Amino acid dipepetide frequency for Lactobacillus phage phiPYB5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.275AlaAla: 4.275 ± 0.717
0.597AlaCys: 0.597 ± 0.232
5.866AlaAsp: 5.866 ± 0.667
3.48AlaGlu: 3.48 ± 0.757
2.486AlaPhe: 2.486 ± 0.43
4.673AlaGly: 4.673 ± 0.568
1.193AlaHis: 1.193 ± 0.314
6.463AlaIle: 6.463 ± 0.734
6.86AlaLys: 6.86 ± 0.902
6.661AlaLeu: 6.661 ± 0.888
1.889AlaMet: 1.889 ± 0.475
6.164AlaAsn: 6.164 ± 0.736
1.193AlaPro: 1.193 ± 0.333
4.176AlaGln: 4.176 ± 0.658
3.182AlaArg: 3.182 ± 0.467
4.673AlaSer: 4.673 ± 0.869
4.872AlaThr: 4.872 ± 0.609
4.573AlaVal: 4.573 ± 0.759
1.293AlaTrp: 1.293 ± 0.333
2.486AlaTyr: 2.486 ± 0.4
0.0AlaXaa: 0.0 ± 0.0
Cys
0.298CysAla: 0.298 ± 0.167
0.0CysCys: 0.0 ± 0.0
0.099CysAsp: 0.099 ± 0.093
0.597CysGlu: 0.597 ± 0.245
0.199CysPhe: 0.199 ± 0.179
0.597CysGly: 0.597 ± 0.29
0.099CysHis: 0.099 ± 0.092
0.199CysIle: 0.199 ± 0.176
0.298CysLys: 0.298 ± 0.157
0.497CysLeu: 0.497 ± 0.181
0.199CysMet: 0.199 ± 0.117
0.099CysAsn: 0.099 ± 0.09
0.298CysPro: 0.298 ± 0.154
0.0CysGln: 0.0 ± 0.0
0.398CysArg: 0.398 ± 0.177
0.696CysSer: 0.696 ± 0.27
0.298CysThr: 0.298 ± 0.151
0.398CysVal: 0.398 ± 0.23
0.199CysTrp: 0.199 ± 0.124
0.099CysTyr: 0.099 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
4.971AspAla: 4.971 ± 0.624
0.696AspCys: 0.696 ± 0.28
6.065AspAsp: 6.065 ± 1.031
3.082AspGlu: 3.082 ± 0.565
2.883AspPhe: 2.883 ± 0.593
5.468AspGly: 5.468 ± 0.633
0.795AspHis: 0.795 ± 0.279
3.679AspIle: 3.679 ± 0.535
4.573AspLys: 4.573 ± 0.834
5.965AspLeu: 5.965 ± 0.796
1.79AspMet: 1.79 ± 0.406
3.579AspAsn: 3.579 ± 0.667
2.486AspPro: 2.486 ± 0.456
2.684AspGln: 2.684 ± 0.473
3.082AspArg: 3.082 ± 0.505
5.071AspSer: 5.071 ± 0.795
4.772AspThr: 4.772 ± 0.718
4.275AspVal: 4.275 ± 0.531
1.591AspTrp: 1.591 ± 0.3
4.375AspTyr: 4.375 ± 0.651
0.0AspXaa: 0.0 ± 0.0
Glu
3.878GluAla: 3.878 ± 0.769
0.099GluCys: 0.099 ± 0.111
3.182GluAsp: 3.182 ± 0.504
3.48GluGlu: 3.48 ± 0.689
1.69GluPhe: 1.69 ± 0.48
2.784GluGly: 2.784 ± 0.45
0.994GluHis: 0.994 ± 0.306
3.281GluIle: 3.281 ± 0.615
4.474GluLys: 4.474 ± 0.873
4.673GluLeu: 4.673 ± 0.626
1.392GluMet: 1.392 ± 0.354
2.287GluAsn: 2.287 ± 0.633
2.088GluPro: 2.088 ± 0.535
1.69GluGln: 1.69 ± 0.484
2.287GluArg: 2.287 ± 0.629
3.38GluSer: 3.38 ± 0.48
2.187GluThr: 2.187 ± 0.479
4.076GluVal: 4.076 ± 0.548
0.795GluTrp: 0.795 ± 0.271
2.287GluTyr: 2.287 ± 0.49
0.0GluXaa: 0.0 ± 0.0
Phe
2.187PheAla: 2.187 ± 0.421
0.298PheCys: 0.298 ± 0.155
2.585PheAsp: 2.585 ± 0.528
1.889PheGlu: 1.889 ± 0.442
1.293PhePhe: 1.293 ± 0.333
2.983PheGly: 2.983 ± 0.49
0.597PheHis: 0.597 ± 0.317
1.79PheIle: 1.79 ± 0.371
2.784PheLys: 2.784 ± 0.613
2.088PheLeu: 2.088 ± 0.65
0.994PheMet: 0.994 ± 0.272
2.486PheAsn: 2.486 ± 0.519
1.094PhePro: 1.094 ± 0.304
1.193PheGln: 1.193 ± 0.288
1.193PheArg: 1.193 ± 0.312
1.79PheSer: 1.79 ± 0.45
3.778PheThr: 3.778 ± 0.651
2.287PheVal: 2.287 ± 0.512
0.895PheTrp: 0.895 ± 0.287
1.193PheTyr: 1.193 ± 0.522
0.0PheXaa: 0.0 ± 0.0
Gly
4.772GlyAla: 4.772 ± 0.793
0.398GlyCys: 0.398 ± 0.186
6.264GlyAsp: 6.264 ± 0.828
3.281GlyGlu: 3.281 ± 0.589
3.679GlyPhe: 3.679 ± 0.73
4.673GlyGly: 4.673 ± 0.931
1.193GlyHis: 1.193 ± 0.359
4.275GlyIle: 4.275 ± 0.816
4.872GlyLys: 4.872 ± 0.683
5.568GlyLeu: 5.568 ± 0.702
1.79GlyMet: 1.79 ± 0.383
4.772GlyAsn: 4.772 ± 0.779
2.386GlyPro: 2.386 ± 0.973
2.983GlyGln: 2.983 ± 0.509
2.187GlyArg: 2.187 ± 0.507
4.176GlySer: 4.176 ± 0.701
5.468GlyThr: 5.468 ± 0.8
4.971GlyVal: 4.971 ± 0.868
1.79GlyTrp: 1.79 ± 0.451
3.38GlyTyr: 3.38 ± 0.708
0.0GlyXaa: 0.0 ± 0.0
His
0.994HisAla: 0.994 ± 0.268
0.0HisCys: 0.0 ± 0.0
1.094HisAsp: 1.094 ± 0.323
0.696HisGlu: 0.696 ± 0.362
1.094HisPhe: 1.094 ± 0.316
1.69HisGly: 1.69 ± 0.431
0.398HisHis: 0.398 ± 0.218
1.591HisIle: 1.591 ± 0.394
0.895HisLys: 0.895 ± 0.351
1.293HisLeu: 1.293 ± 0.339
0.597HisMet: 0.597 ± 0.249
0.795HisAsn: 0.795 ± 0.255
0.795HisPro: 0.795 ± 0.382
0.795HisGln: 0.795 ± 0.215
0.795HisArg: 0.795 ± 0.33
0.696HisSer: 0.696 ± 0.198
1.094HisThr: 1.094 ± 0.39
0.895HisVal: 0.895 ± 0.261
0.398HisTrp: 0.398 ± 0.206
0.795HisTyr: 0.795 ± 0.304
0.0HisXaa: 0.0 ± 0.0
Ile
5.667IleAla: 5.667 ± 0.709
0.497IleCys: 0.497 ± 0.173
3.679IleAsp: 3.679 ± 0.757
4.375IleGlu: 4.375 ± 0.59
1.79IlePhe: 1.79 ± 0.422
4.673IleGly: 4.673 ± 0.675
0.795IleHis: 0.795 ± 0.27
3.182IleIle: 3.182 ± 0.618
6.264IleLys: 6.264 ± 0.916
4.176IleLeu: 4.176 ± 0.618
1.889IleMet: 1.889 ± 0.444
3.878IleAsn: 3.878 ± 0.674
2.784IlePro: 2.784 ± 0.473
1.988IleGln: 1.988 ± 0.42
2.486IleArg: 2.486 ± 0.457
4.971IleSer: 4.971 ± 0.625
4.872IleThr: 4.872 ± 0.692
4.076IleVal: 4.076 ± 0.577
0.895IleTrp: 0.895 ± 0.453
2.486IleTyr: 2.486 ± 0.53
0.0IleXaa: 0.0 ± 0.0
Lys
7.457LysAla: 7.457 ± 0.975
0.298LysCys: 0.298 ± 0.151
3.679LysAsp: 3.679 ± 0.675
3.579LysGlu: 3.579 ± 0.628
1.79LysPhe: 1.79 ± 0.326
5.468LysGly: 5.468 ± 0.794
1.094LysHis: 1.094 ± 0.34
4.872LysIle: 4.872 ± 0.701
3.679LysLys: 3.679 ± 0.71
6.96LysLeu: 6.96 ± 0.996
2.287LysMet: 2.287 ± 0.467
3.579LysAsn: 3.579 ± 0.538
1.988LysPro: 1.988 ± 0.557
4.076LysGln: 4.076 ± 0.913
3.679LysArg: 3.679 ± 0.81
4.872LysSer: 4.872 ± 0.676
4.872LysThr: 4.872 ± 0.773
3.281LysVal: 3.281 ± 0.508
1.193LysTrp: 1.193 ± 0.308
1.889LysTyr: 1.889 ± 0.372
0.0LysXaa: 0.0 ± 0.0
Leu
5.767LeuAla: 5.767 ± 0.68
0.0LeuCys: 0.0 ± 0.0
7.556LeuAsp: 7.556 ± 1.063
4.076LeuGlu: 4.076 ± 0.601
1.988LeuPhe: 1.988 ± 0.394
4.474LeuGly: 4.474 ± 0.641
1.591LeuHis: 1.591 ± 0.315
4.673LeuIle: 4.673 ± 0.835
6.463LeuLys: 6.463 ± 0.778
4.772LeuLeu: 4.772 ± 0.769
1.491LeuMet: 1.491 ± 0.305
4.275LeuAsn: 4.275 ± 0.737
2.486LeuPro: 2.486 ± 0.409
3.082LeuGln: 3.082 ± 0.548
3.182LeuArg: 3.182 ± 0.496
7.755LeuSer: 7.755 ± 0.869
5.866LeuThr: 5.866 ± 0.715
5.667LeuVal: 5.667 ± 0.686
1.293LeuTrp: 1.293 ± 0.35
2.187LeuTyr: 2.187 ± 0.508
0.0LeuXaa: 0.0 ± 0.0
Met
2.784MetAla: 2.784 ± 0.5
0.0MetCys: 0.0 ± 0.0
1.889MetAsp: 1.889 ± 0.468
1.69MetGlu: 1.69 ± 0.518
0.895MetPhe: 0.895 ± 0.251
1.293MetGly: 1.293 ± 0.356
0.398MetHis: 0.398 ± 0.21
2.088MetIle: 2.088 ± 0.493
1.79MetLys: 1.79 ± 0.371
1.69MetLeu: 1.69 ± 0.492
0.696MetMet: 0.696 ± 0.214
1.293MetAsn: 1.293 ± 0.363
1.293MetPro: 1.293 ± 0.377
1.392MetGln: 1.392 ± 0.338
0.795MetArg: 0.795 ± 0.264
1.293MetSer: 1.293 ± 0.302
1.988MetThr: 1.988 ± 0.516
0.994MetVal: 0.994 ± 0.286
0.398MetTrp: 0.398 ± 0.213
0.696MetTyr: 0.696 ± 0.235
0.0MetXaa: 0.0 ± 0.0
Asn
4.275AsnAla: 4.275 ± 0.575
0.497AsnCys: 0.497 ± 0.201
3.281AsnAsp: 3.281 ± 0.503
4.474AsnGlu: 4.474 ± 0.794
1.889AsnPhe: 1.889 ± 0.404
5.965AsnGly: 5.965 ± 0.88
1.094AsnHis: 1.094 ± 0.288
3.182AsnIle: 3.182 ± 0.523
3.38AsnLys: 3.38 ± 0.493
3.579AsnLeu: 3.579 ± 0.697
1.392AsnMet: 1.392 ± 0.336
3.878AsnAsn: 3.878 ± 0.671
2.684AsnPro: 2.684 ± 0.683
2.486AsnGln: 2.486 ± 0.548
2.486AsnArg: 2.486 ± 0.557
3.182AsnSer: 3.182 ± 0.621
2.784AsnThr: 2.784 ± 0.552
3.977AsnVal: 3.977 ± 0.736
0.696AsnTrp: 0.696 ± 0.225
2.486AsnTyr: 2.486 ± 0.487
0.0AsnXaa: 0.0 ± 0.0
Pro
3.082ProAla: 3.082 ± 0.503
0.0ProCys: 0.0 ± 0.0
3.082ProAsp: 3.082 ± 0.533
2.088ProGlu: 2.088 ± 0.573
0.895ProPhe: 0.895 ± 0.349
1.591ProGly: 1.591 ± 0.432
0.895ProHis: 0.895 ± 0.267
1.988ProIle: 1.988 ± 0.466
2.585ProLys: 2.585 ± 0.487
2.585ProLeu: 2.585 ± 0.517
0.696ProMet: 0.696 ± 0.232
1.889ProAsn: 1.889 ± 0.454
0.795ProPro: 0.795 ± 0.248
1.69ProGln: 1.69 ± 0.545
1.094ProArg: 1.094 ± 0.336
2.287ProSer: 2.287 ± 0.671
3.48ProThr: 3.48 ± 0.597
2.684ProVal: 2.684 ± 0.443
0.298ProTrp: 0.298 ± 0.147
1.491ProTyr: 1.491 ± 0.302
0.0ProXaa: 0.0 ± 0.0
Gln
4.772GlnAla: 4.772 ± 0.775
0.298GlnCys: 0.298 ± 0.164
2.585GlnAsp: 2.585 ± 0.472
1.889GlnGlu: 1.889 ± 0.4
1.392GlnPhe: 1.392 ± 0.382
2.287GlnGly: 2.287 ± 0.777
0.597GlnHis: 0.597 ± 0.304
3.679GlnIle: 3.679 ± 0.775
2.585GlnLys: 2.585 ± 0.411
3.878GlnLeu: 3.878 ± 0.629
1.69GlnMet: 1.69 ± 0.387
2.287GlnAsn: 2.287 ± 0.54
1.293GlnPro: 1.293 ± 0.307
1.79GlnGln: 1.79 ± 0.324
1.591GlnArg: 1.591 ± 0.331
2.585GlnSer: 2.585 ± 0.561
2.486GlnThr: 2.486 ± 0.547
2.784GlnVal: 2.784 ± 0.659
0.298GlnTrp: 0.298 ± 0.19
1.79GlnTyr: 1.79 ± 0.547
0.0GlnXaa: 0.0 ± 0.0
Arg
2.386ArgAla: 2.386 ± 0.52
0.199ArgCys: 0.199 ± 0.167
3.48ArgAsp: 3.48 ± 0.622
1.79ArgGlu: 1.79 ± 0.401
1.988ArgPhe: 1.988 ± 0.535
2.585ArgGly: 2.585 ± 0.443
1.193ArgHis: 1.193 ± 0.38
2.187ArgIle: 2.187 ± 0.538
2.187ArgLys: 2.187 ± 0.412
3.977ArgLeu: 3.977 ± 0.589
0.398ArgMet: 0.398 ± 0.187
1.392ArgAsn: 1.392 ± 0.283
1.591ArgPro: 1.591 ± 0.464
2.684ArgGln: 2.684 ± 0.6
2.386ArgArg: 2.386 ± 0.604
2.784ArgSer: 2.784 ± 0.427
1.491ArgThr: 1.491 ± 0.295
2.287ArgVal: 2.287 ± 0.519
0.994ArgTrp: 0.994 ± 0.264
2.187ArgTyr: 2.187 ± 0.625
0.0ArgXaa: 0.0 ± 0.0
Ser
3.878SerAla: 3.878 ± 0.571
0.099SerCys: 0.099 ± 0.088
4.474SerAsp: 4.474 ± 0.686
2.187SerGlu: 2.187 ± 0.432
2.983SerPhe: 2.983 ± 0.658
6.264SerGly: 6.264 ± 0.882
1.392SerHis: 1.392 ± 0.403
4.573SerIle: 4.573 ± 0.621
3.878SerLys: 3.878 ± 0.706
5.17SerLeu: 5.17 ± 0.75
1.293SerMet: 1.293 ± 0.371
4.971SerAsn: 4.971 ± 0.678
2.386SerPro: 2.386 ± 0.586
2.784SerGln: 2.784 ± 0.436
2.386SerArg: 2.386 ± 0.477
3.977SerSer: 3.977 ± 0.683
5.269SerThr: 5.269 ± 0.905
4.076SerVal: 4.076 ± 0.775
0.994SerTrp: 0.994 ± 0.284
2.983SerTyr: 2.983 ± 0.468
0.0SerXaa: 0.0 ± 0.0
Thr
5.17ThrAla: 5.17 ± 0.689
0.298ThrCys: 0.298 ± 0.165
3.679ThrAsp: 3.679 ± 0.644
2.883ThrGlu: 2.883 ± 0.546
2.486ThrPhe: 2.486 ± 0.427
6.264ThrGly: 6.264 ± 0.809
0.994ThrHis: 0.994 ± 0.3
5.866ThrIle: 5.866 ± 0.966
4.673ThrLys: 4.673 ± 0.702
5.866ThrLeu: 5.866 ± 0.836
1.69ThrMet: 1.69 ± 0.421
3.281ThrAsn: 3.281 ± 0.549
3.48ThrPro: 3.48 ± 0.578
2.088ThrGln: 2.088 ± 0.475
1.69ThrArg: 1.69 ± 0.37
4.474ThrSer: 4.474 ± 0.609
5.369ThrThr: 5.369 ± 0.976
4.176ThrVal: 4.176 ± 0.753
1.094ThrTrp: 1.094 ± 0.32
2.784ThrTyr: 2.784 ± 0.49
0.0ThrXaa: 0.0 ± 0.0
Val
6.164ValAla: 6.164 ± 0.882
0.795ValCys: 0.795 ± 0.218
4.772ValAsp: 4.772 ± 0.605
3.182ValGlu: 3.182 ± 0.55
1.889ValPhe: 1.889 ± 0.431
4.673ValGly: 4.673 ± 0.761
0.795ValHis: 0.795 ± 0.241
4.971ValIle: 4.971 ± 0.928
4.573ValLys: 4.573 ± 0.686
4.673ValLeu: 4.673 ± 0.638
1.392ValMet: 1.392 ± 0.41
3.679ValAsn: 3.679 ± 0.416
1.79ValPro: 1.79 ± 0.464
1.79ValGln: 1.79 ± 0.459
2.983ValArg: 2.983 ± 0.508
3.878ValSer: 3.878 ± 0.719
3.878ValThr: 3.878 ± 0.54
3.878ValVal: 3.878 ± 0.656
0.895ValTrp: 0.895 ± 0.342
1.988ValTyr: 1.988 ± 0.449
0.0ValXaa: 0.0 ± 0.0
Trp
1.293TrpAla: 1.293 ± 0.429
0.0TrpCys: 0.0 ± 0.0
1.094TrpAsp: 1.094 ± 0.294
0.696TrpGlu: 0.696 ± 0.212
0.696TrpPhe: 0.696 ± 0.256
1.094TrpGly: 1.094 ± 0.269
0.398TrpHis: 0.398 ± 0.206
1.094TrpIle: 1.094 ± 0.307
1.392TrpLys: 1.392 ± 0.515
1.094TrpLeu: 1.094 ± 0.32
0.497TrpMet: 0.497 ± 0.285
0.597TrpAsn: 0.597 ± 0.267
0.497TrpPro: 0.497 ± 0.212
1.392TrpGln: 1.392 ± 0.395
0.696TrpArg: 0.696 ± 0.257
1.491TrpSer: 1.491 ± 0.352
1.392TrpThr: 1.392 ± 0.35
0.696TrpVal: 0.696 ± 0.232
0.099TrpTrp: 0.099 ± 0.089
0.398TrpTyr: 0.398 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.983TyrAla: 2.983 ± 0.594
0.497TyrCys: 0.497 ± 0.25
3.48TyrAsp: 3.48 ± 0.707
1.591TyrGlu: 1.591 ± 0.355
1.392TyrPhe: 1.392 ± 0.307
3.38TyrGly: 3.38 ± 0.815
0.895TyrHis: 0.895 ± 0.313
1.79TyrIle: 1.79 ± 0.416
2.386TyrLys: 2.386 ± 0.426
3.48TyrLeu: 3.48 ± 0.537
1.193TyrMet: 1.193 ± 0.316
2.486TyrAsn: 2.486 ± 0.494
1.79TyrPro: 1.79 ± 0.383
1.889TyrGln: 1.889 ± 0.365
1.591TyrArg: 1.591 ± 0.38
1.988TyrSer: 1.988 ± 0.355
2.088TyrThr: 2.088 ± 0.472
2.585TyrVal: 2.585 ± 0.385
0.497TyrTrp: 0.497 ± 0.192
1.69TyrTyr: 1.69 ± 0.404
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (10059 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski