485
0.455TyrHis: 0.455 ± 0.435
1.82TyrIle: 1.82 ± 1.042
0.455TyrLys: 0.455 ± 0.407
2.73TyrLeu: 2.73 ± 1.101
0.455TyrMet: 0.455 ± 0.472
0.91TyrAsn: 0.91 ± 0.658
1.82TyrPro: 1.82 ± 0.904
2.275TyrGln: 2.275 ± 0.712
2.275TyrArg: 2.275 ± 0.925
0.455TyrSer: 0.455 ± 0.563
1.365TyrThr: 1.365 ± 0.754
0.0TyrVal: 0.0 ± 0.0
0.455TyrTrp: 0.455 ± 0.407
0.0TyrTyr: 0.0 ± 0.0
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 17 proteins (2199 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski