Leptolyngbya boryana NIES-2135

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Leptolyngbyaceae; Leptolyngbya; Leptolyngbya boryana

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6678 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z4JQA1|A0A1Z4JQA1_LEPBY Cell wall hydrolase/autolysin OS=Leptolyngbya boryana NIES-2135 OX=1973484 GN=NIES2135_57640 PE=4 SV=1
MM1 pKa = 7.09ATVGLFYY8 pKa = 10.15GTQTGNTEE16 pKa = 4.45TIAEE20 pKa = 5.17AIQAQLGGDD29 pKa = 4.15SIVEE33 pKa = 3.94LSDD36 pKa = 2.99IANASSEE43 pKa = 4.11DD44 pKa = 3.79FEE46 pKa = 4.87PYY48 pKa = 9.72EE49 pKa = 4.15YY50 pKa = 10.78LIIGCPTWNIGEE62 pKa = 4.5LQADD66 pKa = 3.44WEE68 pKa = 4.51GFYY71 pKa = 11.3DD72 pKa = 4.33EE73 pKa = 5.75LDD75 pKa = 3.83NINFNGKK82 pKa = 8.53KK83 pKa = 9.33VAYY86 pKa = 8.86FGCGDD91 pKa = 3.67QIGYY95 pKa = 10.75ADD97 pKa = 4.11NFQDD101 pKa = 4.12AMGILEE107 pKa = 4.46EE108 pKa = 5.13KK109 pKa = 10.48ISGLGGQTVGMTSTAGYY126 pKa = 8.88EE127 pKa = 4.21HH128 pKa = 6.69QEE130 pKa = 4.17SKK132 pKa = 10.97AVRR135 pKa = 11.84GDD137 pKa = 3.64KK138 pKa = 10.74FCGLAIDD145 pKa = 5.14EE146 pKa = 5.32DD147 pKa = 4.18NQSDD151 pKa = 4.0LTDD154 pKa = 3.49EE155 pKa = 5.26RR156 pKa = 11.84IKK158 pKa = 10.99AWATQLKK165 pKa = 9.89SAFGII170 pKa = 4.18

Molecular weight: 18.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z4JMH1|A0A1Z4JMH1_LEPBY Uncharacterized protein OS=Leptolyngbya boryana NIES-2135 OX=1973484 GN=NIES2135_47190 PE=4 SV=1
MM1 pKa = 8.04LDD3 pKa = 4.08LLLMASVGFLGSFGHH18 pKa = 6.47CLGMCSPLTVAFSLSNDD35 pKa = 3.19RR36 pKa = 11.84TSRR39 pKa = 11.84FAQLRR44 pKa = 11.84FNLLLNAGRR53 pKa = 11.84VMSYY57 pKa = 9.08TLVGAAIGALGSVLIAGGQLAGIDD81 pKa = 3.46SALRR85 pKa = 11.84RR86 pKa = 11.84GLAIVTGLLLIWFGLANINPTWLPQVPILHH116 pKa = 7.16PIQGKK121 pKa = 8.55LHH123 pKa = 6.84DD124 pKa = 4.86RR125 pKa = 11.84LSQRR129 pKa = 11.84LGDD132 pKa = 3.86FSRR135 pKa = 11.84SRR137 pKa = 11.84KK138 pKa = 6.53WWTPAILGLVWGLIPCGFLYY158 pKa = 10.61AAQIKK163 pKa = 9.76AAEE166 pKa = 4.4TGNALQGALTMLAFGGGTAPMMISTGLFAGALSRR200 pKa = 11.84DD201 pKa = 3.39RR202 pKa = 11.84RR203 pKa = 11.84SQLFKK208 pKa = 10.43LAGWITLTIGLLTLFRR224 pKa = 11.84SSDD227 pKa = 3.34MVDD230 pKa = 3.7YY231 pKa = 10.0TGHH234 pKa = 7.01AALICLMVALIARR247 pKa = 11.84PISRR251 pKa = 11.84IWAAPLRR258 pKa = 11.84YY259 pKa = 9.12RR260 pKa = 11.84RR261 pKa = 11.84ALGVGAFVLSCAHH274 pKa = 6.4MGHH277 pKa = 6.51FLDD280 pKa = 5.84HH281 pKa = 6.12TFNWNIYY288 pKa = 9.89AIAFLLPNQQIGMWAGIIALGLMIPLALTSFDD320 pKa = 3.56FAVAKK325 pKa = 10.73LGTAWRR331 pKa = 11.84KK332 pKa = 8.23IHH334 pKa = 6.35LVSIPVLILAVIHH347 pKa = 6.17TSLTGSNYY355 pKa = 10.31LGEE358 pKa = 4.38INSDD362 pKa = 4.16FIHH365 pKa = 7.02RR366 pKa = 11.84FRR368 pKa = 11.84VGILIFLVITVLLIRR383 pKa = 11.84LQIFWSLFSLEE394 pKa = 4.14KK395 pKa = 10.31FYY397 pKa = 11.13AASKK401 pKa = 10.24RR402 pKa = 3.7

Molecular weight: 43.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6678

0

6678

2078917

29

7046

311.3

34.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.975 ± 0.033

0.962 ± 0.012

5.072 ± 0.025

6.149 ± 0.036

3.944 ± 0.021

6.561 ± 0.039

1.901 ± 0.016

6.373 ± 0.022

4.034 ± 0.03

10.881 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.972 ± 0.015

3.688 ± 0.029

4.814 ± 0.03

5.651 ± 0.033

5.846 ± 0.028

6.602 ± 0.033

5.751 ± 0.04

6.619 ± 0.027

1.458 ± 0.014

2.744 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski