Amino acid dipepetide frequency for Proteiniclasticum ruminis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.074AlaAla: 5.074 ± 0.097
0.527AlaCys: 0.527 ± 0.024
2.912AlaAsp: 2.912 ± 0.066
4.497AlaGlu: 4.497 ± 0.074
3.211AlaPhe: 3.211 ± 0.056
4.647AlaGly: 4.647 ± 0.091
1.075AlaHis: 1.075 ± 0.037
4.556AlaIle: 4.556 ± 0.077
4.528AlaLys: 4.528 ± 0.084
7.864AlaLeu: 7.864 ± 0.116
2.304AlaMet: 2.304 ± 0.055
2.006AlaAsn: 2.006 ± 0.047
1.854AlaPro: 1.854 ± 0.058
1.701AlaGln: 1.701 ± 0.037
2.543AlaArg: 2.543 ± 0.054
4.055AlaSer: 4.055 ± 0.07
2.875AlaThr: 2.875 ± 0.058
5.483AlaVal: 5.483 ± 0.101
0.386AlaTrp: 0.386 ± 0.022
2.627AlaTyr: 2.627 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.453CysAla: 0.453 ± 0.021
0.087CysCys: 0.087 ± 0.01
0.435CysAsp: 0.435 ± 0.024
0.473CysGlu: 0.473 ± 0.022
0.261CysPhe: 0.261 ± 0.017
0.713CysGly: 0.713 ± 0.033
0.223CysHis: 0.223 ± 0.016
0.514CysIle: 0.514 ± 0.028
0.413CysLys: 0.413 ± 0.024
0.515CysLeu: 0.515 ± 0.022
0.163CysMet: 0.163 ± 0.013
0.276CysAsn: 0.276 ± 0.018
0.347CysPro: 0.347 ± 0.019
0.229CysGln: 0.229 ± 0.016
0.264CysArg: 0.264 ± 0.016
0.478CysSer: 0.478 ± 0.023
0.374CysThr: 0.374 ± 0.02
0.408CysVal: 0.408 ± 0.022
0.041CysTrp: 0.041 ± 0.007
0.276CysTyr: 0.276 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
3.692AspAla: 3.692 ± 0.064
0.344AspCys: 0.344 ± 0.021
2.141AspAsp: 2.141 ± 0.055
4.505AspGlu: 4.505 ± 0.078
2.703AspPhe: 2.703 ± 0.058
3.685AspGly: 3.685 ± 0.069
1.151AspHis: 1.151 ± 0.041
3.922AspIle: 3.922 ± 0.078
3.254AspLys: 3.254 ± 0.062
6.018AspLeu: 6.018 ± 0.092
1.408AspMet: 1.408 ± 0.035
1.558AspAsn: 1.558 ± 0.045
1.946AspPro: 1.946 ± 0.054
1.611AspGln: 1.611 ± 0.039
2.36AspArg: 2.36 ± 0.057
2.625AspSer: 2.625 ± 0.052
2.597AspThr: 2.597 ± 0.052
3.743AspVal: 3.743 ± 0.065
0.389AspTrp: 0.389 ± 0.02
2.279AspTyr: 2.279 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
5.874GluAla: 5.874 ± 0.079
0.39GluCys: 0.39 ± 0.024
5.22GluAsp: 5.22 ± 0.082
10.228GluGlu: 10.228 ± 0.164
2.8GluPhe: 2.8 ± 0.062
5.605GluGly: 5.605 ± 0.109
1.29GluHis: 1.29 ± 0.038
6.318GluIle: 6.318 ± 0.088
8.258GluLys: 8.258 ± 0.105
7.193GluLeu: 7.193 ± 0.096
2.765GluMet: 2.765 ± 0.051
4.415GluAsn: 4.415 ± 0.061
1.998GluPro: 1.998 ± 0.054
1.759GluGln: 1.759 ± 0.048
3.755GluArg: 3.755 ± 0.076
4.114GluSer: 4.114 ± 0.057
4.164GluThr: 4.164 ± 0.07
5.983GluVal: 5.983 ± 0.085
0.481GluTrp: 0.481 ± 0.023
2.784GluTyr: 2.784 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
2.482PheAla: 2.482 ± 0.055
0.318PheCys: 0.318 ± 0.018
2.309PheAsp: 2.309 ± 0.049
2.95PheGlu: 2.95 ± 0.06
2.28PhePhe: 2.28 ± 0.06
3.207PheGly: 3.207 ± 0.07
0.957PheHis: 0.957 ± 0.032
3.41PheIle: 3.41 ± 0.065
2.46PheLys: 2.46 ± 0.05
4.756PheLeu: 4.756 ± 0.092
1.537PheMet: 1.537 ± 0.041
1.761PheAsn: 1.761 ± 0.051
1.384PhePro: 1.384 ± 0.04
1.524PheGln: 1.524 ± 0.042
1.848PheArg: 1.848 ± 0.052
3.494PheSer: 3.494 ± 0.067
2.757PheThr: 2.757 ± 0.061
2.872PheVal: 2.872 ± 0.059
0.316PheTrp: 0.316 ± 0.017
1.899PheTyr: 1.899 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
4.945GlyAla: 4.945 ± 0.089
0.65GlyCys: 0.65 ± 0.028
3.352GlyAsp: 3.352 ± 0.069
5.213GlyGlu: 5.213 ± 0.085
3.447GlyPhe: 3.447 ± 0.07
4.778GlyGly: 4.778 ± 0.093
1.298GlyHis: 1.298 ± 0.041
6.291GlyIle: 6.291 ± 0.091
5.308GlyLys: 5.308 ± 0.083
6.646GlyLeu: 6.646 ± 0.099
2.214GlyMet: 2.214 ± 0.048
2.718GlyAsn: 2.718 ± 0.064
1.547GlyPro: 1.547 ± 0.041
1.786GlyGln: 1.786 ± 0.043
2.857GlyArg: 2.857 ± 0.055
4.055GlySer: 4.055 ± 0.072
4.111GlyThr: 4.111 ± 0.07
5.232GlyVal: 5.232 ± 0.086
0.642GlyTrp: 0.642 ± 0.037
3.095GlyTyr: 3.095 ± 0.059
0.0GlyXaa: 0.0 ± 0.0
His
1.086HisAla: 1.086 ± 0.034
0.168HisCys: 0.168 ± 0.016
0.947HisAsp: 0.947 ± 0.033
1.56HisGlu: 1.56 ± 0.042
0.894HisPhe: 0.894 ± 0.031
1.463HisGly: 1.463 ± 0.046
0.571HisHis: 0.571 ± 0.024
1.231HisIle: 1.231 ± 0.041
1.187HisLys: 1.187 ± 0.036
2.163HisLeu: 2.163 ± 0.052
0.574HisMet: 0.574 ± 0.025
0.613HisAsn: 0.613 ± 0.025
0.939HisPro: 0.939 ± 0.031
0.635HisGln: 0.635 ± 0.03
0.88HisArg: 0.88 ± 0.03
0.907HisSer: 0.907 ± 0.033
0.9HisThr: 0.9 ± 0.035
1.285HisVal: 1.285 ± 0.045
0.16HisTrp: 0.16 ± 0.012
0.762HisTyr: 0.762 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
4.896IleAla: 4.896 ± 0.08
0.529IleCys: 0.529 ± 0.025
3.644IleAsp: 3.644 ± 0.065
5.316IleGlu: 5.316 ± 0.087
3.226IlePhe: 3.226 ± 0.066
5.139IleGly: 5.139 ± 0.087
1.669IleHis: 1.669 ± 0.04
5.124IleIle: 5.124 ± 0.092
4.358IleLys: 4.358 ± 0.077
8.724IleLeu: 8.724 ± 0.103
1.951IleMet: 1.951 ± 0.041
2.702IleAsn: 2.702 ± 0.062
3.026IlePro: 3.026 ± 0.067
2.049IleGln: 2.049 ± 0.047
3.692IleArg: 3.692 ± 0.062
5.236IleSer: 5.236 ± 0.086
4.175IleThr: 4.175 ± 0.083
4.587IleVal: 4.587 ± 0.074
0.412IleTrp: 0.412 ± 0.02
2.63IleTyr: 2.63 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
4.635LysAla: 4.635 ± 0.074
0.328LysCys: 0.328 ± 0.02
4.795LysAsp: 4.795 ± 0.078
8.874LysGlu: 8.874 ± 0.125
1.972LysPhe: 1.972 ± 0.051
4.714LysGly: 4.714 ± 0.073
1.114LysHis: 1.114 ± 0.034
5.221LysIle: 5.221 ± 0.063
6.884LysLys: 6.884 ± 0.099
6.048LysLeu: 6.048 ± 0.103
2.251LysMet: 2.251 ± 0.049
4.149LysAsn: 4.149 ± 0.068
2.043LysPro: 2.043 ± 0.048
1.429LysGln: 1.429 ± 0.04
3.248LysArg: 3.248 ± 0.063
3.961LysSer: 3.961 ± 0.072
3.817LysThr: 3.817 ± 0.068
5.193LysVal: 5.193 ± 0.069
0.493LysTrp: 0.493 ± 0.025
2.565LysTyr: 2.565 ± 0.056
0.0LysXaa: 0.0 ± 0.0
Leu
6.226LeuAla: 6.226 ± 0.076
0.72LeuCys: 0.72 ± 0.03
5.181LeuAsp: 5.181 ± 0.077
8.039LeuGlu: 8.039 ± 0.107
4.672LeuPhe: 4.672 ± 0.083
7.223LeuGly: 7.223 ± 0.105
2.009LeuHis: 2.009 ± 0.052
6.726LeuIle: 6.726 ± 0.096
8.038LeuLys: 8.038 ± 0.115
11.587LeuLeu: 11.587 ± 0.156
3.274LeuMet: 3.274 ± 0.062
3.909LeuAsn: 3.909 ± 0.067
3.887LeuPro: 3.887 ± 0.074
3.192LeuGln: 3.192 ± 0.066
4.509LeuArg: 4.509 ± 0.084
8.25LeuSer: 8.25 ± 0.111
5.498LeuThr: 5.498 ± 0.085
6.359LeuVal: 6.359 ± 0.085
0.736LeuTrp: 0.736 ± 0.029
3.95LeuTyr: 3.95 ± 0.07
0.0LeuXaa: 0.0 ± 0.0
Met
2.398MetAla: 2.398 ± 0.056
0.143MetCys: 0.143 ± 0.013
1.989MetAsp: 1.989 ± 0.05
3.13MetGlu: 3.13 ± 0.056
0.985MetPhe: 0.985 ± 0.034
2.295MetGly: 2.295 ± 0.054
0.399MetHis: 0.399 ± 0.021
2.162MetIle: 2.162 ± 0.047
3.004MetLys: 3.004 ± 0.057
2.615MetLeu: 2.615 ± 0.054
1.011MetMet: 1.011 ± 0.039
1.713MetAsn: 1.713 ± 0.047
1.039MetPro: 1.039 ± 0.038
0.679MetGln: 0.679 ± 0.03
1.381MetArg: 1.381 ± 0.04
1.735MetSer: 1.735 ± 0.049
1.572MetThr: 1.572 ± 0.042
2.235MetVal: 2.235 ± 0.046
0.131MetTrp: 0.131 ± 0.012
0.779MetTyr: 0.779 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.614AsnAla: 2.614 ± 0.06
0.29AsnCys: 0.29 ± 0.019
1.754AsnAsp: 1.754 ± 0.047
3.056AsnGlu: 3.056 ± 0.054
1.74AsnPhe: 1.74 ± 0.04
2.903AsnGly: 2.903 ± 0.067
0.937AsnHis: 0.937 ± 0.032
3.274AsnIle: 3.274 ± 0.061
2.517AsnLys: 2.517 ± 0.057
4.614AsnLeu: 4.614 ± 0.079
1.202AsnMet: 1.202 ± 0.034
1.458AsnAsn: 1.458 ± 0.052
2.016AsnPro: 2.016 ± 0.045
1.303AsnGln: 1.303 ± 0.033
1.929AsnArg: 1.929 ± 0.047
2.088AsnSer: 2.088 ± 0.054
2.195AsnThr: 2.195 ± 0.054
2.557AsnVal: 2.557 ± 0.053
0.349AsnTrp: 0.349 ± 0.021
1.645AsnTyr: 1.645 ± 0.047
0.0AsnXaa: 0.0 ± 0.0
Pro
1.814ProAla: 1.814 ± 0.05
0.256ProCys: 0.256 ± 0.015
1.736ProAsp: 1.736 ± 0.042
3.309ProGlu: 3.309 ± 0.063
1.663ProPhe: 1.663 ± 0.04
2.392ProGly: 2.392 ± 0.057
0.685ProHis: 0.685 ± 0.026
2.138ProIle: 2.138 ± 0.054
2.186ProLys: 2.186 ± 0.051
3.47ProLeu: 3.47 ± 0.063
1.129ProMet: 1.129 ± 0.037
1.162ProAsn: 1.162 ± 0.034
0.752ProPro: 0.752 ± 0.03
0.995ProGln: 0.995 ± 0.039
1.044ProArg: 1.044 ± 0.033
2.086ProSer: 2.086 ± 0.051
1.497ProThr: 1.497 ± 0.059
3.035ProVal: 3.035 ± 0.062
0.282ProTrp: 0.282 ± 0.018
1.513ProTyr: 1.513 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
1.563GlnAla: 1.563 ± 0.039
0.187GlnCys: 0.187 ± 0.015
1.445GlnAsp: 1.445 ± 0.041
2.644GlnGlu: 2.644 ± 0.064
1.163GlnPhe: 1.163 ± 0.041
2.047GlnGly: 2.047 ± 0.052
0.434GlnHis: 0.434 ± 0.022
1.815GlnIle: 1.815 ± 0.05
2.386GlnLys: 2.386 ± 0.055
2.174GlnLeu: 2.174 ± 0.056
0.924GlnMet: 0.924 ± 0.035
1.399GlnAsn: 1.399 ± 0.041
0.697GlnPro: 0.697 ± 0.028
0.679GlnGln: 0.679 ± 0.028
1.313GlnArg: 1.313 ± 0.04
1.57GlnSer: 1.57 ± 0.043
1.175GlnThr: 1.175 ± 0.037
1.994GlnVal: 1.994 ± 0.043
0.212GlnTrp: 0.212 ± 0.015
1.097GlnTyr: 1.097 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
2.398ArgAla: 2.398 ± 0.052
0.341ArgCys: 0.341 ± 0.017
2.267ArgAsp: 2.267 ± 0.055
3.924ArgGlu: 3.924 ± 0.075
1.997ArgPhe: 1.997 ± 0.054
2.607ArgGly: 2.607 ± 0.06
0.684ArgHis: 0.684 ± 0.027
3.659ArgIle: 3.659 ± 0.071
3.763ArgLys: 3.763 ± 0.062
3.839ArgLeu: 3.839 ± 0.066
1.439ArgMet: 1.439 ± 0.037
2.197ArgAsn: 2.197 ± 0.051
1.211ArgPro: 1.211 ± 0.038
1.114ArgGln: 1.114 ± 0.036
2.191ArgArg: 2.191 ± 0.055
2.382ArgSer: 2.382 ± 0.052
2.269ArgThr: 2.269 ± 0.054
2.871ArgVal: 2.871 ± 0.067
0.345ArgTrp: 0.345 ± 0.021
1.757ArgTyr: 1.757 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
3.906SerAla: 3.906 ± 0.073
0.475SerCys: 0.475 ± 0.024
2.891SerAsp: 2.891 ± 0.057
4.303SerGlu: 4.303 ± 0.067
3.418SerPhe: 3.418 ± 0.063
4.898SerGly: 4.898 ± 0.083
1.113SerHis: 1.113 ± 0.037
4.945SerIle: 4.945 ± 0.078
3.92SerLys: 3.92 ± 0.066
6.797SerLeu: 6.797 ± 0.099
2.187SerMet: 2.187 ± 0.049
2.023SerAsn: 2.023 ± 0.051
1.997SerPro: 1.997 ± 0.05
1.621SerGln: 1.621 ± 0.04
2.443SerArg: 2.443 ± 0.057
4.213SerSer: 4.213 ± 0.078
3.084SerThr: 3.084 ± 0.051
4.514SerVal: 4.514 ± 0.07
0.451SerTrp: 0.451 ± 0.026
2.917SerTyr: 2.917 ± 0.06
0.0SerXaa: 0.0 ± 0.0
Thr
3.52ThrAla: 3.52 ± 0.07
0.308ThrCys: 0.308 ± 0.016
2.475ThrAsp: 2.475 ± 0.055
3.883ThrGlu: 3.883 ± 0.069
2.425ThrPhe: 2.425 ± 0.056
4.293ThrGly: 4.293 ± 0.068
0.934ThrHis: 0.934 ± 0.031
3.776ThrIle: 3.776 ± 0.067
3.319ThrLys: 3.319 ± 0.063
5.972ThrLeu: 5.972 ± 0.081
1.605ThrMet: 1.605 ± 0.036
1.709ThrAsn: 1.709 ± 0.044
2.282ThrPro: 2.282 ± 0.062
1.207ThrGln: 1.207 ± 0.036
1.978ThrArg: 1.978 ± 0.038
3.126ThrSer: 3.126 ± 0.064
2.644ThrThr: 2.644 ± 0.058
4.221ThrVal: 4.221 ± 0.082
0.41ThrTrp: 0.41 ± 0.023
2.14ThrTyr: 2.14 ± 0.053
0.0ThrXaa: 0.0 ± 0.0
Val
4.281ValAla: 4.281 ± 0.074
0.519ValCys: 0.519 ± 0.022
3.952ValAsp: 3.952 ± 0.069
5.672ValGlu: 5.672 ± 0.086
3.329ValPhe: 3.329 ± 0.062
4.2ValGly: 4.2 ± 0.074
1.43ValHis: 1.43 ± 0.041
4.901ValIle: 4.901 ± 0.071
5.007ValLys: 5.007 ± 0.087
8.233ValLeu: 8.233 ± 0.1
2.196ValMet: 2.196 ± 0.05
2.757ValAsn: 2.757 ± 0.049
2.537ValPro: 2.537 ± 0.053
1.787ValGln: 1.787 ± 0.045
2.801ValArg: 2.801 ± 0.063
4.955ValSer: 4.955 ± 0.084
3.999ValThr: 3.999 ± 0.078
4.979ValVal: 4.979 ± 0.098
0.424ValTrp: 0.424 ± 0.023
2.653ValTyr: 2.653 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.442TrpAla: 0.442 ± 0.024
0.058TrpCys: 0.058 ± 0.007
0.366TrpAsp: 0.366 ± 0.019
0.446TrpGlu: 0.446 ± 0.022
0.35TrpPhe: 0.35 ± 0.022
0.483TrpGly: 0.483 ± 0.025
0.132TrpHis: 0.132 ± 0.013
0.557TrpIle: 0.557 ± 0.025
0.515TrpLys: 0.515 ± 0.023
0.618TrpLeu: 0.618 ± 0.029
0.264TrpMet: 0.264 ± 0.018
0.382TrpAsn: 0.382 ± 0.023
0.21TrpPro: 0.21 ± 0.015
0.239TrpGln: 0.239 ± 0.017
0.283TrpArg: 0.283 ± 0.018
0.472TrpSer: 0.472 ± 0.022
0.404TrpThr: 0.404 ± 0.023
0.436TrpVal: 0.436 ± 0.023
0.06TrpTrp: 0.06 ± 0.007
0.272TrpTyr: 0.272 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.426TyrAla: 2.426 ± 0.046
0.307TyrCys: 0.307 ± 0.019
2.121TyrAsp: 2.121 ± 0.044
3.373TyrGlu: 3.373 ± 0.068
1.983TyrPhe: 1.983 ± 0.053
2.892TyrGly: 2.892 ± 0.053
0.82TyrHis: 0.82 ± 0.031
2.547TyrIle: 2.547 ± 0.051
2.427TyrLys: 2.427 ± 0.049
4.137TyrLeu: 4.137 ± 0.064
0.993TyrMet: 0.993 ± 0.033
1.533TyrAsn: 1.533 ± 0.048
1.432TyrPro: 1.432 ± 0.041
1.379TyrGln: 1.379 ± 0.048
1.939TyrArg: 1.939 ± 0.05
2.314TyrSer: 2.314 ± 0.052
2.12TyrThr: 2.12 ± 0.057
2.625TyrVal: 2.625 ± 0.057
0.269TyrTrp: 0.269 ± 0.017
1.838TyrTyr: 1.838 ± 0.053
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2937 proteins (937308 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski