Amino acid dipepetide frequency for Synechococcus phage S-CAM7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.743AlaAla: 5.743 ± 0.572
0.574AlaCys: 0.574 ± 0.117
4.126AlaAsp: 4.126 ± 0.308
4.096AlaGlu: 4.096 ± 0.341
2.267AlaPhe: 2.267 ± 0.172
5.426AlaGly: 5.426 ± 0.409
0.937AlaHis: 0.937 ± 0.155
4.292AlaIle: 4.292 ± 0.2
3.627AlaLys: 3.627 ± 0.46
4.731AlaLeu: 4.731 ± 0.398
1.134AlaMet: 1.134 ± 0.176
3.567AlaAsn: 3.567 ± 0.268
2.705AlaPro: 2.705 ± 0.22
2.63AlaGln: 2.63 ± 0.256
2.63AlaArg: 2.63 ± 0.263
5.517AlaSer: 5.517 ± 0.383
7.119AlaThr: 7.119 ± 1.297
4.292AlaVal: 4.292 ± 0.278
0.514AlaTrp: 0.514 ± 0.089
2.161AlaTyr: 2.161 ± 0.181
0.0AlaXaa: 0.0 ± 0.0
Cys
0.65CysAla: 0.65 ± 0.104
0.106CysCys: 0.106 ± 0.038
0.816CysAsp: 0.816 ± 0.125
0.62CysGlu: 0.62 ± 0.099
0.212CysPhe: 0.212 ± 0.058
0.469CysGly: 0.469 ± 0.098
0.106CysHis: 0.106 ± 0.045
0.574CysIle: 0.574 ± 0.115
0.469CysLys: 0.469 ± 0.109
0.635CysLeu: 0.635 ± 0.136
0.242CysMet: 0.242 ± 0.064
0.499CysAsn: 0.499 ± 0.094
0.544CysPro: 0.544 ± 0.192
0.242CysGln: 0.242 ± 0.066
0.453CysArg: 0.453 ± 0.087
0.559CysSer: 0.559 ± 0.112
0.469CysThr: 0.469 ± 0.084
0.725CysVal: 0.725 ± 0.144
0.076CysTrp: 0.076 ± 0.04
0.393CysTyr: 0.393 ± 0.083
0.0CysXaa: 0.0 ± 0.0
Asp
4.489AspAla: 4.489 ± 0.318
0.786AspCys: 0.786 ± 0.148
4.066AspAsp: 4.066 ± 0.244
3.733AspGlu: 3.733 ± 0.253
3.431AspPhe: 3.431 ± 0.293
5.668AspGly: 5.668 ± 0.378
0.665AspHis: 0.665 ± 0.099
5.26AspIle: 5.26 ± 0.345
2.645AspLys: 2.645 ± 0.276
5.486AspLeu: 5.486 ± 0.428
1.103AspMet: 1.103 ± 0.144
3.658AspAsn: 3.658 ± 0.431
2.811AspPro: 2.811 ± 0.195
1.98AspGln: 1.98 ± 0.239
2.932AspArg: 2.932 ± 0.224
4.761AspSer: 4.761 ± 0.352
4.292AspThr: 4.292 ± 0.323
4.368AspVal: 4.368 ± 0.342
0.786AspTrp: 0.786 ± 0.134
3.083AspTyr: 3.083 ± 0.235
0.0AspXaa: 0.0 ± 0.0
Glu
4.338GluAla: 4.338 ± 0.383
0.68GluCys: 0.68 ± 0.103
3.763GluAsp: 3.763 ± 0.27
4.398GluGlu: 4.398 ± 0.501
3.083GluPhe: 3.083 ± 0.2
4.247GluGly: 4.247 ± 0.324
0.922GluHis: 0.922 ± 0.139
3.884GluIle: 3.884 ± 0.324
3.355GluLys: 3.355 ± 0.39
5.456GluLeu: 5.456 ± 0.373
1.36GluMet: 1.36 ± 0.225
3.189GluAsn: 3.189 ± 0.265
1.799GluPro: 1.799 ± 0.19
2.146GluGln: 2.146 ± 0.241
2.705GluArg: 2.705 ± 0.264
4.051GluSer: 4.051 ± 0.277
3.658GluThr: 3.658 ± 0.272
4.882GluVal: 4.882 ± 0.252
0.741GluTrp: 0.741 ± 0.134
2.781GluTyr: 2.781 ± 0.238
0.0GluXaa: 0.0 ± 0.0
Phe
2.63PheAla: 2.63 ± 0.212
0.544PheCys: 0.544 ± 0.108
3.612PheAsp: 3.612 ± 0.263
2.736PheGlu: 2.736 ± 0.262
1.678PhePhe: 1.678 ± 0.176
2.645PheGly: 2.645 ± 0.222
0.469PheHis: 0.469 ± 0.087
2.66PheIle: 2.66 ± 0.208
1.874PheLys: 1.874 ± 0.229
2.826PheLeu: 2.826 ± 0.214
0.725PheMet: 0.725 ± 0.124
2.796PheAsn: 2.796 ± 0.192
1.919PhePro: 1.919 ± 0.438
1.632PheGln: 1.632 ± 0.16
1.663PheArg: 1.663 ± 0.144
3.552PheSer: 3.552 ± 0.212
3.416PheThr: 3.416 ± 0.3
3.491PheVal: 3.491 ± 0.273
0.363PheTrp: 0.363 ± 0.08
1.768PheTyr: 1.768 ± 0.162
0.0PheXaa: 0.0 ± 0.0
Gly
7.95GlyAla: 7.95 ± 1.433
0.438GlyCys: 0.438 ± 0.094
6.469GlyAsp: 6.469 ± 0.579
4.7GlyGlu: 4.7 ± 0.258
3.37GlyPhe: 3.37 ± 0.268
7.557GlyGly: 7.557 ± 0.686
0.892GlyHis: 0.892 ± 0.165
6.756GlyIle: 6.756 ± 0.689
3.325GlyLys: 3.325 ± 0.379
5.789GlyLeu: 5.789 ± 0.499
1.421GlyMet: 1.421 ± 0.192
4.368GlyAsn: 4.368 ± 0.31
3.854GlyPro: 3.854 ± 0.919
2.176GlyGln: 2.176 ± 0.179
2.569GlyArg: 2.569 ± 0.204
7.104GlySer: 7.104 ± 0.621
6.348GlyThr: 6.348 ± 0.574
5.789GlyVal: 5.789 ± 0.523
0.846GlyTrp: 0.846 ± 0.113
2.917GlyTyr: 2.917 ± 0.256
0.0GlyXaa: 0.0 ± 0.0
His
0.695HisAla: 0.695 ± 0.118
0.136HisCys: 0.136 ± 0.042
0.771HisAsp: 0.771 ± 0.116
0.695HisGlu: 0.695 ± 0.136
0.65HisPhe: 0.65 ± 0.099
0.967HisGly: 0.967 ± 0.147
0.423HisHis: 0.423 ± 0.115
0.937HisIle: 0.937 ± 0.153
0.695HisLys: 0.695 ± 0.112
1.118HisLeu: 1.118 ± 0.188
0.242HisMet: 0.242 ± 0.072
0.892HisAsn: 0.892 ± 0.169
0.635HisPro: 0.635 ± 0.105
0.438HisGln: 0.438 ± 0.082
0.529HisArg: 0.529 ± 0.098
1.179HisSer: 1.179 ± 0.137
1.149HisThr: 1.149 ± 0.265
0.665HisVal: 0.665 ± 0.122
0.181HisTrp: 0.181 ± 0.056
0.816HisTyr: 0.816 ± 0.134
0.0HisXaa: 0.0 ± 0.0
Ile
4.428IleAla: 4.428 ± 0.283
0.469IleCys: 0.469 ± 0.109
5.139IleAsp: 5.139 ± 0.485
4.428IleGlu: 4.428 ± 0.318
2.751IlePhe: 2.751 ± 0.352
5.441IleGly: 5.441 ± 0.529
0.741IleHis: 0.741 ± 0.122
4.353IleIle: 4.353 ± 0.353
3.99IleLys: 3.99 ± 0.263
4.307IleLeu: 4.307 ± 0.285
1.36IleMet: 1.36 ± 0.197
3.99IleAsn: 3.99 ± 0.241
2.509IlePro: 2.509 ± 0.17
2.539IleGln: 2.539 ± 0.261
3.113IleArg: 3.113 ± 0.195
5.381IleSer: 5.381 ± 0.4
5.819IleThr: 5.819 ± 0.467
4.64IleVal: 4.64 ± 0.325
0.393IleTrp: 0.393 ± 0.079
2.086IleTyr: 2.086 ± 0.182
0.0IleXaa: 0.0 ± 0.0
Lys
3.355LysAla: 3.355 ± 0.366
0.408LysCys: 0.408 ± 0.08
2.675LysAsp: 2.675 ± 0.317
3.537LysGlu: 3.537 ± 0.403
2.222LysPhe: 2.222 ± 0.206
2.887LysGly: 2.887 ± 0.322
0.861LysHis: 0.861 ± 0.139
3.159LysIle: 3.159 ± 0.303
3.794LysLys: 3.794 ± 0.483
4.277LysLeu: 4.277 ± 0.301
1.451LysMet: 1.451 ± 0.216
2.721LysAsn: 2.721 ± 0.312
2.04LysPro: 2.04 ± 0.254
1.663LysGln: 1.663 ± 0.184
2.146LysArg: 2.146 ± 0.316
2.947LysSer: 2.947 ± 0.289
3.159LysThr: 3.159 ± 0.306
3.718LysVal: 3.718 ± 0.248
0.559LysTrp: 0.559 ± 0.13
2.101LysTyr: 2.101 ± 0.213
0.0LysXaa: 0.0 ± 0.0
Leu
4.171LeuAla: 4.171 ± 0.371
0.741LeuCys: 0.741 ± 0.141
5.426LeuAsp: 5.426 ± 0.324
5.124LeuGlu: 5.124 ± 0.343
3.008LeuPhe: 3.008 ± 0.217
4.746LeuGly: 4.746 ± 0.268
1.164LeuHis: 1.164 ± 0.197
4.217LeuIle: 4.217 ± 0.298
3.93LeuLys: 3.93 ± 0.412
5.063LeuLeu: 5.063 ± 0.363
1.723LeuMet: 1.723 ± 0.254
4.731LeuAsn: 4.731 ± 0.287
3.219LeuPro: 3.219 ± 0.277
2.69LeuGln: 2.69 ± 0.198
3.763LeuArg: 3.763 ± 0.232
5.758LeuSer: 5.758 ± 0.339
6.287LeuThr: 6.287 ± 0.526
4.761LeuVal: 4.761 ± 0.317
0.574LeuTrp: 0.574 ± 0.106
2.917LeuTyr: 2.917 ± 0.223
0.0LeuXaa: 0.0 ± 0.0
Met
1.602MetAla: 1.602 ± 0.246
0.257MetCys: 0.257 ± 0.075
1.254MetAsp: 1.254 ± 0.172
1.421MetGlu: 1.421 ± 0.228
0.514MetPhe: 0.514 ± 0.094
1.149MetGly: 1.149 ± 0.176
0.378MetHis: 0.378 ± 0.08
1.013MetIle: 1.013 ± 0.182
1.406MetLys: 1.406 ± 0.221
1.285MetLeu: 1.285 ± 0.2
0.317MetMet: 0.317 ± 0.082
0.982MetAsn: 0.982 ± 0.169
0.801MetPro: 0.801 ± 0.129
0.589MetGln: 0.589 ± 0.105
0.846MetArg: 0.846 ± 0.143
1.587MetSer: 1.587 ± 0.18
1.239MetThr: 1.239 ± 0.171
1.149MetVal: 1.149 ± 0.17
0.227MetTrp: 0.227 ± 0.075
0.725MetTyr: 0.725 ± 0.14
0.0MetXaa: 0.0 ± 0.0
Asn
3.28AsnAla: 3.28 ± 0.255
0.348AsnCys: 0.348 ± 0.09
3.174AsnAsp: 3.174 ± 0.201
2.993AsnGlu: 2.993 ± 0.236
2.826AsnPhe: 2.826 ± 0.202
4.504AsnGly: 4.504 ± 0.321
0.907AsnHis: 0.907 ± 0.122
4.64AsnIle: 4.64 ± 0.284
2.494AsnLys: 2.494 ± 0.247
5.033AsnLeu: 5.033 ± 0.322
0.756AsnMet: 0.756 ± 0.13
3.386AsnAsn: 3.386 ± 0.313
3.204AsnPro: 3.204 ± 0.252
2.116AsnGln: 2.116 ± 0.192
1.98AsnArg: 1.98 ± 0.146
4.413AsnSer: 4.413 ± 0.359
3.839AsnThr: 3.839 ± 0.273
4.942AsnVal: 4.942 ± 0.638
0.484AsnTrp: 0.484 ± 0.078
2.207AsnTyr: 2.207 ± 0.189
0.0AsnXaa: 0.0 ± 0.0
Pro
2.66ProAla: 2.66 ± 0.202
0.181ProCys: 0.181 ± 0.053
2.66ProAsp: 2.66 ± 0.275
2.902ProGlu: 2.902 ± 0.215
1.511ProPhe: 1.511 ± 0.155
3.794ProGly: 3.794 ± 0.537
0.907ProHis: 0.907 ± 0.252
2.539ProIle: 2.539 ± 0.261
1.965ProLys: 1.965 ± 0.225
2.615ProLeu: 2.615 ± 0.217
0.62ProMet: 0.62 ± 0.125
2.222ProAsn: 2.222 ± 0.223
1.632ProPro: 1.632 ± 0.208
2.071ProGln: 2.071 ± 0.35
1.481ProArg: 1.481 ± 0.162
3.31ProSer: 3.31 ± 0.29
3.129ProThr: 3.129 ± 0.258
2.358ProVal: 2.358 ± 0.232
0.453ProTrp: 0.453 ± 0.1
1.466ProTyr: 1.466 ± 0.16
0.0ProXaa: 0.0 ± 0.0
Gln
2.328GlnAla: 2.328 ± 0.238
0.363GlnCys: 0.363 ± 0.069
1.829GlnAsp: 1.829 ± 0.165
2.207GlnGlu: 2.207 ± 0.205
1.844GlnPhe: 1.844 ± 0.167
3.098GlnGly: 3.098 ± 0.652
0.438GlnHis: 0.438 ± 0.106
2.116GlnIle: 2.116 ± 0.216
1.572GlnLys: 1.572 ± 0.205
3.023GlnLeu: 3.023 ± 0.204
0.846GlnMet: 0.846 ± 0.141
1.647GlnAsn: 1.647 ± 0.163
1.285GlnPro: 1.285 ± 0.159
1.375GlnGln: 1.375 ± 0.198
1.39GlnArg: 1.39 ± 0.167
2.267GlnSer: 2.267 ± 0.201
1.98GlnThr: 1.98 ± 0.173
2.872GlnVal: 2.872 ± 0.222
0.408GlnTrp: 0.408 ± 0.112
1.451GlnTyr: 1.451 ± 0.168
0.0GlnXaa: 0.0 ± 0.0
Arg
2.358ArgAla: 2.358 ± 0.226
0.453ArgCys: 0.453 ± 0.085
2.69ArgAsp: 2.69 ± 0.186
2.751ArgGlu: 2.751 ± 0.291
1.98ArgPhe: 1.98 ± 0.152
3.506ArgGly: 3.506 ± 0.219
0.499ArgHis: 0.499 ± 0.097
2.569ArgIle: 2.569 ± 0.183
2.388ArgLys: 2.388 ± 0.323
3.083ArgLeu: 3.083 ± 0.211
1.028ArgMet: 1.028 ± 0.186
2.025ArgAsn: 2.025 ± 0.211
1.406ArgPro: 1.406 ± 0.16
1.254ArgGln: 1.254 ± 0.137
1.98ArgArg: 1.98 ± 0.245
2.358ArgSer: 2.358 ± 0.239
2.705ArgThr: 2.705 ± 0.22
3.038ArgVal: 3.038 ± 0.238
0.333ArgTrp: 0.333 ± 0.069
2.071ArgTyr: 2.071 ± 0.208
0.0ArgXaa: 0.0 ± 0.0
Ser
4.791SerAla: 4.791 ± 0.359
0.635SerCys: 0.635 ± 0.131
4.595SerAsp: 4.595 ± 0.37
4.126SerGlu: 4.126 ± 0.328
3.416SerPhe: 3.416 ± 0.264
7.572SerGly: 7.572 ± 0.607
0.952SerHis: 0.952 ± 0.137
5.789SerIle: 5.789 ± 0.442
3.401SerLys: 3.401 ± 0.287
5.638SerLeu: 5.638 ± 0.342
1.436SerMet: 1.436 ± 0.211
4.398SerAsn: 4.398 ± 0.287
2.962SerPro: 2.962 ± 0.26
1.965SerGln: 1.965 ± 0.159
2.736SerArg: 2.736 ± 0.221
6.212SerSer: 6.212 ± 0.458
6.983SerThr: 6.983 ± 0.583
4.806SerVal: 4.806 ± 0.376
0.469SerTrp: 0.469 ± 0.087
2.6SerTyr: 2.6 ± 0.197
0.0SerXaa: 0.0 ± 0.0
Thr
4.7ThrAla: 4.7 ± 0.334
0.529ThrCys: 0.529 ± 0.093
4.489ThrAsp: 4.489 ± 0.319
3.673ThrGlu: 3.673 ± 0.29
3.718ThrPhe: 3.718 ± 0.499
10.852ThrGly: 10.852 ± 2.266
0.952ThrHis: 0.952 ± 0.133
5.199ThrIle: 5.199 ± 0.389
3.098ThrLys: 3.098 ± 0.274
5.955ThrLeu: 5.955 ± 0.397
0.922ThrMet: 0.922 ± 0.121
4.519ThrAsn: 4.519 ± 0.421
3.204ThrPro: 3.204 ± 0.257
2.841ThrGln: 2.841 ± 0.235
2.222ThrArg: 2.222 ± 0.202
5.426ThrSer: 5.426 ± 0.38
6.212ThrThr: 6.212 ± 0.715
5.486ThrVal: 5.486 ± 0.579
0.559ThrTrp: 0.559 ± 0.088
2.705ThrTyr: 2.705 ± 0.331
0.0ThrXaa: 0.0 ± 0.0
Val
4.791ValAla: 4.791 ± 0.33
0.544ValCys: 0.544 ± 0.106
4.444ValAsp: 4.444 ± 0.247
4.459ValGlu: 4.459 ± 0.319
2.63ValPhe: 2.63 ± 0.225
6.62ValGly: 6.62 ± 0.838
0.816ValHis: 0.816 ± 0.119
4.912ValIle: 4.912 ± 0.345
3.295ValLys: 3.295 ± 0.262
4.474ValLeu: 4.474 ± 0.338
1.103ValMet: 1.103 ± 0.158
4.459ValAsn: 4.459 ± 0.321
2.584ValPro: 2.584 ± 0.222
2.328ValGln: 2.328 ± 0.159
3.008ValArg: 3.008 ± 0.176
5.955ValSer: 5.955 ± 0.547
6.166ValThr: 6.166 ± 0.689
4.474ValVal: 4.474 ± 0.376
0.393ValTrp: 0.393 ± 0.077
2.766ValTyr: 2.766 ± 0.333
0.0ValXaa: 0.0 ± 0.0
Trp
0.605TrpAla: 0.605 ± 0.115
0.121TrpCys: 0.121 ± 0.045
0.695TrpAsp: 0.695 ± 0.128
0.725TrpGlu: 0.725 ± 0.135
0.438TrpPhe: 0.438 ± 0.107
0.741TrpGly: 0.741 ± 0.123
0.151TrpHis: 0.151 ± 0.051
0.484TrpIle: 0.484 ± 0.096
0.559TrpLys: 0.559 ± 0.091
0.635TrpLeu: 0.635 ± 0.122
0.212TrpMet: 0.212 ± 0.066
0.68TrpAsn: 0.68 ± 0.121
0.0TrpPro: 0.0 ± 0.0
0.227TrpGln: 0.227 ± 0.062
0.378TrpArg: 0.378 ± 0.075
0.529TrpSer: 0.529 ± 0.102
0.62TrpThr: 0.62 ± 0.124
0.68TrpVal: 0.68 ± 0.132
0.136TrpTrp: 0.136 ± 0.047
0.302TrpTyr: 0.302 ± 0.082
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.524TyrAla: 2.524 ± 0.189
0.544TyrCys: 0.544 ± 0.11
3.31TyrAsp: 3.31 ± 0.329
2.116TyrGlu: 2.116 ± 0.182
1.451TyrPhe: 1.451 ± 0.204
2.751TyrGly: 2.751 ± 0.222
0.635TyrHis: 0.635 ± 0.116
2.524TyrIle: 2.524 ± 0.222
1.799TyrLys: 1.799 ± 0.213
2.584TyrLeu: 2.584 ± 0.255
0.725TyrMet: 0.725 ± 0.148
2.811TyrAsn: 2.811 ± 0.235
1.36TyrPro: 1.36 ± 0.129
1.436TyrGln: 1.436 ± 0.156
1.95TyrArg: 1.95 ± 0.177
2.615TyrSer: 2.615 ± 0.21
2.675TyrThr: 2.675 ± 0.237
3.083TyrVal: 3.083 ± 0.242
0.423TyrTrp: 0.423 ± 0.09
1.844TyrTyr: 1.844 ± 0.206
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 266 proteins (66165 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski