Amino acid dipepetide frequency for Mycobacterium phage SkiPole

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.975AlaAla: 12.975 ± 1.232
0.777AlaCys: 0.777 ± 0.181
6.278AlaAsp: 6.278 ± 0.6
6.219AlaGlu: 6.219 ± 0.787
3.169AlaPhe: 3.169 ± 0.512
8.73AlaGly: 8.73 ± 0.911
1.674AlaHis: 1.674 ± 0.315
3.946AlaIle: 3.946 ± 0.485
4.186AlaLys: 4.186 ± 0.506
9.029AlaLeu: 9.029 ± 0.812
2.751AlaMet: 2.751 ± 0.308
2.153AlaAsn: 2.153 ± 0.324
5.023AlaPro: 5.023 ± 0.748
2.751AlaGln: 2.751 ± 0.446
6.458AlaArg: 6.458 ± 0.525
4.843AlaSer: 4.843 ± 0.46
5.68AlaThr: 5.68 ± 0.512
8.73AlaVal: 8.73 ± 0.768
1.913AlaTrp: 1.913 ± 0.353
2.332AlaTyr: 2.332 ± 0.413
0.0AlaXaa: 0.0 ± 0.0
Cys
1.076CysAla: 1.076 ± 0.321
0.06CysCys: 0.06 ± 0.057
0.658CysAsp: 0.658 ± 0.165
0.658CysGlu: 0.658 ± 0.195
0.179CysPhe: 0.179 ± 0.1
0.598CysGly: 0.598 ± 0.195
0.12CysHis: 0.12 ± 0.076
0.179CysIle: 0.179 ± 0.096
0.299CysLys: 0.299 ± 0.133
0.478CysLeu: 0.478 ± 0.17
0.12CysMet: 0.12 ± 0.092
0.299CysAsn: 0.299 ± 0.141
0.359CysPro: 0.359 ± 0.144
0.239CysGln: 0.239 ± 0.11
0.538CysArg: 0.538 ± 0.215
0.478CysSer: 0.478 ± 0.161
0.359CysThr: 0.359 ± 0.14
0.239CysVal: 0.239 ± 0.117
0.239CysTrp: 0.239 ± 0.111
0.12CysTyr: 0.12 ± 0.066
0.0CysXaa: 0.0 ± 0.0
Asp
6.458AspAla: 6.458 ± 0.53
0.419AspCys: 0.419 ± 0.138
4.843AspAsp: 4.843 ± 0.554
3.946AspGlu: 3.946 ± 0.557
2.212AspPhe: 2.212 ± 0.353
6.039AspGly: 6.039 ± 0.506
0.837AspHis: 0.837 ± 0.215
2.332AspIle: 2.332 ± 0.404
2.691AspLys: 2.691 ± 0.378
6.876AspLeu: 6.876 ± 0.662
1.256AspMet: 1.256 ± 0.222
1.913AspAsn: 1.913 ± 0.359
4.724AspPro: 4.724 ± 0.509
1.495AspGln: 1.495 ± 0.262
3.528AspArg: 3.528 ± 0.385
3.229AspSer: 3.229 ± 0.477
3.468AspThr: 3.468 ± 0.419
4.485AspVal: 4.485 ± 0.477
2.153AspTrp: 2.153 ± 0.366
2.033AspTyr: 2.033 ± 0.31
0.0AspXaa: 0.0 ± 0.0
Glu
5.86GluAla: 5.86 ± 0.618
0.359GluCys: 0.359 ± 0.171
4.963GluAsp: 4.963 ± 0.575
4.843GluGlu: 4.843 ± 0.559
2.093GluPhe: 2.093 ± 0.343
3.887GluGly: 3.887 ± 0.412
1.315GluHis: 1.315 ± 0.288
3.468GluIle: 3.468 ± 0.53
2.691GluLys: 2.691 ± 0.437
6.817GluLeu: 6.817 ± 0.615
1.555GluMet: 1.555 ± 0.262
1.794GluAsn: 1.794 ± 0.383
2.571GluPro: 2.571 ± 0.413
2.631GluGln: 2.631 ± 0.366
4.006GluArg: 4.006 ± 0.527
3.229GluSer: 3.229 ± 0.355
4.066GluThr: 4.066 ± 0.492
5.621GluVal: 5.621 ± 0.569
1.315GluTrp: 1.315 ± 0.331
2.392GluTyr: 2.392 ± 0.422
0.0GluXaa: 0.0 ± 0.0
Phe
2.511PheAla: 2.511 ± 0.416
0.299PheCys: 0.299 ± 0.16
2.99PheAsp: 2.99 ± 0.382
2.332PheGlu: 2.332 ± 0.336
0.478PhePhe: 0.478 ± 0.151
3.408PheGly: 3.408 ± 0.473
0.777PheHis: 0.777 ± 0.237
1.435PheIle: 1.435 ± 0.265
1.076PheLys: 1.076 ± 0.262
2.153PheLeu: 2.153 ± 0.359
0.538PheMet: 0.538 ± 0.16
1.674PheAsn: 1.674 ± 0.253
1.495PhePro: 1.495 ± 0.254
0.837PheGln: 0.837 ± 0.223
1.674PheArg: 1.674 ± 0.31
1.614PheSer: 1.614 ± 0.288
2.332PheThr: 2.332 ± 0.324
1.854PheVal: 1.854 ± 0.353
0.598PheTrp: 0.598 ± 0.175
0.837PheTyr: 0.837 ± 0.187
0.0PheXaa: 0.0 ± 0.0
Gly
7.414GlyAla: 7.414 ± 1.442
0.837GlyCys: 0.837 ± 0.263
5.501GlyAsp: 5.501 ± 0.498
4.485GlyGlu: 4.485 ± 0.468
2.751GlyPhe: 2.751 ± 0.467
10.165GlyGly: 10.165 ± 3.166
1.794GlyHis: 1.794 ± 0.312
4.006GlyIle: 4.006 ± 0.655
3.827GlyLys: 3.827 ± 0.48
7.474GlyLeu: 7.474 ± 0.632
2.033GlyMet: 2.033 ± 0.37
2.87GlyAsn: 2.87 ± 0.419
4.245GlyPro: 4.245 ± 0.628
2.511GlyGln: 2.511 ± 0.277
4.963GlyArg: 4.963 ± 0.616
6.338GlySer: 6.338 ± 0.782
5.083GlyThr: 5.083 ± 0.589
5.979GlyVal: 5.979 ± 0.721
2.691GlyTrp: 2.691 ± 0.339
2.751GlyTyr: 2.751 ± 0.363
0.0GlyXaa: 0.0 ± 0.0
His
1.614HisAla: 1.614 ± 0.328
0.299HisCys: 0.299 ± 0.149
1.256HisAsp: 1.256 ± 0.241
1.555HisGlu: 1.555 ± 0.329
0.598HisPhe: 0.598 ± 0.202
1.435HisGly: 1.435 ± 0.359
0.478HisHis: 0.478 ± 0.159
0.837HisIle: 0.837 ± 0.185
0.957HisLys: 0.957 ± 0.254
1.017HisLeu: 1.017 ± 0.285
0.239HisMet: 0.239 ± 0.127
0.419HisAsn: 0.419 ± 0.152
1.435HisPro: 1.435 ± 0.242
1.136HisGln: 1.136 ± 0.267
1.375HisArg: 1.375 ± 0.243
0.897HisSer: 0.897 ± 0.203
1.136HisThr: 1.136 ± 0.256
1.555HisVal: 1.555 ± 0.324
0.538HisTrp: 0.538 ± 0.162
0.538HisTyr: 0.538 ± 0.168
0.0HisXaa: 0.0 ± 0.0
Ile
6.458IleAla: 6.458 ± 0.669
0.299IleCys: 0.299 ± 0.136
3.408IleAsp: 3.408 ± 0.369
3.946IleGlu: 3.946 ± 0.453
1.136IlePhe: 1.136 ± 0.27
3.887IleGly: 3.887 ± 0.451
0.837IleHis: 0.837 ± 0.241
1.794IleIle: 1.794 ± 0.278
1.794IleLys: 1.794 ± 0.331
3.05IleLeu: 3.05 ± 0.395
0.837IleMet: 0.837 ± 0.213
1.794IleAsn: 1.794 ± 0.321
2.87IlePro: 2.87 ± 0.355
1.196IleGln: 1.196 ± 0.292
3.289IleArg: 3.289 ± 0.44
3.109IleSer: 3.109 ± 0.395
3.109IleThr: 3.109 ± 0.4
3.109IleVal: 3.109 ± 0.505
0.777IleTrp: 0.777 ± 0.202
1.435IleTyr: 1.435 ± 0.264
0.0IleXaa: 0.0 ± 0.0
Lys
3.348LysAla: 3.348 ± 0.533
0.299LysCys: 0.299 ± 0.135
2.033LysAsp: 2.033 ± 0.373
2.093LysGlu: 2.093 ± 0.36
1.614LysPhe: 1.614 ± 0.253
2.571LysGly: 2.571 ± 0.394
1.196LysHis: 1.196 ± 0.322
2.392LysIle: 2.392 ± 0.386
2.272LysLys: 2.272 ± 0.331
3.827LysLeu: 3.827 ± 0.45
1.076LysMet: 1.076 ± 0.215
1.435LysAsn: 1.435 ± 0.311
2.511LysPro: 2.511 ± 0.404
1.614LysGln: 1.614 ± 0.322
2.87LysArg: 2.87 ± 0.44
2.511LysSer: 2.511 ± 0.342
2.631LysThr: 2.631 ± 0.429
3.408LysVal: 3.408 ± 0.466
0.718LysTrp: 0.718 ± 0.19
0.837LysTyr: 0.837 ± 0.197
0.0LysXaa: 0.0 ± 0.0
Leu
8.73LeuAla: 8.73 ± 0.722
0.478LeuCys: 0.478 ± 0.159
6.219LeuAsp: 6.219 ± 0.617
5.262LeuGlu: 5.262 ± 0.541
2.093LeuPhe: 2.093 ± 0.344
7.355LeuGly: 7.355 ± 0.666
1.674LeuHis: 1.674 ± 0.301
4.664LeuIle: 4.664 ± 0.482
4.126LeuLys: 4.126 ± 0.546
5.561LeuLeu: 5.561 ± 0.468
1.674LeuMet: 1.674 ± 0.334
2.631LeuAsn: 2.631 ± 0.376
5.8LeuPro: 5.8 ± 0.519
2.751LeuGln: 2.751 ± 0.6
6.338LeuArg: 6.338 ± 0.693
6.159LeuSer: 6.159 ± 0.585
5.74LeuThr: 5.74 ± 0.559
4.604LeuVal: 4.604 ± 0.688
0.957LeuTrp: 0.957 ± 0.283
1.973LeuTyr: 1.973 ± 0.382
0.0LeuXaa: 0.0 ± 0.0
Met
2.691MetAla: 2.691 ± 0.349
0.0MetCys: 0.0 ± 0.0
1.196MetAsp: 1.196 ± 0.223
1.614MetGlu: 1.614 ± 0.316
0.538MetPhe: 0.538 ± 0.173
1.315MetGly: 1.315 ± 0.259
0.179MetHis: 0.179 ± 0.105
0.837MetIle: 0.837 ± 0.239
0.957MetLys: 0.957 ± 0.229
1.256MetLeu: 1.256 ± 0.246
0.12MetMet: 0.12 ± 0.095
1.017MetAsn: 1.017 ± 0.214
1.076MetPro: 1.076 ± 0.296
0.658MetGln: 0.658 ± 0.192
1.734MetArg: 1.734 ± 0.304
2.212MetSer: 2.212 ± 0.376
2.392MetThr: 2.392 ± 0.312
1.017MetVal: 1.017 ± 0.224
0.359MetTrp: 0.359 ± 0.125
0.359MetTyr: 0.359 ± 0.17
0.0MetXaa: 0.0 ± 0.0
Asn
3.169AsnAla: 3.169 ± 0.475
0.12AsnCys: 0.12 ± 0.084
1.734AsnAsp: 1.734 ± 0.395
1.913AsnGlu: 1.913 ± 0.389
0.957AsnPhe: 0.957 ± 0.206
3.408AsnGly: 3.408 ± 0.42
0.598AsnHis: 0.598 ± 0.154
1.435AsnIle: 1.435 ± 0.274
0.718AsnLys: 0.718 ± 0.193
2.631AsnLeu: 2.631 ± 0.325
0.658AsnMet: 0.658 ± 0.175
0.897AsnAsn: 0.897 ± 0.208
2.87AsnPro: 2.87 ± 0.335
0.837AsnGln: 0.837 ± 0.222
1.315AsnArg: 1.315 ± 0.31
1.854AsnSer: 1.854 ± 0.353
1.913AsnThr: 1.913 ± 0.35
2.452AsnVal: 2.452 ± 0.368
0.777AsnTrp: 0.777 ± 0.199
1.256AsnTyr: 1.256 ± 0.277
0.0AsnXaa: 0.0 ± 0.0
Pro
5.441ProAla: 5.441 ± 0.625
0.478ProCys: 0.478 ± 0.202
3.887ProAsp: 3.887 ± 0.451
4.485ProGlu: 4.485 ± 0.503
2.153ProPhe: 2.153 ± 0.316
4.843ProGly: 4.843 ± 0.588
0.897ProHis: 0.897 ± 0.203
2.272ProIle: 2.272 ± 0.359
2.392ProLys: 2.392 ± 0.336
4.365ProLeu: 4.365 ± 0.509
1.196ProMet: 1.196 ± 0.3
1.435ProAsn: 1.435 ± 0.277
2.571ProPro: 2.571 ± 0.402
1.495ProGln: 1.495 ± 0.368
3.05ProArg: 3.05 ± 0.534
3.827ProSer: 3.827 ± 0.443
3.946ProThr: 3.946 ± 0.531
4.006ProVal: 4.006 ± 0.47
1.017ProTrp: 1.017 ± 0.309
1.495ProTyr: 1.495 ± 0.312
0.0ProXaa: 0.0 ± 0.0
Gln
2.81GlnAla: 2.81 ± 0.441
0.12GlnCys: 0.12 ± 0.081
1.256GlnAsp: 1.256 ± 0.283
1.614GlnGlu: 1.614 ± 0.273
0.897GlnPhe: 0.897 ± 0.193
2.452GlnGly: 2.452 ± 0.312
0.658GlnHis: 0.658 ± 0.192
2.81GlnIle: 2.81 ± 0.469
1.076GlnLys: 1.076 ± 0.326
3.528GlnLeu: 3.528 ± 0.493
1.136GlnMet: 1.136 ± 0.247
0.359GlnAsn: 0.359 ± 0.125
1.973GlnPro: 1.973 ± 0.37
1.734GlnGln: 1.734 ± 0.326
1.913GlnArg: 1.913 ± 0.361
1.854GlnSer: 1.854 ± 0.281
1.973GlnThr: 1.973 ± 0.334
2.571GlnVal: 2.571 ± 0.329
0.538GlnTrp: 0.538 ± 0.158
0.478GlnTyr: 0.478 ± 0.149
0.0GlnXaa: 0.0 ± 0.0
Arg
6.099ArgAla: 6.099 ± 0.714
0.658ArgCys: 0.658 ± 0.188
3.348ArgAsp: 3.348 ± 0.43
4.485ArgGlu: 4.485 ± 0.6
1.854ArgPhe: 1.854 ± 0.318
5.262ArgGly: 5.262 ± 0.83
1.136ArgHis: 1.136 ± 0.273
3.647ArgIle: 3.647 ± 0.509
2.87ArgLys: 2.87 ± 0.445
5.68ArgLeu: 5.68 ± 0.622
1.913ArgMet: 1.913 ± 0.323
2.452ArgAsn: 2.452 ± 0.469
2.272ArgPro: 2.272 ± 0.354
1.973ArgGln: 1.973 ± 0.279
6.039ArgArg: 6.039 ± 0.694
3.528ArgSer: 3.528 ± 0.447
3.109ArgThr: 3.109 ± 0.41
5.381ArgVal: 5.381 ± 0.61
1.315ArgTrp: 1.315 ± 0.294
1.913ArgTyr: 1.913 ± 0.27
0.0ArgXaa: 0.0 ± 0.0
Ser
6.338SerAla: 6.338 ± 0.664
0.538SerCys: 0.538 ± 0.183
3.408SerAsp: 3.408 ± 0.358
4.006SerGlu: 4.006 ± 0.489
2.212SerPhe: 2.212 ± 0.342
6.458SerGly: 6.458 ± 0.79
1.375SerHis: 1.375 ± 0.257
2.691SerIle: 2.691 ± 0.407
1.913SerLys: 1.913 ± 0.311
4.664SerLeu: 4.664 ± 0.55
1.435SerMet: 1.435 ± 0.241
2.272SerAsn: 2.272 ± 0.338
3.05SerPro: 3.05 ± 0.451
1.973SerGln: 1.973 ± 0.25
3.289SerArg: 3.289 ± 0.45
3.887SerSer: 3.887 ± 0.679
3.588SerThr: 3.588 ± 0.482
4.066SerVal: 4.066 ± 0.53
1.375SerTrp: 1.375 ± 0.26
1.734SerTyr: 1.734 ± 0.309
0.0SerXaa: 0.0 ± 0.0
Thr
5.621ThrAla: 5.621 ± 0.605
0.359ThrCys: 0.359 ± 0.149
4.126ThrAsp: 4.126 ± 0.545
4.066ThrGlu: 4.066 ± 0.502
2.212ThrPhe: 2.212 ± 0.412
6.637ThrGly: 6.637 ± 0.632
1.256ThrHis: 1.256 ± 0.347
2.87ThrIle: 2.87 ± 0.51
2.99ThrLys: 2.99 ± 0.396
6.278ThrLeu: 6.278 ± 0.598
1.017ThrMet: 1.017 ± 0.19
1.674ThrAsn: 1.674 ± 0.341
4.126ThrPro: 4.126 ± 0.574
1.734ThrGln: 1.734 ± 0.348
3.408ThrArg: 3.408 ± 0.502
3.289ThrSer: 3.289 ± 0.574
3.827ThrThr: 3.827 ± 0.654
5.441ThrVal: 5.441 ± 0.573
0.837ThrTrp: 0.837 ± 0.227
2.033ThrTyr: 2.033 ± 0.319
0.0ThrXaa: 0.0 ± 0.0
Val
7.235ValAla: 7.235 ± 0.704
0.419ValCys: 0.419 ± 0.146
5.381ValAsp: 5.381 ± 0.6
4.784ValGlu: 4.784 ± 0.553
2.272ValPhe: 2.272 ± 0.295
4.903ValGly: 4.903 ± 0.684
1.315ValHis: 1.315 ± 0.237
3.946ValIle: 3.946 ± 0.419
2.99ValLys: 2.99 ± 0.362
5.8ValLeu: 5.8 ± 0.576
0.897ValMet: 0.897 ± 0.231
2.571ValAsn: 2.571 ± 0.327
4.305ValPro: 4.305 ± 0.559
2.272ValGln: 2.272 ± 0.384
5.142ValArg: 5.142 ± 0.557
4.425ValSer: 4.425 ± 0.452
5.86ValThr: 5.86 ± 0.693
5.441ValVal: 5.441 ± 0.697
1.375ValTrp: 1.375 ± 0.289
2.272ValTyr: 2.272 ± 0.321
0.0ValXaa: 0.0 ± 0.0
Trp
1.435TrpAla: 1.435 ± 0.279
0.239TrpCys: 0.239 ± 0.119
1.495TrpAsp: 1.495 ± 0.272
1.076TrpGlu: 1.076 ± 0.223
0.897TrpPhe: 0.897 ± 0.23
1.794TrpGly: 1.794 ± 0.286
0.538TrpHis: 0.538 ± 0.18
0.957TrpIle: 0.957 ± 0.225
0.299TrpLys: 0.299 ± 0.136
1.973TrpLeu: 1.973 ± 0.311
0.478TrpMet: 0.478 ± 0.164
0.538TrpAsn: 0.538 ± 0.19
0.718TrpPro: 0.718 ± 0.213
0.957TrpGln: 0.957 ± 0.22
1.375TrpArg: 1.375 ± 0.324
1.315TrpSer: 1.315 ± 0.244
1.435TrpThr: 1.435 ± 0.323
1.973TrpVal: 1.973 ± 0.356
0.478TrpTrp: 0.478 ± 0.196
0.299TrpTyr: 0.299 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.332TyrAla: 2.332 ± 0.365
0.239TyrCys: 0.239 ± 0.145
0.957TyrAsp: 0.957 ± 0.289
2.093TyrGlu: 2.093 ± 0.35
0.658TyrPhe: 0.658 ± 0.19
2.571TyrGly: 2.571 ± 0.431
0.658TyrHis: 0.658 ± 0.199
1.614TyrIle: 1.614 ± 0.297
1.136TyrLys: 1.136 ± 0.276
2.332TyrLeu: 2.332 ± 0.381
0.538TyrMet: 0.538 ± 0.181
1.315TyrAsn: 1.315 ± 0.325
1.256TyrPro: 1.256 ± 0.285
0.957TyrGln: 0.957 ± 0.271
2.631TyrArg: 2.631 ± 0.346
1.674TyrSer: 1.674 ± 0.304
2.153TyrThr: 2.153 ± 0.35
1.674TyrVal: 1.674 ± 0.308
0.299TyrTrp: 0.299 ± 0.129
0.538TyrTyr: 0.538 ± 0.182
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 102 proteins (16725 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski