Streptococcus phage Javan278

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AWW0|A0A4D6AWW0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan278 OX=2548091 GN=Javan278_0037 PE=4 SV=1
MM1 pKa = 7.29SRR3 pKa = 11.84IEE5 pKa = 3.93SSIAEE10 pKa = 4.14MRR12 pKa = 11.84RR13 pKa = 11.84LQAIPVHH20 pKa = 7.21YY21 pKa = 10.96DD22 pKa = 2.56MGDD25 pKa = 3.2RR26 pKa = 11.84YY27 pKa = 11.27GNDD30 pKa = 3.11ADD32 pKa = 3.64GDD34 pKa = 3.77EE35 pKa = 4.77RR36 pKa = 11.84IEE38 pKa = 4.08YY39 pKa = 9.82DD40 pKa = 3.4CSSSVSQALGLSLNNNTEE58 pKa = 4.33TLKK61 pKa = 11.4SNLPKK66 pKa = 10.19INYY69 pKa = 7.9NCVYY73 pKa = 10.38DD74 pKa = 4.46GVDD77 pKa = 3.32GSIDD81 pKa = 3.6FEE83 pKa = 4.67YY84 pKa = 11.16GDD86 pKa = 3.68VAIWSPRR93 pKa = 11.84DD94 pKa = 3.44GSSSLGAFGHH104 pKa = 4.99VVIMTGPTTMIHH116 pKa = 6.52CSYY119 pKa = 10.39GYY121 pKa = 10.67DD122 pKa = 3.65GIVEE126 pKa = 4.08SDD128 pKa = 3.82YY129 pKa = 11.11NQIWEE134 pKa = 4.27LNGRR138 pKa = 11.84PRR140 pKa = 11.84EE141 pKa = 4.09TVFRR145 pKa = 11.84EE146 pKa = 3.83NGQAAAPQPAPLTNKK161 pKa = 9.79SQNLKK166 pKa = 10.0IYY168 pKa = 10.5RR169 pKa = 11.84VDD171 pKa = 3.98DD172 pKa = 3.84LQFVNGIWQVRR183 pKa = 11.84CNDD186 pKa = 3.84LVPVEE191 pKa = 5.35FDD193 pKa = 2.83WTDD196 pKa = 2.99NGIACEE202 pKa = 5.02DD203 pKa = 3.48IDD205 pKa = 5.68LVDD208 pKa = 3.74EE209 pKa = 4.77NGNLLADD216 pKa = 3.58QEE218 pKa = 4.52TRR220 pKa = 11.84AGSLFVINPNKK231 pKa = 9.88VVSDD235 pKa = 3.89GEE237 pKa = 4.24GAYY240 pKa = 10.46GSGNYY245 pKa = 7.3YY246 pKa = 8.19WRR248 pKa = 11.84NVEE251 pKa = 4.21LSASGRR257 pKa = 11.84VWLSVWSVDD266 pKa = 4.32HH267 pKa = 6.81LLHH270 pKa = 6.9GG271 pKa = 4.49

Molecular weight: 30.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B1G5|A0A4D6B1G5_9CAUD HNH homing endonuclease OS=Streptococcus phage Javan278 OX=2548091 GN=Javan278_0024 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.47FKK4 pKa = 11.11LNRR7 pKa = 11.84AGVADD12 pKa = 4.27LMKK15 pKa = 10.38SGAMQTVLNKK25 pKa = 10.0HH26 pKa = 6.06ASNIKK31 pKa = 9.22NRR33 pKa = 11.84CGDD36 pKa = 3.36GYY38 pKa = 10.92EE39 pKa = 3.66QDD41 pKa = 3.71VYY43 pKa = 11.4VGRR46 pKa = 11.84NRR48 pKa = 11.84ANAMVSAKK56 pKa = 6.74TTKK59 pKa = 10.26AKK61 pKa = 10.39KK62 pKa = 10.37DD63 pKa = 3.41NLKK66 pKa = 11.11NNTLLKK72 pKa = 10.42AVRR75 pKa = 3.79

Molecular weight: 8.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

10208

37

1368

208.3

23.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.946 ± 0.589

0.764 ± 0.136

6.23 ± 0.312

6.985 ± 0.411

4.212 ± 0.247

6.593 ± 0.511

1.528 ± 0.218

7.024 ± 0.472

8.63 ± 0.448

8.023 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.194 ± 0.188

6.132 ± 0.26

2.87 ± 0.241

4.026 ± 0.225

4.545 ± 0.349

5.956 ± 0.348

5.408 ± 0.383

6.563 ± 0.259

1.567 ± 0.147

3.801 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski